CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: extracellular

Predictor Summary:

nucleus 1
extracellular 6
endoplasmic reticulum 4
vacuole 4
plasma membrane 4
golgi 4
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	nucleus
iPSORT:	secretory
MultiLoc:	extracellular
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	extracellular

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY48195	Canola	vacuole	93.28	93.75
AT5G45910.1	Thale cress	extracellular	75.37	81.45
KRH10999	Soybean	extracellular	50.99	54.81
Solyc03g006240.2.1	Tomato	nucleus	49.01	53.53
PGSC0003DMT400011187	Potato	plasma membrane	49.25	53.23
KRH21298	Soybean	nucleus	50.5	51.79
TraesCS3B01G163300.1	Wheat	extracellular	45.52	49.73
TraesCS3A01G141300.1	Wheat	extracellular	45.52	49.46
TraesCS3D01G145800.1	Wheat	extracellular	45.02	48.92
HORVU3Hr1G028000.2	Barley	extracellular	46.02	48.56
EES02257	Sorghum	endoplasmic reticulum	45.77	48.55
Os01t0215700-01	Rice	endoplasmic reticulum, extracellular, golgi, mitochondrion, plasma membrane, vacuole	45.02	47.88
GSMUA_Achr10P...	Banana	extracellular, vacuole	42.29	45.95
GSMUA_Achr8P19960_001	Banana	extracellular	39.55	44.92
GSMUA_Achr8P19970_001	Banana	extracellular, vacuole	39.8	44.2
Os05t0209600-01	Rice	extracellular, plasma membrane	39.3	43.77
GSMUA_Achr4P31080_001	Banana	vacuole	38.81	43.58
CDX90163	Canola	extracellular, plasma membrane, vacuole	42.04	43.33
CDY43309	Canola	extracellular, plasma membrane, vacuole	41.54	42.82
KRH32795	Soybean	extracellular	38.31	42.78
CDX90162	Canola	extracellular	41.29	42.56
CDY63997	Canola	plastid	36.82	42.53
EES17878	Sorghum	extracellular	38.31	42.31
Os01t0214800-01	Rice	golgi	39.05	42.2
HORVU0Hr1G031170.3	Barley	extracellular, plasma membrane, vacuole	37.81	41.99
HORVU1Hr1G066090.2	Barley	extracellular, plasma membrane, vacuole	37.81	41.99
CDY15655	Canola	extracellular	41.04	41.98
CDX94634	Canola	plastid	41.54	41.96
KRH63839	Soybean	vacuole	38.81	41.94
TraesCS6B01G041400.1	Wheat	extracellular, vacuole	31.34	41.86
Os01t0214600-01	Rice	extracellular	36.32	41.83
KXG21541	Sorghum	extracellular	38.06	41.8
KRG93967	Soybean	vacuole	37.31	41.78
TraesCS5B01G209000.1	Wheat	extracellular	38.56	41.78
TraesCS7B01G235100.1	Wheat	extracellular, vacuole	38.31	41.73
TraesCS7D01G330100.1	Wheat	extracellular, vacuole	38.31	41.73
GSMUA_Achr10P...	Banana	extracellular, golgi	37.31	41.67
CDY63998	Canola	extracellular, vacuole	40.3	41.65
CDY64716	Canola	extracellular, plasma membrane	40.3	41.65
EES00053	Sorghum	extracellular	39.05	41.53
TraesCS1D01G321300.1	Wheat	endoplasmic reticulum, golgi, plasma membrane	37.56	41.48
TraesCS1B01G333800.1	Wheat	extracellular, plasma membrane, vacuole	37.31	41.44
EES02259	Sorghum	plasma membrane	39.05	41.42
CDY43308	Canola	extracellular	41.04	41.35
TraesCS3B01G163400.1	Wheat	extracellular, vacuole	37.31	41.32
TraesCSU01G177500.2	Wheat	endoplasmic reticulum, extracellular, vacuole	39.55	41.3
