Factsheet for Zm00001d012420_P001

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

cytosol 3
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion
MultiLoc:	cytosol
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

plasma membrane: 18521681
extracellular: 20408568
plastid: 22065420
plastid: 23198870
plasma membrane: 23353019
plasma membrane: 23508561
mitochondrion: 27297264
plasma membrane: 27341663
cytosol: 28250383

msms PMID: 28250383 doi

CN Meisrimler, F Buck, S Lüthje

University Hospital Eppendorf, Institute of Clinical Chemistry, Campus Science, Martinistraße 52, D-20246 Hamburg, Germany. buck@uke.de., University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Oxidative Stress and Plant Proteomics Group, Ohnhorststraße 18, D-22609 Hamburg, Germany. c_m_2406@yahoo.de., University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Oxidative Stress and Plant Proteomics Group, Ohnhorststraße 18, D-22609 Hamburg, Germany. s.luthje@botanik.uni-hamburg.de.

msms PMID: 23353019 doi

D Hopff, S Wienkoop, S Lüthje

University of Hamburg, Biocenter Klein Flottbek and Botanical Garden, Plant Physiology, Ohnhorststraße 18, D-22609 Hamburg, Germany.

msms PMID: 27297264 doi

D Dahal, KJ Newton, BP Mooney

Division of Biological Sciences, ‡Department of Biochemistry, and §The Charles W Gehrke Proteomics Center, University of Missouri , Columbia, Missouri 65211, United States.

msms PMID: 23198870 doi

M Huang, G Friso, K Nishimura, X Qu, PD Olinares, W Majeran, Q Sun, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, United States.

msms PMID: 18521681 doi

P Carletti, A Masi, B Spolaore, P Polverino De Laureto, M De Zorzi, L Turetta, M Ferretti, S Nardi

Department of Agricultural Biotechnology, University of Padua, Agripolis, Strada Romea, 16-35020 Legnaro, Padova, Italy.

msms PMID: 27341663 doi

P Voothuluru, JC Anderson, RE Sharp, SC Peck

Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA., Division of Biochemistry, University of Missouri, Columbia, MO, 65211, USA., Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA., Interdisciplinary Plant Group, University of Missouri, Columbia, MO, 65211, USA.

msms PMID: 20408568 doi

W Ma, N Muthreich, C Liao, M Franz-Wachtel, W Schütz, F Zhang, F Hochholdinger, C Li

Department of Plant Nutrition, China Agricultural University, Beijing, PR China.

msms PMID: 22065420 doi

W Majeran, G Friso, Y Asakura, X Qu, M Huang, L Ponnala, KP Watkins, A Barkan, KJ van Wijk

Department of Plant Biology, Cornell University, Ithaca, New York 14853, USA.

msms PMID: 23508561 doi

Z Zhang, P Voothuluru, M Yamaguchi, RE Sharp, SC Peck

Division of Biochemistry, University of Missouri Columbia, MO, USA ; Christopher S. Bond Life Sciences Center, University of Missouri Columbia, MO, USA ; Interdisciplinary Plant Group, University of Missouri Columbia, MO, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Zm00001d011401_P001
Zm00001d012823_P001
Zm00001d034886_P001
Zm00001d044172_P002

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G12580.1	Zm00001d011401_P001	AT4G11260.1	18065690
AT3G12580.1	Zm00001d044172_P002	AT4G11260.1	18065690
AT3G12580.1	Zm00001d012823_P001	AT4G17750.1	11807141
AT3G12580.1	Zm00001d034886_P001	AT4G17750.1	11807141

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d042922_P002	Maize	cytosol	97.99	97.99
KXG26133	Sorghum	cytosol	91.82	96.59
Zm00001d010529_P001	Maize	cytosol	95.83	95.69
TraesCS3D01G351900.1	Wheat	cytosol	95.37	94.79
TraesCS3D01G352400.2	Wheat	cytosol, golgi	94.6	94.75
TraesCS3B01G390700.1	Wheat	cytosol	94.91	94.33
TraesCS3B01G390800.1	Wheat	cytosol	89.97	94.03
HORVU1Hr1G072220.4	Barley	cytosol	85.34	93.89
TraesCS1B01G294300.1	Wheat	cytosol, golgi, unclear	93.21	93.21
TraesCS1D01G284000.2	Wheat	cytosol, golgi	93.06	93.06
TraesCS1A01G285000.2	Wheat	cytosol, golgi	92.9	92.9
KRH70484	Soybean	cytosol	80.25	92.86
Zm00001d041550_P005	Maize	cytosol	92.59	92.45
KRH01631	Soybean	endoplasmic reticulum	92.59	92.45
Bra001457.1-P	Field mustard	cytosol	83.8	92.35
AT3G12580.1	Thale cress	cytosol	92.59	92.31
Zm00001d030725_P003	Maize	plasma membrane	92.44	92.3
CDY52661	Canola	cytosol	91.98	92.12
CDX73815	Canola	cytosol	92.44	91.87
CDY50531	Canola	cytosol	92.13	91.85
CDY00784	Canola	cytosol	92.13	91.85
Bra038734.1-P	Field mustard	cytosol	92.13	91.85
Zm00001d028630_P005	Maize	cytosol, extracellular, nucleus, plasma membrane, plastid	91.51	91.37
Zm00001d047799_P002	Maize	cytosol, extracellular, plasma membrane, plastid	91.67	91.1
HORVU3Hr1G086500.3	Barley	cytosol, plastid	95.52	87.8
Zm00001d048687_P002	Maize	cytosol, extracellular, plasma membrane	60.19	75.73
Zm00001d028639_P001	Maize	cytosol	56.48	67.78
Zm00001d054043_P007	Maize	endoplasmic reticulum	64.35	62.9
Zm00001d014993_P001	Maize	endoplasmic reticulum	64.35	62.9
Zm00001d013842_P003	Maize	mitochondrion	48.3	46.17
Zm00001d000295_P001	Maize	mitochondrion, plasma membrane	47.53	45.43
Zm00001d006036_P001	Maize	mitochondrion	47.38	45.08
Zm00001d013507_P001	Maize	plastid	47.07	44.66
Zm00001d033591_P001	Maize	plastid	46.91	44.51
Zm00001d023802_P001	Maize	plastid	46.76	43.1
Zm00001d041119_P001	Maize	plastid	46.76	43.1

