Factsheet for Os02t0787300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
cytosol 4
mitochondrion 2
plastid 2

Predictors

GFP

MS/MS

Papers

cytosol: 24320692
nucleus: 24320692

plasma membrane: 18260611
plastid: 21433289
plasma membrane: 27800704
plasma membrane: 28056797

gfp PMID: 24320692 doi

P Duan, Y Rao, D Zeng, Y Yang, R Xu, B Zhang, G Dong, Q Qian, Y Li

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China; University of Chinese Academy of Sciences, Beijing, 100039, China.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

msms PMID: 21433289 doi

S Reiland, J Grossmann, K Baerenfaller, P Gehrig, A Nunes-Nesi, AR Fernie, W Gruissem, S Baginsky

Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0621300-01
Os02t0127700-02
Os02t0258300-01
Os03t0285800-01
Os04t0654700-02
Os05t0586400-01
Os06t0154500-01
Os06t0524300-01
Os11t0104866-00
Os11t0104900-00
Os12t0104766-00
Os12t0104800-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G51660.1	Os02t0127700-02	AT1G32380.1	21798944
AT1G51660.1	Os04t0654700-02	AT1G71230.1	21798944
AT1G51660.1	Os06t0154500-01	AT2G43790.1	19513235
AT1G51660.1	Os06t0154500-01	AT2G43790.1	21798944
AT1G51660.1	Os11t0104866-00	AT3G08530.1	21798944
AT1G51660.1	Os11t0104900-00	AT3G08530.1	21798944
AT1G51660.1	Os12t0104766-00	AT3G08530.1	21798944
AT1G51660.1	Os12t0104800-01	AT3G08530.1	21798944
AT1G51660.1	Os03t0285800-01	AT3G45640.1	19513235
AT1G51660.1	Os03t0285800-01	AT3G45640.1	21798944
AT1G51660.1	Os01t0621300-01	AT4G01090.1	21798944
AT1G51660.1	Os02t0258300-01	AT4G01090.1	21798944
AT1G51660.1	Os05t0586400-01	AT4G01090.1	21798944
AT1G51660.1	Os06t0524300-01	AT4G01090.1	21798944
AT3G21220.1	Os06t0154500-01	AT2G43790.1	19513235

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d018326_P001	Maize	plastid	85.09	88.2
OQU85840	Sorghum	plastid	88.62	87.9
TraesCS6B01G379500.1	Wheat	cytosol, plastid	88.62	87.9
TraesCS6D01G328800.1	Wheat	cytosol, plastid	86.72	86.96
Zm00001d052066_P001	Maize	plastid	84.01	85.16
Os06t0191300-01	Rice	plasma membrane	77.24	83.33
HORVU5Hr1G067100.2	Barley	cytosol	47.7	75.86
GSMUA_Achr9P13590_001	Banana	mitochondrion	47.15	74.04
HORVU0Hr1G038850.2	Barley	cytosol	57.72	72.2
CDY32415	Canola	cytosol, plastid	45.8	70.71
Bra001818.1-P	Field mustard	plastid	58.27	65.15
CDY27532	Canola	cytosol, plastid	57.72	65.14
Bra023894.1-P	Field mustard	cytosol, plastid	57.99	64.65
CDY37306	Canola	cytosol, plastid	57.99	64.65
CDX82209	Canola	plastid	57.45	64.24
CDX87830	Canola	cytosol	37.4	64.19
AT3G21220.1	Thale cress	cytosol, plastid	59.35	62.93
CDY43920	Canola	cytosol, plastid	57.18	62.61
KRH47006	Soybean	cytosol, plastid	59.35	62.39
Bra030430.1-P	Field mustard	cytosol, plastid	56.91	62.31
CDY46547	Canola	cytosol, plastid	59.89	62.25
Solyc03g123800.1.1	Tomato	nucleus	60.43	62.12
CDY52951	Canola	cytosol	59.89	62.08
Bra014295.1-P	Field mustard	cytosol, plastid	59.62	61.97
CDX82210	Canola	plastid	51.22	61.76
PGSC0003DMT400023739	Potato	plastid	61.25	61.08
AT1G51660.1	Thale cress	plastid	60.16	60.66
KRH44646	Soybean	cytosol, plastid	59.35	60.16
CDY32414	Canola	extracellular, plasma membrane	54.74	55.8
GSMUA_Achr3P05000_001	Banana	plastid	68.02	55.29
VIT_09s0018g01820.t01	Wine grape	plastid	61.25	53.81
Bra001817.1-P	Field mustard	cytosol	53.39	51.98
Os06t0191500-01	Rice	cytosol, plastid	42.55	51.31
Os03t0225100-02	Rice	mitochondrion	37.94	41.3
Os01t0510100-01	Rice	cytosol, plasma membrane	33.33	34.65
Os06t0147800-01	Rice	cytosol	30.89	32.39
Os06t0473200-02	Rice	cytosol	30.08	31.27
Os11t0226100-00	Rice	cytosol	18.16	31.16
Os02t0694900-00	Rice	mitochondrion	33.06	30.12
Os03t0713500-00	Rice	cytosol, nucleus, plastid	35.77	28.51
Os01t0284300-00	Rice	cytosol	25.47	25.61
Os02t0179000-00	Rice	cytosol	23.31	20.72
Os02t0791700-00	Rice	plasma membrane	23.31	12.72
Os07t0507300-01	Rice	cytosol	23.58	12.54
Os06t0486400-01	Rice	plasma membrane	23.31	12.41
Os03t0755000-01	Rice	cytosol, mitochondrion, plastid	27.64	12.16
Os03t0114300-01	Rice	mitochondrion	21.95	10.93
Os10t0518800-01	Rice	plasma membrane	21.14	10.53
Os03t0754800-01	Rice	cytosol	0.54	0.62

