Factsheet for Os03t0276500-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 4
plastid 1
mitochondrion 2
endoplasmic reticulum 1

Predictors

GFP

MS/MS

Papers

nucleus: 18203134
plasma membrane: 18557835
plasma membrane: 19502382
golgi: 25047511
plasma membrane: 27800704
plastid: 27992503

msms PMID: 18203134 doi

G Li, BR Nallamilli, F Tan, Z Peng

Department of Biochemistry and Molecular Biology, Mississippi State University, Mississippi State, MS 39762, USA.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 19502382 doi

M Fujiwara, S Hamada, M Hiratsuka, Y Fukao, T Kawasaki, K Shimamoto

Laboratory of Plant Protein Analysis, Plant Education Unit, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.

msms PMID: 18557835 doi

SA Whiteman, TS Nühse, DA Ashford, D Sanders, FJ Maathuis

Department of Biology Area 9, University of York, York YO10 5DD, UK.

msms PMID: 27992503 doi

S Xing, X Meng, L Zhou, H Mujahid, C Zhao, Y Zhang, C Wang, Z Peng

Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Starkville, Mississippi, United States of America., Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing, Jiangsu, China.

msms PMID: 25047511 doi

Y Nie, F Huang, S Dong, L Li, P Gao, H Zhao, Y Wang, S Han

Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal University, College of Life Sciences, Beijing, P. R. China.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os01t0624500-01
Os03t0854500-01

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G12580.1	Os01t0624500-01	AT4G11260.1	18065690
AT3G12580.1	Os03t0854500-01	AT4G17750.1	11807141

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS4D01G207500.1	Wheat	cytosol	96.77	96.62
TraesCS4B01G206700.1	Wheat	cytosol	96.77	96.62
HORVU4Hr1G059390.1	Barley	cytosol	96.62	96.47
TraesCS4A01G097900.2	Wheat	cytosol	96.62	95.88
EER95095	Sorghum	cytosol	94.62	94.76
Os01t0840100-01	Rice	nucleus, plasma membrane, plastid	94.15	94.44
Zm00001d028630_P005	Maize	cytosol, extracellular, nucleus, plasma membrane, plastid	94.0	94.14
Os11t0703900-01	Rice	extracellular, plasma membrane	93.85	93.99
Os05t0460000-01	Rice	cytosol, nucleus, plastid	93.23	93.81
Bra001457.1-P	Field mustard	cytosol	84.77	93.71
KRH01631	Soybean	endoplasmic reticulum	92.92	93.07
Os03t0821100-01	Rice	plasma membrane	92.92	93.07
AT3G12580.1	Thale cress	cytosol	92.46	92.46
CDX73815	Canola	cytosol	92.62	92.33
KRH70484	Soybean	cytosol	79.54	92.32
CDY00784	Canola	cytosol	92.31	92.31
CDY50531	Canola	cytosol	92.31	92.31
CDY52661	Canola	cytosol	91.85	92.27
Bra038734.1-P	Field mustard	cytosol	92.15	92.15
Os03t0277300-01	Rice	plastid	89.23	88.82
Os03t0276800-01	Rice	cytosol	59.85	69.46
Os11t0187600-01	Rice	cytosol	48.77	64.83
Os02t0115900-02	Rice	plasma membrane	63.54	62.11
Os11t0187500-01	Rice	cytosol, mitochondrion, plastid	51.23	57.71
Os03t0710500-00	Rice	extracellular, plasma membrane	58.92	57.25
Os05t0428600-01	Rice	extracellular, plasma membrane	60.15	56.91
Os05t0367800-00	Rice	plasma membrane	55.38	53.81
Os08t0197700-00	Rice	plasma membrane	59.54	50.65
Os05t0303000-01	Rice	plastid	32.77	46.41
Os02t0774300-01	Rice	mitochondrion	48.31	46.24
Os03t0113700-01	Rice	mitochondrion	47.23	45.41
Os09t0491772-01	Rice	mitochondrion	46.62	44.3
Os12t0244100-01	Rice	plastid	46.46	43.27

