Factsheet for Zm00001d028630_P005

Subcellular Localization

min:

: max

Winner_takes_all: extracellular, plastid, cytosol, plasma membrane, nucleus

Predictor Summary:

cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	cytosol, extracellular, nucleus, plasma membrane, plastid
Any Predictor:	cytosol, mitochondrion
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

extracellular: 20408568
plastid: 22065420
plasma membrane: 23353019
nucleus: 24677780

msms PMID: 24677780 doi

B Guo, Y Chen, C Li, T Wang, R Wang, B Wang, S Hu, X Du, H Xing, X Song, Y Yao, Q Sun, Z Ni

State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China; National Plant Gene Research Centre (Beijing), Beijing, China.

msms PMID: 23353019 doi

D Hopff, S Wienkoop, S Lüthje

University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Plant Physiology, Ohnhorststraße 18, D-22609 Hamburg, Germany.

msms PMID: 20408568 doi

W Ma, N Muthreich, C Liao, M Franz-Wachtel, W Schütz, F Zhang, F Hochholdinger, C Li

Department of Plant Nutrition, China Agricultural University, Beijing, PR China.

msms PMID: 22065420 doi

W Majeran, G Friso, Y Asakura, X Qu, M Huang, L Ponnala, KP Watkins, A Barkan, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d011401_P001
Zm00001d012823_P001
Zm00001d034886_P001
Zm00001d044172_P002

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G12580.1	Zm00001d011401_P001	AT4G11260.1	18065690
AT3G12580.1	Zm00001d044172_P002	AT4G11260.1	18065690
AT3G12580.1	Zm00001d012823_P001	AT4G17750.1	11807141
AT3G12580.1	Zm00001d034886_P001	AT4G17750.1	11807141

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
EER95095	Sorghum	cytosol	98.77	98.77
Os03t0276500-01	Rice	plasma membrane	94.14	94.0
HORVU4Hr1G059390.1	Barley	cytosol	94.14	93.86
TraesCS4B01G206700.1	Wheat	cytosol	93.68	93.39
TraesCS4D01G207500.1	Wheat	cytosol	93.53	93.24
TraesCS4A01G097900.2	Wheat	cytosol	93.37	92.52
Bra001457.1-P	Field mustard	cytosol	83.36	92.01
Zm00001d042922_P002	Maize	cytosol	91.83	91.98
Zm00001d010529_P001	Maize	cytosol	91.83	91.83
KRH01631	Soybean	endoplasmic reticulum	91.68	91.68
Zm00001d012420_P001	Maize	plasma membrane	91.37	91.51
Zm00001d041550_P005	Maize	cytosol	91.22	91.22
Zm00001d030725_P003	Maize	plasma membrane	91.06	91.06
KRH70484	Soybean	cytosol	78.12	90.54
CDY50531	Canola	cytosol	90.6	90.46
CDY00784	Canola	cytosol	90.6	90.46
CDY52661	Canola	cytosol	90.14	90.42
CDX73815	Canola	cytosol	90.75	90.34
AT3G12580.1	Thale cress	cytosol	90.45	90.31
Bra038734.1-P	Field mustard	cytosol	90.29	90.15
Zm00001d047799_P002	Maize	cytosol, extracellular, plasma membrane, plastid	87.98	87.58
Zm00001d048687_P002	Maize	cytosol, extracellular, plasma membrane	59.32	74.76
Zm00001d028639_P001	Maize	cytosol	55.47	66.67
Zm00001d054043_P007	Maize	endoplasmic reticulum	62.71	61.39
Zm00001d014993_P001	Maize	endoplasmic reticulum	62.56	61.24
Zm00001d013842_P003	Maize	mitochondrion	46.53	44.54
Zm00001d000295_P001	Maize	mitochondrion, plasma membrane	45.92	43.95
Zm00001d033591_P001	Maize	plastid	46.23	43.92
Zm00001d013507_P001	Maize	plastid	46.23	43.92
Zm00001d006036_P001	Maize	mitochondrion	45.61	43.47
Zm00001d023802_P001	Maize	plastid	45.92	42.39
Zm00001d041119_P001	Maize	plastid	45.76	42.25