TraesCSU01G219900.1	Wheat	endoplasmic reticulum, extracellular, vacuole	39.55	41.3
TraesCS1A01G321100.1	Wheat	endoplasmic reticulum, golgi, plasma membrane	37.31	41.21
CDY34089	Canola	extracellular	39.8	41.13
TraesCS2B01G158900.1	Wheat	cytosol	33.33	41.1
EER89867	Sorghum	extracellular	38.06	40.91
KXG21542	Sorghum	extracellular	33.83	40.84
EES00052	Sorghum	golgi	37.56	40.81
TraesCS3D01G145900.1	Wheat	extracellular	36.82	40.66
TraesCS3D01G146200.1	Wheat	golgi	37.56	40.59
TraesCS7A01G348900.1	Wheat	extracellular, golgi	37.56	40.59
CDY34093	Canola	mitochondrion, plasma membrane	40.05	40.55
Os06t0531600-01	Rice	mitochondrion	37.81	40.53
HORVU5Hr1G061880.5	Barley	extracellular, vacuole	38.31	40.53
TraesCS5B01G211600.1	Wheat	extracellular	37.06	40.49
TraesCS7B01G233800.1	Wheat	extracellular, golgi	37.56	40.48
TraesCS3B01G174900.1	Wheat	extracellular, golgi	38.06	40.48
Os01t0215500-01	Rice	extracellular	30.6	40.46
CDX94632	Canola	extracellular, plasma membrane, vacuole	38.31	40.31
CDY30819	Canola	mitochondrion, plasma membrane	39.8	40.3
Zm00001d036237_P002	Maize	extracellular	36.32	40.22
TraesCS5D01G221700.1	Wheat	extracellular	36.82	40.22
TraesCS7D01G329700.1	Wheat	extracellular, golgi, mitochondrion	37.31	40.21
TraesCS3B01G175000.1	Wheat	extracellular, golgi	37.81	40.21
Os03t0365900-01	Rice	cytosol	22.89	40.17
Zm00001d008630_P001	Maize	extracellular	35.07	40.17
TraesCS7D01G108900.1	Wheat	endoplasmic reticulum, plasma membrane, vacuole	36.07	40.17
EES02254	Sorghum	extracellular	37.56	40.05
TraesCS7B01G013200.1	Wheat	cytosol, plastid	25.62	39.92
CDY30822	Canola	extracellular, plasma membrane	39.8	39.9
TraesCS3A01G141100.1	Wheat	extracellular, golgi, plasma membrane	37.31	39.89
TraesCS7A01G113600.1	Wheat	endoplasmic reticulum, plasma membrane, vacuole	35.82	39.89
TraesCSU01G095400.1	Wheat	endoplasmic reticulum, plasma membrane, vacuole	35.82	39.89
TraesCSU01G169100.1	Wheat	extracellular, golgi	38.56	39.85
TraesCS3A01G141400.1	Wheat	extracellular	37.56	39.84
TraesCS3A01G141200.1	Wheat	extracellular	36.07	39.84
TraesCSU01G173600.1	Wheat	extracellular	30.1	39.8
KXG19336	Sorghum	extracellular, plasma membrane	35.82	39.78
TraesCS1D01G005500.1	Wheat	endoplasmic reticulum, extracellular, vacuole	39.55	39.75
Zm00001d039326_P001	Maize	extracellular, plastid, vacuole	38.56	39.74
TraesCS3B01G163200.1	Wheat	endoplasmic reticulum, vacuole	37.56	39.74
Os06t0156600-01	Rice	plasma membrane	35.57	39.72
TraesCS5A01G287100.1	Wheat	extracellular, golgi, vacuole	35.07	39.72
TraesCS6A01G003000.1	Wheat	extracellular, vacuole	38.31	39.69
CDY15658	Canola	extracellular, golgi	38.06	39.64
TraesCS3B01G163700.2	Wheat	extracellular, golgi, vacuole	37.56	39.63
TraesCS3B01G175100.1	Wheat	extracellular, golgi	37.56	39.63
TraesCS3B01G175200.1	Wheat	extracellular, golgi	37.56	39.63
GSMUA_Achr8P19980_001	Banana	extracellular, plasma membrane	35.32	39.55
TraesCS6B01G220200.1	Wheat	extracellular, plasma membrane	37.56	39.53
