Factsheet for Os03t0710500-00

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, extracellular

Predictor Summary:

extracellular 5
endoplasmic reticulum 9
vacuole 5
plasma membrane 5
golgi 5
nucleus 1
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	extracellular, plasma membrane
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	secretory
EpiLoc:	endoplasmic reticulum
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	endoplasmic reticulum, nucleus
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	endoplasmic reticulum

nucleus: 18203134
plastid: 21433289
extracellular: 24083427
plasma membrane: 27800704

msms PMID: 18203134 doi

G Li, BR Nallamilli, F Tan, Z Peng

Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS 39762, USA.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 21433289 doi

S Reiland, J Grossmann, K Baerenfaller, P Gehrig, A Nunes-Nesi, AR Fernie, W Gruissem, S Baginsky

Department of Biology, Plant Biotechnology, ETH Zurich, Zurich, Switzerland.

msms PMID: 24083427 doi

T Shinano, T Yoshimura, T Watanabe, Y Unno, M Osaki, Y Nanjo, S Komatsu

Agricultural Radiation Research Center, NARO Tohoku Agricultural Research Center , 50, Harajyukuminami, Arai, Fukushima 960-2156, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os05t0156500-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT1G09080.1	Os05t0156500-01	AT3G62600.1	19763086

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER93587	Sorghum	endoplasmic reticulum	87.89	86.98
VIT_14s0060g02340.t01	Wine grape	endoplasmic reticulum	72.8	75.04
CDY22648	Canola	cytosol	66.82	73.88
Os02t0115900-02	Rice	plasma membrane	71.6	72.03
Solyc01g099660.2.1	Tomato	extracellular	71.9	71.9
PGSC0003DMT400063543	Potato	endoplasmic reticulum	71.6	71.6
AT1G09080.1	Thale cress	endoplasmic reticulum	71.9	71.26
Bra031657.1-P	Field mustard	endoplasmic reticulum	70.85	70.43
CDY06347	Canola	endoplasmic reticulum	67.12	69.94
Os05t0428600-01	Rice	extracellular, plasma membrane	69.36	67.54
Os05t0367800-00	Rice	plasma membrane	65.92	65.92
Os08t0197700-00	Rice	plasma membrane	69.21	60.6
Os03t0821100-01	Rice	plasma membrane	57.55	59.32
Os05t0460000-01	Rice	cytosol, nucleus, plastid	57.25	59.29
Os03t0277300-01	Rice	plastid	57.85	59.26
Os01t0840100-01	Rice	nucleus, plasma membrane, plastid	57.25	59.1
Os03t0276500-01	Rice	plasma membrane	57.25	58.92
Os11t0703900-01	Rice	extracellular, plasma membrane	56.2	57.94
Os03t0276800-01	Rice	cytosol	47.23	56.43
Os11t0187600-01	Rice	cytosol	39.46	53.99
Os11t0187500-01	Rice	cytosol, mitochondrion, plastid	42.75	49.57
Os03t0113700-01	Rice	mitochondrion	44.99	44.53
Os05t0303000-01	Rice	plastid	30.49	44.44
Os02t0774300-01	Rice	mitochondrion	44.39	43.74
Os12t0244100-01	Rice	plastid	44.39	42.55
Os09t0491772-01	Rice	mitochondrion	43.2	42.25

Protein Annotations

Gene3D:1.20.1270.10	Gene3D:2.60.34.10	Gene3D:3.30.420.40	Gene3D:3.90.640.10	MapMan:35.1	ProteinID:AAX95660.1
ProteinID:ABF98501.1	ProteinID:BAF12962.1	ProteinID:BAS86012.1	GO:GO:0000003	GO:GO:0000166	GO:GO:0003674
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623	GO:GO:0005634
GO:GO:0005654	GO:GO:0005737	GO:GO:0005783	GO:GO:0008150	GO:GO:0009653	GO:GO:0009856
GO:GO:0009860	GO:GO:0009987	GO:GO:0016043	GO:GO:0016049	GO:GO:0016592	GO:GO:0030154
GO:GO:0040007	InterPro:HSP70_C_sf	InterPro:HSP70_peptide-bd_sf	InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam	InterPro:IPR029047
InterPro:IPR029048	EnsemblPlantsGene:Os03g0710500	EnsemblPlants:Os03t0710500-00	PFAM:PF00012	PRINTS:PR00301	ScanProsite:PS00297
ScanProsite:PS00329	ScanProsite:PS01036	PANTHER:PTHR19375	PANTHER:PTHR19375:SF215	UniProt:Q53RJ5	SUPFAM:SSF100920
SUPFAM:SSF100934	SUPFAM:SSF53067	SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI000009C88B	SEG:seg

