Factsheet for PGSC0003DMT400069148

Subcellular Localization

min:

: max

Winner_takes_all: golgi, endoplasmic reticulum

Predictor Summary:

cytosol 1
extracellular 4
golgi 5
plasma membrane 4
endoplasmic reticulum 6
vacuole 4
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum, golgi
Any Predictor:	cytosol, plastid, secretory
BaCelLo:	cytosol
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	endoplasmic reticulum
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	golgi

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc06g073570.2.1	Tomato	extracellular, golgi	95.6	95.95
VIT_00s0189g00110.t01	Wine grape	cytosol	20.0	59.24
PGSC0003DMT400082322	Potato	cytosol	11.74	58.72
VIT_12s0035g00570.t01	Wine grape	cytosol	24.04	56.96
PGSC0003DMT400011829	Potato	endoplasmic reticulum, extracellular	49.17	50.57
PGSC0003DMT400072524	Potato	endoplasmic reticulum, extracellular	47.52	49.43
PGSC0003DMT400051969	Potato	endoplasmic reticulum, extracellular	45.69	47.88
PGSC0003DMT400004789	Potato	cytosol	18.17	47.83
VIT_09s0002g09270.t01	Wine grape	endoplasmic reticulum	41.28	47.47
VIT_09s0002g09250.t01	Wine grape	endoplasmic reticulum, golgi	44.77	47.1
VIT_09s0002g09200.t01	Wine grape	extracellular, golgi	44.4	46.72
VIT_12s0035g00610.t01	Wine grape	extracellular, golgi	44.22	46.44
PGSC0003DMT400075815	Potato	endoplasmic reticulum, extracellular	44.59	46.11
PGSC0003DMT400039871	Potato	cytosol	39.08	45.71
VIT_00s1216g00020.t01	Wine grape	endoplasmic reticulum, extracellular	43.12	45.37
VIT_12s0035g00560.t01	Wine grape	endoplasmic reticulum, extracellular	37.43	42.68
KRH07450	Soybean	endoplasmic reticulum, extracellular	40.73	42.61
PGSC0003DMT400008625	Potato	endoplasmic reticulum, golgi	39.63	41.78
PGSC0003DMT400038700	Potato	mitochondrion	38.9	41.49
PGSC0003DMT400085819	Potato	cytosol	11.38	41.33
PGSC0003DMT400004790	Potato	endoplasmic reticulum, golgi	39.27	41.31
PGSC0003DMT400008624	Potato	extracellular, golgi	38.35	40.9
PGSC0003DMT400008622	Potato	endoplasmic reticulum, golgi	37.06	40.89
PGSC0003DMT400008634	Potato	endoplasmic reticulum, extracellular	38.9	40.69
PGSC0003DMT400008635	Potato	endoplasmic reticulum, extracellular	37.8	40.31
PGSC0003DMT400056767	Potato	endoplasmic reticulum	38.17	39.92
VIT_00s1216g00010.t01	Wine grape	mitochondrion	7.71	28.38
VIT_04s0044g01610.t01	Wine grape	endoplasmic reticulum, extracellular	42.2	22.35

Protein Annotations

Gene3D:1.10.630.10	EntrezGene:102589007	MapMan:50.1.13	ncoils:Coil	InterPro:Cyt_P450	InterPro:Cyt_P450_CS
InterPro:Cyt_P450_E_grp-I	InterPro:Cyt_P450_sf	GO:GO:0003674	GO:GO:0003824	GO:GO:0004497	GO:GO:0005488
GO:GO:0005506	GO:GO:0005575	GO:GO:0008150	GO:GO:0008152	GO:GO:0016020	GO:GO:0016021
GO:GO:0016491	GO:GO:0016705	GO:GO:0020037	GO:GO:0046872	GO:GO:0055114	InterPro:IPR036396
UniProt:M1CK00	PFAM:PF00067	EnsemblPlantsGene:PGSC0003DMG400026900	PGSC:PGSC0003DMG400026900	EnsemblPlants:PGSC0003DMT400069148	PRINTS:PR00385
PRINTS:PR00463	ScanProsite:PS00086	PANTHER:PTHR24298	PANTHER:PTHR24298:SF223	SUPFAM:SSF48264	TMHMM:TMhelix
UniParc:UPI000296DAD8	RefSeq:XP_006347265.2	SEG:seg	:	:	:

Description

Cytochrome P450 CYP82E4v2 nicotine demethylase [Source:PGSC_GENE;Acc:PGSC0003DMG400026900]

Coordinates

chr6:-:54071342..54074897

Molecular Weight (calculated)

62593.1 Da

IEP (calculated)

7.408

GRAVY (calculated)

-0.204

Length

545 amino acids

Sequence
(BLAST)

001: MEIHILSHVE AIIGILVFVF LFFLIRKAKT WKNSKKFGPP PPQLPGAWPI IGHLPQILSD TLDGRNRSNT DGEIPLAHTL AALADKYGPV FTIRQGMFPI
101: AVVSSYEAIK ECFTTQDKNL ANRVATCSCK YLGYDHANLT FANYGPYWRM VRKLVVNNLL SSKSLERLKH VRISEVESSI KELYTLFVAN EAKNVLTKVD
201: IGHWFDDMML NITVKMIGGK RYSQVNNKEE EKEEAERFRK AFNETMYYIA MVGIEDAFPI PLLQWLDLQG NIKVMKRIAD EMDVILQRWL DDHTNKRKNN
301: DSNDDDDQDL IDIMLTELDK DDFQYGYSRE TIIKATMLTI VSDATHTTAV HLIWIIACLL NNKHVLKKVH EEIDTKVGKN RWVEDLDIKN LTYFQATIKE
401: VLRLYPPSPM LVHEALADCQ VLGYHISKGT RLFVNVWKLQ KDSKFWPEPE KFLPERFLTT KAKVDVYGKD LEFIPFGSGR RSCPGITMAM QVTYLSIARL
501: LQAFDFETPN NEPVDMTEGP GFTAVKKIPL EVVVKPRMLP MYYGV

Best Arabidopsis Sequence Match ( AT2G25160.1 )
(BLAST)

001: MDLIMLFLLS ALFIFPVLIL IKSRLRPKNK KSTAPMVPGA WPLLGHLHLF DTVNPTHVTF GAMADVYGPV FMAKLGSIKV MIINSKEVAK EIYTVHDKLL
101: ERPELTASKL LGYNDSFLTF SPYGLYWREI RKIAVSELFS TSGVDMHMVS RAREADLAFR ALYVRWEKRG KPKEGVLVDM KQEFIDLTAN ISLMMVSGKR
201: YFGENPNCEV KEARRCGKLI REFLDYFALF LLSDVAPVLG FLDWKTKRGM KRTAKGLDKV AEGWIEEHKN KRSDHGRSEN DYLDILIKIL GQDKIPGLSD
301: THTKIKALCL NLVLAGSETA IVVLVWAVSL LLNNPHVLRK AQEELDSKIG KERVVEELDI KDLVYLQAIV KETFRLYPPV PLVAYRAVVE DFDIAFCKCH
401: VPAGTQLMVS AWKIHRDPNV WSNPEQFEPE RFLTSNRELD VGGQSYKFFP FGLGRRSCPA IPLGMRMVHY LLVRFLHSFD LARPSSQDVD MTESNGLVNH
501: KATPLEVNII PRLHKSLYEV DHIGTDN

Arabidopsis Description

CYP82F1At2g25160 [Source:UniProtKB/TrEMBL;Acc:Q7Y222]
SUBAcon: [golgi,plasma membrane,endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.