CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

plastid 3
endoplasmic reticulum 2
plasma membrane 4
extracellular 1
vacuole 1
golgi 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	plastid, secretory
BaCelLo:	plastid
iPSORT:	plastid
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	plasma membrane
Predotar:	plastid
PProwler:	secretory
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH31638
KRH69070

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G23170.1	KRG89812	AT2G41090.1	17360592
AT4G23170.1	KRG94975	AT2G41090.1	17360592
AT4G23170.1	KRH18664	AT2G41090.1	17360592
AT4G23170.1	KRG89812	AT2G41100.1	17360592
AT4G23170.1	KRG94975	AT2G41100.1	17360592
AT4G23170.1	KRH18664	AT2G41100.1	17360592
AT4G23170.1	KRH31638	AT4G14640.1	17360592
AT4G23170.1	KRH69070	AT4G14640.1	17360592
AT4G23170.1	KRG89812	AT5G21274.1	17360592
AT4G23170.1	KRG94975	AT5G21274.1	17360592
AT4G23170.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG91211	Soybean	plasma membrane	91.94	91.67
KRH26837	Soybean	cytosol	22.99	44.25
KRH22514	Soybean	endoplasmic reticulum, extracellular	28.36	38.93
KRH22515	Soybean	endoplasmic reticulum, extracellular	28.36	38.78
KRH26839	Soybean	cytosol	23.28	38.61
CDY41770	Canola	endoplasmic reticulum, vacuole	28.96	37.31
HORVU3Hr1G109450.1	Barley	endoplasmic reticulum	13.43	35.43
Bra040999.1-P	Field mustard	extracellular	26.27	34.92
CDY01121	Canola	extracellular	26.27	34.92
CDY47325	Canola	endoplasmic reticulum, extracellular, vacuole	25.97	34.39
KRH35616	Soybean	extracellular	26.57	34.1
AT4G23170.1	Thale cress	extracellular	26.87	33.96
KRH35615	Soybean	endoplasmic reticulum, extracellular	23.28	33.77
CDY12405	Canola	plasma membrane	29.25	33.45
KRH22513	Soybean	cytosol	16.42	33.33
KRG91170	Soybean	plasma membrane	25.97	30.96
KRG99540	Soybean	extracellular	21.49	28.02
KRH35619	Soybean	extracellular, plasma membrane, vacuole	25.97	27.27
CDY10852	Canola	plasma membrane	30.75	27.11
Os07t0537300-00	Rice	extracellular	23.28	27.08
KRH35620	Soybean	plasma membrane	26.27	26.83
TraesCS3D01G025300.1	Wheat	extracellular, plasma membrane	22.09	26.52
Os07t0537200-01	Rice	plasma membrane	22.99	26.46
TraesCS3B01G024400.1	Wheat	extracellular	22.09	26.33
KRH21653	Soybean	extracellular	21.19	25.36
TraesCS2B01G242200.1	Wheat	endoplasmic reticulum, plasma membrane	22.99	25.25
VIT_02s0087g01010.t01	Wine grape	plasma membrane	33.43	25.11
Zm00001d021693_P001	Maize	endoplasmic reticulum, plasma membrane	21.49	25.09
CDY41240	Canola	extracellular, plasma membrane	28.06	24.74
TraesCS2D01G222200.1	Wheat	endoplasmic reticulum, plasma membrane	21.49	24.16
KRH13264	Soybean	mitochondrion	17.01	24.15
HORVU3Hr1G002810.2	Barley	extracellular	21.49	23.0
TraesCSU01G233400.1	Wheat	extracellular	19.1	22.94
KRG91167	Soybean	endoplasmic reticulum, extracellular, plasma membrane	14.63	22.17
HORVU2Hr1G125180.2	Barley	extracellular	18.51	21.75
CDY15079	Canola	cytosol, plasma membrane, plastid	21.79	21.53
KRH35613	Soybean	plasma membrane	24.18	21.49
TraesCS2A01G216800.1	Wheat	endoplasmic reticulum, vacuole	20.9	21.28
Bra019321.1-P	Field mustard	mitochondrion	17.61	20.56
TraesCS2B01G242400.1	Wheat	plastid	22.09	19.68
TraesCS1D01G431100.1	Wheat	extracellular	17.91	19.29
TraesCS3D01G501300.3	Wheat	plasma membrane	22.99	18.97
Os07t0540600-00	Rice	endoplasmic reticulum, plasma membrane	20.9	18.72
CDY10860	Canola	endoplasmic reticulum, plasma membrane	15.22	18.02
HORVU3Hr1G109380.8	Barley	mitochondrion	13.13	17.89
CDY39159	Canola	plasma membrane	31.64	17.61
Bra029565.1-P	Field mustard	plasma membrane	32.24	17.56
TraesCS2D01G222500.1	Wheat	vacuole	21.49	17.52
Zm00001d040823_P001	Maize	plasma membrane	22.39	15.18
Os10t0329900-00	Rice	endoplasmic reticulum, plasma membrane	18.21	15.1
TraesCS3A01G494000.1	Wheat	plasma membrane	22.69	14.84
Zm00001d021683_P001	Maize	endoplasmic reticulum, extracellular, plasma membrane	20.6	13.56
TraesCS3D01G501200.1	Wheat	plasma membrane	23.88	11.49
Zm00001d030212_P001	Maize	plasma membrane	16.72	11.05

