Factsheet for Bra009294.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 4
nucleus 1
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra002301.1-P
Bra028949.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G07070.1	Bra002301.1-P	AT4G01420.1	11230129
AT5G07070.1	Bra028949.1-P	AT5G55990.1	11230129

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX70005	Canola	cytosol	99.55	99.55
Bra028709.1-P	Field mustard	cytosol	80.23	80.41
AT5G07070.1	Thale cress	cytosol	74.55	71.93
Bra009293.1-P	Field mustard	cytosol	75.45	71.4
Bra020371.1-P	Field mustard	cytosol	70.45	69.98
Bra002654.1-P	Field mustard	cytosol	72.73	69.26
VIT_06s0004g07830.t01	Wine grape	cytosol	63.64	62.5
GSMUA_Achr7P22140_001	Banana	cytosol	50.45	60.16
KRH72808	Soybean	cytosol	63.18	60.04
KRG98172	Soybean	cytosol	61.82	58.87
KRH30131	Soybean	cytosol	61.14	58.23
Solyc06g007430.1.1	Tomato	cytosol	63.18	58.16
PGSC0003DMT400052958	Potato	cytosol	61.59	57.66
Bra021992.1-P	Field mustard	cytosol	56.82	57.47
Os05t0476350-00	Rice	cytosol	56.14	56.78
EES15653	Sorghum	cytosol	57.27	56.63
EES09124	Sorghum	cytosol	56.36	55.98
Zm00001d052340_P001	Maize	cytosol	55.68	55.3
Os12t0113500-01	Rice	cytosol	55.0	55.13
EES06320	Sorghum	cytosol	57.73	55.1
Os11t0113700-01	Rice	cytosol	54.32	55.07
TraesCS4B01G120400.1	Wheat	cytosol, mitochondrion	55.23	54.73
HORVU5Hr1G046880.1	Barley	cytosol	54.09	54.09
TraesCS4A01G194800.1	Wheat	cytosol	55.0	53.9
TraesCS4D01G118500.2	Wheat	cytosol	55.0	53.9
TraesCS5A01G148000.1	Wheat	cytosol	53.64	53.88
TraesCS5B01G146500.1	Wheat	cytosol	52.95	53.81
TraesCS5D01G144800.1	Wheat	cytosol	53.41	53.65
HORVU4Hr1G022630.1	Barley	cytosol, mitochondrion	54.09	53.6
HORVU0Hr1G015380.1	Barley	cytosol	56.36	53.33
HORVU5Hr1G046830.3	Barley	cytosol	54.09	53.24
EES19682	Sorghum	cytosol	54.77	52.85
Bra010263.1-P	Field mustard	cytosol	51.82	52.78
Bra011236.1-P	Field mustard	cytosol	52.5	52.38
Bra024032.1-P	Field mustard	cytosol	51.82	52.17
Zm00001d015325_P001	Maize	cytosol	55.68	52.13
TraesCS6A01G135100.1	Wheat	cytosol	56.36	51.56
Zm00001d052544_P001	Maize	cytosol	50.0	51.52
Bra025051.1-P	Field mustard	cytosol	41.14	51.42
Zm00001d023365_P001	Maize	cytosol	50.23	51.16
TraesCS6D01G124200.1	Wheat	cytosol	56.36	51.03
TraesCS1B01G309400.1	Wheat	cytosol	54.32	50.64
TraesCS1D01G298500.1	Wheat	cytosol	54.32	50.64
TraesCS6B01G163100.1	Wheat	cytosol	56.36	50.51
Os02t0161000-01	Rice	cytosol	56.59	50.51
TraesCS3A01G346100.1	Wheat	cytosol	58.18	50.49
TraesCS3D01G339800.1	Wheat	cytosol	58.18	50.49
Zm00001d023353_P001	Maize	cytosol	49.09	50.35
Bra009003.1-P	Field mustard	cytosol	49.55	50.23
TraesCS1A01G299600.1	Wheat	cytosol	53.86	50.21
Os01t0824600-01	Rice	cytosol	57.27	50.2
KXG33627	Sorghum	cytosol	58.86	50.19
Zm00001d010459_P001	Maize	cytosol	51.14	50.0
Zm00001d023360_P001	Maize	cytosol	48.86	50.0
Zm00001d008833_P001	Maize	cytosol	49.09	50.0
TraesCS3B01G377800.1	Wheat	cytosol	57.5	49.9
HORVU3Hr1G081420.1	Barley	cytosol	57.5	49.71
Zm00001d023631_P001	Maize	cytosol	50.23	49.11
HORVU1Hr1G070100.9	Barley	cytosol	53.64	47.48
Bra032042.1-P	Field mustard	cytosol	48.86	46.64
Bra027311.1-P	Field mustard	cytosol	38.64	46.32
Bra022794.1-P	Field mustard	cytosol	44.55	45.9
Zm00001d007101_P001	Maize	cytosol	32.05	45.34
Bra013340.1-P	Field mustard	cytosol	50.68	45.14
Bra025052.1-P	Field mustard	cytosol	49.09	44.91
Bra020996.1-P	Field mustard	cytosol	49.77	44.88
Bra018319.1-P	Field mustard	cytosol	45.0	44.8
Bra021628.1-P	Field mustard	cytosol	38.64	44.39
Bra005447.1-P	Field mustard	cytosol, mitochondrion, plastid	48.64	43.5
Bra028912.1-P	Field mustard	cytosol	35.91	42.82
Bra005446.1-P	Field mustard	mitochondrion	47.27	42.8
Bra005103.1-P	Field mustard	cytosol	41.36	42.62
Bra010841.1-P	Field mustard	cytosol, plastid	50.23	42.18
Bra000066.1-P	Field mustard	cytosol	40.45	41.4
Bra021897.1-P	Field mustard	plastid	47.95	41.21
Zm00001d043038_P001	Maize	cytosol, mitochondrion, peroxisome, plasma membrane	57.73	25.53

