CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus, cytosol

Predictor Summary:

cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, nucleus
Any Predictor:	cytosol, mitochondrion
BaCelLo:	cytosol
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH25286	Soybean	cytosol	98.0	98.0
VIT_06s0080g00940.t01	Wine grape	cytosol	87.71	88.47
PGSC0003DMT400067695	Potato	cytoskeleton, cytosol, peroxisome	88.29	88.03
Solyc11g068730.1.1	Tomato	extracellular, nucleus	87.71	87.46
CDY09130	Canola	cytosol	77.71	76.19
CDY35679	Canola	cytosol	77.43	76.12
Bra020184.1-P	Field mustard	cytosol	77.14	75.63
AT5G22300.1	Thale cress	cytosol, peroxisome, plastid	75.71	74.65
TraesCS6A01G340200.1	Wheat	cytosol	20.57	39.56

Protein Annotations

EntrezGene:100778110	Gene3D:3.60.110.10	MapMan:9.5.2.5	UniProt:A0A0R0HW00	EMBL:ACUP02007004	InterPro:C-N_Hydrolase
InterPro:C-N_Hydrolase_sf	EnsemblPlantsGene:GLYMA_11G180900	GO:GO:0003674	GO:GO:0003824	GO:GO:0006807	GO:GO:0008150
GO:GO:0008152	GO:GO:0016787	GO:GO:0016810	InterPro:IPR003010	InterPro:IPR036526	EnsemblPlants:KRH30383
ProteinID:KRH30383	ProteinID:KRH30383.1	InterPro:Nitrilase/CN_hydratase_CS	PFAM:PF00795	ScanProsite:PS00920	ScanProsite:PS00921
PFscan:PS50263	PANTHER:PTHR23088	PANTHER:PTHR23088:SF31	SUPFAM:SSF56317	UniParc:UPI000233D848	:

Description

hypothetical protein

Coordinates

chr11:+:24764500..24768959

Molecular Weight (calculated)

37897.2 Da

IEP (calculated)

5.089

GRAVY (calculated)

-0.039

Length

350 amino acids

Sequence
(BLAST)

001: MALVTTNPTI NEGPLFAEVD MGSDFNAPTV RATVVQASTI FYDTPATLDK AERLLAEAAS YGSQLVVFPE AFVGGYPRGS AFGLSIGNRT AKGREEFRKY
101: HSAAIDVPGP EVDRLAAMAG KYKVHLVMGV IERDGYTLYC TVLFFDSQGH YLGKHRKIMP TALERVIWGF GDGSTIPVFE TPVGKIGAAI CWENRMPLLR
201: TAMYAKGVEI YCAPTADSRD VWQASMTHIA LEGGCFVLSA NQFCRRKDYP PPPEYVFAGT EEDLTPDSVV CAGGSVIISP SGAVLAGPNY DGEALISADL
301: DLGEIARAKF DFDVVGHYSR PEVLSLIVKD HPTNPVTFTS TSTKIEDKTK

Best Arabidopsis Sequence Match ( AT5G22300.1 )
(BLAST)

001: MSMQQETSHM TAAPQTNGHQ IFPEIDMSAG DSSSIVRATV VQASTVFYDT PATLDKAERL LSEAAENGSQ LVVFPEAFIG GYPRGSTFEL AIGSRTAKGR
101: DDFRKYHASA IDVPGPEVER LALMAKKYKV YLVMGVIERE GYTLYCTVLF FDSQGLFLGK HRKLMPTALE RCIWGFGDGS TIPVFDTPIG KIGAAICWEN
201: RMPSLRTAMY AKGIEIYCAP TADSRETWLA SMTHIALEGG CFVLSANQFC RRKDYPSPPE YMFSGSEESL TPDSVVCAGG SSIISPLGIV LAGPNYRGEA
301: LITADLDLGD IARAKFDFDV VGHYSRPEVF SLNIREHPRK AVSFKTSKVM EDESV

Arabidopsis Description

NIT4Bifunctional nitrilase/nitrile hydratase NIT4 [Source:UniProtKB/Swiss-Prot;Acc:P46011]
SUBAcon: [peroxisome,plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.