Factsheet for Bra023894.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid, cytosol

Predictor Summary:

nucleus 2
plastid 4
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra000326.1-P
Bra004784.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G21220.1	Bra000326.1-P	AT2G43790.1	19513235
AT3G21220.1	Bra004784.1-P	AT2G43790.1	19513235

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY37306	Canola	cytosol, plastid	99.4	99.4
CDY27532	Canola	cytosol, plastid	97.28	98.47
AT3G21220.1	Thale cress	cytosol, plastid	89.73	85.34
Bra001818.1-P	Field mustard	plastid	84.29	84.55
Bra030430.1-P	Field mustard	cytosol, plastid	72.81	71.51
Bra014295.1-P	Field mustard	cytosol, plastid	75.53	70.42
Bra001817.1-P	Field mustard	cytosol	76.44	66.75
Solyc03g123800.1.1	Tomato	nucleus	71.9	66.3
HORVU7Hr1G031720.2	Barley	cytosol	53.47	66.29
GSMUA_Achr9P13590_001	Banana	mitochondrion	46.83	65.96
PGSC0003DMT400023739	Potato	plastid	73.11	65.41
KRH47006	Soybean	cytosol, plastid	67.07	63.25
KRH44646	Soybean	cytosol, plastid	68.28	62.09
GSMUA_Achr4P05870_001	Banana	cytosol, mitochondrion, plasma membrane, plastid	61.63	61.45
GSMUA_Achr9P22810_001	Banana	cytosol	23.57	60.94
OQU83236	Sorghum	cytosol	23.26	60.63
Os06t0191300-01	Rice	plasma membrane	62.54	60.53
KXG19514	Sorghum	plastid	63.44	60.52
TraesCS7B01G059000.1	Wheat	plastid	62.24	59.71
Zm00001d045064_P001	Maize	extracellular, plasma membrane	62.54	59.65
TraesCS7A01G154900.1	Wheat	plastid	62.24	59.03
TraesCS7D01G155100.1	Wheat	plastid	62.24	59.03
HORVU5Hr1G067100.2	Barley	cytosol	40.79	58.19
Zm00001d018326_P001	Maize	plastid	62.54	58.15
TraesCS6B01G379500.1	Wheat	cytosol, plastid	65.26	58.06
Os02t0787300-01	Rice	plasma membrane	64.65	57.99
TraesCS6D01G328800.1	Wheat	cytosol, plastid	64.35	57.88
Zm00001d052066_P001	Maize	plastid	63.14	57.42
OQU85840	Sorghum	plastid	64.35	57.26
VIT_09s0018g01820.t01	Wine grape	plastid	72.51	57.14
HORVU0Hr1G038850.2	Barley	cytosol	47.13	52.88
Bra008099.1-P	Field mustard	mitochondrion	45.92	49.67
Bra003842.1-P	Field mustard	mitochondrion	45.02	48.38
GSMUA_Achr3P05000_001	Banana	plastid	64.65	47.14
Bra020769.1-P	Field mustard	plastid	42.9	46.1
Os06t0191500-01	Rice	cytosol, plastid	41.39	44.77
Bra023257.1-P	Field mustard	cytosol, mitochondrion, nucleus, plasma membrane, plastid	35.35	37.99
Bra002806.1-P	Field mustard	cytosol	37.16	34.55
Bra006866.1-P	Field mustard	cytosol	35.95	34.2
Bra019122.1-P	Field mustard	cytosol	35.65	33.24
Bra011137.1-P	Field mustard	cytosol	35.35	32.14
Bra006265.1-P	Field mustard	cytosol	15.11	26.04
Bra025576.1-P	Field mustard	cytosol	34.44	22.01
Bra039858.1-P	Field mustard	cytosol	24.77	16.98
Bra035132.1-P	Field mustard	cytosol	24.17	13.47
Bra008742.1-P	Field mustard	cytosol	25.38	13.4
Bra023483.1-P	Field mustard	cytosol	24.77	13.02
Bra035282.1-P	Field mustard	cytosol	23.26	13.01
Bra016220.1-P	Field mustard	cytosol	23.26	13.01
Bra007914.1-P	Field mustard	cytosol	22.96	12.46
Bra033169.1-P	Field mustard	cytosol	24.47	12.29
Bra027269.1-P	Field mustard	cytosol	24.17	11.78
Bra013762.1-P	Field mustard	mitochondrion	23.87	11.58
Bra004366.1-P	Field mustard	cytosol	29.91	11.51
Bra003600.1-P	Field mustard	cytosol	21.75	11.32
Bra038106.1-P	Field mustard	plastid	25.08	9.98

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.1	Gene3D:3.30.200.20	EnsemblPlantsGene:Bra023894	EnsemblPlants:Bra023894.1	EnsemblPlants:Bra023894.1-P
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488
GO:GO:0005524	GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987
GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:Kinase-like_dom_sf	UniProt:M4E540
PFAM:PF00069	PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24361
PANTHER:PTHR24361:SF319	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS
UniParc:UPI000254470A	SEG:seg	:	:	:	:

Description

AT3G21220 (E=3e-160) ATMKK5, ATMAP2K_ALPHA, MAP2K_A, MKK5, ATMEK5, MEK5 | ATMKK5 (ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5); MAP kinase kinase/ kinase

Coordinates

chrA01:-:20887823..20888818

Molecular Weight (calculated)

36658.0 Da

IEP (calculated)

8.048

GRAVY (calculated)

-0.297

Length

331 amino acids

Sequence
(BLAST)

001: MNPIQPPPSP MKNRFRKRPD LSLPLPHRDV ALAVPLPLPP PPSTTSAPTS GSAISPNVSA AKSLSELERV NRIGSGAGGT VYKVIHRPTS KPFALKVIYG
101: NHEDNVRRQI CREIEILRSV DHANVVKCHD MFDHNGEIQV LLEFMDGGCL EGVHVSREEE LSDMSRQILS GLAYLHRHHI VHRDIKPSNL LIDSEKNVKI
201: ADFGVSRILA QTMDPCNSSV GTIAYMSPER INTDLNHGRY NGYAGDVWSL GVSILEFYLG RFPFAVSRQG DWASLMCAIC MSQPPEAPAT ASEEFRHFVS
301: CCLQSDPPKR WSAQQLLQHP FILKSTRAPG S

Best Arabidopsis Sequence Match ( AT3G21220.2 )
(BLAST)

001: MKPIQSPSGV ASPMKNRLRK RPDLSLPLPH RDVALAVPLP LPPPSSSSSA PASSSAISTN ISAAKSLSEL ERVNRIGSGA GGTVYKVIHT PTSRPFALKV
101: IYGNHEDTVR RQICREIEIL RSVDHPNVVK CHDMFDHNGE IQVLLEFMDQ GSLEGAHIWQ EQELADLSRQ ILSGLAYLHR RHIVHRDIKP SNLLINSAKN
201: VKIADFGVSR ILAQTMDPCN SSVGTIAYMS PERINTDLNH GRYDGYAGDV WSLGVSILEF YLGRFPFAVS RQGDWASLMC AICMSQPPEA PATASQEFRH
301: FVSCCLQSDP PKRWSAQQLL QHPFILKATG GPNLRQMLPP PRPLPSAS

Arabidopsis Description

MKK5MKK5 [Source:UniProtKB/TrEMBL;Acc:A0A178VIK5]
SUBAcon: [plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.