Protein Annotations

Gene3D:1.20.1270.10	EntrezGene:100281806	MapMan:19.1.5.1	Gene3D:2.60.34.10	Gene3D:3.30.30.30	Gene3D:3.30.420.40
Gene3D:3.90.640.10	UniProt:B6SZ69	ncoils:Coil	EMBL:EU958034	GO:GO:0000166	GO:GO:0003674
GO:GO:0005488	GO:GO:0005524	InterPro:HSP70_C_sf	InterPro:HSP70_peptide-bd_sf	InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam
InterPro:IPR029047	InterPro:IPR029048	ProteinID:ONL96608.1	ProteinID:ONL96611.1	PFAM:PF00012	PRINTS:PR00301
ScanProsite:PS00297	ScanProsite:PS00329	ScanProsite:PS01036	PANTHER:PTHR19375	PANTHER:PTHR19375:SF243	SUPFAM:SSF100920
SUPFAM:SSF100934	SUPFAM:SSF53067	UniParc:UPI000182C43D	EnsemblPlantsGene:Zm00001d028630	EnsemblPlants:Zm00001d028630_P005	EnsemblPlants:Zm00001d028630_T005
SEG:seg	:	:	:	:	:

Description

Heat shock cognate 70 kDa protein 2

Coordinates

chr1:+:41454277..41456420

Molecular Weight (calculated)

71142.3 Da

IEP (calculated)

4.795

GRAVY (calculated)

-0.455

Length

649 amino acids

Sequence
(BLAST)

001: MAGKGDGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS ERLVGDAAKN QVAMNPINTV FDAKRLIGRR FSDASVQSDA KLWPFKVIPG
101: SGDKPMIGVQ FRGEEKQFSA EEISSMILNK MKETAEAYLG TTIKNAVVTV PAYFNDSQRQ ATKDAGVISG LNVMRIINEP TAAAIAYGLD KKSSSDGEKN
201: VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRLVNHFVQE FKRKNKKDIT GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLYEGIDFY
301: TTITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSSVHDV VLVGGSTRIP RVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILTGEG NEKVQDLLLL
401: DVSPLSLGLE TAGGVMTVLI PRNTTIPTKK EQVFSTYSDN QPGVLIQVYE GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDMDANG ILNVSAEDKT
501: TGQKNKITIT NDKGRLSKEE IEKMVQEAEK YKAEDEEHQK KVNAKNTLEN YAYNMRNTIR DDKIASKLPE DDKKKIDDAV EGAINWLDNN QLAEVDEFED
601: KMKELEGICN PIIAKMYQGA AGPDMAGGMD QDASPAGGSG AGPKIEEVD

Best Arabidopsis Sequence Match ( AT3G09440.1 )
(BLAST)

001: MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS ERLIGDAAKN QVAMNPINTV FDAKRLIGRR FTDSSVQSDI KLWPFTLKSG
101: PAEKPMIVVN YKGEDKEFSA EEISSMILIK MREIAEAYLG TTIKNAVVTV PAYFNDSQRQ ATKDAGVIAG LNVMRIINEP TAAAIAYGLD KKATSVGEKN
201: VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE FKRKNKKDIS GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLFDGIDFY
301: APITRARFEE LNIDLFRKCM EPVEKCLRDA KMDKNSIDDV VLVGGSTRIP KVQQLLVDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
401: DVTPLSLGLE TAGGVMTVLI QRNTTIPTKK EQVFSTYSDN QPGVLIQVYE GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
501: TGQKNKITIT NDKGRLSKDE IEKMVQEAEK YKSEDEEHKK KVDAKNALEN YAYNMRNTIR DEKIGEKLAG DDKKKIEDSI EAAIEWLEAN QLAECDEFED
601: KMKELESICN PIIAKMYQGG EAGGPAAGGM DEDVPPSAGG AGPKIEEVD

Arabidopsis Description

HSP70-3Heat shock protein 70 (Hsp 70) family protein [Source:UniProtKB/TrEMBL;Acc:A0A178VI76]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.