Factsheet for Zm00001d008268_P002

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, extracellular

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 4
plasma membrane 4
golgi 4
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum, extracellular
Any Predictor:	secretory
iPSORT:	secretory
MultiLoc:	extracellular
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	endoplasmic reticulum

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EES02749	Sorghum	extracellular	82.86	84.58
TraesCS3D01G189800.1	Wheat	plastid	73.88	74.49
TraesCS3A01G185800.1	Wheat	extracellular	73.88	74.49
GSMUA_AchrUn_...	Banana	cytosol	28.98	73.96
TraesCS3B01G215400.1	Wheat	extracellular	73.06	73.66
Os01t0270100-01	Rice	plasma membrane	73.06	71.6
VIT_13s0084g00050.t01	Wine grape	cytosol	52.24	63.68
CDY42150	Canola	cytosol	51.84	62.56
CDY50343	Canola	cytosol	50.2	60.0
Bra038725.1-P	Field mustard	cytosol	49.8	59.51
HORVU3Hr1G038190.1	Barley	endoplasmic reticulum, golgi, nucleus	73.06	58.69
AT3G12490.2	Thale cress	extracellular	55.51	58.12
Bra034755.1-P	Field mustard	extracellular	51.43	56.5
CDY08429	Canola	extracellular	52.65	56.33
PGSC0003DMT400068064	Potato	cytosol	46.12	56.22
CDY08430	Canola	extracellular	52.24	54.7
KRH20612	Soybean	endoplasmic reticulum	51.84	51.84
KRH13275	Soybean	mitochondrion	51.02	51.02
Solyc03g097270.2.1	Tomato	extracellular	47.35	49.36
CDY03938	Canola	extracellular	41.63	48.57
CDY50523	Canola	cytosol	28.98	47.33
Zm00001d038558_P003	Maize	cytosol	20.41	45.45
Zm00001d012561_P001	Maize	extracellular	22.86	41.79
Zm00001d043175_P001	Maize	extracellular	22.04	40.0
Zm00001d010290_P001	Maize	extracellular	23.27	32.39
Zm00001d038173_P003	Maize	extracellular	21.22	30.06

Protein Annotations

Gene3D:3.10.450.10	Gene3D:3.10.450.650	MapMan:35.1	EntrezGene:732753	ProteinID:AQK89345.1	ProteinID:AQK89346.1
UniProt:B4FV48	EMBL:BT040986	InterPro:Cystatin	InterPro:Cystatin_dom	EMBL:EU960590	GO:GO:0003674
GO:GO:0004869	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0010466	GO:GO:0010951
GO:GO:0019538	GO:GO:0030234	GO:GO:0030414	PFAM:PD001231	PFAM:PF16845	ScanProsite:PS00287
PFscan:PS51257	PANTHER:PTHR11413	PANTHER:PTHR11413:SF56	InterPro:Prot_inh_cystat_CS	SMART:SM00043	SUPFAM:SSF54403
SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI000050993C	EnsemblPlantsGene:Zm00001d008268	EnsemblPlants:Zm00001d008268_P002	EnsemblPlants:Zm00001d008268_T002
SEG:seg	:	:	:	:	:

Description

cystatin4 cystatin4

Coordinates

chr8:+:3546648..3549333

Molecular Weight (calculated)

26697.8 Da

IEP (calculated)

6.578

GRAVY (calculated)

-0.247

Length

245 amino acids

Sequence
(BLAST)

001: MRVAATRAAA AAHPPSAFLL LLLLLGCASL AIGGAAMAGH VLGGVKENPA AANSAESDGL GRFAVDEHNR RENALLEFVR VVEAKEQVVA GTLHHLTLEA
101: VEAGRKKLYE AKVWVKPWLD FKELQEFSHK GDATAFTNAD LGAKQGGHEP GWREVPVEDP VVKDAAHHAV KSIQERSNSL FPYELLEILR AHAQVVEDFA
201: KFDILMKLKR GSKEEKIKAE VHKSLEGAFV LNQHQPAEHD ESSSQ

Best Arabidopsis Sequence Match ( AT3G12490.2 )
(BLAST)

001: MMRSRFLLFI VFFSLSLFIS SLIASDLGFC NEEMALVGGV GDVPANQNSG EVESLARFAV DEHNKKENAL LEFARVVKAK EQVVAGTLHH LTLEILEAGQ
101: KKLYEAKVWV KPWLNFKELQ EFKPASDAPA ITSSDLGCKQ GEHESGWREV PGDDPEVKHV AEQAVKTIQQ RSNSLFPYEL LEVVHAKAEV TGEAAKYNML
201: LKLKRGEKEE KFKVEVHKNH EGALHLNHAE QHHD

Arabidopsis Description

CYS6Cysteine proteinase inhibitor 6 [Source:UniProtKB/Swiss-Prot;Acc:Q8H0X6]
SUBAcon: [extracellular]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.