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.1	Gene3D:3.30.200.20	EntrezGene:4330957	EMBL:AK120525	ProteinID:BAD19216.1
ProteinID:BAD19853.1	ProteinID:BAS81283.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672
GO:GO:0004674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006464	GO:GO:0006468	GO:GO:0006950	GO:GO:0007049	GO:GO:0007154
GO:GO:0007165	GO:GO:0007346	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016310	GO:GO:0016740	GO:GO:0019538	GO:GO:0023014	GO:GO:0031098	GO:GO:0032147
InterPro:IPR000719	InterPro:Kinase-like_dom_sf	EnsemblPlantsGene:Os02g0787300	EnsemblPlants:Os02t0787300-01	PFAM:PF00069	PIRSF:PIRSF000654
ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24361	PANTHER:PTHR24361:SF469	InterPro:Prot_kinase_dom
InterPro:Protein_kinase_ATP_BS	UniProt:Q6K4Q0	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00003757D0
RefSeq:XP_015627603.1	SEG:seg	:	:	:	:

Description

SMALL GRAIN 1Mitogen-activated protein kinase kinase 4, Defense response, Cell proliferation, Grain growth (Os02t0787300-01)

Coordinates

chr2:-:33442070..33443948

Molecular Weight (calculated)

39945.8 Da

IEP (calculated)

9.557

GRAVY (calculated)

-0.405

Length

369 amino acids

Sequence
(BLAST)

001: MRPGGPPSLR AGLQQQQQQQ PGTPGRSRRR PDLTLPLPQR DLTSLAVPLP LPLPPSSAPS STSSSGSSSL GGVPTPPNSV GSAPPAPPPL SELERVRRIG
101: SGAGGTVWMV RHRPTGRPYA LKVLYGNHDD AVRRQITREI AILRTAEHPA VVRCHGMYEQ AGELQILLEY MDGGSLEGRR IASEAFLADV ARQVLSGIAY
201: LHRRHIVHRD IKPSNLLIDS GRRVKIADFG VGRILNQTMD PCNSSVGTIA YMSPERINTD LNDGAYDGYA GDIWSFGLSI LEFYMGRFPL GENLGKQGDW
301: AALMCAICYS DSPAPPPNAS PEFKSFISCC LQKNPARRPS AAQLLQHRFV AGPQQQQQPQ PQPLAPPPS

Best Arabidopsis Sequence Match ( AT3G21220.2 )
(BLAST)

001: MKPIQSPSGV ASPMKNRLRK RPDLSLPLPH RDVALAVPLP LPPPSSSSSA PASSSAISTN ISAAKSLSEL ERVNRIGSGA GGTVYKVIHT PTSRPFALKV
101: IYGNHEDTVR RQICREIEIL RSVDHPNVVK CHDMFDHNGE IQVLLEFMDQ GSLEGAHIWQ EQELADLSRQ ILSGLAYLHR RHIVHRDIKP SNLLINSAKN
201: VKIADFGVSR ILAQTMDPCN SSVGTIAYMS PERINTDLNH GRYDGYAGDV WSLGVSILEF YLGRFPFAVS RQGDWASLMC AICMSQPPEA PATASQEFRH
301: FVSCCLQSDP PKRWSAQQLL QHPFILKATG GPNLRQMLPP PRPLPSAS

Arabidopsis Description

MKK5MKK5 [Source:UniProtKB/TrEMBL;Acc:A0A178VIK5]
SUBAcon: [plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.