Factsheet for Bra014201.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plastid, cytosol

Predictor Summary:

nucleus 3
plastid 2
cytosol 2
mitochondrion 1

Predictors

GFP

MS/MS

Papers

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra012987.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G49580.1	Bra012987.1-P	AT5G61010.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY16510	Canola	cytosol, plastid	95.76	98.49
CDX77233	Canola	cytosol, plastid	94.62	98.14
AT1G49580.1	Thale cress	cytosol	87.77	88.78
Bra037539.1-P	Field mustard	cytosol, plastid	77.81	80.3
Bra022388.1-P	Field mustard	cytosol	77.81	80.03
Os07t0619800-02	Rice	cytosol, plasma membrane	56.12	75.94
TraesCS2D01G173100.1	Wheat	cytosol	57.75	74.37
VIT_03s0063g00940.t01	Wine grape	cytosol	67.05	70.38
KRH50248	Soybean	cytosol	67.86	69.57
Bra001734.1-P	Field mustard	cytosol, plastid	74.06	69.53
KRH71170	Soybean	cytosol	67.7	69.4
Solyc01g108400.2.1	Tomato	cytosol	66.07	68.88
GSMUA_Achr1P21380_001	Banana	cytosol	67.86	68.53
PGSC0003DMT400066365	Potato	cytosol	65.58	68.37
Bra036089.1-P	Field mustard	cytosol	65.09	67.17
GSMUA_Achr2P07660_001	Banana	cytosol	65.25	65.9
EER99712	Sorghum	cytosol	64.44	65.72
TraesCS2A01G165800.1	Wheat	cytosol, plastid	64.27	65.34
Zm00001d006885_P002	Maize	cytosol	61.17	65.33
TraesCS2B01G191800.1	Wheat	cytosol	64.11	65.07
Bra000215.1-P	Field mustard	cytosol	57.59	62.26
Bra004600.1-P	Field mustard	cytosol	58.56	62.22
Zm00001d022166_P001	Maize	cytosol	56.44	60.38
Bra014673.1-P	Field mustard	cytosol, nucleus, plasma membrane	56.44	60.17
Zm00001d000221_P001	Maize	cytosol	11.09	59.65
Bra016950.1-P	Field mustard	cytosol	45.68	56.91
Bra039263.1-P	Field mustard	cytosol	55.14	56.71
Bra017989.1-P	Field mustard	cytosol	52.2	54.15
Bra036512.1-P	Field mustard	cytosol	50.9	53.79
Bra009765.1-P	Field mustard	cytosol	48.29	52.02
HORVU2Hr1G030520.1	Barley	cytosol	40.13	49.7
Bra019593.1-P	Field mustard	cytosol	51.55	48.54
Bra012058.1-P	Field mustard	plastid	39.8	45.95
Bra037181.1-P	Field mustard	plastid	39.15	44.61
Bra017746.1-P	Field mustard	cytosol	38.01	43.07
Bra024487.1-P	Field mustard	plastid	40.46	42.69
Bra002237.1-P	Field mustard	cytosol	29.36	37.66
Bra026489.1-P	Field mustard	cytosol	28.71	36.21
Bra020040.1-P	Field mustard	cytosol	31.48	36.14
Bra008879.1-P	Field mustard	cytosol	30.67	35.34
Bra004656.1-P	Field mustard	cytosol	30.67	35.34
Bra016909.1-P	Field mustard	cytosol	30.51	35.22
Bra002324.1-P	Field mustard	cytosol	28.22	35.02
Bra012835.1-P	Field mustard	cytosol	30.02	34.85
Bra006881.1-P	Field mustard	cytosol	30.02	34.85
Bra033476.1-P	Field mustard	cytosol	29.85	34.66
Bra031055.1-P	Field mustard	cytosol, plastid	30.02	34.65
Bra003287.1-P	Field mustard	cytosol	30.34	34.57
Bra015896.1-P	Field mustard	cytosol	30.51	34.25
Bra007334.1-P	Field mustard	cytosol	30.18	34.2
Bra011605.1-P	Field mustard	cytosol	29.85	33.58
Bra025696.1-P	Field mustard	cytosol, plastid	28.71	33.33
Bra022844.1-P	Field mustard	cytosol, plastid	29.53	32.91
Bra021727.1-P	Field mustard	plasma membrane	30.51	31.75
Bra018236.1-P	Field mustard	plastid	30.02	31.51
Bra018243.1-P	Field mustard	cytosol	20.39	29.14
Bra002099.1-P	Field mustard	plastid	40.29	27.63

