Factsheet for Os06t0191300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
plasma membrane 2
mitochondrion 3
extracellular 1
endoplasmic reticulum 1
vacuole 1
golgi 1
cytosol 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, nucleus, secretory
BaCelLo:	nucleus
MultiLoc:	plasma membrane
Plant-mPloc:	nucleus
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	mitochondrion
WoLF PSORT:	mitochondrion
YLoc:	cytosol

plasma membrane: 28056797

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0621300-01
Os02t0127700-02
Os02t0258300-01
Os03t0285800-01
Os04t0654700-02
Os05t0586400-01
Os06t0154500-01
Os06t0524300-01
Os11t0104866-00
Os11t0104900-00
Os12t0104766-00
Os12t0104800-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G51660.1	Os02t0127700-02	AT1G32380.1	21798944
AT1G51660.1	Os04t0654700-02	AT1G71230.1	21798944
AT1G51660.1	Os06t0154500-01	AT2G43790.1	19513235
AT1G51660.1	Os06t0154500-01	AT2G43790.1	21798944
AT1G51660.1	Os11t0104866-00	AT3G08530.1	21798944
AT1G51660.1	Os11t0104900-00	AT3G08530.1	21798944
AT1G51660.1	Os12t0104766-00	AT3G08530.1	21798944
AT1G51660.1	Os12t0104800-01	AT3G08530.1	21798944
AT1G51660.1	Os03t0285800-01	AT3G45640.1	19513235
AT1G51660.1	Os03t0285800-01	AT3G45640.1	21798944
AT1G51660.1	Os01t0621300-01	AT4G01090.1	21798944
AT1G51660.1	Os02t0258300-01	AT4G01090.1	21798944
AT1G51660.1	Os05t0586400-01	AT4G01090.1	21798944
AT1G51660.1	Os06t0524300-01	AT4G01090.1	21798944
AT3G21220.1	Os06t0154500-01	AT2G43790.1	19513235

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os02t0787300-01	Rice	plasma membrane	83.33	77.24
GSMUA_Achr9P13590_001	Banana	mitochondrion	47.95	69.79
CDY32415	Canola	cytosol, plastid	46.49	66.53
CDX87830	Canola	cytosol	39.77	63.26
CDY27532	Canola	cytosol, plastid	60.23	63.0
Bra001818.1-P	Field mustard	plastid	60.53	62.73
Bra023894.1-P	Field mustard	cytosol, plastid	60.53	62.54
CDY37306	Canola	cytosol, plastid	60.53	62.54
CDX82209	Canola	plastid	59.65	61.82
AT3G21220.1	Thale cress	cytosol, plastid	62.57	61.49
CDX82210	Canola	plastid	54.09	60.46
KRH47006	Soybean	cytosol, plastid	61.99	60.4
CDY46547	Canola	cytosol, plastid	61.7	59.44
CDY43920	Canola	cytosol, plastid	58.48	59.35
Bra014295.1-P	Field mustard	cytosol, plastid	61.4	59.15
Solyc03g123800.1.1	Tomato	nucleus	61.99	59.05
Bra030430.1-P	Field mustard	cytosol, plastid	58.19	59.05
CDY52951	Canola	cytosol	61.11	58.71
KRH44646	Soybean	cytosol, plastid	62.28	58.52
AT1G51660.1	Thale cress	plastid	62.57	58.47
PGSC0003DMT400023739	Potato	plastid	62.57	57.84
CDY32414	Canola	extracellular, plasma membrane	57.31	54.14
GSMUA_Achr3P05000_001	Banana	plastid	71.34	53.74
Os06t0191500-01	Rice	cytosol, plastid	47.95	53.59
VIT_09s0018g01820.t01	Wine grape	plastid	63.45	51.67
Bra001817.1-P	Field mustard	cytosol	54.68	49.34
Os03t0225100-02	Rice	mitochondrion	41.23	41.59
Os01t0510100-01	Rice	cytosol, plasma membrane	35.09	33.8
Os06t0147800-01	Rice	cytosol	33.63	32.67
Os11t0226100-00	Rice	cytosol	19.59	31.16
Os06t0473200-02	Rice	cytosol	31.29	30.14
Os02t0694900-00	Rice	mitochondrion	35.09	29.63
Os03t0713500-00	Rice	cytosol, nucleus, plastid	38.01	28.08
Os01t0284300-00	Rice	cytosol	25.73	23.98
Os02t0179000-00	Rice	cytosol	25.73	21.2
Os02t0791700-00	Rice	plasma membrane	25.44	12.87
Os06t0486400-01	Rice	plasma membrane	25.73	12.7
Os07t0507300-01	Rice	cytosol	24.27	11.96
Os03t0755000-01	Rice	cytosol, mitochondrion, plastid	27.49	11.2
Os10t0518800-01	Rice	plasma membrane	23.98	11.07
Os03t0114300-01	Rice	mitochondrion	23.98	11.07
Os03t0754800-01	Rice	cytosol	0.29	0.31

