Factsheet for Bra004600.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
plastid 1
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
ChloroP:	plastid
EpiLoc:	nucleus
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra040179.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G41140.1	Bra040179.1-P	AT4G17750.1	18466301

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX79956	Canola	cytosol	99.48	99.48
CDY23873	Canola	cytosol	98.61	98.44
Bra000215.1-P	Field mustard	cytosol	93.24	94.89
AT2G41140.1	Thale cress	cytosol	93.24	93.4
Bra014673.1-P	Field mustard	cytosol, nucleus, plasma membrane	86.66	86.96
KRG95088	Soybean	cytosol	61.01	84.62
KRH33063	Soybean	endoplasmic reticulum, vacuole	48.01	81.23
GSMUA_Achr6P16020_001	Banana	cytosol	59.97	80.65
Bra016950.1-P	Field mustard	cytosol	68.63	80.49
Os07t0641200-01	Rice	plasma membrane	36.22	79.77
KRH71707	Soybean	cytosol	80.59	79.76
Solyc10g078390.1.1	Tomato	cytosol	79.03	79.44
PGSC0003DMT400021598	Potato	mitochondrion	78.51	78.92
HORVU7Hr1G066710.3	Barley	cytosol	23.22	78.36
HORVU6Hr1G016380.1	Barley	cytosol	22.88	77.19
HORVU3Hr1G026700.1	Barley	plasma membrane	18.54	76.43
VIT_08s0040g01250.t01	Wine grape	cytosol, plastid, vacuole	78.34	76.22
Os03t0366200-01	Rice	cytosol, plasma membrane	38.13	76.12
GSMUA_Achr10P...	Banana	cytosol	75.74	75.87
EER99788	Sorghum	cytosol	76.78	74.7
TraesCS2A01G146200.2	Wheat	cytosol	76.43	74.62
TraesCS2D01G150800.1	Wheat	cytosol	76.43	74.37
TraesCS2B01G171600.1	Wheat	cytosol	76.43	74.37
GSMUA_Achr6P23680_001	Banana	cytosol	73.83	74.35
GSMUA_Achr3P18430_001	Banana	cytosol	68.8	73.65
HORVU5Hr1G097280.1	Barley	extracellular, plasma membrane	11.96	73.4
EER94810	Sorghum	cytosol	72.27	70.44
HORVU1Hr1G005350.3	Barley	endoplasmic reticulum, plasma membrane	23.4	69.95
Bra036089.1-P	Field mustard	cytosol	71.92	69.87
Zm00001d047428_P001	Maize	cytosol	71.4	68.9
Bra037539.1-P	Field mustard	cytosol, plastid	64.99	63.13
Zm00001d022305_P001	Maize	cytosol	64.99	62.92
Bra022388.1-P	Field mustard	cytosol	64.47	62.42
Zm00001d007029_P001	Maize	cytosol	64.47	62.21
Zm00001d029264_P004	Maize	cytosol	71.75	61.79
Bra014201.1-P	Field mustard	cytosol, plastid	62.22	58.56
Bra039263.1-P	Field mustard	cytosol	59.27	57.38
Bra036512.1-P	Field mustard	cytosol	57.02	56.72
Bra017989.1-P	Field mustard	cytosol	57.02	55.67
Bra001734.1-P	Field mustard	cytosol, plastid	62.39	55.13
Bra009765.1-P	Field mustard	cytosol	53.55	54.31
HORVU2Hr1G025890.1	Barley	cytosol	71.4	51.5
Bra019593.1-P	Field mustard	cytosol	56.67	50.23
Bra012058.1-P	Field mustard	plastid	41.25	44.82
Bra037181.1-P	Field mustard	plastid	40.55	43.49
Bra024487.1-P	Field mustard	plastid	43.67	43.37
Bra017746.1-P	Field mustard	cytosol	40.21	42.88
Bra002237.1-P	Field mustard	cytosol	32.06	38.7
Bra020040.1-P	Field mustard	cytosol	34.32	37.08
Bra008879.1-P	Field mustard	cytosol	33.8	36.65
Bra026489.1-P	Field mustard	cytosol	30.68	36.42
Bra002324.1-P	Field mustard	cytosol	30.85	36.03
Bra003287.1-P	Field mustard	cytosol	33.28	35.69
Bra007334.1-P	Field mustard	cytosol	33.1	35.3
Bra031055.1-P	Field mustard	cytosol, plastid	32.41	35.22
Bra006881.1-P	Field mustard	cytosol	31.89	34.85
Bra033476.1-P	Field mustard	cytosol	31.72	34.66
Bra015896.1-P	Field mustard	cytosol	32.58	34.43
Bra012835.1-P	Field mustard	cytosol	31.37	34.28
Bra016909.1-P	Field mustard	cytosol	31.37	34.09
Bra004656.1-P	Field mustard	cytosol	31.37	34.02
Bra011605.1-P	Field mustard	cytosol	32.06	33.95
Bra025696.1-P	Field mustard	cytosol, plastid	31.02	33.9
Bra022844.1-P	Field mustard	cytosol, plastid	31.89	33.45
Bra018236.1-P	Field mustard	plastid	32.06	31.68
Bra021727.1-P	Field mustard	plasma membrane	32.24	31.58
Bra018243.1-P	Field mustard	cytosol	20.8	27.97
Bra002099.1-P	Field mustard	plastid	43.33	27.96

