CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

nucleus 2
cytosol 1
mitochondrion 1
extracellular 5
endoplasmic reticulum 3
vacuole 3
plasma membrane 4
golgi 3
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
iPSORT:	mitochondrion
MultiLoc:	extracellular
Plant-mPloc:	extracellular, nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plasma membrane

nucleus: 21132161
extracellular: 25724908
endoplasmic reticulum: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 25724908 doi

VC Clarke, PC Loughlin, A Gavrin, C Chen, EM Brear, DA Day, PM Smith

From the ‡University of Sydney, School of Biological Sciences, Sydney Australia;, From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; penny.smith@sydney.edu.au., From the ‡University of Sydney, School of Biological Sciences, Sydney Australia; §Flinders University, School of Biological Sciences, Adelaide Australia.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH42483
KRH58580

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G13750.1	KRH42483	AT2G01950.1	19000166
AT1G13750.1	KRH58580	AT2G01950.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH45750	Soybean	vacuole	94.64	94.64
Zm00001d025796_P001	Maize	nucleus	20.29	76.22
Zm00001d035906_P001	Maize	cytosol	15.1	75.0
AT1G13750.1	Thale cress	extracellular, plasma membrane, vacuole	72.4	72.76
KRH70903	Soybean	endoplasmic reticulum, extracellular, golgi, plastid	75.0	72.07
GSMUA_Achr3P15950_001	Banana	vacuole	70.78	71.13
CDY16105	Canola	extracellular	70.78	70.89
HORVU5Hr1G020400.5	Barley	extracellular	70.29	70.87
Bra026890.1-P	Field mustard	extracellular, golgi	71.43	70.85
CDY33806	Canola	extracellular, golgi, vacuole	71.43	70.85
TraesCS5A01G082400.1	Wheat	plastid	70.13	70.82
Os12t0576700-01	Rice	extracellular, golgi, vacuole	69.97	70.54
Os12t0576600-01	Rice	extracellular, vacuole	69.64	70.33
TraesCS5B01G083000.1	Wheat	endoplasmic reticulum, extracellular	69.64	70.21
EES16213	Sorghum	extracellular	69.97	69.63
GSMUA_Achr4P32800_001	Banana	extracellular, vacuole	69.16	69.61
Os03t0214000-01	Rice	extracellular, plasma membrane	69.32	69.43
EER95277	Sorghum	extracellular	69.64	69.42
TraesCS5B01G082700.1	Wheat	endoplasmic reticulum, extracellular, golgi	69.64	69.31
TraesCS5B01G083600.1	Wheat	endoplasmic reticulum, extracellular, golgi	69.32	68.98
KXG23786	Sorghum	extracellular	67.86	68.3
TraesCS5B01G083700.1	Wheat	extracellular	70.13	68.14
KRH57229	Soybean	endoplasmic reticulum	68.99	68.11
TraesCS4B01G247300.1	Wheat	extracellular	68.18	68.07
TraesCS4D01G246600.1	Wheat	golgi	67.05	67.82
Zm00001d048090_P002	Maize	plasma membrane	69.64	67.77
KRH54186	Soybean	endoplasmic reticulum	68.18	67.42
CDX98126	Canola	extracellular, plasma membrane	67.69	67.37
Bra019705.1-P	Field mustard	extracellular, plasma membrane	67.21	66.77
Os12t0576750-01	Rice	extracellular, golgi, vacuole	66.56	66.45
KRH54187	Soybean	nucleus	31.82	66.44
Zm00001d030765_P003	Maize	extracellular	69.48	66.15
Zm00001d008182_P001	Maize	mitochondrion	18.67	66.09
Zm00001d028865_P001	Maize	mitochondrion	18.67	66.09
Zm00001d051353_P001	Maize	mitochondrion	18.67	66.09
Zm00001d050994_P001	Maize	cytosol	8.77	65.85
CDY36408	Canola	extracellular, plasma membrane	56.66	65.6
Zm00001d035068_P001	Maize	mitochondrion	18.51	65.52
Zm00001d012990_P001	Maize	mitochondrion	18.51	65.52
TraesCS4A01G057700.2	Wheat	extracellular	67.69	64.85
TraesCS5D01G090100.3	Wheat	plastid	69.97	64.62
Zm00001d006374_P001	Maize	extracellular	68.67	63.99
KXG36229	Sorghum	plastid	69.16	63.77
HORVU4Hr1G067950.8	Barley	extracellular, plasma membrane	68.02	63.29
TraesCS5A01G076900.1	Wheat	extracellular, golgi, vacuole	69.48	62.94
KRH63765	Soybean	mitochondrion	38.64	45.86
KRH39860	Soybean	vacuole	32.79	31.56
KRH23936	Soybean	extracellular	32.47	30.35
KRG89498	Soybean	extracellular, plasma membrane	29.71	27.64
KRG96186	Soybean	extracellular, golgi, vacuole	18.83	27.04
KRH67898	Soybean	extracellular, golgi, vacuole	18.67	26.74
KRH51772	Soybean	extracellular, golgi, vacuole	18.83	24.47
KRH73723	Soybean	extracellular	18.02	24.45
KRH20185	Soybean	nucleus	18.83	24.42
KRH14423	Soybean	extracellular, golgi	18.02	24.13
KRH51771	Soybean	cytosol	17.37	24.1
KRH23858	Soybean	extracellular	18.02	23.92
KRH46137	Soybean	extracellular, plastid, vacuole	18.18	23.88
KRH27195	Soybean	extracellular, plasma membrane, vacuole	18.18	23.88
KRH32727	Soybean	endoplasmic reticulum	16.23	23.75
KRH39937	Soybean	extracellular, vacuole	17.86	23.71
KRH31582	Soybean	extracellular, mitochondrion, peroxisome, plastid	17.69	23.39
KRH30846	Soybean	cytosol	6.98	23.24
KRG96185	Soybean	plastid	18.34	23.06
KRH22146	Soybean	extracellular, golgi	17.53	22.93
KRH03620	Soybean	endoplasmic reticulum	18.67	22.46
KRH67897	Soybean	plastid	17.86	22.4
KRH18439	Soybean	extracellular	7.63	22.17
KRH46686	Soybean	extracellular	19.48	21.94
KRG97331	Soybean	extracellular	19.97	21.5
KRG93588	Soybean	extracellular	19.64	20.61