Description

ER chaperone BiP2, Binding protein 2Similar to Luminal binding protein. (Os03t0710500-00)

Coordinates

chr3:+:28647086..28649570

Molecular Weight (calculated)

73378.3 Da

IEP (calculated)

5.169

GRAVY (calculated)

-0.408

Length

669 amino acids

Sequence
(BLAST)

001: MARDKQSALI VAAFVLLCSG CLCGVADGAK GGRKTKGPVI GIDLGTTYSC VGVYRNGHVD IVANDQGNRI TPSWVAFTDD ERLVGEAAKN QAALNPDRTI
101: FDIKRLIGRR FDDEEVQRDV KYLPYKVVDK GGKPYVEVRV KAGEVKVFSP EEISAMILAK MKETAESYLG QRVTDAVVTV PAYFNDAQRQ ATKDAGTIAG
201: LNVPRIINEP TAAAIAYGLD RKGAGEMTNV LVYDLGGGTF DVSVLSLDHG VFEVLATSGD THLGGEDFDR RVMDHFIRLV KRQHGRDIGG DGRALGKLRR
301: ECERAKRALS RQHQVRVEIE ALFVGVDFSE TLTRAKFEEL NMDLFKKTLG PVRKAIADAK LKKSDIDEIV LVGGSTRIPK VQELLKEMFD GKEPTKGINP
401: DEAVAYGAAV QGSIISGEGG AETKDILLLD VTPLTLGIET AGGVMTKLIP RNTRIPVKKS QVFTTYEDHQ TTVSIKVFEG ERSLTKDCRE LGRFDLSGIA
501: PAPRGVPQIE VTFEVDENGI LHVTASDKAA GRSKSITITN DKGRLSQEEI DRMVREAEEF AEEDRRVRER VDARNRLENY VYRMRSAVRD GGMAGKIGDD
601: DRERMESALT EALEWLEDND GGARTAEKED YEEKLKEVEQ VCGPIIKQVY KKSGDASAGA GDDDDVNEL

Best Arabidopsis Sequence Match ( AT5G28540.1 )
(BLAST)

001: MARSFGANST VVLAIIFFGC LFALSSAIEE ATKLGSVIGI DLGTTYSCVG VYKNGHVEII ANDQGNRITP SWVGFTDSER LIGEAAKNQA AVNPERTVFD
101: VKRLIGRKFE DKEVQKDRKL VPYQIVNKDG KPYIQVKIKD GETKVFSPEE ISAMILTKMK ETAEAYLGKK IKDAVVTVPA YFNDAQRQAT KDAGVIAGLN
201: VARIINEPTA AAIAYGLDKK GGEKNILVFD LGGGTFDVSV LTIDNGVFEV LSTNGDTHLG GEDFDHRVME YFIKLIKKKH QKDISKDNKA LGKLRRECER
301: AKRALSSQHQ VRVEIESLFD GVDFSEPLTR ARFEELNNDL FRKTMGPVKK AMDDAGLQKS QIDEIVLVGG STRIPKVQQL LKDFFEGKEP NKGVNPDEAV
401: AYGAAVQGGI LSGEGGDETK DILLLDVAPL TLGIETVGGV MTKLIPRNTV IPTKKSQVFT TYQDQQTTVS IQVFEGERSL TKDCRLLGKF DLNGIPPAPR
501: GTPQIEVTFE VDANGILNVK AEDKASGKSE KITITNEKGR LSQEEIDRMV KEAEEFAEED KKVKEKIDAR NALETYVYNM KNQVNDKDKL ADKLEGDEKE
601: KIEAATKEAL EWLDENQNSE KEEYDEKLKE VEAVCNPIIT AVYQRSGGAP GGAGGESSTE EEDESHDEL

Arabidopsis Description

MED37AMediator of RNA polymerase II transcription subunit 37a [Source:UniProtKB/Swiss-Prot;Acc:Q9LKR3]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.