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	MapMan:18.4.5.5	Gene3D:3.30.200.20	EnsemblPlantsGene:Bra014201	EnsemblPlants:Bra014201.1
EnsemblPlants:Bra014201.1-P	InterPro:EF-hand-dom_pair	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005623	GO:GO:0005886	GO:GO:0006464
GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016020	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:Kinase-like_dom_sf	UniProt:M4DCI7	PFAM:PF00069
ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24349	PANTHER:PTHR24349:SF115	InterPro:Prot_kinase_dom
InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF47473	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0002546361
SEG:seg	:	:	:	:	:

Description

AT1G49580 (E=3e-304) | calcium-dependent protein kinase, putative / CDPK, putative

Coordinates

chrA08:-:2563736..2566497

Molecular Weight (calculated)

68482.6 Da

IEP (calculated)

8.281

GRAVY (calculated)

-0.369

Length

613 amino acids

Sequence
(BLAST)

001: MGGCTSKPSP SGRPDNPFFP GNDYPLIDNS TPTNPTPSAA KASPFFPFYT PSPARHRRNK SRDVTGGESK SVTSTPLRQL RRAFNPPSPA KHIRAALRRR
101: KGKKEAALSA EATEAPPPEA GEEGLSAEAT EAPPQEEGEE VGLDKRFGFA RDLHSRVELG DEIGRGHFGY TCSAKFRKGE LKGQVVAVKI IPKSKMTSAI
201: AIEDVRREVK ILQALSGHKN LVQFYDAFED NANVYIAMEL CEGGELLDRI LARGGKYSED DAKPVIIQIL NVVAFCHLHG VVHRDLKPEN FLYTSKEENS
301: QLKAIDFGLS DFVKPDERLN DIVGSAYYVA PEVLHRSYTT EADVWSIGVI AYILLCGSRP FWARTESGIF RAVLKADPSF DEAPWPFLSS EAKDFVKRLL
401: FKDPRRRMSA SQALMHPWIR GYDRDMNVPF DILIFRQMKA YLRSSSLRKA ALRALSKTLI TDEIIYLKTQ FSFLSPNKDG FITLGSIRSA LSSNATDEMK
501: ETRIPEFLAL LNGLQHRGMD FEEFCAAAIN VHQHESLDCW EESIRHAYEL FERNGNRAIV IEELASELGV GPAITVHSVL HDWIRHTDGK LSFFGFAKLL
601: HGVSVRPSVK TTR

Best Arabidopsis Sequence Match ( AT1G49580.1 )
(BLAST)

001: MGGCTSKPST SSGRPNPFAP GNDYPQIDDF APDHPGKSPI PTPSAAKASP FFPFYTPSPA RHRRNKSRDV GGGGESKSLT STPLRQLRRA FHPPSPAKHI
101: RAALRRRKGK KEAALSGVTQ LTTEVPQREE EEEVGLDKRF GFSKEFHSRV ELGEEIGRGH FGYTCSAKFK KGELKGQVVA VKIIPKSKMT TAIAIEDVRR
201: EVKILQALSG HKNLVQFYDA FEDNANVYIA MELCEGGELL DRILARGGKY SENDAKPVII QILNVVAFCH FQGVVHRDLK PENFLYTSKE ENSQLKAIDF
301: GLSDFVRPDE RLNDIVGSAY YVAPEVLHRS YTTEADVWSI GVIAYILLCG SRPFWARTES GIFRAVLKAD PSFDEPPWPF LSSDAKDFVK RLLFKDPRRR
401: MSASQALMHP WIRAYNTDMN IPFDILIFRQ MKAYLRSSSL RKAALRALSK TLIKDEILYL KTQFSLLAPN KDGLITMDTI RMALASNATE AMKESRIPEF
501: LALLNGLQYR GMDFEEFCAA AINVHQHESL DCWEQSIRHA YELFDKNGNR AIVIEELASE LGVGPSIPVH SVLHDWIRHT DGKLSFFGFV KLLHGVSVRA
601: SGKTTR

Arabidopsis Description

CRK8CDPK-related kinase 8 [Source:UniProtKB/Swiss-Prot;Acc:Q9FX86]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.