Factsheet for Os03t0291500-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 2
plastid 4
mitochondrion 5

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS4A01G109900.1	Wheat	mitochondrion, plastid	84.27	86.13
TraesCS4D01G195100.1	Wheat	mitochondrion, plastid	83.77	85.76
TraesCS4B01G194400.2	Wheat	mitochondrion, plastid	83.77	85.76
Zm00001d028750_P002	Maize	extracellular	82.78	85.03
KXG39588	Sorghum	mitochondrion	82.62	84.58
HORVU4Hr1G056240.2	Barley	plastid	85.6	82.46
Zm00001d047736_P001	Maize	cytosol	75.0	79.61
Solyc06g007180.2.1	Tomato	cytosol, plastid	68.38	70.0
PGSC0003DMT400010685	Potato	cytosol	68.21	69.83
GSMUA_Achr9P04460_001	Banana	cytosol	65.56	69.6
KRH17053	Soybean	cytosol, endoplasmic reticulum, mitochondrion	66.39	69.02
KRH72702	Soybean	cytosol, endoplasmic reticulum	66.39	68.9
VIT_06s0004g06830.t01	Wine grape	cytosol	66.39	68.78
GSMUA_Achr5P28200_001	Banana	cytosol	67.55	68.57
KRH30252	Soybean	cytosol	65.56	68.39
KRH30266	Soybean	endoplasmic reticulum	65.4	68.22
KRG98262	Soybean	cytosol	65.4	68.22
AT3G47340.1	Thale cress	cytosol	65.4	67.64
Bra018160.1-P	Field mustard	cytosol	65.23	67.24
CDY71298	Canola	cytosol	61.76	67.21
Os06t0265000-01	Rice	cytosol	65.4	66.84
Bra019500.1-P	Field mustard	cytosol	65.4	66.72
CDY21785	Canola	cytosol	65.23	66.67
CDX99060	Canola	cytosol	64.57	66.67
CDX77846	Canola	cytosol	65.23	66.55
CDY51796	Canola	cytosol	23.01	61.78
Solyc04g055210.2.1	Tomato	plastid	18.38	55.78
VIT_00s0742g00040.t01	Wine grape	nucleus	3.64	45.83

Protein Annotations

KEGG:00250+6.3.5.4	Gene3D:3.40.50.620	Gene3D:3.60.20.10	MapMan:4.1.2.1.1	EntrezGene:4332506	ProteinID:ABF95401.1
EMBL:AC137634	EMBL:AK318591	InterPro:AsnB_N	InterPro:Asn_synth_AEB	InterPro:Asn_synthase	ProteinID:BAF11713.2
ProteinID:BAS83672.1	InterPro:GATase_2_dom	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004066
GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0006529	GO:GO:0006541	GO:GO:0008150
GO:GO:0008152	GO:GO:0008652	GO:GO:0009058	GO:GO:0009628	GO:GO:0009646	GO:GO:0009987
GO:GO:0016874	GO:GO:0042803	GO:GO:0070981	InterPro:IPR014729	InterPro:IPR017932	InterPro:IPR029055
InterPro:Ntn_hydrolases_N	EnsemblPlantsGene:Os03g0291500	EnsemblPlants:Os03t0291500-01	PFAM:PF00733	PFAM:PF13537	PIRSF:PIRSF001589
PFscan:PS51278	PANTHER:PTHR11772	PANTHER:PTHR11772:SF16	UniProt:Q10MX3	InterPro:Rossmann-like_a/b/a_fold	SUPFAM:SSF52402
SUPFAM:SSF56235	TIGRFAMs:TIGR01536	UniParc:UPI0000DB496A	RefSeq:XP_015632089.1	SEG:seg	:

Description

ASPARAGINE SYNTHETASE 1Asparagine synthetase, Biosynthesis of asparagine following the supply of ammonium (Os03t0291500-01)

Coordinates

chr3:-:10120289..10124384

Molecular Weight (calculated)

67325.9 Da

IEP (calculated)

6.511

GRAVY (calculated)

-0.298

Length

604 amino acids

Sequence
(BLAST)

001: MCGILAVLGA ADWSQAKRAH VLSCSRRLKH RGPDWSGLYQ CEGNFLAQQR LAIVSPLSGD QPLYNADRTI VVVANGEIYN HKKIRKQFAS KHTFSTGSDC
101: EVIIPLYEEY GEDFVDMLDG VFAFVLYDTR TKTYMAARDA IGVNPLYIGR GSDGAVWISS EMKALNEDCV EFEIFPPGHL YSSAAGGLRR WYKPQWFAEN
201: VPATPYQPLL LREAFEKAVI KRLMTDVPFG VLLSGGLDSS LVAAVTKRHL IKTEAAEKFG AELHSFVVGL EGSPDLIAAR EVADHLGTIH HEFHFTVQDG
301: IDAIEEVIYH DETYDVTTIR ASTPMFLMAR KIKALGVKMV LSGEGSDELL GGYLYFHFAP NKEEFHKETC RKVKALHQYD CLRANKATSA WGLEVRVPFL
401: DKEFINVAMS MDPEWKMYNA DLGRIEKWVM RKAFDDEEHP YLPKHILYRQ KEQFSDGVGY NWIDGLKAFT EQQVSDEMMK NAAKVYPHNT PVNKEAYYYR
501: MIFERLFPQE SARETVPWGP SIACSTPAAI EWVEQWKASH DPSGRLIASH NSASASANHT NHANANANGN SNGKANGNCA MAANGTNGVG LVVANGTANG
601: KMEA

Best Arabidopsis Sequence Match ( AT5G65010.1 )
(BLAST)

001: MCGILAVLGC IDNSQAKRSR IIELSRRLRH RGPDWSGLHC YEDCYLAHER LAIIDPTSGD QPLYNEDKTV AVTVNGEIYN HKILREKLKS HQFRTGSDCE
101: VIAHLYEEHG EEFIDMLDGM FAFVLLDTRD KSFIAARDAI GITPLYIGWG LDGSVWFASE MKALSDDCEQ FMSFPPGHIY SSKQGGLRRW YNPPWYNEQV
201: PSTPYDPLVL RNAFEKAVIK RLMTDVPFGV LLSGGLDSSL VAAVALRHLE KSEAARQWGS QLHTFCIGLQ GSPDLKAGRE VADYLGTRHH EFQFTVQDGI
301: DAIEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEILG GYLYFHKAPN KKEFHEETCR KIKALHQFDC LRANKSTSAW GVEARVPFLD
401: KEFLNVAMSI DPEWKLIKPD LGRIEKWVLR NAFDDEERPY LPKHILYRQK EQFSDGVGYS WIDGLKDHAN KHVSDTMLSN ASFVFPDNTP LTKEAYYYRT
501: IFEKFFPKSA ARATVPGGPS IACSTAKAVE WDATWSKNLD PSGRAALGVH VAAYEEDKAA AAAKAGSDLV DPLPKNGT

Arabidopsis Description

ASN2asparagine synthetase 2 [Source:TAIR;Acc:AT5G65010]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.