Factsheet for Os08t0197700-00

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 4
endoplasmic reticulum 2
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704
plasma membrane: 28056797

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 28056797 doi

N Yang, T Wang

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China., Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China. twang@ibcas.ac.cn.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Os05t0428600-01	Rice	extracellular, plasma membrane	85.47	95.05
TraesCS5D01G305500.1	Wheat	endoplasmic reticulum	66.75	83.88
TraesCS7D01G423100.1	Wheat	endoplasmic reticulum	71.99	83.33
TraesCS7B01G331400.1	Wheat	endoplasmic reticulum	71.73	83.03
TraesCS5B01G298000.1	Wheat	endoplasmic reticulum, nucleus, unclear	72.38	82.78
Os05t0367800-00	Rice	plasma membrane	72.38	82.66
TraesCS5A01G298700.1	Wheat	endoplasmic reticulum	71.34	82.58
TraesCS7B01G330900.1	Wheat	endoplasmic reticulum	71.86	82.43
TraesCS7A01G430600.1	Wheat	endoplasmic reticulum	70.81	82.22
HORVU7Hr1G098810.1	Barley	endoplasmic reticulum	72.25	82.14
HORVU5Hr1G078400.1	Barley	plasma membrane	71.86	81.82
EES01866	Sorghum	endoplasmic reticulum	69.5	79.61
Os02t0115900-02	Rice	plasma membrane	64.66	74.29
Os03t0710500-00	Rice	extracellular, plasma membrane	60.6	69.21
Os03t0821100-01	Rice	plasma membrane	50.92	59.94
Os03t0277300-01	Rice	plastid	51.18	59.88
Os03t0276500-01	Rice	plasma membrane	50.65	59.54
Os01t0840100-01	Rice	nucleus, plasma membrane, plastid	50.39	59.41
Os05t0460000-01	Rice	cytosol, nucleus, plastid	50.13	59.29
Os11t0703900-01	Rice	extracellular, plasma membrane	50.13	59.01
Os03t0276800-01	Rice	cytosol	40.18	54.82
Os11t0187600-01	Rice	cytosol	33.51	52.35
Os11t0187500-01	Rice	cytosol, mitochondrion, plastid	37.43	49.57
Os05t0303000-01	Rice	plastid	27.62	45.97
Os03t0113700-01	Rice	mitochondrion	39.66	44.82
Os02t0774300-01	Rice	mitochondrion	39.14	44.04
Os09t0491772-01	Rice	mitochondrion	39.14	43.71
Os12t0244100-01	Rice	plastid	39.53	43.27

Protein Annotations

Gene3D:1.20.1270.10	Gene3D:2.60.34.10	Gene3D:3.30.420.40	Gene3D:3.90.640.10	MapMan:35.1	ncoils:Coil
GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005515	GO:GO:0005524	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005737	GO:GO:0005783	InterPro:HSP70_C_sf	InterPro:HSP70_peptide-bd_sf
InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam	InterPro:IPR029047	InterPro:IPR029048	EnsemblPlantsGene:Os08g0197700	EnsemblPlants:Os08t0197700-00
PFAM:PF00012	PRINTS:PR00301	ScanProsite:PS00297	ScanProsite:PS00329	ScanProsite:PS01036	PANTHER:PTHR19375
PANTHER:PTHR19375:SF317	SUPFAM:SSF100920	SUPFAM:SSF100934	SUPFAM:SSF53067	UniParc:UPI000393A7D8	SEG:seg

Description

ER chaperone BiP5, Binding protein 5, Mediator 37_7Similar to Luminal binding protein 5 precursor (BiP 5) (78 kDa glucose-regulated protein homolog 5) (GRP 78-5). (Os08t0197700-00)

Coordinates

chr8:-:5645714..5648057

Molecular Weight (calculated)

83242.6 Da

IEP (calculated)

5.133

GRAVY (calculated)

