Factsheet for Bra025576.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
cytosol 4
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
EpiLoc:	cytosol
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra035437.1-P
Bra037234.1-P
Bra039629.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G40440.1	Bra035437.1-P	AT1G59580.1	19513235
AT5G40440.1	Bra037234.1-P	AT2G18170.1	19513235
AT5G40440.1	Bra039629.1-P	AT2G18170.1	19513235

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY42585	Canola	cytosol	99.42	99.42
CDY50119	Canola	cytosol	98.65	98.65
AT5G40440.1	Thale cress	cytosol	92.86	92.5
KRH56582	Soybean	cytosol	79.15	79.15
VIT_14s0066g00670.t01	Wine grape	cytosol	78.38	78.38
KRG93261	Soybean	cytosol	79.15	77.95
Solyc03g019850.2.1	Tomato	cytosol	75.29	75.73
PGSC0003DMT400039329	Potato	cytosol	75.1	75.53
GSMUA_Achr9P18710_001	Banana	cytosol	43.05	75.34
Os06t0473200-02	Rice	cytosol	47.68	69.58
HORVU5Hr1G125270.1	Barley	cytosol	48.84	67.65
KXG24971	Sorghum	cytosol	66.22	65.58
TraesCS5A01G122700.4	Wheat	cytosol	37.45	64.88
GSMUA_Achr9P18700_001	Banana	cytosol	28.38	64.19
TraesCS5D01G549600.1	Wheat	cytosol	63.9	63.29
HORVU5Hr1G125290.3	Barley	cytosol	63.51	62.91
Zm00001d013510_P002	Maize	cytosol	38.42	62.19
Zm00001d041092_P001	Maize	plasma membrane	65.83	57.5
TraesCS5D01G130900.2	Wheat	cytosol	56.37	55.62
TraesCS5B01G122600.1	Wheat	cytosol	49.61	53.99
TraesCS5B01G565100.3	Wheat	cytosol	50.0	49.33
Bra002806.1-P	Field mustard	cytosol	26.83	39.04
Bra006866.1-P	Field mustard	cytosol	25.68	38.22
Bra011137.1-P	Field mustard	cytosol	26.83	38.19
Bra008099.1-P	Field mustard	mitochondrion	22.2	37.58
Bra003842.1-P	Field mustard	mitochondrion	21.81	36.69
Bra019122.1-P	Field mustard	cytosol	24.9	36.34
Bra001818.1-P	Field mustard	plastid	22.39	35.15
Bra020769.1-P	Field mustard	plastid	20.85	35.06
Bra023894.1-P	Field mustard	cytosol, plastid	22.01	34.44
Bra030430.1-P	Field mustard	cytosol, plastid	22.01	33.83
Bra014295.1-P	Field mustard	cytosol, plastid	23.17	33.8
Bra001817.1-P	Field mustard	cytosol	22.59	30.87
Bra023257.1-P	Field mustard	cytosol, mitochondrion, nucleus, plasma membrane, plastid	17.76	29.87
Bra006265.1-P	Field mustard	cytosol	9.85	26.56
Zm00001d004610_P001	Maize	cytosol	6.56	18.99
Bra039858.1-P	Field mustard	cytosol	16.99	18.22
Bra016220.1-P	Field mustard	cytosol	19.11	16.72
Bra007914.1-P	Field mustard	cytosol	19.11	16.23
Bra035282.1-P	Field mustard	cytosol	18.53	16.22
Bra035132.1-P	Field mustard	cytosol	18.53	16.16
Bra008742.1-P	Field mustard	cytosol	18.15	14.99
Bra023483.1-P	Field mustard	cytosol	18.15	14.92
Bra033169.1-P	Field mustard	cytosol	18.73	14.72
Bra027269.1-P	Field mustard	cytosol	18.92	14.43
Bra003600.1-P	Field mustard	cytosol	17.37	14.15
Bra013762.1-P	Field mustard	mitochondrion	18.53	14.08
Bra038106.1-P	Field mustard	plastid	19.5	12.14
Bra004366.1-P	Field mustard	cytosol	19.3	11.63

