Factsheet for Bra020184.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
plastid 1
plasma membrane 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY09130	Canola	cytosol	99.16	99.16
AT5G22300.1	Thale cress	cytosol, peroxisome, plastid	85.15	85.63
PGSC0003DMT400067695	Potato	cytoskeleton, cytosol, peroxisome	76.75	78.06
Solyc11g068730.1.1	Tomato	extracellular, nucleus	76.47	77.78
KRH30383	Soybean	cytosol, nucleus	75.63	77.14
KRH25286	Soybean	cytosol	75.07	76.57
GSMUA_Achr8P13970_001	Banana	plastid	71.71	73.78
TraesCS6B01G260800.2	Wheat	cytosol	67.51	70.67
HORVU6Hr1G059740.1	Barley	cytosol	67.23	70.38
TraesCS6D01G215300.1	Wheat	cytosol	66.95	70.09
Zm00001d051214_P001	Maize	cytosol	70.31	69.53
EES05474	Sorghum	cytosol	69.75	68.98
Os02t0635200-01	Rice	plasma membrane	69.75	68.78
EES05473	Sorghum	cytosol	66.11	67.82
VIT_02s0033g00790.t01	Wine grape	cytosol, peroxisome, plastid	62.47	67.78
VIT_02s0109g00430.t01	Wine grape	cytosol, peroxisome, plastid	62.18	67.68
TraesCS6D01G215400.2	Wheat	cytosol	68.91	67.58
TraesCS6A01G232500.1	Wheat	cytosol	68.63	67.31
TraesCS6B01G261000.1	Wheat	cytosol	67.51	66.94
KXG30695	Sorghum	cytosol	68.07	66.94
VIT_02s0033g00670.t01	Wine grape	cytosol, peroxisome, plastid	61.62	66.87
VIT_02s0033g00850.t01	Wine grape	cytosol, peroxisome, plastid	54.06	66.78
TraesCS6B01G260700.2	Wheat	cytosol	68.07	66.76
TraesCS6A01G232300.1	Wheat	cytosol	67.23	66.67
VIT_02s0033g00770.t01	Wine grape	cytosol, peroxisome, plastid	61.34	66.57
Os02t0635000-01	Rice	cytosol	66.39	66.39
VIT_02s0033g00800.t01	Wine grape	cytosol, peroxisome, plastid	61.06	66.26
VIT_02s0033g00870.t01	Wine grape	cytosol, peroxisome, plastid	61.06	66.26
VIT_02s0033g00660.t01	Wine grape	cytosol, peroxisome, plastid	61.06	66.26
VIT_02s0033g00700.t01	Wine grape	cytosol, peroxisome, plastid	61.9	66.17
Bra028932.1-P	Field mustard	cytosol	61.62	64.14
TraesCS6A01G232400.2	Wheat	cytosol	60.22	62.5
HORVU6Hr1G059760.1	Barley	cytosol	58.26	62.28
Zm00001d017352_P001	Maize	cytosol	67.79	60.65
Bra021681.1-P	Field mustard	cytosol	59.1	60.29
Bra023598.1-P	Field mustard	cytosol	57.7	59.88
EES12535	Sorghum	cytosol	56.3	58.77
Bra035006.1-P	Field mustard	cytosol	52.38	56.67
VIT_02s0033g00630.t01	Wine grape	cytosol	54.62	56.2
VIT_02s0033g00840.t01	Wine grape	cytosol, extracellular	19.05	54.4
HORVU6Hr1G059730.3	Barley	plastid	67.23	53.45
TraesCS6A01G340200.1	Wheat	cytosol	16.53	32.42

Protein Annotations

Gene3D:3.60.110.10	MapMan:9.5.2.5	EMBL:AB254042	ProteinID:BAG72076	ProteinID:BAG72076.1	EnsemblPlantsGene:Bra020184
EnsemblPlants:Bra020184.1	EnsemblPlants:Bra020184.1-P	InterPro:C-N_Hydrolase	InterPro:C-N_Hydrolase_sf	GO:GO:0000257	GO:GO:0003674
GO:GO:0003824	GO:GO:0006807	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016787
GO:GO:0016810	GO:GO:0018822	GO:GO:0019499	GO:GO:0047427	GO:GO:0047558	GO:GO:0051410
GO:GO:0080061	InterPro:IPR003010	InterPro:IPR036526	UniProt:M4DUJ1	InterPro:Nitrilase/CN_hydratase_CS	PFAM:PF00795
ScanProsite:PS00921	PFscan:PS50263	PANTHER:PTHR23088	PANTHER:PTHR23088:SF31	SUPFAM:SSF56317	UniParc:UPI0002543497

Description

AT5G22300 (E=1e-180) NIT4 | NIT4 (NITRILASE 4); 3-cyanoalanine hydratase/ cyanoalanine nitrilase/ indole-3-acetonitrile nitrilase/ nitrilase/ nitrile hydratase

Coordinates

chrA02:+:5444879..5446649

Molecular Weight (calculated)

38941.5 Da

IEP (calculated)

5.122

GRAVY (calculated)

-0.099

Length

357 amino acids

Sequence
(BLAST)

001: MSTHQQDMSL VTSTPPINNG NQIFPEIEMS GDSSSIVRAT VVQACTIFYD TPATLDKAER LLAEAADNGA QLVVVPEAFI GGYPRGSSFE LAIGARTAKG
101: RDDFRKYLAS AIDVPGPEVE RMAEMARKYK VFLVMGVIER EGYTLYCSVL FFDSHGQFLG KHRKLMPTAL ERCIWGFGDG STIPVFDTPI GKIGAAICWE
201: NRMPSLRTAM YAKGIEIYCA PTADARETWL ASMTHIALEG GCFVLSANQF CRRKDYPPPP EYTFSGSEES LTPDSVVCAG GSSIISPLGI VLAGPNYEGE
301: GLISADLDLG DIARAKFDFD VVGHYSRPEV FSLNIKEHPR KAVSFTSKVT KDETVKN

Best Arabidopsis Sequence Match ( AT5G22300.1 )
(BLAST)

001: MSMQQETSHM TAAPQTNGHQ IFPEIDMSAG DSSSIVRATV VQASTVFYDT PATLDKAERL LSEAAENGSQ LVVFPEAFIG GYPRGSTFEL AIGSRTAKGR
101: DDFRKYHASA IDVPGPEVER LALMAKKYKV YLVMGVIERE GYTLYCTVLF FDSQGLFLGK HRKLMPTALE RCIWGFGDGS TIPVFDTPIG KIGAAICWEN
201: RMPSLRTAMY AKGIEIYCAP TADSRETWLA SMTHIALEGG CFVLSANQFC RRKDYPSPPE YMFSGSEESL TPDSVVCAGG SSIISPLGIV LAGPNYRGEA
301: LITADLDLGD IARAKFDFDV VGHYSRPEVF SLNIREHPRK AVSFKTSKVM EDESV

Arabidopsis Description

NIT4Bifunctional nitrilase/nitrile hydratase NIT4 [Source:UniProtKB/Swiss-Prot;Acc:P46011]
SUBAcon: [peroxisome,plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.