Factsheet for PGSC0003DMT400021598

Subcellular Localization

min:

: max

Winner_takes_all: mitochondrion

Predictor Summary:

plastid 1
nucleus 2
plasma membrane 1
mitochondrion 1
cytosol 2

Predictors

GFP

MS/MS

Papers

mitochondrion: 24351685

msms PMID: 24351685 doi

F Salvato, JF Havelund, M Chen, RS Rao, A Rogowska-Wrzesinska, ON Jensen, DR Gang, JJ Thelen, IM Møller

Department of Biochemistry and Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400038381
PGSC0003DMT400046000

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G41140.1	PGSC0003DMT400038381	AT4G17750.1	18466301
AT2G41140.1	PGSC0003DMT400046000	AT4G17750.1	18466301

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc10g078390.1.1	Tomato	cytosol	99.13	99.13
KRG95088	Soybean	cytosol	61.5	84.86
KRH33063	Soybean	endoplasmic reticulum, vacuole	49.13	82.7
GSMUA_Achr6P16020_001	Banana	cytosol	60.8	81.35
VIT_08s0040g01250.t01	Wine grape	cytosol, plastid, vacuole	83.97	81.28
KRH71707	Soybean	cytosol	80.84	79.59
Bra000215.1-P	Field mustard	cytosol	78.05	79.01
CDY35427	Canola	cytosol	78.05	79.01
HORVU7Hr1G066710.3	Barley	cytosol	23.52	78.95
HORVU6Hr1G016380.1	Barley	cytosol	23.52	78.95
CDY22505	Canola	cytosol	78.05	78.87
AT2G41140.1	Thale cress	cytosol	79.09	78.82
Bra004600.1-P	Field mustard	cytosol	78.92	78.51
CDX79956	Canola	cytosol	78.92	78.51
AT3G56760.1	Thale cress	cytosol	78.75	78.34
GSMUA_Achr10P...	Banana	cytosol	78.57	78.3
CDY23873	Canola	cytosol	78.22	77.68
Os03t0366200-01	Rice	cytosol, plasma membrane	38.68	76.82
GSMUA_Achr6P23680_001	Banana	cytosol	76.66	76.79
CDY47375	Canola	cytosol, nucleus, plasma membrane	76.48	76.48
Os07t0641200-01	Rice	plasma membrane	34.67	75.95
CDX76010	Canola	cytosol	75.09	75.61
Bra014673.1-P	Field mustard	cytosol, nucleus, plasma membrane	75.61	75.48
HORVU3Hr1G026700.1	Barley	plasma membrane	18.29	75.0
TraesCS2A01G146200.2	Wheat	cytosol	77.18	74.96
EER99788	Sorghum	cytosol	77.18	74.7
TraesCS2D01G150800.1	Wheat	cytosol	77.0	74.54
TraesCS2B01G171600.1	Wheat	cytosol	77.0	74.54
GSMUA_Achr3P18430_001	Banana	cytosol	69.69	74.21
HORVU5Hr1G097280.1	Barley	extracellular, plasma membrane	11.85	72.34
EER94810	Sorghum	cytosol	73.87	71.62
Zm00001d047428_P001	Maize	cytosol	73.52	70.57
HORVU1Hr1G005350.3	Barley	endoplasmic reticulum, plasma membrane	23.69	70.47
Bra016950.1-P	Field mustard	cytosol	59.93	69.92
CDY57585	Canola	cytosol, mitochondrion	50.0	69.32
PGSC0003DMT400001990	Potato	cytosol	70.38	68.47
PGSC0003DMT400066365	Potato	cytosol	69.16	67.52
CDY06943	Canola	cytosol	54.18	66.74
Zm00001d022305_P001	Maize	cytosol	66.03	63.59
PGSC0003DMT400026076	Potato	cytosol	70.73	63.34
Zm00001d007029_P001	Maize	cytosol	65.51	62.88
Zm00001d029264_P004	Maize	cytosol	72.65	62.24
PGSC0003DMT400008340	Potato	cytosol	58.71	56.35
HORVU2Hr1G025890.1	Barley	cytosol	70.73	50.75
PGSC0003DMT400009177	Potato	plastid	43.03	43.79
PGSC0003DMT400058126	Potato	plastid	42.51	42.96
PGSC0003DMT400021055	Potato	cytosol	35.19	38.55
PGSC0003DMT400027809	Potato	cytosol	34.67	37.34
PGSC0003DMT400034291	Potato	cytosol	34.84	37.17
PGSC0003DMT400083474	Potato	cytosol	33.97	36.65
PGSC0003DMT400069175	Potato	plastid	32.75	35.07

