Factsheet for Os03t0688300-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 2
cytosol 3
mitochondrion 1
plastid 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0267900-01
Os01t0279300-01
Os01t0810300-01
Os03t0319300-01
Os03t0743500-02
Os05t0491000-01
Os05t0491100-01
Os07t0687200-02
Os12t0603800-00

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G74740.1	Os01t0267900-01	AT2G41090.1	17360592
AT1G74740.1	Os01t0279300-01	AT2G41090.1	17360592
AT1G74740.1	Os01t0810300-01	AT2G41090.1	17360592
AT1G74740.1	Os03t0319300-01	AT2G41090.1	17360592
AT1G74740.1	Os05t0491000-01	AT2G41090.1	17360592
AT1G74740.1	Os05t0491100-01	AT2G41090.1	17360592
AT1G74740.1	Os07t0687200-02	AT2G41090.1	17360592
AT1G74740.1	Os12t0603800-00	AT2G41090.1	17360592
AT1G74740.1	Os01t0267900-01	AT2G41100.1	17360592
AT1G74740.1	Os01t0279300-01	AT2G41100.1	17360592
AT1G74740.1	Os01t0810300-01	AT2G41100.1	17360592
AT1G74740.1	Os03t0319300-01	AT2G41100.1	17360592
AT1G74740.1	Os05t0491000-01	AT2G41100.1	17360592
AT1G74740.1	Os05t0491100-01	AT2G41100.1	17360592
AT1G74740.1	Os07t0687200-02	AT2G41100.1	17360592
AT1G74740.1	Os12t0603800-00	AT2G41100.1	17360592
AT1G74740.1	Os03t0743500-02	AT4G14640.1	17360592
AT1G74740.1	Os01t0267900-01	AT5G21274.1	17360592
AT1G74740.1	Os01t0279300-01	AT5G21274.1	17360592
AT1G74740.1	Os01t0810300-01	AT5G21274.1	17360592
AT1G74740.1	Os03t0319300-01	AT5G21274.1	17360592
AT1G74740.1	Os05t0491000-01	AT5G21274.1	17360592
AT1G74740.1	Os05t0491100-01	AT5G21274.1	17360592
AT1G74740.1	Os07t0687200-02	AT5G21274.1	17360592
AT1G74740.1	Os12t0603800-00	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS4D01G027600.1	Wheat	plastid	81.18	81.75
TraesCS4A01G283400.1	Wheat	plastid	80.84	81.55
Zm00001d013527_P002	Maize	cytosol, plastid	82.58	81.03
EER93646	Sorghum	plastid	81.88	80.2
GSMUA_Achr2P17730_001	Banana	cytosol	56.1	79.9
HORVU4Hr1G004540.20	Barley	plastid	81.36	77.57
TraesCS4B01G030100.1	Wheat	plastid	80.66	77.17
VIT_17s0000g05520.t01	Wine grape	cytosol	56.79	75.64
PGSC0003DMT400034291	Potato	cytosol	65.85	70.26
Solyc03g113390.2.1	Tomato	cytosol	65.85	70.26
KRH54494	Soybean	cytosol	66.55	69.33
KRH56973	Soybean	cytosol	64.98	69.2
KRH63420	Soybean	cytosol	66.9	69.06
KRH03406	Soybean	cytosol	64.98	68.95
CDY45391	Canola	cytosol	64.81	68.13
Bra015896.1-P	Field mustard	cytosol	64.63	67.95
CDX73108	Canola	cytosol	64.63	67.82
CDX81903	Canola	cytosol, plastid	64.11	67.77
Bra031055.1-P	Field mustard	cytosol, plastid	62.37	67.42
AT1G18890.1	Thale cress	cytosol, plastid	63.76	67.16
AT1G74740.1	Thale cress	cytosol	63.24	67.1
CDY21691	Canola	cytosol, plastid	63.94	67.09
Bra025696.1-P	Field mustard	cytosol, plastid	59.58	64.77
Os05t0467000-01	Rice	cytosol, plasma membrane	60.45	63.44
Os01t0832300-00	Rice	cytosol, plasma membrane	60.28	62.79
Os07t0568600-01	Rice	cytosol, plasma membrane	58.71	61.27
CDY26714	Canola	cytosol	64.98	61.25
Os03t0808600-01	Rice	plasma membrane	57.32	61.15
CDY19259	Canola	cytosol, plastid	64.98	60.85
Os12t0230200-02	Rice	plasma membrane	53.83	54.88
Os08t0540400-01	Rice	plasma membrane	52.09	52.92
Os02t0685900-00	Rice	plasma membrane	48.26	50.46
Os12t0133500-00	Rice	plasma membrane	47.21	50.09
Os09t0514200-01	Rice	mitochondrion	50.17	49.91
Os03t0128700-01	Rice	mitochondrion	42.86	45.39
Os07t0619800-02	Rice	cytosol, plasma membrane	32.58	41.28
Os07t0409900-01	Rice	plasma membrane	36.06	40.43
Os12t0487250-01	Rice	plastid	30.49	38.89
Os02t0126400-01	Rice	cytosol	34.67	38.12
Os03t0366200-01	Rice	cytosol, plasma membrane	17.94	35.64
Os07t0641200-01	Rice	plasma membrane	14.29	31.3
Os06t0714200-01	Rice	plasma membrane	33.8	31.14
Os10t0510700-00	Rice	plasma membrane	32.93	25.89
Os07t0641000-01	Rice	mitochondrion	1.92	3.77