Protein Annotations

Gene3D:1.20.1270.10	MapMan:19.1.5.1	Gene3D:2.60.34.10	Gene3D:3.30.30.30	Gene3D:3.30.420.40	Gene3D:3.90.640.10
EntrezGene:4332413	ProteinID:ABF95258.1	EMBL:AK072830	ProteinID:BAF11626.1	ProteinID:BAS83517.1	ncoils:Coil
ProteinID:EAZ26443.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005524	EMBL:GU120344
InterPro:HSP70_C_sf	InterPro:HSP70_peptide-bd_sf	InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam	InterPro:IPR029047	InterPro:IPR029048
EnsemblPlantsGene:Os03g0276500	EnsemblPlants:Os03t0276500-01	PFAM:PF00012	PRINTS:PR00301	ScanProsite:PS00297	ScanProsite:PS00329
ScanProsite:PS01036	PANTHER:PTHR19375	PANTHER:PTHR19375:SF243	UniProt:Q10NA9	SUPFAM:SSF100920	SUPFAM:SSF100934
SUPFAM:SSF53067	UniParc:UPI0000DB41AE	RefSeq:XP_015630538.1	SEG:seg	:	:

Description

71.1 KDA CLASS I HEAT SHOCK PROTEINSimilar to Heat shock protein 70. (Os03t0276500-01)

Coordinates

chr3:+:9370160..9373067

Molecular Weight (calculated)

71104.3 Da

IEP (calculated)

4.834

GRAVY (calculated)

-0.453

Length

650 amino acids

Sequence
(BLAST)

001: MAGKGDGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS ERLIGDAAKN QVAMNPINTV FDAKRLIGRR FSDTSVQSDA KLWPFKVLPG
101: PGDKPMIGVQ YKGEEKQFSA EEISSMVLNK MKETAEAYLG STVKNAVVTV PAYFNDSQRQ ATKDAGVISG LNVMRIINEP TAAAIAYGLD KKSSSVGEKN
201: VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE FKRKNKKDIT GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLYEGIDFY
301: TTITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSTVHDV VLVGGSTRIP RVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILTGEG NEKVQDLLLL
401: DVTPLSQGLE TAGGVMTVLI PRNTTIPTKK EQVFSTYSDN QPGVLIQVYE GERTRTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
501: TGQKNKITIT NDKGRLSKED IEKMVQEAEK YKAEDEEHKK KVDAKNSLEN YAYNMRNTIK DDKIASKLPE ADKKKIEDAI DGAISWLDSN QLAEAEEFED
601: KMKELEGVCN PIIAKMYQGA GADMAGGMDE DAPAAAGGSS GPGPKIEEVD

Best Arabidopsis Sequence Match ( AT3G12580.1 )
(BLAST)

001: MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS ERLIGDAAKN QVAMNPTNTV FDAKRLIGRR YSDPSVQADK SHWPFKVVSG
101: PGEKPMIVVN HKGEEKQFSA EEISSMVLIK MREIAEAFLG SPVKNAVVTV PAYFNDSQRQ ATKDAGVISG LNVMRIINEP TAAAIAYGLD KKASSVGEKN
201: VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE FKRKNKKDIT GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLFEGIDFY
301: TTITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSSVHDV VLVGGSTRIP KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
401: DVTPLSLGLE TAGGVMTVLI PRNTTIPTKK EQIFSTYSDN QPGVLIQVYE GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
501: TGQKNKITIT NDKGRLSKEE IEKMVQEAEK YKAEDEEHKK KVDAKNALEN YAYNMRNTIK DEKIASKLDA ADKKKIEDAI DQAIEWLDGN QLAEADEFED
601: KMKELESLCN PIIARMYQGA GPDMGGAGGM DDDTPAGGSG GAGPKIEEVD

Arabidopsis Description

MED37CProbable mediator of RNA polymerase II transcription subunit 37c [Source:UniProtKB/Swiss-Prot;Acc:Q9LHA8]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.