Factsheet for Bra034353.1-P

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 1
cytosol 3
plasma membrane 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol
Any Predictor:	cytosol, mitochondrion, nucleus, plasma membrane
BaCelLo:	nucleus
MultiLoc:	cytosol
Plant-mPloc:	plasma membrane
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY30547	Canola	cytosol	100.0	100.0
AT2G27450.2	Thale cress	cytosol	94.95	86.5
VIT_06s0080g00560.t01	Wine grape	cytosol	85.52	85.52
KRH42198	Soybean	cytosol	85.19	84.62
GSMUA_Achr6P19390_001	Banana	cytosol	79.46	84.59
PGSC0003DMT400067716	Potato	cytosol	84.85	84.0
Solyc11g068540.1.1	Tomato	endoplasmic reticulum, extracellular	84.51	83.67
KRH22668	Soybean	cytosol	84.18	83.61
KRH26651	Soybean	cytosol	84.18	83.61
Bra012018.1-P	Field mustard	cytosol	20.88	79.49
TraesCS5A01G024500.1	Wheat	cytosol	79.8	79.0
HORVU5Hr1G006730.1	Barley	cytosol, mitochondrion	79.8	78.74
EES05187	Sorghum	cytosol	78.79	78.52
TraesCS5B01G022300.1	Wheat	cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole	79.46	78.41
TraesCS5D01G031200.1	Wheat	golgi	79.46	78.41
Os02t0533900-01	Rice	plasma membrane	79.12	78.07
Zm00001d016704_P001	Maize	extracellular	77.44	76.67
Bra006138.1-P	Field mustard	plastid	25.59	20.88
Bra024290.1-P	Field mustard	cytosol	28.62	20.83
Bra037776.1-P	Field mustard	cytosol	28.28	20.59
Bra037878.1-P	Field mustard	plastid	20.54	18.71
Bra008919.1-P	Field mustard	plastid	25.59	17.67

Protein Annotations

KEGG:00330+3.5.1.53	Gene3D:3.60.110.10	MapMan:8.1.1.3	EnsemblPlantsGene:Bra034353	EnsemblPlants:Bra034353.1	EnsemblPlants:Bra034353.1-P
InterPro:C-N_Hydrolase	InterPro:C-N_Hydrolase_sf	GO:GO:0003674	GO:GO:0003824	GO:GO:0006596	GO:GO:0006807
GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009987	GO:GO:0016787	GO:GO:0016810
GO:GO:0050126	InterPro:IPR003010	InterPro:IPR036526	UniProt:M4EZW0	InterPro:N-carbamoylputrescine_amidase	PFAM:PF00795
PFscan:PS50263	PANTHER:PTHR43674	PANTHER:PTHR43674:SF7	SUPFAM:SSF56317	TIGRFAMs:TIGR03381	UniParc:UPI0002543A70

Description

AT2G27450 (E=5e-166) NLP1, ATNLP1, CPA | NLP1 (NITRILASE-LIKE PROTEIN 1); N-carbamoylputrescine amidase/ hydrolase, acting on carbon-nitrogen (but not peptide) bonds

Coordinates

chrA04:-:12075500..12077218

Molecular Weight (calculated)

33294.4 Da

IEP (calculated)

5.928

GRAVY (calculated)

-0.338

Length

297 amino acids

Sequence
(BLAST)

001: MEGRRREVVV SSLQFACSDD ISSNVASAER LVREAHAKGA NIVLIQELFE GYYFCQAQRE DYFQRAKPYK DHPTIARMQT LAKELGVVIP VSFFEEANTA
101: HYNSIAIIDA DGTDLGIYRK SHIPDGPGYQ EKFYFNPGDT GFKVFQTKFA KIGVAICWDQ WFPEAARAMV LQGAEVLFYP TAIGSEPQDQ GLDSRDHWRR
201: VMQGHAGANV VPLVASNRIG KETIETEHGP SQITFYGNSF IAGPTGEIVA EADDKTEAVL VAKFDLEKIK SKRQSWGVFR DRRPDLYKVL LTMDGNL

Best Arabidopsis Sequence Match ( AT2G27450.1 )
(BLAST)

001: METEGRRREV VVSSLQFACS DDISTNVAAA ERLVREAHAK GANIILIQEL FEGYYFCQAQ REDFFKRAKP YKNHPTIARM QKLAKELGVV IPVSFFEEAN
101: TAHYNSIAII DADGTDLGIY RKSHIPDGPG YQEKFYFNPG DTGFKVFQTK FAKIGVAICW DQWFPEAARA MVLQGAEILF YPTAIGSEPQ DQGLDSRDHW
201: RRVMQGHAGA NVVPLVASNR IGKEIIETEH GPSQITFYGT SFIAGPTGEI VAEADDKSEA VLVAQFDLDM IKSKRQSWGV FRDRRPDLYK VLLTMDGNL

Arabidopsis Description

CPAN-carbamoylputrescine amidase [Source:UniProtKB/Swiss-Prot;Acc:Q8VYF5]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.