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	MapMan:18.4.5.5	Gene3D:3.30.200.20	EnsemblPlantsGene:Bra004600	EnsemblPlants:Bra004600.1
EnsemblPlants:Bra004600.1-P	InterPro:EF-hand-dom_pair	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672
GO:GO:0005488	GO:GO:0005524	GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016301	GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:Kinase-like_dom_sf
UniProt:M4CK66	PFAM:PF00069	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24349
PANTHER:PTHR24349:SF92	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF47473	SUPFAM:SSF56112
InterPro:Ser/Thr_kinase_AS	UniParc:UPI000254759D	SEG:seg	:	:	:

Description

AT2G41140 (E=3e-292) CRK1, ATCRK1, ATCBK3 | CRK1 (CDPK-RELATED KINASE 1); calcium ion binding / calcium-dependent protein serine/threonine phosphatase/ calmodulin-dependent protein kinase/ kinase

Coordinates

chrA05:+:985597..988672

Molecular Weight (calculated)

64418.6 Da

IEP (calculated)

8.502

GRAVY (calculated)

-0.255

Length

577 amino acids

Sequence
(BLAST)

001: MGMCHGKPIE HQSKQSLPAP DEPQPPPPPP PAKSSGFPFY SPSPLPSLFK SSPAVSSSAS STPLRIFKRP FPPPSPAKHI RAFLARRHGS SAKPPSEVSI
101: PEGEEFEIGL DKSFGFSKHF ASHYEIDGEV GRGHFGYTCS AKGKKGSLKG QEVAVKVIPK SKMTTAIAIE DVSREVKILR ALTGHKNLVQ FYDAFEDEEN
201: VYIVMELCKG GELLDKILQR GGKYSEDDAK QVMVQILSVV AYCHLQGVVH RDLKPENFLF STKDETSPLK AIDFGLSDYV KPDERLNDIV GSAYYVAPEV
301: LHRTYGTEAD MWSIGVIAYI LLCGSRPFWA RTESGIFRAV LKAEPNFEEA PWPSLSPDAV DFVKRLLNKD YRKRLTAAQA LCHPWLVGSH ELKIPSDMII
401: YKLVKVYIMS TSLRKSALQA LAKTLTVPQL AYLREQFTLL GPSKNGYISM QNYKTAIVKT ATDATKDSRV LDFVHMISCL QYRKLDFEEF CASALSVYQL
501: EAMETWEQHA RRAYELFEKD GNRPIMIEEL ASELGLGPSV PVHVVLQDWI RHSDGKLSFL GFVRLLHGVS SRTLHKA

Best Arabidopsis Sequence Match ( AT2G41140.1 )
(BLAST)

001: MGICHGKPVE QQSKSLPVSG ETNEAPTNSQ PPAKSSGFPF YSPSPVPSLF KSSPSVSSSV SSTPLRIFKR PFPPPSPAKH IRAFLARRYG SVKPNEVSIP
101: EGKECEIGLD KSFGFSKQFA SHYEIDGEVG RGHFGYTCSA KGKKGSLKGQ EVAVKVIPKS KMTTAIAIED VSREVKMLRA LTGHKNLVQF YDAFEDDENV
201: YIVMELCKGG ELLDKILQRG GKYSEDDAKK VMVQILSVVA YCHLQGVVHR DLKPENFLFS TKDETSPLKA IDFGLSDYVK PDERLNDIVG SAYYVAPEVL
301: HRTYGTEADM WSIGVIAYIL LCGSRPFWAR TESGIFRAVL KAEPNFEEAP WPSLSPEAVD FVKRLLNKDY RKRLTAAQAL CHPWLVGSHE LKIPSDMIIY
401: KLVKVYIMST SLRKSALAAL AKTLTVPQLA YLREQFTLLG PSKNGYISMQ NYKTAILKSS TDAMKDSRVF DFVHMISCLQ YKKLDFEEFC ASALSVYQLE
501: AMETWEQHAR RAYELFEKDG NRPIMIEELA SELGLGPSVP VHVVLQDWIR HSDGKLSFLG FVRLLHGVSS RTLQKA

Arabidopsis Description

CRK1CDPK-related kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:O80673]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.