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.1	Gene3D:3.10.450.50	Gene3D:3.30.200.20	EnsemblPlantsGene:Bra025576	EnsemblPlants:Bra025576.1
EnsemblPlants:Bra025576.1-P	GO:GO:0000165	GO:GO:0000166	GO:GO:0000187	GO:GO:0003674	GO:GO:0003824
GO:GO:0004672	GO:GO:0004707	GO:GO:0004708	GO:GO:0004871	GO:GO:0005488	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005737	GO:GO:0006464
GO:GO:0006468	GO:GO:0006950	GO:GO:0007154	GO:GO:0007165	GO:GO:0008150	GO:GO:0008152
GO:GO:0009605	GO:GO:0009607	GO:GO:0009719	GO:GO:0009737	GO:GO:0009738	GO:GO:0009814
GO:GO:0009864	GO:GO:0009866	GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538
InterPro:IPR000719	InterPro:IPR018222	InterPro:Kinase-like_dom_sf	UniProt:M4E9X0	InterPro:NTF2-like_dom_sf	InterPro:Nuclear_transport_factor_2_euk
PFAM:PF00069	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PFscan:PS50177	PANTHER:PTHR24361
PANTHER:PTHR24361:SF487	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF54427	SUPFAM:SSF56112
InterPro:Ser/Thr_kinase_AS	UniParc:UPI0002544ECB	SEG:seg	:	:	:

Description

AT5G40440 (E=7e-276) ATMKK3, MKK3 | ATMKK3 (ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 3); MAP kinase kinase

Coordinates

chrA04:+:7742899..7745165

Molecular Weight (calculated)

57563.6 Da

IEP (calculated)

5.576

GRAVY (calculated)

-0.163

Length

518 amino acids

Sequence
(BLAST)

001: MAALEELKKK LSPLFDAEKG FSSSSSLDPN DSYLLSDGGT VNLLSRSYGV YNFNELGLQK CTTSHVDESE SSETTYQCAS YEMRVFGAIG SGASSVVQRA
101: IHIPKHRLLA LKRINIFERE KRQQLLTEIR TLCEAPCHEG LVDFHGAFYS PDSGQISIAL EYMDGGSLAD ILKVTKKIPE PVLSSMFHKL LQGLSYLHGV
201: RHLVHRDIKP ANLLVNLKGE PKITDFGISS GLENSMAMCA TFVGTVTYMS PERIRNDGYS YPADIWSLGL ALFECGTGEF PYIANEGPVN LMLQILDDPS
301: PTPSKQEFSP EFCSFIDACL QKDADARPTA DQLLSHPFIT KHEKESVDLA AFVRSIFDPT QRLKDIADML TIHYYSLFDG YDDLWHHIRS LYTETSVFSY
401: SGKQYTGSTE ILSQLSNIRN TLAGDLPSEK LVHVVEKLQC KPHGVGGFMI RAIGSFIVGN QFLICGDGVQ AEGLPSFKDL GFDLGTRRVG RFQEQFVVES
501: GDLIGRYFIA KQELYITN

Best Arabidopsis Sequence Match ( AT5G40440.4 )
(BLAST)

001: MAALEELKKK LSPLFDAEKG FSSSSSLDPN DSYLLSDGGT VNLLSRSYGV YNFNELGLQK CTSSHVDESE SSETTYQCAS HEMRVFGAIG SGASSVVQRA
101: IHIPNHRILA LKKINIFERE KRQQLLTEIR TLCEAPCHEG LVDFHGAFYS PDSGQISIAL EYMNGGSLAD ILKVTKKIPE PVLSSLFHKL LQGLSYLHGV
201: RHLVHRDIKP ANLLINLKGE PKITDFGISA GLENSMAMCA TFVGTVTYMS PERIRNDSYS YPADIWSLGL ALFECGTGEF PYIANEGPVN LMLQILDDPS
301: PTPPKQEFSP EFCSFIDACL QKDPDARPTA DQLLSHPFIT KHEKERVDLA TFVQSIFDPT QRLKDLADML TIHYYSLFDG FDDLWHHAKS LYTETSVFSF
401: SGKHNTGSTE IFSALSDIRN TLTGDLPSEK LVHVVEKLHC KPCGSGGVII RAVGSFIVGN QFLICGDGVQ AEGLPSFKDL GFDVASRRVG RFQEQFVVES
501: GDLIGKYFLA KQELYITNLD

Arabidopsis Description

MKK3Mitogen-activated protein kinase kinase 3 [Source:UniProtKB/Swiss-Prot;Acc:O80396]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.