Factsheet for Zm00001d045675_P003

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

plastid 2
cytosol 3
mitochondrion 1
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular
Any Predictor:	cytosol, mitochondrion, plastid, secretory
iPSORT:	plastid
MultiLoc:	cytosol
Predotar:	secretory
PProwler:	mitochondrion
TargetP:	plastid
WoLF PSORT:	cytosol
YLoc:	cytosol

extracellular: 20408568

msms PMID: 20408568 doi

W Ma, N Muthreich, C Liao, M Franz-Wachtel, W Schütz, F Zhang, F Hochholdinger, C Li

Department of Plant Nutrition, China Agricultural University, Beijing, PR China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER89574	Sorghum	cytosol	96.79	96.79
Os06t0265000-01	Rice	cytosol	90.19	90.19
TraesCS1B01G408200.1	Wheat	cytosol, plastid	86.97	87.56
TraesCS1D01G430300.1	Wheat	cytosol, mitochondrion	78.34	87.52
TraesCS1A01G382800.1	Wheat	cytosol, mitochondrion	86.29	86.88
TraesCS1B01G453600.1	Wheat	cytosol, plastid	86.46	86.76
TraesCS1D01G390500.1	Wheat	cytosol, plastid	86.63	86.63
TraesCS1A01G422100.1	Wheat	cytosol, mitochondrion	85.79	86.08
Solyc04g055200.2.1	Tomato	cytosol	57.53	85.21
CDY14529	Canola	cytosol	79.53	83.19
CDY08829	Canola	cytosol	79.53	83.19
Bra024345.1-P	Field mustard	cytosol	79.53	83.19
KRH31291	Soybean	cytosol, nucleus	79.53	83.04
KRG97578	Soybean	cytosol	79.53	82.6
AT5G65010.2	Thale cress	cytosol	80.54	82.21
AT5G10240.1	Thale cress	cytosol	80.37	82.18
Bra006053.1-P	Field mustard	mitochondrion, plastid	79.19	80.69
HORVU1Hr1G092110.4	Barley	cytosol, plastid	86.8	80.66
CDX81165	Canola	plastid	79.02	80.52
CDX78372	Canola	plastid	79.02	80.52
GSMUA_Achr9P15390_001	Banana	cytosol, mitochondrion	84.43	78.83
Zm00001d031563_P001	Maize	cytosol	23.86	78.33
HORVU1Hr1G084370.2	Barley	plastid	86.63	77.93
Zm00001d010355_P001	Maize	mitochondrion	28.93	76.34
Zm00001d044608_P001	Maize	cytosol	73.1	71.29
Zm00001d028750_P002	Maize	extracellular	69.04	69.39
Zm00001d028766_P001	Maize	endoplasmic reticulum	35.19	68.2
Zm00001d047736_P001	Maize	cytosol	61.93	64.32
CDY23169	Canola	mitochondrion	9.48	59.57
CDX87183	Canola	cytosol	12.18	51.43
CDY54341	Canola	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	11.84	50.0
CDY50475	Canola	cytosol	11.84	50.0
CDY01568	Canola	cytosol	11.84	50.0
CDY01480	Canola	cytosol	11.84	50.0
CDY17904	Canola	cytosol	11.68	48.59
CDX86918	Canola	cytosol, mitochondrion	8.63	38.06
Zm00001d010196_P002	Maize	plastid	13.03	31.17