Protein Annotations

EntrezGene:100817130	Gene3D:3.60.21.10	MapMan:35.1	EMBL:ACUP02011013	InterPro:Calcineurin-like_PHP_ApaH	EnsemblPlantsGene:GLYMA_18G132500
GO:GO:0003674	GO:GO:0003824	GO:GO:0003993	GO:GO:0005488	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016311	GO:GO:0016787	GO:GO:0046872	InterPro:IPR008963	InterPro:IPR029052
UniProt:K7MRU1	EnsemblPlants:KRG99244	ProteinID:KRG99244	ProteinID:KRG99244.1	InterPro:Metallo-depent_PP-like	PFAM:PF00149
PFAM:PF14008	PFAM:PF16656	PANTHER:PTHR22953	PANTHER:PTHR22953:SF20	InterPro:Purple_acid_PPase_C_dom	InterPro:Purple_acid_Pase-like_N
InterPro:Purple_acid_Pase_N	SUPFAM:SSF49363	SUPFAM:SSF56300	SignalP:SignalP-noTM	UniParc:UPI0002966B94	SEG:seg

Description

hypothetical protein

Coordinates

chr18:+:18646384..18652078

Molecular Weight (calculated)

69379.7 Da

IEP (calculated)

6.572

GRAVY (calculated)

-0.373

Length

616 amino acids

Sequence
(BLAST)

001: MMTNGMGKSR VLFFSMLVLA TFQKAVSEEH QPLSKVAIHK TTLALDERAF IKATPNVLGL KGQNTEWVTL QYSNPKPTVD DWIGVFSPAN FNASTCPAEN
101: IWVNPPFLCS APIKYQYANF SSHGYKNTGK GSLKLQLINQ RSDFSFALFT GGLTNPKLVA VSNKVSFINP NAPVYPRLAQ GKTWDEMTVT WTSGYEISDA
201: EPFVEWGPKG GNLVKSPAGT LTFDRNTMCG APARTVGWRD PGYIHTSFLK ELWPNREYKY KLGHKLFNGT IIWSQEYQFK ASPYPGQNSL QRVVIFGDMG
301: KAEADGSNEY NNFQPGSLNT TKQIIQDLKD IDIVFNIGDL SYANGYLSQW DQFTAQIEPI ASTVPYMTAS GNHERDWPDT GSFYGNLDSG GECGVLAQTM
401: FYVPAENREK FWYSVDYGMF RFCIANTELD WRKGSEQYKF IENCLASVDR QKQPWLIFLA HRVLGYSSAG FYVAEGSFEE PMGREDLQYL WQKYKVDIAM
501: YGHVHNYERT CPVYQNICTN KEEHNYKGSL DGTIHVVVGG GGASLAEFAP INTTWSIFKD HDFGFVKLTA FDHSNLLFEY KKSSDGQVYD SFKISRQYRD
601: ILACTVDSCP PTTLAS

Best Arabidopsis Sequence Match ( AT1G13750.1 )
(BLAST)

001: MRESLVAILV TVISVLGAIH QVKSHEDQPL SGIAVHKITF GLNEKAYVKA SPTVLGSNGQ HSELVLVQYS SPKPSDDDWI GVFSPADFNA STCPGDNKMV
101: QPPRLCSAPV KFQYANFSNP RYTNTGTGSL KLQLINQRSD FSFALFSGGL LNPKLVAISN KVAFENPNAP VYPRLALGKE WDEMTVTWTS GYGLNLAEPV
201: VEWGVKGGER KLSPAGTLTF ARNSMCGAPA RTVGWRDPGY IHTAFLKELW PNSKYTYRVG HRLSNGALIW SKEYQFKSSP FPGQNSVQQV VIFGDMGKAE
301: VDGSSEYNDF QRASLNTTKQ LIKDLKKTDA VFHIGDICYA NGYLSQWDQF IAQIEPIAST VPYMIASGNH ERVWPNSGSF YEGLDSGGEC GVPAETMFYV
401: PAQNRAKVWY SSDYGMFRFC VADTEHDWRE GTEQYNFIEH CLASVDRQKQ PWLIFLAHRV LGYSSTYFYA EEGSFAEPMG RESLQKLWQK YKVDIAIYGH
501: AHNYERTCPV YQSVCTSHEK SNYKAPLNGT IHIVAGGGGA GLAEFSDLQP NWSLFRDYDY GFLKLTAIDH SNLLFEYKKS SDGRVHDSFT ISKDYRDILA
601: CAVDSCPATT LAS

Arabidopsis Description

PAP1Probable inactive purple acid phosphatase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9LMX4]
SUBAcon: [plasma membrane,extracellular,vacuole]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.