Factsheet for PGSC0003DMT400072411

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

cytosol 3
plastid 4
mitochondrion 2

Predictors

GFP

MS/MS

Papers

cytosol: 22313117

msms PMID: 22313117 doi

S Lim, K Chisholm, RH Coffin, RD Peters, KI Al-Mughrabi, G Wang-Pruski, DM Pinto

Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Truro, Nova Scotia, Canada.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400031768

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G36580.1	PGSC0003DMT400031768	AT1G63970.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc10g083720.1.1	Tomato	plastid	99.2	93.93
PGSC0003DMT400006945	Potato	mitochondrion	88.38	88.38
VIT_08s0007g04170.t01	Wine grape	plastid	89.98	87.35
KRH67815	Soybean	cytosol, endoplasmic reticulum	89.98	85.2
TraesCS5A01G131100.1	Wheat	golgi	89.58	84.82
TraesCS5B01G131000.1	Wheat	golgi, unclear	89.38	84.63
HORVU5Hr1G041120.7	Barley	cytosol	89.38	84.31
Os12t0145700-01	Rice	plasma membrane	88.78	84.06
CDX73585	Canola	cytosol, plastid	88.78	84.06
AT3G52990.1	Thale cress	cytosol, plastid	88.58	83.87
Os11t0148500-02	Rice	plasma membrane	88.58	83.87
CDX78139	Canola	cytosol, plastid	88.58	83.87
Bra006965.1-P	Field mustard	cytosol, plastid	88.58	83.87
EES09257	Sorghum	cytosol, peroxisome, plastid	88.38	83.68
CDY58286	Canola	cytosol, plastid	88.38	83.68
CDX76243	Canola	cytosol, plastid	88.38	83.68
KRH20011	Soybean	nucleus	88.38	83.68
KRH32634	Soybean	endoplasmic reticulum	88.38	83.68
Bra019634.1-P	Field mustard	cytosol, plastid	88.38	83.68
GSMUA_Achr11P...	Banana	plastid	86.17	83.66
Bra028545.1-P	Field mustard	cytosol, plastid	88.18	83.49
CDY13321	Canola	cytosol, plastid	87.98	83.3
CDX67554	Canola	cytosol, plastid	87.98	83.3
AT2G36580.1	Thale cress	cytosol, peroxisome, plastid	87.78	83.11
Zm00001d052494_P001	Maize	plasma membrane	87.58	82.92
KRG96114	Soybean	cytosol	89.98	82.84
Zm00001d023379_P001	Maize	extracellular, plasma membrane	87.37	82.73
KXG22956	Sorghum	cytosol, peroxisome, plastid	87.37	82.73
GSMUA_Achr10P...	Banana	cytosol	86.77	82.16
KRH57945	Soybean	cytosol, endoplasmic reticulum	31.46	81.77
GSMUA_Achr6P13430_001	Banana	cytosol	84.97	80.15
PGSC0003DMT400070265	Potato	cytosol	82.97	78.56
GSMUA_Achr2P20210_001	Banana	plastid	85.17	77.84
KRH57981	Soybean	cytosol	40.48	66.67
PGSC0003DMT400070332	Potato	endoplasmic reticulum, extracellular, vacuole	18.24	48.66
PGSC0003DMT400028308	Potato	plastid	41.08	40.84
PGSC0003DMT400065094	Potato	cytosol	41.68	40.78
PGSC0003DMT400016844	Potato	plastid	25.45	21.97
PGSC0003DMT400062238	Potato	plastid	25.45	21.9
PGSC0003DMT400066112	Potato	plastid	25.05	21.74
TraesCS1D01G104600.1	Wheat	cytosol, extracellular, peroxisome	3.21	11.19
PGSC0003DMT400050451	Potato	plastid	15.83	10.45