Protein Annotations

KEGG:00250+6.3.5.4	Gene3D:3.40.50.620	Gene3D:3.60.20.10	MapMan:4.1.2.1.1	EntrezGene:542435	ProteinID:AQL02965.1
InterPro:AsnB_N	InterPro:Asn_synth_AEB	InterPro:Asn_synthase	UniProt:B4FFJ0	EMBL:BT035878	EMBL:FM211451
EMBL:FM212633	InterPro:GATase_2_dom	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004066
GO:GO:0005488	GO:GO:0005524	GO:GO:0006529	GO:GO:0006541	GO:GO:0008150	GO:GO:0008152
GO:GO:0008652	GO:GO:0009058	GO:GO:0009987	GO:GO:0016874	GO:GO:0070981	InterPro:IPR014729
InterPro:IPR017932	InterPro:IPR029055	InterPro:Ntn_hydrolases_N	PFAM:PF00733	PFAM:PF13537	PIRSF:PIRSF001589
PFscan:PS51278	PANTHER:PTHR11772	PANTHER:PTHR11772:SF13	InterPro:Rossmann-like_a/b/a_fold	SUPFAM:SSF52402	SUPFAM:SSF56235
UniParc:UPI00017B70D2	EnsemblPlantsGene:Zm00001d045675	EnsemblPlants:Zm00001d045675_P003	EnsemblPlants:Zm00001d045675_T003	SEG:seg	:

Description

asparagine synthetase1 asparagine synthetase1

Coordinates

chr9:-:32681305..32690688

Molecular Weight (calculated)

66324.9 Da

IEP (calculated)

6.317

GRAVY (calculated)

-0.293

Length

591 amino acids

Sequence
(BLAST)

001: MCGILAVLGV AEVSLAKRSR IIELSRRLRH RGPDWSGLHC HEDCYLAHQR LAIIDPTSGD QPLYNEDKTV VVTVNGEIYN HEELKAKLKT HEFQTGSDCE
101: VIAHLYEEYG EEFVDMLDGM FSFVLLDTRD KSFIAARDAI GICPLYMGWG LDGSVWFSSE MKALSDDCER FITFPPGHLY SSKTGGLRRW YNPPWFSETV
201: PSTPYNALFL REMFEKAVIK RLMTDVPFGV LLSGGLDSSL VASVASRHLN ETKVDRQWGN KLHTFCIGLK GSPDLKAARE VADYLSTVHH EFHFTVQEGI
301: DALEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVI SGEGSDEIFG GYLYFHKAPN KKEFHEETCR KIKALHLYDC LRANKATSAW GVEARVPFLD
401: KSFISVAMDI DPEWKMIKRD LGRIEKWVIR NAFDDDERPY LPKHILYRQK EQFSDGVGYS WIDGLKDHAS QHVSDSMMMN AGFVYPENTP TTKEGYYYRM
501: IFEKFFPKPA ARSTVPGGPS VACSTAKAVE WDASWSKNLD PSGRAALGVH DAAYEDTAGK TPASADPVSD KGLRPAIGES LGTPVASATA V

Best Arabidopsis Sequence Match ( AT5G65010.1 )
(BLAST)

001: MCGILAVLGC IDNSQAKRSR IIELSRRLRH RGPDWSGLHC YEDCYLAHER LAIIDPTSGD QPLYNEDKTV AVTVNGEIYN HKILREKLKS HQFRTGSDCE
101: VIAHLYEEHG EEFIDMLDGM FAFVLLDTRD KSFIAARDAI GITPLYIGWG LDGSVWFASE MKALSDDCEQ FMSFPPGHIY SSKQGGLRRW YNPPWYNEQV
201: PSTPYDPLVL RNAFEKAVIK RLMTDVPFGV LLSGGLDSSL VAAVALRHLE KSEAARQWGS QLHTFCIGLQ GSPDLKAGRE VADYLGTRHH EFQFTVQDGI
301: DAIEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEILG GYLYFHKAPN KKEFHEETCR KIKALHQFDC LRANKSTSAW GVEARVPFLD
401: KEFLNVAMSI DPEWKLIKPD LGRIEKWVLR NAFDDEERPY LPKHILYRQK EQFSDGVGYS WIDGLKDHAN KHVSDTMLSN ASFVFPDNTP LTKEAYYYRT
501: IFEKFFPKSA ARATVPGGPS IACSTAKAVE WDATWSKNLD PSGRAALGVH VAAYEEDKAA AAAKAGSDLV DPLPKNGT

Arabidopsis Description

ASN2asparagine synthetase 2 [Source:TAIR;Acc:AT5G65010]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.