Factsheet for Os02t0635200-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 3
plastid 1
plasma membrane 1
mitochondrion 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES05474	Sorghum	cytosol	91.44	91.69
TraesCS6A01G232300.1	Wheat	cytosol	90.33	90.83
TraesCS6B01G261000.1	Wheat	cytosol	90.33	90.83
TraesCS6A01G232500.1	Wheat	cytosol	91.16	90.66
TraesCS6D01G215400.2	Wheat	cytosol	91.16	90.66
TraesCS6B01G260700.2	Wheat	cytosol	90.88	90.38
Zm00001d051214_P001	Maize	cytosol	89.78	90.03
GSMUA_Achr8P13970_001	Banana	plastid	78.18	81.56
Os02t0635000-01	Rice	cytosol	75.97	77.03
VIT_06s0080g00950.t01	Wine grape	cytosol	24.86	74.38
HORVU6Hr1G059730.3	Barley	plastid	89.23	71.94
CDY35679	Canola	cytosol	69.89	71.07
CDY09130	Canola	cytosol	69.34	70.31
AT5G22300.1	Thale cress	cytosol, peroxisome, plastid	68.78	70.14
Bra020184.1-P	Field mustard	cytosol	68.78	69.75

Protein Annotations

Gene3D:3.60.110.10	EntrezGene:4330077	MapMan:9.5.2.5	EMBL:AB027054	EMBL:AK104033	EMBL:AK121244
ProteinID:BAD25100.1	ProteinID:BAF09432.1	ProteinID:BAS79936.1	InterPro:C-N_Hydrolase	InterPro:C-N_Hydrolase_sf	ProteinID:EAZ23921.1
GO:GO:0000257	GO:GO:0003674	GO:GO:0003824	GO:GO:0006807	GO:GO:0008150	GO:GO:0008152
GO:GO:0016787	GO:GO:0016810	GO:GO:0047427	InterPro:IPR003010	InterPro:IPR036526	InterPro:Nitrilase/CN_hydratase_CS
EnsemblPlantsGene:Os02g0635200	EnsemblPlants:Os02t0635200-01	PFAM:PF00795	ScanProsite:PS00920	ScanProsite:PS00921	PFscan:PS50263
PANTHER:PTHR23088	PANTHER:PTHR23088:SF31	UniProt:Q6H849	SUPFAM:SSF56317	UniParc:UPI00003B4351	RefSeq:XP_015622782.1
SEG:seg	:	:	:	:	:

Description

nitrilase 1, Nitrilase 2Similar to Nitrilase 2 (EC 3.5.5.1). (Os02t0635200-01)

Coordinates

chr2:+:25465734..25469526

Molecular Weight (calculated)

38678.3 Da

IEP (calculated)

4.984

GRAVY (calculated)

-0.009

Length

362 amino acids

Sequence
(BLAST)

001: MAMVPSGSGG GPPVIAEVEM NGGADSGAAT VRATVVQAST VFYDTPATLD KAERLIEEAA GYGSQLVVFP EAFVGGYPRG STFGFGANIS IGNPKDKGKE
101: EFRKYHAAAI EVPGPEVTRL AAMAGKYKVF LVMGVIEREG YTLYCSVLFF DPLGRYLGKH RKLMPTALER IIWGFGDGST IPVYDTPLGK IGALICWENK
201: MPLLRTALYG KGIEIYCAPT ADSRQVWQAS MTHIALEGGC FVLSANQFCR RKDYPPPPEY VFSGLGEEPS PDTVVCPGGS VIISPSGEVL AGPNYEGEAL
301: ITADLDLGEI VRAKFDFDVV GHYARPEVLS LVVNDQPHLP VSFTSAAEKT TAAKSDSTAK PY

Best Arabidopsis Sequence Match ( AT5G22300.1 )
(BLAST)

001: MSMQQETSHM TAAPQTNGHQ IFPEIDMSAG DSSSIVRATV VQASTVFYDT PATLDKAERL LSEAAENGSQ LVVFPEAFIG GYPRGSTFEL AIGSRTAKGR
101: DDFRKYHASA IDVPGPEVER LALMAKKYKV YLVMGVIERE GYTLYCTVLF FDSQGLFLGK HRKLMPTALE RCIWGFGDGS TIPVFDTPIG KIGAAICWEN
201: RMPSLRTAMY AKGIEIYCAP TADSRETWLA SMTHIALEGG CFVLSANQFC RRKDYPSPPE YMFSGSEESL TPDSVVCAGG SSIISPLGIV LAGPNYRGEA
301: LITADLDLGD IARAKFDFDV VGHYSRPEVF SLNIREHPRK AVSFKTSKVM EDESV

Arabidopsis Description

NIT4Bifunctional nitrilase/nitrile hydratase NIT4 [Source:UniProtKB/Swiss-Prot;Acc:P46011]
SUBAcon: [peroxisome,plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.