Protein Annotations

EMBL:ACUP02012668	EnsemblPlants:KRG91214	EnsemblPlantsGene:GLYMA_20G140300	EntrezGene:102666772	Gene3D:3.30.430.20	GO:GO:0005575
GO:GO:0016020	GO:GO:0016021	InterPro:GNK2	InterPro:GNK2_sf	InterPro:IPR002902	InterPro:IPR038408
PANTHER:PTHR32099	PANTHER:PTHR32099:SF13	PFAM:PF01657	PFscan:PS51473	ProteinID:KRG91214	ProteinID:KRG91214.1
SEG:seg	TMHMM:TMhelix	UniParc:UPI00023DBA5F	UniProt:K7N3E1	MapMan:35.1	:

Description

hypothetical protein

Coordinates

chr20:+:37909244..37911500

Molecular Weight (calculated)

37024.8 Da

IEP (calculated)

8.187

GRAVY (calculated)

0.120

Length

335 amino acids

Sequence
(BLAST)

001: MYDPLQIFLQ TKIPKMPPCK YFAIPSILFF LLSFLIFPTK AAPIYSSHAC TDSSKYQPNS TFQTNLDLLL SSLSSNATQG VHFYKTTVGS ETPNAVKGLF
101: LCRGDTLAAV CHDCVNAAAK DLTRRCPVEK EAIIWYDVCM VRYSNQYYLN NIVPAVDMSD SKSVAGADLD RFNEVLAGLL NALATKAANS EDEKFETGEV
201: NLTSSVTLYG LVQCTPELSL FDCNMCFRSA IASVPNCCDG KQGARVLLPG CNIRYQVYPF YNSTKILATP VVKSRPSGRN RVEVNLTFVI PIVAAMVLFT
301: FGICSVMKKQ AKSEIEIWRK TVLTASFWGG KTELF

Best Arabidopsis Sequence Match ( AT4G05200.1 )
(BLAST)

001: MSSCFKSSVS LFSVFLFMIL KTVTSDPTYL YHICPNTTTY SRNSSYLTNL RTVLSSLSSP NAAYASLFDN AAAGEENDSN RVYGVFLCRG DVSAEICRDC
101: VAFAANETLQ RCPREKVAVI WYDECMVRYS NQSIVGQMRI RPGVFLTNKQ NITENQVSRF NESLPALLID VAVKAALSSR KFATEKANFT VFQTIYSLVQ
201: CTPDLTNQDC ESCLRQVINY LPRCCDRSVG GRVIAPSCSF RYELYPFYNE TIAAAPMAPP PSSTVTAPPL NIPSEKGKGK NLTVIVTAIA VPVSVCVLLL
301: GAMCWLLARR RNNKLSAETE DLDEDGITST ETLQFQFSAI EAATNKFSES NKLGHGGFGE VYKGQLITGE TVAIKRLSQG STQGAEEFKN EVDVVAKLQH
401: RNLAKLLGYC LDGEEKILVY EFVPNKSLDY FLFDNEKRRV LDWQRRYKII EGIARGILYL HRDSRLTIIH RDLKASNILL DADMHPKISD FGMARIFGVD
501: QTQANTKRIV GTYGYMSPEY AIHGKYSVKS DVYSFGVLVL ELITGKKNSS FYEEDGLGDL VTYVWKLWVE NSPLELVDEA MRGNFQTNEV IRCIHIALLC
601: VQEDSSERPS MDDILVMMNS FTVTLPIPKR SGFLLRTMKD SRDPRSGGSA SDHSATSKSL PLSVDDSSIT IVYPR

Arabidopsis Description

CRK25cysteine-rich RLK (RECEPTOR-like protein kinase) 25 [Source:TAIR;Acc:AT4G05200]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.