Subcellular Localization
min:
: max
Winner_takes_all: cytosol
Predictor Summary:
Predictor Summary:
- cytosol 2
- mitochondrion 1
- plasma membrane 1
Predictors | GFP | MS/MS | Papers | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
PPI
No PPI Data
Homology
Paralog
locus | Identity | Homology Identity |
---|
Ortholog
locus | Homology Species | Location | Identity | Homology Identity |
---|---|---|---|---|
KXG37740 | Sorghum | cytosol | 95.97 | 97.31 |
Os03t0711200-01 | Rice | plasma membrane | 27.35 | 91.31 |
TraesCS4B01G045900.1 | Wheat | cytosol | 87.35 | 89.03 |
TraesCS4D01G046100.1 | Wheat | cytosol | 87.35 | 89.03 |
HORVU4Hr1G007450.5 | Barley | cytosol | 87.35 | 88.98 |
TraesCS4A01G268300.1 | Wheat | cytosol | 87.24 | 88.97 |
GSMUA_Achr1P22400_001 | Banana | cytosol | 71.17 | 70.74 |
CDY29353 | Canola | cytosol | 64.34 | 64.63 |
CDY64591 | Canola | cytosol | 64.13 | 64.43 |
CDY48864 | Canola | cytosol | 63.88 | 64.3 |
VIT_03s0063g02260.t01 | Wine grape | cytosol | 66.63 | 64.21 |
Bra018885.1-P | Field mustard | cytosol | 55.92 | 63.76 |
CDY22817 | Canola | cytosol | 55.82 | 63.38 |
Bra032281.1-P | Field mustard | cytosol | 64.54 | 63.0 |
AT1G49340.2 | Thale cress | cytosol | 64.69 | 62.52 |
Bra018786.1-P | Field mustard | cytosol | 64.34 | 62.18 |
KRH60863 | Soybean | cytosol | 63.83 | 61.47 |
KRH51547 | Soybean | nucleus | 58.78 | 59.84 |
CDY25197 | Canola | cytosol | 56.73 | 59.72 |
CDY48094 | Canola | cytosol | 38.11 | 55.46 |
Solyc11g043130.1.1 | Tomato | nucleus | 37.81 | 50.48 |
Zm00001d050219_P004 | Maize | cytosol, plasma membrane | 7.4 | 17.49 |
Zm00001d004249_P001 | Maize | mitochondrion | 1.17 | 12.11 |
Zm00001d049076_P001 | Maize | cytosol | 7.76 | 12.0 |
Zm00001d030412_P001 | Maize | cytosol | 1.73 | 5.84 |
Zm00001d007520_P001 | Maize | cytosol, endoplasmic reticulum, extracellular, golgi, mitochondrion, nucleus, plasma membrane, plastid, vacuole | 1.68 | 5.63 |
Protein Annotations
Gene3D:1.10.1070.11 | Gene3D:1.25.40.70 | EntrezGene:103625953 | MapMan:27.5.2.1 | Gene3D:3.30.1010.10 | UniProt:A0A1D6GJH3 |
ProteinID:AQK63499.1 | ProteinID:AQK63523.1 | ProteinID:AQK63538.1 | InterPro:ARM-type_fold | GO:GO:0003674 | GO:GO:0003824 |