TraesCS2D01G137700.1	Wheat	extracellular, vacuole	36.57	39.52
TraesCS6B01G041300.1	Wheat	vacuole	37.31	39.47
Zm00001d010450_P001	Maize	plastid	29.85	39.47
Os01t0216500-00	Rice	extracellular	37.06	39.42
HORVU2Hr1G003720.1	Barley	golgi	37.81	39.38
TraesCS1D01G127400.1	Wheat	cytosol, golgi, plastid	30.85	39.37
TraesCS2D01G025800.1	Wheat	extracellular, golgi, plastid	38.06	39.33
TraesCS7A01G113800.1	Wheat	extracellular, golgi, plasma membrane	37.06	39.31
TraesCS2A01G024600.2	Wheat	extracellular, vacuole	38.81	39.29
GSMUA_Achr10P...	Banana	plasma membrane	35.07	39.28
Zm00001d010451_P001	Maize	extracellular	32.34	39.27
TraesCS6D01G183800.1	Wheat	extracellular, plasma membrane	37.31	39.27
Zm00001d053425_P001	Maize	plasma membrane	37.31	39.27
TraesCS2A01G135200.1	Wheat	vacuole	36.32	39.25
Os01t0216300-01	Rice	endoplasmic reticulum, extracellular, plasma membrane	37.56	39.12
EES04851	Sorghum	extracellular	37.06	39.11
HORVU7Hr1G011810.1	Barley	extracellular, plasma membrane, vacuole	37.06	39.11
TraesCS4A01G426300.1	Wheat	extracellular	37.06	39.11
TraesCS4A01G426700.1	Wheat	extracellular	37.06	39.11
TraesCS7A01G062600.1	Wheat	extracellular, plasma membrane, vacuole	37.06	39.11
TraesCS7B01G011500.1	Wheat	plasma membrane	37.06	39.11
EER89868	Sorghum	golgi, mitochondrion, plastid	38.81	39.1
Zm00001d008929_P002	Maize	endoplasmic reticulum, extracellular, vacuole	38.31	39.09
TraesCS2B01G034800.1	Wheat	extracellular, golgi, plastid	37.81	39.07
HORVU2Hr1G023750.2	Barley	extracellular, vacuole	36.82	39.05
Os01t0223200-01	Rice	endoplasmic reticulum, extracellular, vacuole	36.82	39.05
TraesCS7D01G109100.1	Wheat	plasma membrane	36.82	39.05
TraesCS2A01G135300.1	Wheat	extracellular, vacuole	36.32	39.04
Os06t0531900-01	Rice	golgi	38.31	38.99
HORVU2Hr1G023770.2	Barley	extracellular, golgi	36.07	38.87
EES02253	Sorghum	endoplasmic reticulum, extracellular	36.82	38.85
TraesCS2B01G159000.1	Wheat	golgi, plasma membrane	29.35	38.82
TraesCS2D01G137500.2	Wheat	mitochondrion, vacuole	35.82	38.81
TraesCS3D01G146300.1	Wheat	plasma membrane	35.32	38.8
CDY15657	Canola	extracellular, plasma membrane	38.31	38.79
EES17881	Sorghum	endoplasmic reticulum	38.06	38.73
Zm00001d037025_P001	Maize	mitochondrion	37.56	38.72
TraesCS2B01G159200.1	Wheat	extracellular, vacuole	35.82	38.71
TraesCS3A01G258100.2	Wheat	extracellular, golgi	36.57	38.68
Os05t0210100-01	Rice	golgi	35.57	38.65
Zm00001d008928_P001	Maize	extracellular, vacuole	28.36	38.64
TraesCS3A01G148500.1	Wheat	extracellular, golgi	36.82	38.64
CDX94633	Canola	extracellular	38.06	38.64
TraesCS2D01G137600.1	Wheat	extracellular, plasma membrane, vacuole	35.32	38.59
Zm00001d016130_P001	Maize	extracellular	29.35	38.56
TraesCSU01G215000.1	Wheat	extracellular, vacuole	35.82	38.5
TraesCS5A01G422800.1	Wheat	extracellular	36.57	38.48
HORVU3Hr1G028020.4	Barley	endoplasmic reticulum, plasma membrane	36.82	38.44
EES02260	Sorghum	golgi, vacuole	35.07	38.42
