Factsheet for Bra030001.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 2
cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	nucleus
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

Inferred distinct locusB in Crop

locusB	locations
Bra000214.1-P
Bra002368.1-P
Bra006595.1-P
Bra018296.1-P
Bra033467.1-P
Bra039723.1-P

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G50310.1	Bra000214.1-P	AT2G41090.1	17360592
AT3G50310.1	Bra018296.1-P	AT3G51920.1	17360592
AT3G50310.1	Bra033467.1-P	AT3G51920.1	17360592
AT3G50310.1	Bra039723.1-P	AT4G14640.1	17360592
AT3G50310.1	Bra002368.1-P	AT5G21274.1	17360592
AT3G50310.1	Bra006595.1-P	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDX83120	Canola	cytosol	99.7	99.7
Bra036070.1-P	Field mustard	cytosol	82.89	82.16
AT3G50310.1	Thale cress	cytosol	75.22	74.56
Bra012131.1-P	Field mustard	cytosol	71.68	70.23
Bra011739.1-P	Field mustard	plastid	56.64	54.24
KRH74789	Soybean	nucleus	50.74	52.44
KRH28462	Soybean	plastid	53.39	52.31
KRH28449	Soybean	plastid	40.71	52.08
KRH77009	Soybean	nucleus	53.1	52.02
KRH66431	Soybean	plastid	53.1	51.72
PGSC0003DMT400065492	Potato	nucleus	51.33	48.88
KRH04602	Soybean	nucleus	48.38	48.09
Solyc02g064980.1.1	Tomato	cytosol	49.85	47.08
Solyc02g090970.1.1	Tomato	cytosol, nucleus, plasma membrane, plastid	49.56	46.67
PGSC0003DMT400010341	Potato	cytosol	48.67	46.35
KRH57939	Soybean	nucleus	46.61	46.33
PGSC0003DMT400010342	Potato	cytosol, plastid	48.97	46.11
Solyc02g090990.1.1	Tomato	cytosol	47.49	45.22
PGSC0003DMT400010340	Potato	nucleus	47.79	45.0
VIT_07s0031g00530.t01	Wine grape	mitochondrion	48.97	44.99
Solyc02g090980.1.1	Tomato	cytosol	46.9	44.79
KRH66433	Soybean	cytosol, plastid	43.36	42.49
GSMUA_Achr9P21940_001	Banana	cytosol, mitochondrion, peroxisome, plastid	44.84	38.29
Bra022773.1-P	Field mustard	plastid	20.35	35.94
Bra039693.1-P	Field mustard	cytosol	28.91	35.77
Bra015388.1-P	Field mustard	cytosol, plasma membrane, plastid	35.4	35.61
GSMUA_Achr4P13370_001	Banana	cytosol	48.97	35.1
Bra025496.1-P	Field mustard	cytosol	18.88	32.32
Bra021794.1-P	Field mustard	mitochondrion	33.92	32.21
Bra005599.1-P	Field mustard	mitochondrion	34.51	31.88
Bra018010.1-P	Field mustard	mitochondrion	30.09	31.1
Bra018220.1-P	Field mustard	nucleus	34.81	28.78
Bra026395.1-P	Field mustard	plastid	38.35	28.38
Bra035552.1-P	Field mustard	cytosol	35.99	28.31
Bra002933.1-P	Field mustard	cytosol	36.58	28.12
Bra028991.1-P	Field mustard	cytosol	35.4	26.61
Bra010423.1-P	Field mustard	mitochondrion, plastid	36.28	26.06
Bra013996.1-P	Field mustard	mitochondrion	28.91	25.26
Bra002635.1-P	Field mustard	plastid	35.99	24.85
Bra031828.1-P	Field mustard	nucleus, plasma membrane	16.52	21.29
Bra018344.1-P	Field mustard	cytosol	18.29	18.56
Bra000647.1-P	Field mustard	cytosol	29.5	18.48
Bra037860.1-P	Field mustard	cytosol	29.79	16.86
Bra020785.1-P	Field mustard	cytosol	28.61	14.88
Bra013139.1-P	Field mustard	nucleus	25.96	14.86
Bra018588.1-P	Field mustard	nucleus	27.73	14.55
Bra012110.1-P	Field mustard	nucleus	29.5	14.27
Bra027792.1-P	Field mustard	nucleus	29.5	11.39
Bra039396.1-P	Field mustard	cytosol	21.83	7.11
Bra001280.1-P	Field mustard	cytosol	26.84	6.98
Bra001510.1-P	Field mustard	cytosol	26.84	6.72
Bra027424.1-P	Field mustard	extracellular	1.47	2.75

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.2.2	EnsemblPlantsGene:Bra030001	EnsemblPlants:Bra030001.1	EnsemblPlants:Bra030001.1-P	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488	GO:GO:0005524
GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:Kinase-like_dom_sf	UniProt:M4EMI1	PFAM:PF00069
PIRSF:PIRSF000615	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24361	PANTHER:PTHR24361:SF334
InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0002544518

Description

AT3G50310 (E=2e-143) MAPKKK20 | MAPKKK20; ATP binding / kinase/ protein kinase/ protein serine/threonine kinase

Coordinates

chrA01:-:15614544..15615563

Molecular Weight (calculated)

37420.2 Da

IEP (calculated)

4.368

GRAVY (calculated)

-0.213

Length

339 amino acids

Sequence
(BLAST)

001: MEWVRGETIG YGTFSTVSLA TPSDSGGFPP LMAVKSADSY GATSLVNEKS VLDSLGDCPE IVRCYGEDQT VEKGEKLHNL LLEYAWRGSL ATQLMKLNGE
101: GLPESTVRRH TGSLLRGLRH IHSRGFAHCD IKLGNILLFD DGAVKIADFG LARDLTVSRE GVEIRGTPLY MAPESVNDNV YGTAADVWAL GCAVVEMSSG
201: KTAWSVEEGL NFMSLLIRIG VGDELPKVPE DLSEEGKDFL SKCFVKDPEE RWTAEMLLNH PFVAVDCEDN GQREDLFVEV KEKVSTSPRC PFEFSDWEPI
301: ASESVMQYQP LDSPVERFES LVSESIPNWS IAQGWVTVR

Best Arabidopsis Sequence Match ( AT3G50310.1 )
(BLAST)

001: MEWVRGETIG FGTFSTVSTA TKSRNSGDFP ALIAVKSTDA YGAASLSNEK SVLDSLGDCP EIIRCYGEDS TVENGEEMHN LLLEYASRGS LASYMKKLGG
101: EGLPESTVRR HTGSVLRGLR HIHAKGFAHC DIKLANILLF NDGSVKIADF GLAMRVDGDL TALRKSVEIR GTPLYMAPEC VNDNEYGSAA DVWALGCAVV
201: EMFSGKTAWS VKEGSHFMSL LIRIGVGDEL PKIPEMLSEE GKDFLSKCFV KDPAKRWTAE MLLNHSFVTI DLEDDHRENF VVKVKDEDKV LMSPKCPFEF
301: DDWDSFTLDS NPSFDSPVER LGSLVSGSIP DWSVGGSWLT VR

Arabidopsis Description

MAPKKK20MKKK20 [Source:UniProtKB/TrEMBL;Acc:A0A178VGW0]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.