Protein Annotations

Gene3D:1.20.1270.10	EntrezGene:100501536	MapMan:19.1.5.1	Gene3D:2.60.34.10	Gene3D:3.30.30.30	Gene3D:3.30.420.40
Gene3D:3.90.640.10	ProteinID:AQK99505.1	ProteinID:AQK99508.1	ProteinID:AQK99510.1	EMBL:BT085557	UniProt:C4J410
ncoils:Coil	GO:GO:0000166	GO:GO:0003674	GO:GO:0005488	GO:GO:0005524	InterPro:HSP70_C_sf
InterPro:HSP70_peptide-bd_sf	InterPro:Heat_shock_70_CS	InterPro:Hsp_70_fam	InterPro:IPR029047	InterPro:IPR029048	PFAM:PF00012
PRINTS:PR00301	ScanProsite:PS00297	ScanProsite:PS00329	ScanProsite:PS01036	PANTHER:PTHR19375	PANTHER:PTHR19375:SF323
SUPFAM:SSF100920	SUPFAM:SSF100934	SUPFAM:SSF53067	UniParc:UPI000189D312	EnsemblPlantsGene:Zm00001d012420	EnsemblPlants:Zm00001d012420_P001
EnsemblPlants:Zm00001d012420_T001	SEG:seg	:	:	:	:

Description

heat shock protein1Heat shock 70 kDa protein

Coordinates

chr8:-:174179964..174183186

Molecular Weight (calculated)

70886.4 Da

IEP (calculated)

4.804

GRAVY (calculated)

-0.398

Length

648 amino acids

Sequence
(BLAST)

001: MAKSEGPAIG IDLGTTYSCV GVWQHDRVEI IANDQGNRTT PSYVGFTDTE RLIGDAAKNQ VAMNPTNTVF DAKRLIGRRF SDPSVQSDMK LWPFKVIPGP
101: GDKPMIVVNY KGEEKQFAAE EISSMVLIKM KEIAEAYLGS TIKNAVVTVP AYFNDSQRQA TKDAGVIAGL NVMRIINEPT AAAIAYGLDK KATSSGEKNV
201: LIFDLGGGTF DVSLLTIEEG IFEVKATAGD THLGGEDFDN RMVNHFVQEF KRKNKKDISG NPRALRRLRT ACERAKRTLS STAQTTIEID SLFEGIDFYS
301: TITRARFEEL NMDLFRKCME PVEKCLRDAK MDKSSVHDVV LVGGSTRIPK VQQLLQDFFN GKELCKSINP DEAVAYGAAV QAAILSGEGN EKVQDLLLLD
401: VTPLSLGLET AGGVMTVLIP RNTTIPTKKE QVFSTYSDNQ PGVLIQVYEG ERARTKDNNL LGKFELSGIP PAPRGVPQIT VTFDIDANGI LNVSAEDKTT
501: GQKNKITITN DKGRLSKEEI EKMVQEAEKY KAEDEEVKKK VDAKNALENY AYNMRNTIKD DKIASKLPAE DKKKIEDAVD GAISWLDSNQ LAEVEEFEDK
601: MKELEGICNP IIAKMYQGEG AGMGAAAGMD EDAPSGGSGA GPKIEEVD

Best Arabidopsis Sequence Match ( AT3G09440.1 )
(BLAST)

001: MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS ERLIGDAAKN QVAMNPINTV FDAKRLIGRR FTDSSVQSDI KLWPFTLKSG
101: PAEKPMIVVN YKGEDKEFSA EEISSMILIK MREIAEAYLG TTIKNAVVTV PAYFNDSQRQ ATKDAGVIAG LNVMRIINEP TAAAIAYGLD KKATSVGEKN
201: VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE FKRKNKKDIS GNPRALRRLR TACERAKRTL SSTAQTTIEI DSLFDGIDFY
301: APITRARFEE LNIDLFRKCM EPVEKCLRDA KMDKNSIDDV VLVGGSTRIP KVQQLLVDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
401: DVTPLSLGLE TAGGVMTVLI QRNTTIPTKK EQVFSTYSDN QPGVLIQVYE GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
501: TGQKNKITIT NDKGRLSKDE IEKMVQEAEK YKSEDEEHKK KVDAKNALEN YAYNMRNTIR DEKIGEKLAG DDKKKIEDSI EAAIEWLEAN QLAECDEFED
601: KMKELESICN PIIAKMYQGG EAGGPAAGGM DEDVPPSAGG AGPKIEEVD

Arabidopsis Description

HSP70-3Heat shock protein 70 (Hsp 70) family protein [Source:UniProtKB/TrEMBL;Acc:A0A178VI76]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.