TraesCS3D01G156000.1	Wheat	extracellular, golgi, vacuole	36.57	38.28
TraesCS5B01G424700.1	Wheat	endoplasmic reticulum, extracellular, vacuole	36.32	38.22
TraesCS3A01G140900.1	Wheat	golgi	35.32	38.17
EER89228	Sorghum	extracellular, golgi	34.83	38.15
TraesCS7D01G111800.1	Wheat	extracellular, golgi	34.83	38.04
Zm00001d036238_P001	Maize	plasma membrane	35.82	37.99
TraesCS3A01G140800.1	Wheat	plasma membrane	34.58	37.98
TraesCS5D01G217100.1	Wheat	extracellular, plasma membrane, vacuole	35.32	37.97
KRH54432	Soybean	extracellular, plasma membrane, vacuole	27.86	37.97
TraesCS5D01G431000.1	Wheat	endoplasmic reticulum, extracellular, vacuole	36.07	37.96
GSMUA_Achr7P14030_001	Banana	cytosol, plasma membrane	36.32	37.92
TraesCS2A01G135000.1	Wheat	endoplasmic reticulum, vacuole	35.32	37.87
Os05t0506600-00	Rice	cytosol	34.83	37.84
TraesCS1A01G126100.1	Wheat	extracellular	34.83	37.84
Os06t0157000-01	Rice	extracellular	32.09	37.83
EES06644	Sorghum	extracellular, plasma membrane, vacuole	34.33	37.81
KXG31572	Sorghum	extracellular	36.57	37.79
TraesCS1D01G390000.1	Wheat	plastid	24.63	37.79
Os01t0223000-01	Rice	plastid	36.07	37.76
HORVU7Hr1G024240.1	Barley	mitochondrion	36.82	37.76
OQU78788	Sorghum	cytosol	33.33	37.75
Zm00001d008927_P001	Maize	endoplasmic reticulum, extracellular, vacuole	24.13	37.74
TraesCS1B01G145300.1	Wheat	extracellular	34.83	37.74
HORVU5Hr1G061860.1	Barley	extracellular	35.07	37.7
GSMUA_Achr7P14040_001	Banana	cytosol	39.3	37.62
EES17880	Sorghum	golgi	35.82	37.6
HORVU2Hr1G122330.1	Barley	endoplasmic reticulum, extracellular	37.31	37.59
TraesCS7A01G115300.1	Wheat	plasma membrane	34.58	37.57
TraesCS3D01G146000.1	Wheat	cytosol, peroxisome, plasma membrane	37.31	37.5
TraesCS5A01G214900.1	Wheat	extracellular	34.83	37.43
HORVU3Hr1G028050.2	Barley	golgi, mitochondrion, plasma membrane, plastid	36.57	37.4
HORVU3Hr1G116910.1	Barley	extracellular, golgi, plasma membrane	35.07	37.4
HORVU3Hr1G116930.1	Barley	extracellular, plasma membrane	34.58	37.37
TraesCS3B01G163800.1	Wheat	extracellular	33.83	37.36
HORVU1Hr1G002220.2	Barley	endoplasmic reticulum, golgi	36.57	37.31
TraesCS3B01G163500.1	Wheat	plasma membrane	29.6	37.3
TraesCSU01G158200.1	Wheat	extracellular, golgi, vacuole	34.33	37.3
TraesCSU01G168000.1	Wheat	extracellular, golgi, vacuole	34.33	37.3
TraesCS2D01G479800.1	Wheat	extracellular, vacuole	27.36	37.29
TraesCS3B01G608400.1	Wheat	golgi, plasma membrane	34.83	37.23
HORVU1Hr1G002210.3	Barley	extracellular, golgi	37.81	37.16
HORVU3Hr1G028080.1	Barley	endoplasmic reticulum, plasma membrane	35.82	37.11
TraesCS3A01G535400.1	Wheat	extracellular	34.58	37.07
TraesCS5B01G208900.1	Wheat	extracellular	33.08	37.05
HORVU7Hr1G024220.2	Barley	endoplasmic reticulum, mitochondrion, plastid	35.57	37.05
EES02226	Sorghum	extracellular, vacuole	38.06	36.96
TraesCS5D01G294700.1	Wheat	extracellular, vacuole	29.1	36.91
TraesCSU01G015000.1	Wheat	cytosol	12.94	36.88
TraesCS2B01G505300.1	Wheat	plasma membrane, vacuole	34.83	36.84
