Factsheet for Os03t0821100-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 4
plastid 1
mitochondrion 2
endoplasmic reticulum 1

Predictors

GFP

MS/MS

Papers

nucleus: 18203134
plasma membrane: 18260611
extracellular: 18372294
golgi: 25047511
plasma membrane: 27800704
plastid: 27992503

msms PMID: 18203134 doi

G Li, BR Nallamilli, F Tan, Z Peng

Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS 39762, USA.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 18260611 doi

SH Natera, KL Ford, AM Cassin, JH Patterson, EJ Newbigin, A Bacic

Plant Cell Biology Research Centre, VIC, Australia. s.natera@unimelb.edu.au

msms PMID: 18372294 doi

T Aki, M Shigyo, R Nakano, T Yoneyama, S Yanagisawa

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657 Japan.

msms PMID: 25047511 doi

Y Nie, F Huang, S Dong, L Li, P Gao, H Zhao, Y Wang, S Han

Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, College of Life Sciences, Beijing, P. R. China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
HORVU5Hr1G113180.1	Barley	cytosol	96.3	96.45
TraesCS5D01G492900.2	Wheat	cytosol	96.3	96.45
TraesCS5A01G479300.1	Wheat	golgi	96.3	96.45
TraesCS5B01G492500.1	Wheat	cytosol	96.15	96.3
Os11t0703900-01	Rice	extracellular, plasma membrane	94.92	94.92
VIT_06s0004g04510.t01	Wine grape	cytosol, extracellular	94.76	94.91
Os01t0840100-01	Rice	nucleus, plasma membrane, plastid	93.99	94.14
Solyc09g010630.2.1	Tomato	extracellular	93.84	93.84
PGSC0003DMT400023039	Potato	cytosol, extracellular	93.84	93.84
VIT_13s0019g01430.t01	Wine grape	cytosol	93.84	93.69
Os05t0460000-01	Rice	cytosol, nucleus, plastid	93.07	93.5
KRH19702	Soybean	endoplasmic reticulum	90.6	93.04
Os03t0276500-01	Rice	plasma membrane	93.07	92.92
GSMUA_Achr7P15160_001	Banana	cytosol	61.63	92.59
KRH67530	Soybean	endoplasmic reticulum, nucleus	92.6	92.04
KRG95806	Soybean	nucleus	92.6	91.9
Os03t0277300-01	Rice	plastid	89.52	88.97
Os03t0276800-01	Rice	cytosol	59.48	68.93
Os11t0187600-01	Rice	cytosol	48.84	64.83
Os02t0115900-02	Rice	plasma membrane	63.33	61.8
Os03t0710500-00	Rice	extracellular, plasma membrane	59.32	57.55
Os11t0187500-01	Rice	cytosol, mitochondrion, plastid	51.16	57.54
Os05t0428600-01	Rice	extracellular, plasma membrane	60.71	57.35
Os05t0367800-00	Rice	plasma membrane	55.62	53.96
Os08t0197700-00	Rice	plasma membrane	59.94	50.92
Os05t0303000-01	Rice	plastid	32.97	46.62
Os02t0774300-01	Rice	mitochondrion	48.23	46.1
Os03t0113700-01	Rice	mitochondrion	47.3	45.41
Os09t0491772-01	Rice	mitochondrion	46.84	44.44
Os12t0244100-01	Rice	plastid	46.69	43.41

Protein Annotations

Gene3D:1.20.1270.10	MapMan:19.1.5.1	Gene3D:2.60.34.10	Gene3D:3.30.30.30	Gene3D:3.30.420.40	Gene3D:3.90.640.10
EntrezGene:4334598	ProteinID:AAO65876.1	ProteinID:ABF99592.1	EMBL:AK069740	ProteinID:BAF13638.1	ProteinID:BAS87090.1
ncoils:Coil	ProteinID:EAZ29089.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005524
InterPro:HSP70_C_sf	InterPro:HSP70_peptide-bd_sf	InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam	InterPro:IPR029047	InterPro:IPR029048
EnsemblPlantsGene:Os03g0821100	EnsemblPlants:Os03t0821100-01	PFAM:PF00012	PRINTS:PR00301	ScanProsite:PS00297	ScanProsite:PS00329
ScanProsite:PS01036	PANTHER:PTHR19375	PANTHER:PTHR19375:SF327	UniProt:Q84TA1	SUPFAM:SSF100920	SUPFAM:SSF100934
SUPFAM:SSF53067	UniParc:UPI00000560EF	RefSeq:XP_015631367.1	SEG:seg	:	:

Description

heat shock protein 70, heat-shock protein 70, HEAT SHOCK PROTEIN 70KD, Mediator 37_4Similar to Non-cell-autonomous heat shock cognate protein 70. (Os03t0821100-01)

Coordinates

chr3:+:34446327..34450665

Molecular Weight (calculated)

71315.8 Da

IEP (calculated)

4.835

GRAVY (calculated)

-0.424

Length

649 amino acids

Sequence
(BLAST)

001: MAPTKGEGPA IGIDLGTTYS CVGVWQHDRV EIIANDQGNR TTPSYVGFTD TERLIGDAAK NQVAMNPINT VFDAKRLIGR RFSDASVQSD IKMWPYKVIP
101: GPGDKPMIVV QYKGEEKQFS AEEISSMVLI KMREIAEAYL GSTVKNAVVT VPAYFNDSQR QATKDAGVIA GLNVMRIINE PTAAAIAYGL DKKATSVGEK
201: NVLIFDLGGG TFDVSLLTIE EGIFEVKATA GDTHLGGEDF DNRLVNHFVQ EFKRKHKKDI SGNPRALRRL RTSCERAKRT LSSTAQTTIE IDSLFEGVDF
301: YSTITRARFE ELNMDLFRKC MEPVEKCLRD AKMDKSTVHD VVLVGGSTRI PRVQQLLQDF FNGKELCKSI NPDEAVAYGA AVQAAILSGE GNEKVQDLLL
401: LDVTPLSLGL ETAGGVMTVL IPRNTTIPTK KEQVFSTYSD NQPGVLIQVY EGERTRTRDN NLLGKFELSG IPPAPRGVPQ ITVCFDIDAN GILNVSAEDK
501: TTGQKNKITI TNDKGRLSKE EIEKMVQDAE KYKSEDEEHK KKVDAKNSLE NYAYNMRNTI QDEKIASKLP ADDKKKIEDA VEQAIQWLDN NQLAEVEEFE
601: DKMKELEGLC NPIIAKMYQG AGADMGGGMD DDAPAAGGSG AGPKIEEVD

Best Arabidopsis Sequence Match ( AT5G02500.1 )
(BLAST)

001: MSGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS ERLIGDAAKN QVAMNPVNTV FDAKRLIGRR FSDSSVQSDM KLWPFKIQAG
101: PADKPMIYVE YKGEEKEFAA EEISSMVLIK MREIAEAYLG VTIKNAVVTV PAYFNDSQRQ ATKDAGVIAG LNVMRIINEP TAAAIAYGLD KKATSVGEKN
201: VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE FKRKSKKDIT GNPRALRRLR TSCERAKRTL SSTAQTTIEI DSLYEGIDFY
301: STITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSTVHDV VLVGGSTRIP KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQGAILSGEG NEKVQDLLLL
401: DVTPLSLGLE TAGGVMTTLI PRNTTIPTKK EQVFSTYSDN QPGVLIQVYE GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
501: TGQKNKITIT NDKGRLSKDE IEKMVQEAEK YKSEDEEHKK KVEAKNALEN YAYNMRNTIQ DEKIGEKLPA ADKKKIEDSI EQAIQWLEGN QLAEADEFED
601: KMKELESICN PIIAKMYQGA GGEAGGPGAS GMDDDAPPAS GGAGPKIEEV D

Arabidopsis Description

MED37EProbable mediator of RNA polymerase II transcription subunit 37e [Source:UniProtKB/Swiss-Prot;Acc:P22953]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.