Protein Annotations

Gene3D:1.10.238.10	Gene3D:1.10.510.10	MapMan:18.4.5.4	Gene3D:3.30.200.20	EntrezGene:4333767	ProteinID:AAT81734.1
ProteinID:ABF98270.1	EMBL:AK105102	ProteinID:BAF12858.1	ProteinID:BAS85825.1	InterPro:EF-hand-dom_pair	InterPro:EF_Hand_1_Ca_BS
InterPro:EF_hand_dom	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674
GO:GO:0004683	GO:GO:0005488	GO:GO:0005509	GO:GO:0005515	GO:GO:0005516	GO:GO:0005524
GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005737	GO:GO:0005886
GO:GO:0006464	GO:GO:0006468	GO:GO:0006950	GO:GO:0006970	GO:GO:0007154	GO:GO:0007165
GO:GO:0007275	GO:GO:0008150	GO:GO:0008152	GO:GO:0009628	GO:GO:0009719	GO:GO:0009738
GO:GO:0009931	GO:GO:0009987	GO:GO:0010152	GO:GO:0016020	GO:GO:0016301	GO:GO:0016310
GO:GO:0016740	GO:GO:0018105	GO:GO:0019538	GO:GO:0035556	GO:GO:0046777	GO:GO:0046872
GO:GO:1902584	InterPro:IPR000719	InterPro:IPR002048	InterPro:Kinase-like_dom_sf	EnsemblPlantsGene:Os03g0688300	EnsemblPlants:Os03t0688300-01
PFAM:PF00069	PFAM:PF13499	ScanProsite:PS00018	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PFscan:PS50222	PANTHER:PTHR24349	PANTHER:PTHR24349:SF167	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	UniProt:Q6AVI8
SMART:SM00054	SMART:SM00220	SUPFAM:SSF47473	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI0000400336
RefSeq:XP_015631324.1	SEG:seg	:	:	:	:

Description

CALCIUM-DEPENDENT PROTEIN KINASE 9Calcium-dependent protein kinase, Positive regulator of drought stress tolerance and spikelet fertility (Os03t0688300-01)

Coordinates

chr3:+:27467413..27472746

Molecular Weight (calculated)

63981.5 Da

IEP (calculated)

8.723

GRAVY (calculated)

-0.420

Length

574 amino acids

Sequence
(BLAST)

001: MGNTCCVAPA TTDEVGAPPR DHHHAAKKSP APSATTTTAT RQRHGQEPKP KPKPRARAKP NPYDWAPPRV LPARGGAAAS AVRVLEGVVP HHPRLRVTDK
101: YQLGRELGRG EFGVTHLATD RATRERLACK SIPKRRLRTA VDVADVRREV AIMASLPDHP ALVRLRAAYE DADAVHLVME LCDGGELFDR IVARGRYTER
201: AAAAAARTVA EVVRACHAHG VMHRDLKPEN FLYAGKAEDA QLKAIDFGLS VFFRPGERFR EIVGSPYYMA PEVLRRDYGP EVDIWSAGVI LYILLCGVPP
301: FWAETEQGVA RAILRGAADF DREPWPRISR AAKSLVRQML DVDPRRRPTA QQVLDHPWLH HAARAPNVPL GDVVRARLKQ FSLMNRLKKK AMRVIAEHLS
401: VEEVEVIKDM FALMDTDNNG RVTLQELKDG LTKVGSKLAE PEMELLMEAA DVDGNGYLDY GEFVAVTIHL QRLSNDNHLR TAFLFFDKDG SGYIDRAELA
501: DALADDSGHA DDAVLDHILR EVDTDKDGRI SYEEFVAMMK SGTDWRKASR QYSRERFKTL SNSLIKDGSI TMAR

Best Arabidopsis Sequence Match ( AT1G74740.1 )
(BLAST)

001: MGNCIACVKF DPDNSKPNQK KKPPRGRQRN PYDDPDGLRT HAPLRVIPMS HQSQISDKYI LGRELGRGEF GITYLCTDRE TREALACKSI SKRKLRTAVD
101: VEDVRREVTI MSTLPEHPNV VKLKATYEDN ENVHLVMELC EGGELFDRIV ARGHYTERAA ATVARTIAEV VRMCHVNGVM HRDLKPENFL FANKKENSAL
201: KAIDFGLSVL FKPGERFTEI VGSPYYMAPE VLKRNYGPEV DVWSAGVILY ILLCGVPPFW AETEQGVALA ILRGVLDFKR DPWSQISESA KSLVKQMLEP
301: DSTKRLTAQQ VLDHPWIQNA KKAPNVPLGD IVRSRLKQFS MMNRLKKKAL RVIAEHLSIQ EVEVIRNMFT LMDDDNDGKI SYLELRAGLR KVGSQLGEPE
401: IKLLMEVADV NGNGCLDYGE FVAVIIHLQK MENDEHFRQA FMFFDKDGSG YIESEELREA LTDELGEPDN SVIIDIMREV DTDKDGKINY DEFVVMMKAG
501: TDWRKASRQY SRERFKSLSL NLMKDGSMHL HDALTGQSIA V

Arabidopsis Description

CPK30Calcium-dependent protein kinase 30 [Source:UniProtKB/Swiss-Prot;Acc:Q9SSF8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.