GO:GO:0004430 | GO:GO:0005488 | GO:GO:0005515 | GO:GO:0005546 | GO:GO:0005575 | GO:GO:0005622 |
GO:GO:0005623 | GO:GO:0005737 | GO:GO:0005886 | GO:GO:0006464 | GO:GO:0006468 | GO:GO:0006629 |
GO:GO:0007154 | GO:GO:0007165 | GO:GO:0008150 | GO:GO:0008152 | GO:GO:0008289 | GO:GO:0009506 |
GO:GO:0009987 | GO:GO:0016020 | GO:GO:0016301 | GO:GO:0016310 | GO:GO:0016740 | GO:GO:0019538 |
GO:GO:0046854 | GO:GO:0048015 | GO:GO:0048471 | GO:GO:0051015 | GO:GO:0070273 | GO:GO:0070300 |
InterPro:IPR000403 | InterPro:IPR001263 | InterPro:IPR036940 | InterPro:Kinase-like_dom_sf | PFAM:PF00454 | PFAM:PF00613 |
InterPro:PI3/4_kinase_CS | InterPro:PI3/4_kinase_cat_dom | InterPro:PI3/4_kinase_cat_sf | InterPro:PI_Kinase | InterPro:PInositide-3_kin_accessory_dom | ScanProsite:PS00915 |
PFscan:PS50290 | PFscan:PS51545 | PANTHER:PTHR10048 | PANTHER:PTHR10048:SF15 | SMART:SM00145 | SMART:SM00146 |
SUPFAM:SSF48371 | SUPFAM:SSF56112 | UniParc:UPI0008443F30 | EnsemblPlantsGene:Zm00001d013442 | EnsemblPlants:Zm00001d013442_P061 | EnsemblPlants:Zm00001d013442_T061 |
SEG:seg | : | : | : | : | : |
Description
Phosphatidylinositol 4-kinase alpha 1
Coordinates
chr5:+:11681849..11709122
Molecular Weight (calculated)
215232.0 Da
IEP (calculated)
6.195
GRAVY (calculated)
0.003
Length
1960 amino acids
Sequence
(BLAST)
(BLAST)
0001: MEALNELCDL VAAHPDLLLA DKLAWLSSRC AAAPGAAAPQ RASRAHLHSL LALARLLPAG GAGAGAEGDA APPAPLLFFL ASHAFLSPAF WPQSFAPAPF
0101: LARLLPLLAA APSSPALSSA LSAALLAALD VADPTSAPLA RAFLSAAAAA APLPLLPADA APIAARLLLE FLGSEEEPIR AKGKGEDTLG EENGGAKEVV
0201: RRFEEEEVDE LERKEVAFRL TVHMLGGEGG LETEQIGKVR NAAARQVRSL TDFLKIRKRD WREQGTQLRA RINTKLMCCQ AAVGVLVRSV STMDTDIKSS
0301: KDMLQQTLAW FIEATKSCIL SSWRKLKICE ELFCTLLNGI SQITVSRGGQ LLPVLLIPLK PLVVSTCSQA DMAGSSPGAL FEAVVKLSCE IIEFGWTKDR
0401: ALVDTFVMRL AAYVRERNDY EEEDGKGKEA VPVIRLNVIR LLAELCVCLK KWEVVDMILP LFIEHLEEGD ASSPSSLRLR LLDAISRVAC LGFEKSYRES
0501: IVLMTRSYLD KVKALGASEN NTVPSEATTE RTETLPAGFL LVASNLTSTK LRSDYRHRLL SLCSDVGLVA ESKSGRSGAD LMGPLLPAVA DICSDFDPVS
0601: TVEPSLLKLF RNLWFYVVLF GLAPPIQNNE APTKPVSTSL GTAESSSAIA LQAVAGPYMW NSQWSVAVQR IAQGTPPLVV SSVKWLEDEL ELNTLHNPGS
