Factsheet for Os05t0428600-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, extracellular

Predictor Summary:

plastid 2
endoplasmic reticulum 8
extracellular 4
vacuole 4
plasma membrane 4
golgi 4
nucleus 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular, plasma membrane
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	plastid
EpiLoc:	endoplasmic reticulum
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	endoplasmic reticulum, nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	endoplasmic reticulum

plasma membrane: 18260611
extracellular: 18372294

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

msms PMID: 18372294 doi

T Aki, M Shigyo, R Nakano, T Yoneyama, S Yanagisawa

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657 Japan.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os08t0197700-00	Rice	plasma membrane	95.05	85.47
TraesCS5D01G305500.1	Wheat	endoplasmic reticulum	74.09	83.72
TraesCS7D01G423100.1	Wheat	endoplasmic reticulum	80.35	83.64
TraesCS7B01G331400.1	Wheat	endoplasmic reticulum	79.77	83.03
TraesCS5B01G298000.1	Wheat	endoplasmic reticulum, nucleus, unclear	80.2	82.48
TraesCS5A01G298700.1	Wheat	endoplasmic reticulum	79.18	82.42
TraesCS7B01G330900.1	Wheat	endoplasmic reticulum	79.77	82.28
TraesCS7A01G430600.1	Wheat	endoplasmic reticulum	78.75	82.22
HORVU7Hr1G098810.1	Barley	endoplasmic reticulum	79.91	81.7
HORVU5Hr1G078400.1	Barley	plasma membrane	79.62	81.52
Os05t0367800-00	Rice	plasma membrane	79.04	81.17
EES01866	Sorghum	endoplasmic reticulum	78.17	80.51
Os02t0115900-02	Rice	plasma membrane	71.91	74.29
Os03t0710500-00	Rice	extracellular, plasma membrane	67.54	69.36
Os03t0821100-01	Rice	plasma membrane	57.35	60.71
Os03t0277300-01	Rice	plastid	57.64	60.64
Os01t0840100-01	Rice	nucleus, plasma membrane, plastid	56.77	60.19
Os03t0276500-01	Rice	plasma membrane	56.91	60.15
Os05t0460000-01	Rice	cytosol, nucleus, plastid	56.48	60.06
Os11t0703900-01	Rice	extracellular, plasma membrane	56.33	59.63
Os03t0276800-01	Rice	cytosol	45.41	55.71
Os11t0187600-01	Rice	cytosol	37.26	52.35
Os11t0187500-01	Rice	cytosol, mitochondrion, plastid	41.92	49.91
Os05t0303000-01	Rice	plastid	30.42	45.53
Os03t0113700-01	Rice	mitochondrion	43.81	44.53
Os02t0774300-01	Rice	mitochondrion	43.81	44.33
Os09t0491772-01	Rice	mitochondrion	43.52	43.71
Os12t0244100-01	Rice	plastid	43.81	43.12

Protein Annotations

Gene3D:1.20.1270.10	Gene3D:2.60.34.10	Gene3D:3.30.420.40	Gene3D:3.90.640.10	MapMan:35.1	EntrezGene:4338866
ProteinID:AAS90667.1	ProteinID:AAV59416.1	ProteinID:BAF17527.1	ProteinID:BAS94137.1	ncoils:Coil	GO:GO:0000166
GO:GO:0003674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005783	InterPro:HSP70_C_sf	InterPro:HSP70_peptide-bd_sf	InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam
InterPro:IPR029047	InterPro:IPR029048	EnsemblPlantsGene:Os05g0428600	EnsemblPlants:Os05t0428600-01	PFAM:PF00012	PRINTS:PR00301
ScanProsite:PS00297	ScanProsite:PS00329	ScanProsite:PS01036	PANTHER:PTHR19375	PANTHER:PTHR19375:SF317	UniProt:Q75HQ0
SUPFAM:SSF100920	SUPFAM:SSF100934	SUPFAM:SSF53067	SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI00003589E4
RefSeq:XP_015638801.1	SEG:seg	:	:	:	:

Description

ER chaperone BiP4, Binding protein 4, Mediator 37_5Similar to HSP70 precursor. (Os05t0428600-01)

Coordinates

chr5:-:21033769..21036041

Molecular Weight (calculated)

74316.9 Da

IEP (calculated)

4.847

GRAVY (calculated)

-0.379

Length

687 amino acids

Sequence
(BLAST)

001: MARPRRASTA TTMQLGLLLA ALLLFTSSLA GSVAAAAPPP PAGAKGGGAK SGGGGGTVIG IDLGTTYSCV GVYRNDRVEI IANDQGNRIT PSWVAFTDGG
101: ERLIGEAAKN QAAANPERTI YDAKRLIGRQ FSDAEVQRDM KLLPFAVVDR NGKPHVRVEV KDGDVRVFSP EEVSAMVLTR MKETAEAYLG EKVTRAVVTV
201: PAYFNDAQRQ ATKDAGVIAG LTVDRIINEP TAAAIAYGID KKGAEKNVLV FDLGGGTFDV SILAIDNGVF EVLATNGDTH LGGEDFDQRL MDHFVKVIRR
301: KHGRDIAGDA RALGKLRREC ERAKRALSNQ HQVRVEIESL FDGVDFSEPL SRARFEELNG DLFKKTMVPV RKAMADAGLG KGDIDEIVLV GGSTRIPKVQ
401: QLLKDYFGGK EPNRGVNPDE AVAYGAAVQA SIISGHVDEN TESMILLDVA PLTLGLETAG GVMTKLIPRN TVVPTKKTQV FTTYKDRQTT VTIQVFEGER
501: SMTRDNRLLG KFDLTGIAPA PRGAPQIAVT FEVDANGILS VLAADKATGR SEKITISGDD RKISQEEIDR MVREAEEFAD EDRRHREQVD ARNSLEAYVY
601: NVKSTLGGKM ADAMEGEEKE KVEEAVREAH EWLDGNPDAG KEEYEEKLRE LEDVCNPVMS AVYQRSGGGG GAPEDGNVDD EDDHDEL

Best Arabidopsis Sequence Match ( AT5G42020.1 )
(BLAST)

001: MARSFGANST VVLAIIFFGC LFAFSTAKEE ATKLGSVIGI DLGTTYSCVG VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD
101: VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN
201: VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV LSTNGDTHLG GEDFDHRIME YFIKLIKKKH QKDISKDNKA LGKLRRECER
301: AKRALSSQHQ VRVEIESLFD GVDLSEPLTR ARFEELNNDL FRKTMGPVKK AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV
401: AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLTGVPPAPR
501: GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVSDKDKL ADKLEGDEKE
601: KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP GAGGESSTEE EDESHDEL

Arabidopsis Description

BIPHeat shock protein 70 (Hsp 70) family protein [Source:TAIR;Acc:AT5G42020]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.