Protein Annotations

KEGG:04150+2.7.11.1	KEGG:04151+2.7.11.1	KEGG:04714+2.7.11.1	KEGG:04926+2.7.11.1	KEGG:05165+2.7.11.1	Gene3D:1.10.510.10
MapMan:18.4.5.3	Gene3D:3.30.310.80	EnsemblPlantsGene:Bra009294	EnsemblPlants:Bra009294.1	EnsemblPlants:Bra009294.1-P	InterPro:Ca/CaM-dep_Ca-dep_prot_Kinase
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0006464	GO:GO:0006468	GO:GO:0007154	GO:GO:0007165
GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016020	GO:GO:0016021	GO:GO:0016301
GO:GO:0016310	GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:IPR018451	InterPro:Kinase-like_dom_sf
UniProt:M4CYJ7	InterPro:NAF/FISL_domain	InterPro:NAF_dom	PFAM:PF00069	PFAM:PF03822	PIRSF:PIRSF000654
ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PFscan:PS50816	PANTHER:PTHR43895	PANTHER:PTHR43895:SF16
InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	TMHMM:TMhelix
UniParc:UPI0002543980	SEG:seg	:	:	:	:

Description

AT5G45820 (E=4e-136) CIPK20, SnRK3.6, PKS18 | CIPK20 (CBL-INTERACTING PROTEIN KINASE 20); kinase/ protein serine/threonine kinase

Coordinates

chrA10:+:16028783..16030105

Molecular Weight (calculated)

49407.1 Da

IEP (calculated)

9.299

GRAVY (calculated)

-0.378

Length

440 amino acids

Sequence
(BLAST)

001: MENKPSILTD RYEVGRLLGQ GNFAKVFYGR SVHTNESVAI KMIDKDKLKK VGHSEQIKRE ISVMSLAKHP NVVNLHEVMA TKTKIYLVLE YCKGGELFKK
101: MVKGKLAENV AWKYFHQLID AVDFCHSRGV YHRDIKPENL LLDEHDNLKV SDFGLSALAE SKRADGLLHT ACGSHAYACP EIVNRKGYDG TKADVWSCGV
201: VLFVLLTGYL PFYASNLMDM YRKIGKAQFK CPREFPPEAK RLLSKMLDPN PESRITISKI KESSWFKKGL PMKQKRIEKE AVGGSGSGSG PSEPPPQLSS
301: MNAFDIIALS SGLALGGLFG EVYSKKESKF TSRKPASEII CKLEEVAKGL KMKIRKQEAG LFKMEGGKEG RKGRLLIDAE IFEVTETFHL VEVKKCSGDT
401: MEYQRMVEED LRPALADIVW VWQGENEHVL RGLKEGQEPL

Best Arabidopsis Sequence Match ( AT5G07070.1 )
(BLAST)

001: MENKPSVLTE RYEVGRLLGQ GTFAKVYFGR SNHTNESVAI KMIDKDKVMR VGLSQQIKRE ISVMRIAKHP NVVELYEVMA TKSRIYFVIE YCKGGELFNK
101: VAKGKLKEDV AWKYFYQLIS AVDFCHSRGV YHRDIKPENL LLDDNDNLKV SDFGLSALAD CKRQDGLLHT TCGTPAYVAP EVINRKGYEG TKADIWSCGV
201: VLFVLLAGYL PFHDTNLMEM YRKIGKADFK CPSWFAPEVK RLLCKMLDPN HETRITIAKI KESSWFRKGL HLKQKKMEKM EKQQVREATN PMEAGGSGQN
301: ENGENHEPPR LATLNAFDII ALSTGFGLAG LFGDVYDKRE SRFASQKPAS EIISKLVEVA KCLKLKIRKQ GAGLFKLERV KEGKNGILTM DAEIFQVTPT
401: FHLVEVKKCN GDTMEYQKLV EEDLRPALAD IVWVWQGEKE KEEQLLQDEQ GEQEPS

Arabidopsis Description

CIPK2Non-specific serine/threonine protein kinase [Source:UniProtKB/TrEMBL;Acc:A0A178URC7]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.