0701: RGGNGNENSA VGQRAALSAA LGGRVEVAAM NTISGVKATY LLAVAFLEIL RFSCNGGILS ATSTLNRSNS AFSCVFEYLL TPNLTPAVTQ CLTAVAHRAF
0801: ETVLSWLDDR ISDIGEGADV RESVLSVHAC FLIKSMSQRD EHVRDVSVKL LTQLKEKFPQ VLWNSSCLDL LLISVHNELT SGPVSDPAWV ATVRSLYQKI
0901: AREWLTSALS YAPCTTQGLI QMVTVLSMLR LPIACLVEVK LAYHENFCKP SGVQRTQHTA DVVSLLSEIR ICSGKNDWNG IRTANVPAVM DSAAAASGAK
1001: KEAPDFTLEV LSTAVVSATV KCNHAGEIAG MRRLFSTMGG INMGMSPPGT QSLHPHQSFD EVFVSKFVSL LQNFVVAAEK QPIDNSQFRE TCSQATALLL
1101: DHMVSDSRAN LEGFSQLIRL LCWCPAYIST PDAMETGIYI WTWLVSAAPS LGPLVLAELV DAWLWTIDTK RGLFASDMNY CGPDAKLRPH LIAGEPEAPP
1201: EKDPVEAIIA HRLWLGFFID RFEVVRHDSI EQLLLLGRML QGTMKSPAHF SHHPAATGTF FTAMLLGLKF CSCQSQSNLQ KCNMGLQLLE DRVYRAALGW
1301: FSYAPEWYES PNKTYAQREA QSVSVFVHFL QNERTSGPVD SVSKLQGREG EPSMADHIHP VWGCVDNYTN AREKRKQLLL TLSQNEADRL EVWAQPIHTK
1401: DTTTFRGKIS SDKWIDHVRT AFAVDPRIAL SMPLRFPTNA TMQSEITQLV QTRLLELRTI PEALPFFITP KAVDENSVLL QQLPHWAPCS VTQALEFLTP
1501: PYKGHPRVMA YVLRVLETYP PETVTFFMPQ LVQSLRYDEG KLVEGYLLGA TRRSNIFAHI LIWHLQGEYV DESEKDAAAL KGSAFQSLLP AVKDKIIESF
1601: TPEARDMFER EFDFFDKVTS ISGVLFPLPK DERRAGIRRE LEKISIPGDD LYLPTATNKL VRGIQLDSGI PLQSAAKVPI MITFNVVDRD GDPNDVKPQA
1701: CIFKVGDDCR QDVLALQVIA LLRDVFQAVG LNLYLFPYGV LPTGPGRGII EVVPDTRSRN QMGETTDGGL LEIFQQDYGP VGSPSFETAR EMFMISSAGY
1801: AVASLLLQPK DRHNGNLLFD SHGRLVHIDF GFILEISPGG NMGFESAHFK LSHEMTQLLD PSGTMKSDTW NQFLRLCVKG YLAARRHMNG ILSTVNLMVD
1901: SGLPCFSRGD PINNLRKRFH PEMNEREAAN FMVRTCADAY NKWTTAGYDL IQYLQQGIEK
0101: LARLLPLLAA APSSPALSSA LSAALLAALD VADPTSAPLA RAFLSAAAAA APLPLLPADA APIAARLLLE FLGSEEEPIR AKGKGEDTLG EENGGAKEVV
0201: RRFEEEEVDE LERKEVAFRL TVHMLGGEGG LETEQIGKVR NAAARQVRSL TDFLKIRKRD WREQGTQLRA RINTKLMCCQ AAVGVLVRSV STMDTDIKSS
0301: KDMLQQTLAW FIEATKSCIL SSWRKLKICE ELFCTLLNGI SQITVSRGGQ LLPVLLIPLK PLVVSTCSQA DMAGSSPGAL FEAVVKLSCE IIEFGWTKDR
0401: ALVDTFVMRL AAYVRERNDY EEEDGKGKEA VPVIRLNVIR LLAELCVCLK KWEVVDMILP LFIEHLEEGD ASSPSSLRLR LLDAISRVAC LGFEKSYRES
0501: IVLMTRSYLD KVKALGASEN NTVPSEATTE RTETLPAGFL LVASNLTSTK LRSDYRHRLL SLCSDVGLVA ESKSGRSGAD LMGPLLPAVA DICSDFDPVS