Zm00001d046136_P001	Maize	extracellular, plasma membrane	38.56	36.82
TraesCS3D01G541000.1	Wheat	extracellular	34.58	36.77
TraesCS2A01G543700.1	Wheat	vacuole	36.57	36.75
HORVU3Hr1G027990.1	Barley	mitochondrion, plastid	37.81	36.72
EES02264	Sorghum	extracellular	33.08	36.64
TraesCS1B01G407300.1	Wheat	endoplasmic reticulum, plasma membrane, vacuole	34.08	36.63
TraesCS1B01G407500.1	Wheat	vacuole	34.08	36.63
EER87874	Sorghum	extracellular, plasma membrane	35.32	36.6
HORVU3Hr1G030080.3	Barley	golgi	36.32	36.41
Os01t0215000-01	Rice	extracellular	34.83	36.36
Zm00001d036241_P001	Maize	extracellular	33.58	36.29
TraesCS3D01G258000.3	Wheat	extracellular	36.57	36.12
TraesCS2B01G573900.1	Wheat	endoplasmic reticulum, vacuole	30.35	36.09
Zm00001d016031_P002	Maize	endoplasmic reticulum, extracellular, vacuole	34.33	36.03
Zm00001d037828_P001	Maize	plasma membrane	27.36	35.95
TraesCS1D01G389900.1	Wheat	endoplasmic reticulum, extracellular, golgi	33.58	35.9
Os06t0156700-01	Rice	plastid	36.07	35.89
HORVU6Hr1G042550.2	Barley	plasma membrane, vacuole	36.32	35.87
HORVU7Hr1G024250.2	Barley	plasma membrane	34.33	35.29
HORVU7Hr1G063430.1	Barley	mitochondrion	30.35	35.26
CDY41414	Canola	vacuole	28.61	35.06
TraesCS5B01G532500.1	Wheat	plasma membrane	33.33	34.99
TraesCS7A01G113900.1	Wheat	golgi	33.83	34.87
HORVU1Hr1G029460.3	Barley	endoplasmic reticulum, plasma membrane	33.58	34.79
CDX70269	Canola	extracellular	28.36	34.76
Os05t0210400-00	Rice	endoplasmic reticulum, plasma membrane	37.31	34.56
TraesCS7A01G348600.1	Wheat	golgi, nucleus	38.56	34.52
TraesCS7B01G011600.1	Wheat	plasma membrane, vacuole	33.58	34.35
TraesCS7D01G109200.1	Wheat	plasma membrane	33.33	34.18
TraesCS6D01G008900.2	Wheat	plastid	36.32	34.11
Zm00001d038645_P001	Maize	extracellular, vacuole	36.32	34.03
KXG31571	Sorghum	extracellular, plasma membrane, plastid	33.58	34.01
TraesCS5D01G368100.1	Wheat	plasma membrane	26.87	33.64
Zm00001d045302_P001	Maize	extracellular	35.07	33.41
HORVU7Hr1G079810.1	Barley	plastid	38.31	33.33
TraesCS1A01G382200.1	Wheat	plastid	33.83	33.33
TraesCS5A01G358900.1	Wheat	plasma membrane	32.59	33.25
GSMUA_Achr3P03520_001	Banana	endoplasmic reticulum, plasma membrane	35.82	32.88
Os01t0216900-00	Rice	peroxisome, plastid, vacuole	33.83	32.85
EES05213	Sorghum	plasma membrane	31.34	32.39
HORVU1Hr1G084390.7	Barley	plastid	33.83	32.0
TraesCS3B01G290800.1	Wheat	plastid	36.82	31.62
HORVU6Hr1G013700.3	Barley	endoplasmic reticulum, mitochondrion, plasma membrane	32.34	31.1
Zm00001d039368_P003	Maize	cytosol, endoplasmic reticulum, nucleus, plastid	34.33	30.87
HORVU2Hr1G003770.7	Barley	plastid	29.85	30.69
HORVU7Hr1G079720.2	Barley	plastid	37.56	29.84
Os01t0216000-01	Rice	plasma membrane	27.86	28.21
VIT_10s0003g01010.t01	Wine grape	cytosol	50.0	26.84
Zm00001d008925_P002	Maize	extracellular, golgi, plasma membrane	28.61	19.83
Os01t0216400-00	Rice	extracellular, vacuole	13.18	13.87
Os05t0506800-01	Rice	extracellular	1.24	1.33