Protein Annotations

KEGG:00010+2.7.1.40	KEGG:00230+2.7.1.40	KEGG:00620+2.7.1.40	Gene3D:2.40.33.10	Gene3D:3.20.20.60	Gene3D:3.40.1380.20
MapMan:50.2.7	GO:GO:0000287	GO:GO:0003674	GO:GO:0003824	GO:GO:0004743	GO:GO:0005488
GO:GO:0005975	GO:GO:0006091	GO:GO:0006096	GO:GO:0006139	GO:GO:0008150	GO:GO:0008152
GO:GO:0009056	GO:GO:0009058	GO:GO:0009987	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740
GO:GO:0030955	InterPro:IPR015806	InterPro:IPR036918	UniProt:M1CQ92	PFAM:PF00224	PFAM:PF02887
EnsemblPlantsGene:PGSC0003DMG400028177	PGSC:PGSC0003DMG400028177	EnsemblPlants:PGSC0003DMT400072411	PRINTS:PR01050	PANTHER:PTHR11817	PANTHER:PTHR11817:SF38
InterPro:Pyr_Knase	InterPro:Pyrv/PenolPyrv_Kinase-like_dom	InterPro:Pyrv_Knase-like_insert_dom_sf	InterPro:Pyrv_Knase_C	InterPro:Pyrv_Knase_C_sf	InterPro:Pyrv_Knase_brl
InterPro:Pyrv_Knase_insert_dom_sf	SUPFAM:SSF50800	SUPFAM:SSF51621	SUPFAM:SSF52935	TIGRFAMs:TIGR01064	UniParc:UPI000294C8DB

Description

Pyruvate kinase [Source:PGSC_GENE;Acc:PGSC0003DMG400028177]

Coordinates

chr10:-:55425687..55432306

Molecular Weight (calculated)

54159.4 Da

IEP (calculated)

7.089

GRAVY (calculated)

0.011

Length

499 amino acids

Sequence
(BLAST)

001: MTKIVGTLGP KSRSVEAISA CLKAGMSVAR FDFSWGDSEY HQETLENLKA SIKATKKLCA VMLDTVGAEL QVVNKRETTI SLTEDAIVTL TPHQGQEASN
101: EVLPINFGGL AKAVKKGDTI FVGQYLFTGS ETTSVWLEVD QVNGDDVICV VKNSATLAGS MFTLHASQVH IDMPTLTDKD KEVISTWGVQ NKIDFISLSY
201: TRHAEDVREA REFLSKLGDL SQTQIFAKIE NVEGLTHFDE ILKEADGIIL SRGNLGIDLP PEKVFLFQKA AVHKCNMAGK PAVVTRVVDS MTDNLRPTRA
301: EATDVANAVL DGTDAILLGA ETLRGLYPVE TISTVGKICA EAGKVFNQDL YFKKTVKFVG EPMTHLEAIA SSAVRAAIKV KASVIICFTS SGRAARLIAK
401: YRPTMPVLSV VIPRLKTNQL KWSFTGAFEA RQSLIVRGLF PMLADPRHPA ESNNATNESV LKVALDHGKA SGVIKSHDRV VVCQKVGDAS VVKIIELED

Best Arabidopsis Sequence Match ( AT3G52990.1 )
(BLAST)

001: MHSSHLLLEE PIRMASILEP SKSSFFPALT KIVGTLGPKS RSVEALSGCL KAGMSVARFD FSWGDADYHQ ETLDNLKVAV RSTKKLCAVM LDTVGPELQV
101: INKSEKAITL KADGLVTLTP NQDQEASSEV LPINFNGLAK AVKKGDTIFV GQYLFTGSET TSVWLEVDEV KGDDVICLSR NAATLAGSLF TLHSSQVHID
201: LPTLTEKDKE VISTWGVQNK IDFLSLSYCR HAEDVRQTRE MLKKLGDLSQ TQIFAKIENV EGLTHFDEIL QEADGIILSR GNLGIDLPPE KVFLFQKAAL
301: YKCNMAGKPA VLTRVVDSMT DNLRPTRAEA TDVANAVLDG SDAILLGAET LRGLYPVETI STVGRICAEA EKVFNQDLYF KKTVKYVGEP MTHLESIASS
401: AVRAAIKVKA SVIICFTSSG RAARLIAKYR PTMPVISVVI PRVKTNQLKW SFSGAFEARQ SLIVRGLFPM LADPRHPAES TSATNESVLK VALDHGKHAG
501: VIKSHDRVVV CQKVGDASVV KIIELED

Arabidopsis Description

Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q94KE3]
SUBAcon: [plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.