Factsheet for PGSC0003DMT400039329

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 5
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	cytosol
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400009066
PGSC0003DMT400009491

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G40440.1	PGSC0003DMT400009491	AT1G59580.1	19513235
AT5G40440.1	PGSC0003DMT400009066	AT2G18170.1	19513235
AT5G40440.1	PGSC0003DMT400009066	AT4G36450.1	19513235

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc03g019850.2.1	Tomato	cytosol	99.22	99.22
KRH56582	Soybean	cytosol	82.52	82.05
VIT_14s0066g00670.t01	Wine grape	cytosol	81.94	81.47
KRG93261	Soybean	cytosol	82.72	80.99
GSMUA_Achr9P18710_001	Banana	cytosol	44.47	77.36
CDY50119	Canola	cytosol	75.92	75.48
CDY42585	Canola	cytosol	75.73	75.29
AT5G40440.1	Thale cress	cytosol	75.92	75.19
Bra025576.1-P	Field mustard	cytosol	75.53	75.1
Os06t0473200-02	Rice	cytosol	48.35	70.14
HORVU5Hr1G125270.1	Barley	cytosol	49.51	68.18
KXG24971	Sorghum	cytosol	68.74	67.69
Zm00001d013510_P002	Maize	cytosol	40.78	65.62
TraesCS5D01G549600.1	Wheat	cytosol	66.21	65.2
HORVU5Hr1G125290.3	Barley	cytosol	65.83	64.82
TraesCS5A01G122700.4	Wheat	cytosol	36.7	63.21
GSMUA_Achr9P18700_001	Banana	cytosol	27.57	62.01
Zm00001d041092_P001	Maize	plasma membrane	67.77	58.85
TraesCS5D01G130900.2	Wheat	cytosol	56.89	55.81
TraesCS5B01G122600.1	Wheat	cytosol	49.51	53.57
TraesCS5B01G565100.3	Wheat	cytosol	49.71	48.76
PGSC0003DMT400014637	Potato	cytosol	26.6	38.7
PGSC0003DMT400000744	Potato	cytosol	26.02	37.53
PGSC0003DMT400083995	Potato	cytosol	20.58	31.83
PGSC0003DMT400023739	Potato	plastid	21.94	30.54
PGSC0003DMT400074400	Potato	cytosol	16.12	22.25
Zm00001d004610_P001	Maize	cytosol	6.41	18.44
PGSC0003DMT400042095	Potato	cytosol	17.86	17.59
PGSC0003DMT400036874	Potato	cytosol	18.84	14.46
PGSC0003DMT400031593	Potato	nucleus	5.63	6.14

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:102603964	MapMan:18.4.2.1	Gene3D:3.10.450.50	Gene3D:3.30.200.20	GO:GO:0000165
GO:GO:0000166	GO:GO:0000187	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004707
GO:GO:0004708	GO:GO:0004871	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005634	GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0006950
GO:GO:0007154	GO:GO:0007165	GO:GO:0008150	GO:GO:0008152	GO:GO:0009605	GO:GO:0009607
GO:GO:0009719	GO:GO:0009737	GO:GO:0009738	GO:GO:0009814	GO:GO:0009864	GO:GO:0009866
GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:IPR018222
InterPro:Kinase-like_dom_sf	UniProt:M1B851	InterPro:NTF2-like_dom_sf	InterPro:Nuclear_transport_factor_2_euk	PFAM:PF00069	EnsemblPlantsGene:PGSC0003DMG402015209
PGSC:PGSC0003DMG402015209	EnsemblPlants:PGSC0003DMT400039329	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PFscan:PS50177
PANTHER:PTHR24361	PANTHER:PTHR24361:SF487	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF54427
SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0002966AF9	RefSeq:XP_006351529.1	:	:

Description

NPK2 [Source:PGSC_GENE;Acc:PGSC0003DMG402015209]

Coordinates

chr3:-:50244166..50250920

Molecular Weight (calculated)

57456.7 Da

IEP (calculated)

5.725

GRAVY (calculated)

-0.167

Length

515 amino acids

Sequence
(BLAST)

001: MAGLEELKKK LVPLFDADKG FSPTSTSDPF DSYSLSDAGT VNLLSQSYGV YNINELGLQK WPVDDTDHGE KTYRCASHEM RVFGAIGAGA SSVVQRAIHI
101: PTHRIIALKK INIFEKEKRQ QLLTEIRTLC EAPCYQGLVE FYGAFYTPDS GQISIALEYM DGGSLADIIK IRKSIPEPIL SSMVQKLLHG LSYLHGVRHL
201: VHRDIKPANL LVNLKGEPKI TDFGISAGLE SSIAMCATFV GTVTYMSPER IRNENYSYPA DIWSLGLALF ECGTGEFPYT ANEGPVNLML QILDDPSPSL
301: SRHDYSPEFC SFVDACLKKN PDDRPTADQL LSHPFIIKYS DSALDLGTFV RDIFDPTQRM KDLADMLTIH YYLLFDGSNE FWQHTKTLYN ECSTFSFGGK
401: ESIGPNNIFS TLSNIRNTLA GEWPPEKLVH VVEKLQCRAN GQDGVAIRVS GSFIVGNQFL ICGDGMQVEG LPNLKDLSID IPSKRMGTFH EQFIVEKANI
501: IGRYFITKHE LFITQ

Best Arabidopsis Sequence Match ( AT5G40440.4 )
(BLAST)

001: MAALEELKKK LSPLFDAEKG FSSSSSLDPN DSYLLSDGGT VNLLSRSYGV YNFNELGLQK CTSSHVDESE SSETTYQCAS HEMRVFGAIG SGASSVVQRA
101: IHIPNHRILA LKKINIFERE KRQQLLTEIR TLCEAPCHEG LVDFHGAFYS PDSGQISIAL EYMNGGSLAD ILKVTKKIPE PVLSSLFHKL LQGLSYLHGV
201: RHLVHRDIKP ANLLINLKGE PKITDFGISA GLENSMAMCA TFVGTVTYMS PERIRNDSYS YPADIWSLGL ALFECGTGEF PYIANEGPVN LMLQILDDPS
301: PTPPKQEFSP EFCSFIDACL QKDPDARPTA DQLLSHPFIT KHEKERVDLA TFVQSIFDPT QRLKDLADML TIHYYSLFDG FDDLWHHAKS LYTETSVFSF
401: SGKHNTGSTE IFSALSDIRN TLTGDLPSEK LVHVVEKLHC KPCGSGGVII RAVGSFIVGN QFLICGDGVQ AEGLPSFKDL GFDVASRRVG RFQEQFVVES
501: GDLIGKYFLA KQELYITNLD

Arabidopsis Description

MKK3Mitogen-activated protein kinase kinase 3 [Source:UniProtKB/Swiss-Prot;Acc:O80396]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.