Factsheet for Os02t0519900-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, plastid, nucleus

Predictor Summary:

cytosol 6
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 18260611
plasma membrane: 19502382
nucleus: 19621931
extracellular: 21255809
plastid: 21433289
nucleus: 26035336
plastid: 27992503

msms PMID: 19502382 doi

M Fujiwara, S Hamada, M Hiratsuka, Y Fukao, T Kawasaki, K Shimamoto

Laboratory of Plant Protein Analysis, Plant Education Unit, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.

msms PMID: 26035336 doi

M Li, X Yin, K Sakata, P Yang, S Komatsu

§Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan., †Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China., ‡National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

msms PMID: 21433289 doi

S Reiland, J Grossmann, K Baerenfaller, P Gehrig, A Nunes-Nesi, AR Fernie, W Gruissem, S Baginsky

Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland.

msms PMID: 21255809 doi

T Shinano, S Komatsu, T Yoshimura, S Tokutake, FJ Kong, T Watanabe, J Wasaki, M Osaki

National Agriculture and Food Research Organization, National Agricultural Research Center for Hokkaido Region, 1-Hitsujigaoka, Toyohira-ku, Sapporo 062-8555, Japan. shinano@affrc.go.jp

msms PMID: 19621931 doi

T Aki, S Yanagisawa

Department of Applied Biological Chemistry, The University of Tokyo, Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os04t0118400-01	Rice	extracellular	98.81	98.81
Solyc12g070080.1.1	Tomato	cytosol, plastid	2.85	16.11
Os12t0169000-00	Rice	plasma membrane	11.03	15.68
Os04t0102700-01	Rice	plasma membrane	11.27	15.62
Os11t0170000-01	Rice	peroxisome	11.27	15.57
Os04t0118100-01	Rice	cytosol	7.83	15.17
Os04t0117900-01	Rice	plasma membrane, plastid	7.35	14.29
Os04t0643200-00	Rice	plastid	9.02	14.23
Os02t0754500-01	Rice	cytosol, mitochondrion, plastid	10.2	14.03
Os03t0707900-01	Rice	plasma membrane	9.73	13.99
CDX69906	Canola	cytosol, plastid	9.61	13.43
Os11t0536800-01	Rice	golgi, plastid	8.9	11.21
Solyc12g070090.1.1	Tomato	cytosol	2.85	11.11
Os04t0102600-01	Rice	mitochondrion	0.36	1.46
Os11t0170200-01	Rice	nucleus	0.12	0.11
CDX69905	Canola	mitochondrion	0.0	0.0
Bra022265.1-P	Field mustard	cytosol, mitochondrion	0.0	0.0

Protein Annotations

MapMan:17.5.2.1	Gene3D:2.40.30.10	Gene3D:3.30.230.10	Gene3D:3.30.70.240	Gene3D:3.30.70.870	Gene3D:3.40.50.300
Gene3D:3.90.1430.10	EntrezGene:4329493	ProteinID:BAD26337.1	ProteinID:BAF08886.1	ProteinID:BAS78939.1	ProteinID:BAS78941.1
ProteinID:EAZ23241.1	EMBL:EF122482	InterPro:EFG_III/V	InterPro:EFG_V-like	InterPro:EFTu-like_2	GO:GO:0000166
GO:GO:0003674	GO:GO:0003676	GO:GO:0003723	GO:GO:0003746	GO:GO:0003824	GO:GO:0003924
GO:GO:0005488	GO:GO:0005525	GO:GO:0006412	GO:GO:0006414	GO:GO:0008135	GO:GO:0008150
GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016787	GO:GO:0019538	InterPro:G_TR_CS
InterPro:IPR000795	InterPro:IPR014721	EMBL:KM051839	EnsemblPlantsGene:Os02g0519900	EnsemblPlants:Os02t0519900-01	InterPro:P-loop_NTPase
PFAM:PF00009	PFAM:PF00679	PFAM:PF03144	PFAM:PF03764	PFAM:PF14492	PRINTS:PR00315
ScanProsite:PS00301	PFscan:PS51722	PANTHER:PTHR42908	PANTHER:PTHR42908:SF11	UniProt:Q6H4L2	InterPro:Ribosomal_S5_D2-typ_fold
InterPro:Ribosomal_S5_D2-typ_fold_subgr	SMART:SM00838	SMART:SM00889	SUPFAM:SSF50447	SUPFAM:SSF52540	SUPFAM:SSF54211
SUPFAM:SSF54980	InterPro:Small_GTP-bd_dom	InterPro:TF_GTP-bd_dom	TIGRFAMs:TIGR00231	InterPro:Transl_B-barrel_sf	InterPro:Transl_elong_EFG/EF2_IV
UniParc:UPI00003B46A1	RefSeq:XP_015625235.1	:	:	:	:

Description

Similar to Elongation factor EF-2 (Fragment). (Os02t0519900-01);Similar to H0613H07.5 protein. (Os02t0519900-02);Similar to H0613H07.5 protein. (Os02t0519900-03);Similar to H0613H07.5 protein. (Os02t0519900-04);Similar to H0613H07.5 protein. (Os02t0519900-05)

Coordinates

chr2:+:18916988..18921785

Molecular Weight (calculated)

94026.8 Da

IEP (calculated)

6.031

GRAVY (calculated)

-0.208

Length

843 amino acids

Sequence
(BLAST)

001: MVKFTVEELR RIMDKKNNIR NMSVIAHVDH GKSTLTDSLV AAAGIIAQEV AGDVRMTDTR ADEAERGITI KSTGISLFYE MSDESLKLYK GERDGNEYLI
101: NLIDSPGHVD FSSEVTAALR ITDGALVVVD CIEGVCVQTE TVLRQALGER IRPVLTVNKM DRCFLELQVE GEEAYQTFSR VIENANVIMA TYEDTLLGDV
201: QVYPEKGTVA FSAGLHGWAF TLSSFAKMYA SKFGVDEFKM MERLWGENFF DPATKKWTNK NTGSATCKRG FVQFCYEPIK QIINTCMNDQ KDKLWPMLQK
301: LGVVMKADEK ELMGKALMKR VMQTWLPASN ALLEMMIYHL PSPSKAQRYR VENLYEGPLD DVYATAIRNC DPEGPLMLYV SKMIPASDKG RFFAFGRVFS
401: GRVATGMKVR IMGPNYVPGQ KKDLYVKSVQ RTVIWMGKKQ ESVEDVPCGN TVAMVGLDQF ITKNATLTNE KESDACPIRA MKFSVSPVVR VAVQCKVASD
501: LPKLVEGLKR LAKSDPMVLC TIEESGEHII AGAGELHLEI CLKDLQEDFM GGAEIIVSPP VVSFRETVLE KSCRTVMSKS PNKHNRLYME ARPLEEGLAE
601: AIDDGRIGPR DDPKVRSKIL SEEFGWDKDL AKKIWCFGPE TTGPNMVVDM CKGVQYLNEI KDSVVAGFQW ASKEGALAEE NMRGICFEVC DVVLHADAIH
701: RGGGQVIPTA RRVIYASQLT AKPRLLEPVY LVEIQAPENA LGGIYGVLNQ KRGHVFEEMQ RPGTPLYNIK AYLPVIESFG FSSQLRAATS GQAFPQCVFD
801: HWDMMTSDPL EAGSQASTLV QDIRKRKGLK EQMTPLSDFE DKL

Best Arabidopsis Sequence Match ( AT1G56070.1 )
(BLAST)

001: MVKFTADELR RIMDYKHNIR NMSVIAHVDH GKSTLTDSLV AAAGIIAQEV AGDVRMTDTR ADEAERGITI KSTGISLYYE MTDESLKSFT GARDGNEYLI
101: NLIDSPGHVD FSSEVTAALR ITDGALVVVD CIEGVCVQTE TVLRQALGER IRPVLTVNKM DRCFLELQVD GEEAYQTFSR VIENANVIMA TYEDPLLGDV
201: QVYPEKGTVA FSAGLHGWAF TLTNFAKMYA SKFGVVESKM MERLWGENFF DPATRKWSGK NTGSPTCKRG FVQFCYEPIK QIIATCMNDQ KDKLWPMLAK
301: LGVSMKNDEK ELMGKPLMKR VMQTWLPAST ALLEMMIFHL PSPHTAQRYR VENLYEGPLD DQYANAIRNC DPNGPLMLYV SKMIPASDKG RFFAFGRVFA
401: GKVSTGMKVR IMGPNYIPGE KKDLYTKSVQ RTVIWMGKRQ ETVEDVPCGN TVAMVGLDQF ITKNATLTNE KEVDAHPIRA MKFSVSPVVR VAVQCKVASD
501: LPKLVEGLKR LAKSDPMVVC TMEESGEHIV AGAGELHLEI CLKDLQDDFM GGAEIIKSDP VVSFRETVCD RSTRTVMSKS PNKHNRLYME ARPMEEGLAE
601: AIDDGRIGPR DDPKIRSKIL AEEFGWDKDL AKKIWAFGPE TTGPNMVVDM CKGVQYLNEI KDSVVAGFQW ASKEGPLAEE NMRGICFEVC DVVLHSDAIH
701: RGGGQVIPTA RRVIYASQIT AKPRLLEPVY MVEIQAPEGA LGGIYSVLNQ KRGHVFEEMQ RPGTPLYNIK AYLPVVESFG FSSQLRAATS GQAFPQCVFD
801: HWEMMSSDPL EPGTQASVLV ADIRKRKGLK EAMTPLSEFE DKL

Arabidopsis Description

LOS1Elongation factor 2 [Source:UniProtKB/Swiss-Prot;Acc:Q9ASR1]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.