-0.410

Length

764 amino acids

Sequence
(BLAST)

001: MYEDETESAP IRYARTATGT LSLTRLTLYP DRPIRSTNGP DHAADVYSLR LVGSGTHQIG VGLGLGRRTL YIYRDARIDT KPKAKTTIMA RPRRASTMQL
101: GLFLAALLLL TPSPAGSVAA AKGGGAKSGG GGTVIGIDLG TTYSCVGVYR NGHVEIIAND QGNRITPSWV AFTDGGERLI GEAAKNQAAA NPERTIYDAK
201: RLIGRQFSDA EVQRDMKLLP FAVVDRNGKP HVRVEVKDGD VRVFSPEEVS AMVLTRMKET AEAYLGEKVT RAVVTVPAYF NDAQRQATKD AGVIAGLTVD
301: RIINEPTAAA IAYGIDKKGA EKNVLVFDLG GGTFDVSILA IDNGVFEVLA TNGDTHLGGE DFDQRLMDHF VKVIRRKHGR DITGDARALG KLRRECERAK
401: RALSNQHQVR VEVESLFDGV DLSEPLSRAR FEELNSDLFK KTMVPVRKAM ADARLSKGDI DEIVLVGGST RIPKVQQLLK DYFGGKEPNR GVNPDEAVAY
501: GAAVQASIIS GHVDENTESM ILLDVAPLTL GLETAGGVMA KLIPRNTVVP TKKTQVFTTY KDKQTTVTIQ VFEGERSMTR DNRLLGRFDL AGIAPAPRGA
601: PQIEVTFEVD ANGILSVLAA DKATGRSEKI TISGDDRKIS QEEIDRMVRE AEEFAEEDRR HREQVDARNS LEAYVYNIKN TLGGKMADAM EGEEKDKVEE
701: AVREAYEWLD GNPDAGKEEY EEKLRELEDV CNPVMSAVYQ RSGGGGGGAP EDGNVDDEDD HDEL

Best Arabidopsis Sequence Match ( AT5G42020.3 )
(BLAST)

001: MCSLFLDLNV FVYNVVLQFH DLKGCLFAFS TAKEEATKLG SVIGIDLGTT YSCVGVYKNG HVEIIANDQG NRITPSWVGF TDSERLIGEA AKNQAAVNPE
101: RTVFDVKRLI GRKFEDKEVQ KDRKLVPYQI VNKDGKPYIQ VKIKDGETKV FSPEEISAMI LTKMKETAEA YLGKKIKDAV VTVPAYFNDA QRQATKDAGV
201: IAGLNVARII NEPTAAAIAY GLDKKGGEKN ILVFDLGGGT FDVSVLTIDN GVFEVLSTNG DTHLGGEDFD HRIMEYFIKL IKKKHQKDIS KDNKALGKLR
301: RECERAKRAL SSQHQVRVEI ESLFDGVDLS EPLTRARFEE LNNDLFRKTM GPVKKAMDDA GLQKSQIDEI VLVGGSTRIP KVQQLLKDFF EGKEPNKGVN
401: PDEAVAYGAA VQGGILSGEG GDETKDILLL DVAPLTLGIE TVGGVMTKLI PRNTVIPTKK SQVFTTYQDQ QTTVSIQVFE GERSLTKDCR LLGKFDLTGV
501: PPAPRGTPQI EVTFEVDANG ILNVKAEDKA SGKSEKITIT NEKGRLSQEE IDRMVKEAEE FAEEDKKVKE KIDARNALET YVYNMKNQVS DKDKLADKLE
601: GDEKEKIEAA TKEALEWLDE NQNSEKEEYD EKLKEVEAVC NPIITAVYQR SGGAPGAGGE SSTEEEDESH DEL

Arabidopsis Description

BIPHeat shock protein 70 (Hsp 70) family protein [Source:TAIR;Acc:AT5G42020]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.