0601: TVEPSLLKLF RNLWFYVVLF GLAPPIQNNE APTKPVSTSL GTAESSSAIA LQAVAGPYMW NSQWSVAVQR IAQGTPPLVV SSVKWLEDEL ELNTLHNPGS
0701: RGGNGNENSA VGQRAALSAA LGGRVEVAAM NTISGVKATY LLAVAFLEIL RFSCNGGILS ATSTLNRSNS AFSCVFEYLL TPNLTPAVTQ CLTAVAHRAF
0801: ETVLSWLDDR ISDIGEGADV RESVLSVHAC FLIKSMSQRD EHVRDVSVKL LTQLKEKFPQ VLWNSSCLDL LLISVHNELT SGPVSDPAWV ATVRSLYQKI
0901: AREWLTSALS YAPCTTQGLI QMVTVLSMLR LPIACLVEVK LAYHENFCKP SGVQRTQHTA DVVSLLSEIR ICSGKNDWNG IRTANVPAVM DSAAAASGAK
1001: KEAPDFTLEV LSTAVVSATV KCNHAGEIAG MRRLFSTMGG INMGMSPPGT QSLHPHQSFD EVFVSKFVSL LQNFVVAAEK QPIDNSQFRE TCSQATALLL
1101: DHMVSDSRAN LEGFSQLIRL LCWCPAYIST PDAMETGIYI WTWLVSAAPS LGPLVLAELV DAWLWTIDTK RGLFASDMNY CGPDAKLRPH LIAGEPEAPP
1201: EKDPVEAIIA HRLWLGFFID RFEVVRHDSI EQLLLLGRML QGTMKSPAHF SHHPAATGTF FTAMLLGLKF CSCQSQSNLQ KCNMGLQLLE DRVYRAALGW
1301: FSYAPEWYES PNKTYAQREA QSVSVFVHFL QNERTSGPVD SVSKLQGREG EPSMADHIHP VWGCVDNYTN AREKRKQLLL TLSQNEADRL EVWAQPIHTK
1401: DTTTFRGKIS SDKWIDHVRT AFAVDPRIAL SMPLRFPTNA TMQSEITQLV QTRLLELRTI PEALPFFITP KAVDENSVLL QQLPHWAPCS VTQALEFLTP
1501: PYKGHPRVMA YVLRVLETYP PETVTFFMPQ LVQSLRYDEG KLVEGYLLGA TRRSNIFAHI LIWHLQGEYV DESEKDAAAL KGSAFQSLLP AVKDKIIESF
1601: TPEARDMFER EFDFFDKVTS ISGVLFPLPK DERRAGIRRE LEKISIPGDD LYLPTATNKL VRGIQLDSGI PLQSAAKVPI MITFNVVDRD GDPNDVKPQA
1701: CIFKVGDDCR QDVLALQVIA LLRDVFQAVG LNLYLFPYGV LPTGPGRGII EVVPDTRSRN QMGETTDGGL LEIFQQDYGP VGSPSFETAR EMFMISSAGY
1801: AVASLLLQPK DRHNGNLLFD SHGRLVHIDF GFILEISPGG NMGFESAHFK LSHEMTQLLD PSGTMKSDTW NQFLRLCVKG YLAARRHMNG ILSTVNLMVD
1901: SGLPCFSRGD PINNLRKRFH PEMNEREAAN FMVRTCADAY NKWTTAGYDL IQYLQQGIEK
0001: MEALTELCDI IAKNPKQFSE KLAWICGRCP QTEWLLAESP RVSRSHLNAV LAVARIISKN PESIDNRAKS VVNEFLSAIP ASFRRSFWPH SFPSQLISSF
0101: YCDFLSYLSC AADLSPEFGT EVARFTGEVV IAAIAPSSGD SDGDPAISKA FLVALSQHFP SILQSDGDKL ITMLLDQFVL NRAPASPKEQ RQQNSANSET
0201: DTSSSQGSPI STNRYPSGKT EMASPGDEVA SHGSNLSSKS SSSVVMNGGS IVWKSGVDQL SFGFSEGSGG ANPVFRQQVA SFEDESIESL EKQEIAFRLI
0301: THILDKVKID SKLQDQVRFI AKRQLQSMSA FLKSRKRDWN EQGQVLKTRV NAKLSVYQAA AKMKIKSLVS LETDGKTSKR LVLETLALLL DAADACLTSV
0401: WRKMKACEEL FDSLLSGIAK IAVARGGQPL RVLLIRLKPL VLAVCALPDQ GAMLESIFKT SCVIIESAWA KDRAPVDNFI MGLASSIRER NDYEEQVDRE