Protein Annotations

EnsemblPlants:CDX77066	EnsemblPlantsGene:GSBRNA2T00127642001	Gene3D:3.40.50.1110	GO:A0A078CIS8	GO:GO:0003674	GO:GO:0003824
GO:GO:0016787	GO:GO:0016788	InterPro:GDSL	InterPro:IPR036514	InterPro:SGNH_hydro_sf	InterPro:SGNH_plant_lipase_like
PANTHER:PTHR22835	PANTHER:PTHR22835:SF293	PFAM:PF00657	ProteinID:CDX77066	ProteinID:CDX77066.1	SEG:seg
SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI0004EF05DD	MapMan:35.1	:	:

Description

BnaC04g38610D

Coordinates

chrLK031809:+:866838..868808

Molecular Weight (calculated)

44678.5 Da

IEP (calculated)

9.319

GRAVY (calculated)

-0.118

Length

402 amino acids

Sequence
(BLAST)

001: MKINILVIVA FSFLIAARSL PVKQTLNYES IFNFGDSLSD TGNFLISSDV NSPSIGRPPY GQTFFNRSTG RCSDGRLIID FIAEANGLPY VIPYLQSVRT
101: NFSVDFNKGA NFAVAGATAN DFNFLKERGL SVTLLTNKTL DVQLDWFMKL KPSLCKTKAV RFWKIPDKKP EKKTEFILFL ADCDQYFKKS LFFVGEIGGN
201: DYNYPLLAFR SYKHAIDLVP SVINKIINVT SVLIEEGAVT LVVPGNLPIG CSAVLLERFS DDNRWLYDTR NHCLKPLNNI AKLHNAKLQE GLATLRQKYT
301: HAKIIYADYY GSAMQFFNSP SKYGFTGSVL KACCGGEDGR YNAKPNVKCG GQGSTTCENP STYANWDGIH LTEAAYRHIA TGLISGRFTK PSLSNSSSFS
401: KF

Best Arabidopsis Sequence Match ( AT5G45910.1 )
(BLAST)

001: MRINMLFIVA FSFLVSVRSL PMRPTLKYES IFNFGDSLSD TGNFLLSGDV DSPNIGRLPY GQTFFNRSTG RCSDGRLIID FIAEASGLPY IPPYLQSLRT
101: NDSVDFKRGA NFAVAGATAN EFSFFKNRGL SVTLLTNKTL DIQLDWFKKL KPSLCKTKPE CEQYFRKSLF LVGEIGGNDY NYPLLAFRSF KHAMDLVPFV
201: INKIMDVTSA LIEEGAMTLI VPGNLPIGCS AALLERFNDN SGWLYDSRNQ CYMPLNNLAK LHNDKLKKGL AALRKKYPYA KIIYADYYSS AMQFFNSPSK
301: YGFTGSVLKA CCGGGDGRYN VQPNVRCGEK GSTTCEDPST YANWDGIHLT EAAYRHIATG LISGRFTMPT YN

Arabidopsis Description

GDSL esterase/lipase At5g45910 [Source:UniProtKB/Swiss-Prot;Acc:Q9FJ45]
SUBAcon: [extracellular]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.