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	EntrezGene:102591187	MapMan:18.4.5.5	Gene3D:3.30.200.20	InterPro:EF-hand-dom_pair
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0005488	GO:GO:0005524
GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:Kinase-like_dom_sf	UniProt:M1AFE1	PFAM:PF00069
EnsemblPlantsGene:PGSC0003DMG400008377	PGSC:PGSC0003DMG400008377	EnsemblPlants:PGSC0003DMT400021598	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PANTHER:PTHR24349	PANTHER:PTHR24349:SF92	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF47473
SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI000295545B	RefSeq:XP_006362272.1	SEG:seg	:

Description

Protein kinase; Calcium-binding EF-hand [Source:PGSC_GENE;Acc:PGSC0003DMG400008377]

Coordinates

chr10:-:59258871..59264872

Molecular Weight (calculated)

64281.1 Da

IEP (calculated)

8.812

GRAVY (calculated)

-0.287

Length

574 amino acids

Sequence
(BLAST)

001: MGLCHGKPIQ SPQNLSENPT IPIDNEQELN SHTGKTPKFP FYSPSPLPSA FKNSPANSSV TSTPLRFLKR VPPSPAKHIR ALLARRHGSI KPNEATIPEG
101: SECDIGLDKI FGYSKNFDSH YDLGEEVGRG HFGYTSAAKG KRGSLKGHDV AVKVIPKSKM TTAIAIEDVR REVKILRALT GHRNLVQFYD AYEDEENVYI
201: VMELCKGGEL LDRILARGGK YSEDDAKAVM VQLLSVVSYC HLQGVVHRDL KPENFLFVSK DENSPLKAID FGLSDYVKPD ERLNDIVGSA YYVAPEVLHR
301: SYGTEADMWS IGVIAYILLC GSRPFWARTE SGIFRAILKT DPNFDESPWP SLSSDAVDFV KRLLNKDYRK RLTAAQALSH PWLAGHHDVK IPLDMIVYKL
401: VKAYVYSSSL RKTALRALAK TLAIPQLAYL RDQFTLLGPS KSGLVALPNF KMAVMKNSTD AMKDSRVFDF VNTVSSLQYR KLDFEEFCAA SISVHQLEGM
501: ESWEQHARRG YEFFEKEGNR PIMIEELASE LGLSPSVPVH VVLQDWIRHS DGKLSFLGFV RLLHGVSSRT FQKA

Best Arabidopsis Sequence Match ( AT2G41140.1 )
(BLAST)

001: MGICHGKPVE QQSKSLPVSG ETNEAPTNSQ PPAKSSGFPF YSPSPVPSLF KSSPSVSSSV SSTPLRIFKR PFPPPSPAKH IRAFLARRYG SVKPNEVSIP
101: EGKECEIGLD KSFGFSKQFA SHYEIDGEVG RGHFGYTCSA KGKKGSLKGQ EVAVKVIPKS KMTTAIAIED VSREVKMLRA LTGHKNLVQF YDAFEDDENV
201: YIVMELCKGG ELLDKILQRG GKYSEDDAKK VMVQILSVVA YCHLQGVVHR DLKPENFLFS TKDETSPLKA IDFGLSDYVK PDERLNDIVG SAYYVAPEVL
301: HRTYGTEADM WSIGVIAYIL LCGSRPFWAR TESGIFRAVL KAEPNFEEAP WPSLSPEAVD FVKRLLNKDY RKRLTAAQAL CHPWLVGSHE LKIPSDMIIY
401: KLVKVYIMST SLRKSALAAL AKTLTVPQLA YLREQFTLLG PSKNGYISMQ NYKTAILKSS TDAMKDSRVF DFVHMISCLQ YKKLDFEEFC ASALSVYQLE
501: AMETWEQHAR RAYELFEKDG NRPIMIEELA SELGLGPSVP VHVVLQDWIR HSDGKLSFLG FVRLLHGVSS RTLQKA

Arabidopsis Description

CRK1CDPK-related kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:O80673]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.