0501: KQVPAVQLNV IRLLADLNVA VKKPEVADMI LPLFIESLEE GDASTPSFLR LQLLDAVSRI ATLGFDKSYR ETVVLMTRSY LSKLSSVGSV ESKTSAPEAT
0601: TERVETLPAG FLTIASGLMD TKLRSDYRHR LLSLCSDVGL AAESKSGGSG VDFLGPLLPA VAEICSDFDP TMDVEPSLLK LFRNLWFYIA LFGLAPPIVK
0701: TPTPPLKSTS NSVNSVGSMS ATALQAVGGP YMWDNQWALA VQRIAQGTPP LVVSSVKWLE DELELNALHN PGSRRGNGNE KVASTQRLAL STALGGRVDV
0801: AAMNTISGVK ATYLLAVAFL EIIRFISNGG ILNGESSVSA SRSAFSCVFE YLKTPNLTPA VSQCLTAIVH RAFETAVSWL EDRISLTGKD ARNRELTTYA
0901: HACFLIKSMS QRDEHVRDIS VNLLTQLRDK FPQVLWHSSC LDSLLFSVHD NTPSTVVNDP AWTAAVRSLY QKVVREWIII SLSYAPCTSQ GLLQDKLCKA
1001: NTWQRAQTTT DVVSLLSEIK IGTGKNELWS GIRTANIPAV MAAAAAASGA NLKVSEAFNL EVLGTGVVSA TVKCNHAGEI AGMRRLYNSI GGFQSGSTPS
1101: GFGGGLQRLI SGAFSQAPQP EDDSFNEMLI ARFVRLLQQF VNTAEKGGEV EKSQFRETCS QATALLLSNL GGESKTNVEG FSQLLRLLCW CPAYISTPDA
1201: METGIFIWTW LVSAAPQLVS LVLAELVDAW IWTIDTKRGL FASDVRYSGP AAKLRPHLSP GEPEDPPESD PVDQIVAHRL WLGFLIDRFE VVRHNSAEQL
1301: LLLGRMLQRS TDLEWCFTRH PAAAGTFFSL MLLGLKFCSC QTQGNMQKFR SGLQLLEDRI YRTSLGWFAH QPEWYDVNIP NFCHSEALSV SVFVHFLSNE
1401: LSESSQSDSK GKPRESGNLI DVTDQYHPVW GEMDNYTLGK EKRKQLLLML CQHEADRLDV WAQPISSKDS PYSRLKISSE KWTEYAKTAF SVDPRIALSV
1501: ASRFPANASV KSEVTQLVQT NIVDLRTIPE ALPYFVTPKN VEENSVLLQQ LPHWAACSIT QALEFLTPAY KGHPRVMAYV LRVLESYPPE RVTFFMPQLV
1601: QSLRYDDGRL VEGYLLRATQ RSDIFAHILI WHLQGEDVQE TPKDGSIDKN AAFQEILPQV RQHIIDGFSP NALDMFTREF DFFDKVTSIS GVLFPLPKEE
1701: RRAGIRRELE KIEMQGDDLY LPTAPNKLVR GIRVDSGIPL QSAAKVPIMI TFNVIDRDGD HSDVKPQACI FKVGDDCRQD VLALQVISLL RDIFQAAGLN
1801: LYLFPYGVLP TGAERGIIEV VPNTRSRSQM GETTDGGLYE IFQQDYGPVG STTFETAREN FLISSAGYAV ASLLLQPKDR HNGNLLFDDV GRLVHIDFGF
1901: ILETSPGGNM RFESAHFKLS HEMTQLLDPS GVMKSKTWHQ FVSLCVKGYL AARRQMDGII STVQMMLESG LPCFSRGDPI GNLRKRFHPE MSEREAAHFM
2001: IHVCTDAYNK WTTAGYDLIQ YLQQGIEK
0101: YCDFLSYLSC AADLSPEFGT EVARFTGEVV IAAIAPSSGD SDGDPAISKA FLVALSQHFP SILQSDGDKL ITMLLDQFVL NRAPASPKEQ RQQNSANSET
0201: DTSSSQGSPI STNRYPSGKT EMASPGDEVA SHGSNLSSKS SSSVVMNGGS IVWKSGVDQL SFGFSEGSGG ANPVFRQQVA SFEDESIESL EKQEIAFRLI
0301: THILDKVKID SKLQDQVRFI AKRQLQSMSA FLKSRKRDWN EQGQVLKTRV NAKLSVYQAA AKMKIKSLVS LETDGKTSKR LVLETLALLL DAADACLTSV
