Factsheet for Os06t0473200-02

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Os02t0148100-01
Os06t0699400-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G40440.1	Os02t0148100-01	AT1G59580.1	19513235
AT5G40440.1	Os06t0699400-01	AT1G59580.1	19513235
AT5G40440.1	Os02t0148100-01	AT2G18170.1	19513235
AT5G40440.1	Os06t0699400-01	AT2G18170.1	19513235
AT5G40440.1	Os02t0148100-01	AT4G36450.1	19513235
AT5G40440.1	Os06t0699400-01	AT4G36450.1	19513235

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
HORVU5Hr1G125270.1	Barley	cytosol	89.01	84.49
GSMUA_Achr9P18710_001	Banana	cytosol	52.96	63.51
KXG24971	Sorghum	cytosol	90.42	61.38
TraesCS5D01G549600.1	Wheat	cytosol	88.73	60.23
HORVU5Hr1G125290.3	Barley	cytosol	88.73	60.23
TraesCS5B01G565100.3	Wheat	cytosol	82.54	55.81
Zm00001d013510_P002	Maize	cytosol	50.14	55.62
Zm00001d041092_P001	Maize	plasma membrane	90.99	54.47
VIT_14s0066g00670.t01	Wine grape	cytosol	72.68	49.81
Solyc03g019850.2.1	Tomato	cytosol	70.14	48.35
PGSC0003DMT400039329	Potato	cytosol	70.14	48.35
Bra025576.1-P	Field mustard	cytosol	69.58	47.68
CDY42585	Canola	cytosol	69.58	47.68
CDY50119	Canola	cytosol	69.58	47.68
KRH56582	Soybean	cytosol	69.58	47.68
AT5G40440.1	Thale cress	cytosol	69.01	47.12
KRG93261	Soybean	cytosol	69.58	46.96
Os01t0510100-01	Rice	cytosol, plasma membrane	36.9	36.9
Os11t0226100-00	Rice	cytosol	21.97	36.28
GSMUA_Achr9P18700_001	Banana	cytosol	21.41	33.19
Os06t0147800-01	Rice	cytosol	32.68	32.95
Os06t0191300-01	Rice	plasma membrane	30.14	31.29
Os03t0225100-02	Rice	mitochondrion	28.73	30.09
Os02t0787300-01	Rice	plasma membrane	31.27	30.08
Os06t0191500-01	Rice	cytosol, plastid	22.82	26.47
Os02t0694900-00	Rice	mitochondrion	25.35	22.22
Os02t0179000-00	Rice	cytosol	25.92	22.17
Os01t0284300-00	Rice	cytosol	21.69	20.98
Os03t0713500-00	Rice	cytosol, nucleus, plastid	25.35	19.44
Os02t0791700-00	Rice	plasma membrane	24.79	13.02
Os06t0486400-01	Rice	plasma membrane	25.35	12.99
Os07t0507300-01	Rice	cytosol	24.51	12.54
Os03t0114300-01	Rice	mitochondrion	24.79	11.88
Os10t0518800-01	Rice	plasma membrane	24.23	11.61
Os03t0755000-01	Rice	cytosol, mitochondrion, plastid	23.94	10.13
Os03t0754800-01	Rice	cytosol	0.0	0.0

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.1	Gene3D:3.30.200.20	UniProt:A0A0P0WWL4	ProteinID:BAS97756.1	GO:GO:0000165
GO:GO:0000166	GO:GO:0000187	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674
GO:GO:0004707	GO:GO:0004708	GO:GO:0004871	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005737	GO:GO:0006464	GO:GO:0006468
GO:GO:0006950	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150	GO:GO:0008152	GO:GO:0009605
GO:GO:0009607	GO:GO:0009719	GO:GO:0009737	GO:GO:0009738	GO:GO:0009814	GO:GO:0009864
GO:GO:0009866	GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0019538
InterPro:IPR000719	InterPro:Kinase-like_dom_sf	EnsemblPlantsGene:Os06g0473200	EnsemblPlants:Os06t0473200-02	PFAM:PF00069	PIRSF:PIRSF000654
ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24361	PANTHER:PTHR24361:SF487	InterPro:Prot_kinase_dom
InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00039393B4	:

Description

MITOGEN-ACTIVATED PROTEIN KINASE KINASE 3Similar to NPK2. (Os06t0473200-01);Similar to Mitogen-activated protein kinase kinase 3. (Os06t0473200-02)

Coordinates

chr6:-:15811011..15821769

Molecular Weight (calculated)

40132.3 Da

IEP (calculated)

6.371

GRAVY (calculated)

-0.167

Length

355 amino acids

Sequence
(BLAST)

001: SLGEYNINEH GFHKRSTGPE ESDSGEKAYR CASHDMHIFG PIGNGASSVV QRAVFIPVHR ILALKKINIF EKEKRQQILN EMRTLCEACC YIGLVEFQGA
101: FYMPDSGQIS IALEYMDGGS LANVIKIKKS IPEPVLAHML QKVLLGLRYL HEVRHLVHRD IKPANLLVNL KGEAKITDFG VSAGLDNTMA MCATFVGTVT
201: YMSPERIRNE NYSYAADIWS LGLAILECAT GKFPYNVNEG PANLMLQILD DPSPTPPKDS YSSEFCSFIN DCLQKDADAR PSCEQLLSHP FIKRYENTTV
301: DLVAYVKSIV DPTERLKQIA EMLAVHYYLL FNGTDGIWHY MKTFYMEEST FRYLW

Best Arabidopsis Sequence Match ( AT5G40440.4 )
(BLAST)

001: MAALEELKKK LSPLFDAEKG FSSSSSLDPN DSYLLSDGGT VNLLSRSYGV YNFNELGLQK CTSSHVDESE SSETTYQCAS HEMRVFGAIG SGASSVVQRA
101: IHIPNHRILA LKKINIFERE KRQQLLTEIR TLCEAPCHEG LVDFHGAFYS PDSGQISIAL EYMNGGSLAD ILKVTKKIPE PVLSSLFHKL LQGLSYLHGV
201: RHLVHRDIKP ANLLINLKGE PKITDFGISA GLENSMAMCA TFVGTVTYMS PERIRNDSYS YPADIWSLGL ALFECGTGEF PYIANEGPVN LMLQILDDPS
301: PTPPKQEFSP EFCSFIDACL QKDPDARPTA DQLLSHPFIT KHEKERVDLA TFVQSIFDPT QRLKDLADML TIHYYSLFDG FDDLWHHAKS LYTETSVFSF
401: SGKHNTGSTE IFSALSDIRN TLTGDLPSEK LVHVVEKLHC KPCGSGGVII RAVGSFIVGN QFLICGDGVQ AEGLPSFKDL GFDVASRRVG RFQEQFVVES
501: GDLIGKYFLA KQELYITNLD

Arabidopsis Description

MKK3Mitogen-activated protein kinase kinase 3 [Source:UniProtKB/Swiss-Prot;Acc:O80396]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.