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.1	Gene3D:3.30.200.20	EntrezGene:4340367	ProteinID:BAD35809.1	ProteinID:BAS96569.1
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0006464
GO:GO:0006468	GO:GO:0006950	GO:GO:0007049	GO:GO:0007154	GO:GO:0007165	GO:GO:0007346
GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740
GO:GO:0019538	GO:GO:0023014	GO:GO:0031098	GO:GO:0032147	InterPro:IPR000719	InterPro:Kinase-like_dom_sf
EnsemblPlantsGene:Os06g0191300	EnsemblPlants:Os06t0191300-01	PFAM:PF00069	PIRSF:PIRSF000654	ScanProsite:PS00107	ScanProsite:PS00108
PFscan:PS50011	PANTHER:PTHR24361	PANTHER:PTHR24361:SF469	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	UniProt:Q69TJ8
SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI00004038D8	RefSeq:XP_015643444.1	SEG:seg

Description

MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5Similar to Mitogen-activated protein kinase kinase 4 (Fragment). (Os06t0191300-01);Similar to Mitogen-activated protein kinase kinase 4 (Fragment). (Os06t0191300-02)

Coordinates

chr6:+:4616608..4618145

Molecular Weight (calculated)

37106.6 Da

IEP (calculated)

8.462

GRAVY (calculated)

-0.301

Length

342 amino acids

Sequence
(BLAST)

001: MRAGDMPGRG ARRRPDLTLP MPQRDAPTSL AVPLPLPPAA TTTTSAPPAG GAMHPLASAG AAPPPPLEEL ERVRRVGSGA GGTVWMVRHR GTGKEYALKV
101: LYGNHDDAVR RQIAREIAIL RTAEHPAVVR CHDMYERGGE LQILLEYMDG GSLDGRRIAD ERFLADVARQ VLSGIAYLHR RHIVHRDIKP SNLLIDSARR
201: VKIADFGVGR ILNQTMDPCN SSVGTIAYMS PERINTDLND GAYDGYAGDI WSFGLSILEF YMGKFPFGEN LGKQGDWAAL MCAICYSDPP EPPAAVSPEF
301: RSFVGYCLQK NPAKRPSAAQ LMQHPFVAGP QPQPLAAPPP SS

Best Arabidopsis Sequence Match ( AT1G51660.1 )
(BLAST)

001: MRPIQSPPGV SVPVKSRPRR RPDLTLPLPQ RDVSLAVPLP LPPTSGGSGG SSGSAPSSGG SASSTNTNSS IEAKNYSDLV RGNRIGSGAG GTVYKVIHRP
101: SSRLYALKVI YGNHEETVRR QICREIEILR DVNHPNVVKC HEMFDQNGEI QVLLEFMDKG SLEGAHVWKE QQLADLSRQI LSGLAYLHSR HIVHRDIKPS
201: NLLINSAKNV KIADFGVSRI LAQTMDPCNS SVGTIAYMSP ERINTDLNQG KYDGYAGDIW SLGVSILEFY LGRFPFPVSR QGDWASLMCA ICMSQPPEAP
301: ATASPEFRHF ISCCLQREPG KRRSAMQLLQ HPFILRASPS QNRSPQNLHQ LLPPPRPLSS SSSPTT

Arabidopsis Description

MKK4MKK4 [Source:UniProtKB/TrEMBL;Acc:A0A178WCC0]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.