0401: WRKMKACEEL FDSLLSGIAK IAVARGGQPL RVLLIRLKPL VLAVCALPDQ GAMLESIFKT SCVIIESAWA KDRAPVDNFI MGLASSIRER NDYEEQVDRE
0501: KQVPAVQLNV IRLLADLNVA VKKPEVADMI LPLFIESLEE GDASTPSFLR LQLLDAVSRI ATLGFDKSYR ETVVLMTRSY LSKLSSVGSV ESKTSAPEAT
0601: TERVETLPAG FLTIASGLMD TKLRSDYRHR LLSLCSDVGL AAESKSGGSG VDFLGPLLPA VAEICSDFDP TMDVEPSLLK LFRNLWFYIA LFGLAPPIVK
0701: TPTPPLKSTS NSVNSVGSMS ATALQAVGGP YMWDNQWALA VQRIAQGTPP LVVSSVKWLE DELELNALHN PGSRRGNGNE KVASTQRLAL STALGGRVDV
0801: AAMNTISGVK ATYLLAVAFL EIIRFISNGG ILNGESSVSA SRSAFSCVFE YLKTPNLTPA VSQCLTAIVH RAFETAVSWL EDRISLTGKD ARNRELTTYA
0901: HACFLIKSMS QRDEHVRDIS VNLLTQLRDK FPQVLWHSSC LDSLLFSVHD NTPSTVVNDP AWTAAVRSLY QKVVREWIII SLSYAPCTSQ GLLQDKLCKA
1001: NTWQRAQTTT DVVSLLSEIK IGTGKNELWS GIRTANIPAV MAAAAAASGA NLKVSEAFNL EVLGTGVVSA TVKCNHAGEI AGMRRLYNSI GGFQSGSTPS
1101: GFGGGLQRLI SGAFSQAPQP EDDSFNEMLI ARFVRLLQQF VNTAEKGGEV EKSQFRETCS QATALLLSNL GGESKTNVEG FSQLLRLLCW CPAYISTPDA
1201: METGIFIWTW LVSAAPQLVS LVLAELVDAW IWTIDTKRGL FASDVRYSGP AAKLRPHLSP GEPEDPPESD PVDQIVAHRL WLGFLIDRFE VVRHNSAEQL
1301: LLLGRMLQRS TDLEWCFTRH PAAAGTFFSL MLLGLKFCSC QTQGNMQKFR SGLQLLEDRI YRTSLGWFAH QPEWYDVNIP NFCHSEALSV SVFVHFLSNE
1401: LSESSQSDSK GKPRESGNLI DVTDQYHPVW GEMDNYTLGK EKRKQLLLML CQHEADRLDV WAQPISSKDS PYSRLKISSE KWTEYAKTAF SVDPRIALSV
1501: ASRFPANASV KSEVTQLVQT NIVDLRTIPE ALPYFVTPKN VEENSVLLQQ LPHWAACSIT QALEFLTPAY KGHPRVMAYV LRVLESYPPE RVTFFMPQLV
1601: QSLRYDDGRL VEGYLLRATQ RSDIFAHILI WHLQGEDVQE TPKDGSIDKN AAFQEILPQV RQHIIDGFSP NALDMFTREF DFFDKVTSIS GVLFPLPKEE
1701: RRAGIRRELE KIEMQGDDLY LPTAPNKLVR GIRVDSGIPL QSAAKVPIMI TFNVIDRDGD HSDVKPQACI FKVGDDCRQD VLALQVISLL RDIFQAAGLN
1801: LYLFPYGVLP TGAERGIIEV VPNTRSRSQM GETTDGGLYE IFQQDYGPVG STTFETAREN FLISSAGYAV ASLLLQPKDR HNGNLLFDDV GRLVHIDFGF
1901: ILETSPGGNM RFESAHFKLS HEMTQLLDPS GVMKSKTWHQ FVSLCVKGYL AARRQMDGII STVQMMLESG LPCFSRGDPI GNLRKRFHPE MSEREAAHFM
2001: IHVCTDAYNK WTTAGYDLIQ YLQQGIEK
Arabidopsis Description
PI4KA1Phosphatidylinositol 4-kinase alpha 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9SXA1]
SUBAcon: [cytosol]
SUBAcon: [cytosol]
Hydropathy Plot
About CropPAL
The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.