Factsheet for Os04t0653400-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 3
endoplasmic reticulum 6
vacuole 3
plasma membrane 3
golgi 3
plastid 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	plastid, secretory
BaCelLo:	secretory
EpiLoc:	plastid
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	endoplasmic reticulum
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	endoplasmic reticulum

plasma membrane: 18260611
plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES11568	Sorghum	plastid	89.06	89.19
Zm00001d026483_P001	Maize	plasma membrane	88.92	89.05
Zm00001d002024_P002	Maize	endoplasmic reticulum, peroxisome, plastid	89.64	88.62
TraesCS2B01G534200.1	Wheat	endoplasmic reticulum	87.63	88.01
HORVU2Hr1G074570.1	Barley	cytosol	6.04	87.5
TraesCS2D01G506600.1	Wheat	endoplasmic reticulum	86.91	87.28
HORVU2Hr1G074590.1	Barley	cytosol	5.61	86.67
HORVU2Hr1G040900.1	Barley	cytosol	7.91	85.94
TraesCS2A01G505800.2	Wheat	endoplasmic reticulum	86.33	85.71
Os08t0243500-01	Rice	cytosol	69.78	74.5
VIT_11s0052g00880.t01	Wine grape	cytosol, endoplasmic reticulum, peroxisome	72.37	71.35
Os09t0558900-01	Rice	golgi, peroxisome, plastid	72.66	70.73
KRH02867	Soybean	endoplasmic reticulum	71.08	69.87
KRH19026	Soybean	mitochondrion	70.65	69.45
CDY14183	Canola	cytosol, peroxisome, plastid	68.06	67.96
CDY28169	Canola	plastid	67.91	67.82
Bra010296.1-P	Field mustard	cytosol, endoplasmic reticulum, plastid	68.35	67.19
CDY51656	Canola	plastid	68.2	67.14
PGSC0003DMT400035801	Potato	plastid	69.06	67.13
Bra011174.1-P	Field mustard	cytosol, plastid	64.75	66.96
CDX72222	Canola	cytosol, endoplasmic reticulum, peroxisome, plastid	68.2	66.95
CDX68634	Canola	plastid	68.49	66.85
Solyc07g019460.2.1	Tomato	cytosol	68.63	66.71
CDY03543	Canola	plastid	67.91	66.48
Bra024095.1-P	Field mustard	endoplasmic reticulum, plastid	63.88	66.47
AT4G30210.3	Thale cress	plastid	68.35	61.37
CDY34997	Canola	mitochondrion	8.49	27.19
Os01t0733600-01	Rice	cytosol	24.46	27.16
Bra021223.1-P	Field mustard	mitochondrion	8.06	26.29

Protein Annotations

Gene3D:1.20.990.10	Gene3D:2.40.30.10	Gene3D:3.40.50.360	Gene3D:3.40.50.80	EntrezGene:4337244	MapMan:50.1.6
ProteinID:BAS91389.1	ProteinID:CAE01547.2	ProteinID:CAE03554.2	InterPro:FAD-binding_1	InterPro:Fd_Rdtase_FAD-bd	InterPro:Flavdoxin-like
InterPro:Flavodoxin/NO_synth	InterPro:Flavoprot_Pyr_Nucl_cyt_Rdtase	InterPro:Flavoprotein-like_sf	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0003958	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737
GO:GO:0005783	GO:GO:0005789	GO:GO:0008150	GO:GO:0008152	GO:GO:0010181	GO:GO:0016020
GO:GO:0016021	GO:GO:0016491	GO:GO:0050660	GO:GO:0050661	GO:GO:0055114	InterPro:IPR008254
InterPro:IPR017927	InterPro:IPR023173	InterPro:IPR029039	HAMAP:MF_03212	InterPro:NADPH_Cyt_P450_Rdtase_alpha	EnsemblPlantsGene:Os04g0653400
EnsemblPlants:Os04t0653400-01	InterPro:OxRdtase_FAD/NAD-bd	InterPro:P450R	PFAM:PF00175	PFAM:PF00258	PFAM:PF00667
PIRSF:PIRSF000208	PRINTS:PR00369	PRINTS:PR00371	PFscan:PS50902	PFscan:PS51384	PANTHER:PTHR19384
PANTHER:PTHR19384:SF107	UniProt:Q7X7K8	InterPro:Riboflavin_synthase-like_b-brl	SUPFAM:SSF52218	SUPFAM:SSF52343	SUPFAM:SSF63380
TMHMM:TMhelix	UniParc:UPI000021C63C	RefSeq:XP_015636692.1	SEG:seg	:	:

Description

cytochrome P450 reductase 1, NADPH-cytochrome P450 reductase 1Similar to NADPH-cytochrome P450 reductase (Fragment). (Os04t0653400-01)

Coordinates

chr4:+:33329793..33335864

Molecular Weight (calculated)

76176.0 Da

IEP (calculated)

4.899

GRAVY (calculated)

-0.244

Length

695 amino acids

Sequence
(BLAST)

001: MALALEAARS WAASVLPPEL AAAAGGDPLA ALAATAAALV AGVVILAVWF RSGGGAPPKA AAPPPRPPPV KIEADADADD GRKRVTVFFG TQTGTAEGFA
101: KAMAEEARAR YEKAVFKVVD LDDYAAEDEE YEEKLRKETI VLLFLATYGD GEPTDNAARF YKWFTEGKEK EVWLKDLKYA VFGLGNRQYE HFNKVAKVVD
201: ELLEEQGGKR LVPVGLGDDD QCIEDDFTAW KEQVWPELDQ LLRDEDDTTG ASTPYTAAIP EYRIVFIDKS DVSFQDKSWS LANGSGVIDI HHPVRSNVAV
301: RKELHKPASD RSCIHLEFDI SGTGLVYETG DHVGVYSENA IETVEQAEKL LDLSPDTFFS VHADAEDGSP RKGGGSLAPP FPSPCTLRTA LLRYADLLNS
401: PKKAALVALA AHASDLAEAE RLRFLASPAG KDEYSQWVVA SQRSLLEVMA AFPSAKPPLG VFFAAVAPRL QPRYYSISSS PKMAPSRIHV TCALVYGPTP
501: TGRIHQGVCS TWMKNAIPSE YSEECSWAPI YVRQSNFKLP ADPTTPIIMI GPGTGLAPFR GFLQERLALK QSGVELGNSV LFFGCRNRNM DYIYEDELQN
601: FIQEGALSEL IVAFSREGPA KEYVQHKMTE KATEIWNIVS QGGYIYVCGD AKGMARDVHR ALHTIVQEQG SLDSSKTESY VKSLQMDGRY LRDVW

Best Arabidopsis Sequence Match ( AT4G30210.3 )
(BLAST)

001: MKNMMNYKLK LCSVSKNSKG VSLSPTPHLT KPPTIHTERD LLLPSSSFFF LLLSSSSYNI YNAMSSSSSS STSMIDLMAA IIKGEPVIVS DPANASAYES
101: VAAELSSMLI ENRQFAMIVT TSIAVLIGCI VMLVWRRSGS GNSKRVEPLK PLVIKPREEE IDDGRKKVTI FFGTQTGTAE GFAKALGEEA KARYEKTRFK
201: IVDLDDYAAD DDEYEEKLKK EDVAFFFLAT YGDGEPTDNA ARFYKWFTEG NDRGEWLKNL KYGVFGLGNR QYEHFNKVAK VVDDILVEQG AQRLVQVGLG
301: DDDQCIEDDF TAWREALWPE LDTILREEGD TAVATPYTAA VLEYRVSIHD SEDAKFNDIN MANGNGYTVF DAQHPYKANV AVKRELHTPE SDRSCIHLEF
401: DIAGSGLTYE TGDHVGVLCD NLSETVDEAL RLLDMSPDTY FSLHAEKEDG TPISSSLPPP FPPCNLRTAL TRYACLLSSP KKSALVALAA HASDPTEAER
501: LKHLASPAGK DEYSKWVVES QRSLLEVMAE FPSAKPPLGV FFAGVAPRLQ PRFYSISSSP KIAETRIHVT CALVYEKMPT GRIHKGVCST WMKNAVPYEK
601: SENCSSAPIF VRQSNFKLPS DSKVPIIMIG PGTGLAPFRG FLQERLALVE SGVELGPSVL FFGCRNRRMD FIYEEELQRF VESGALAELS VAFSREGPTK
701: EYVQHKMMDK ASDIWNMISQ GAYLYVCGDA KGMARDVHRS LHTIAQEQGS MDSTKAEGFV KNLQTSGRYL RDVW

Arabidopsis Description

ATR2P450 reductase 2 [Source:TAIR;Acc:AT4G30210]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.