Factsheet for Os08t0178700-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
cytosol 2
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, nucleus, plastid
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	cytosol

plasma membrane: 27800704
plasma membrane: 28056797

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d024212_P013	Maize	cytosol	86.45	86.8
EES14649	Sorghum	cytosol	84.86	84.86
TraesCS7B01G293200.1	Wheat	cytosol	80.48	81.45
Os04t0634700-01	Rice	plasma membrane	80.88	81.36
TraesCS7D01G387000.1	Wheat	cytosol	80.08	81.21
HORVU7Hr1G092530.1	Barley	cytosol	80.88	80.88
Zm00001d018608_P001	Maize	cytosol	28.09	80.11
Zm00001d033807_P002	Maize	cytosol	27.89	79.55
GSMUA_Achr11P...	Banana	cytosol	72.31	75.31
GSMUA_Achr5P26320_001	Banana	cytosol	72.31	74.08
PGSC0003DMT400055773	Potato	mitochondrion	17.73	72.36
Solyc10g008640.2.1	Tomato	nucleus	70.32	71.6
KRH26885	Soybean	cytosol	68.92	70.9
PGSC0003DMT400047319	Potato	cytosol	26.69	70.9
VIT_12s0057g00010.t01	Wine grape	cytosol	70.32	70.6
KRH56049	Soybean	cytosol	67.53	70.04
Bra031148.1-P	Field mustard	cytosol	66.73	69.65
CDY39782	Canola	cytosol	67.53	69.33
Bra036672.1-P	Field mustard	cytosol	67.13	68.92
CDY03298	Canola	cytosol	66.93	68.71
CDY13851	Canola	cytosol	66.33	67.41
CDX81989	Canola	cytosol	65.74	67.21
PGSC0003DMT400047320	Potato	cytosol	38.45	66.55
AT2G20900.1	Thale cress	cytosol	66.73	65.82
KRH25479	Soybean	mitochondrion	30.88	65.4
Solyc07g054830.2.1	Tomato	cytosol	66.53	65.36
KRH22465	Soybean	mitochondrion	49.4	62.94
AT4G28130.1	Thale cress	cytosol	58.37	62.88
Os01t0783200-02	Rice	cytosol	60.76	62.63
PGSC0003DMT400039153	Potato	cytosol	44.42	62.12
CDY29883	Canola	cytosol	56.57	61.87
Bra024223.1-P	Field mustard	cytosol	56.57	61.87
CDY58713	Canola	cytosol	56.97	57.78
Os02t0787800-01	Rice	plasma membrane	29.48	30.33
Os12t0224000-01	Rice	cytosol	12.35	29.25
Os03t0425300-01	Rice	endoplasmic reticulum, golgi, nucleus	4.38	5.2

Protein Annotations

KEGG:00561+2.7.1.107	KEGG:00564+2.7.1.107	KEGG:04070+2.7.1.107	Gene3D:2.60.200.40	Gene3D:3.40.50.10330	EntrezGene:4344812
MapMan:5.2.1.4	EMBL:AK066600	InterPro:ATP-NAD_kinase_N	ProteinID:BAF23044.1	ProteinID:BAT04093.1	InterPro:DGK
InterPro:Diacylglycerol_kin_accessory	InterPro:Diacylglycerol_kinase_cat_dom	InterPro:Diacylglycerol_kinase_pln	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0003951	GO:GO:0004143	GO:GO:0005488	GO:GO:0005524	GO:GO:0007154	GO:GO:0007165
GO:GO:0007205	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016310
GO:GO:0016740	InterPro:IPR001206	InterPro:IPR017438	InterPro:NAD/diacylglycerol_kinase_sf	EnsemblPlantsGene:Os08g0178700	EnsemblPlants:Os08t0178700-01
PFAM:PF00609	PFAM:PF00781	PIRSF:PIRSF030829	PFscan:PS50146	PANTHER:PTHR11255	PANTHER:PTHR11255:SF47
UniProt:Q0J7M1	SMART:SM00045	SMART:SM00046	SUPFAM:SSF111331	UniParc:UPI0000DD93F5	RefSeq:XP_015648118.1
SEG:seg	:	:	:	:	:

Description

Similar to Calmodulin-binding diacylglycerol kinase. (Os08t0178700-01)

Coordinates

chr8:-:4599751..4604714

Molecular Weight (calculated)

55857.3 Da

IEP (calculated)

6.649

GRAVY (calculated)

-0.444

Length

502 amino acids

Sequence
(BLAST)

001: MEGEMDTVVG SSSKPCGPLT DYRIPDYILR PDAQRVLLDH APPCPVLVFI NSGSGGQLGS SLIKTYRELL GEAQVFDVSE EAPDKVLHRL NVNLEKLKMD
101: GDILAVQIWR TLRIIVAGGD GTASWLLGVV SDLKLSHPPP IATVPLGTGN NLPFSFGWGK KNPCTDQESV KSFLGLVRHA KEMKIDSWHI MLRMRATKEG
201: PCDPIAPLEL PHSLHAFHRV SSSDSLNMEG YHTFRGGFWN YFSMGMDAEI SYGFHSERKK NPEKFKNQLT NQGTYAKVGL KQGWFCASLS HPSSRNIAQL
301: ASVKIMKRAG SHWQELNIHH SIRSIVCLNL PSFSGGLNPW GTPGTRKVEE RELTAPFVDD RLIEVVGFRD AWHGLVLLAP NGHGTRLAQA QRIRFEFHKG
401: AAEHTFMRID GEPWKQPLPK DDDTVVVEIS HLGQVTMLAN EPCKSKSVHD DQSSHAQHSG HGNNDDDKDS MEDEDEWEDG RRKFGAADTF KIPDEVDIAH
501: LS

Best Arabidopsis Sequence Match ( AT2G20900.3 )
(BLAST)

001: MEKYNSLSDF LKEFYIPTYV LSAETEEEEE EESRPTPASP VLVFINSKSG GQLGGELILT YRSLLNHNQV FDLDQETPDK VLRRIYLNLE RLKDDDFARQ
101: IREKLKIIVA GGDGTAGWLL GVVCDLKLSH PPPIATVPLG TGNNLPFAFG WGKKNPGTDR TAVESFLEQV LKAKVMKIDN WHILMRMKTP KEGGSCDPVA
201: PLELPHSLHA FHRVSPTDEL NKEGCHTFRG GFWNYFSLGM DAQISYAFHS ERKLHPEKFK NQLVNQSTYV KLGCTQGWFC ASLFHPASRN IAQLAKVKIA
301: TRNGQWQDLH IPHSIRSIVC LNLPSFSGGL NPWGTPNPRK QRDRGLTPPF VDDGLIEVVG FRNAWHGLVL LAPNGHGTRL AQANRIRFEF HKGATDHTFM
401: RMDGEPWKQP LPLDDETVMV EISHLGQVNM LATHDCRSRS VFDPSTPRHQ DGAEDYDDNE DDSVAEGEEF RKFGAADTFK IPDEVDISQL S

Arabidopsis Description

DGK5Diacylglycerol kinase 5 [Source:UniProtKB/Swiss-Prot;Acc:Q9C5E5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.