Subcellular Localization
min:
: max
Winner_takes_all: cytosol
Predictor Summary:
Predictor Summary:
- nucleus 1
- mitochondrion 1
- cytosol 3
- plasma membrane 2
- extracellular 1
- endoplasmic reticulum 1
- vacuole 1
- golgi 1
Predictors | GFP | MS/MS | Papers |
---|---|---|---|
PPI
No PPI Data
Homology
Paralog
locus | Identity | Homology Identity |
---|
Ortholog
locus | Homology Species | Location | Identity | Homology Identity |
---|---|---|---|---|
PGSC0003DMT400019683 | Potato | cytosol, mitochondrion | 78.77 | 62.35 |
PGSC0003DMT400005041 | Potato | cytosol | 75.19 | 48.51 |
PGSC0003DMT400005733 | Potato | cytosol | 62.66 | 46.58 |
PGSC0003DMT400046497 | Potato | cytosol | 37.08 | 44.48 |
PGSC0003DMT400005040 | Potato | cytosol, nucleus, peroxisome | 62.15 | 42.41 |
PGSC0003DMT400078332 | Potato | cytosol | 49.1 | 42.2 |
PGSC0003DMT400005032 | Potato | cytosol | 61.89 | 41.37 |
PGSC0003DMT400005052 | Potato | plastid | 72.89 | 38.46 |
PGSC0003DMT400005013 | Potato | plastid | 63.43 | 28.15 |
PGSC0003DMT400005017 | Potato | cytosol, plastid | 63.43 | 28.09 |
PGSC0003DMT400005006 | Potato | vacuole | 60.1 | 26.37 |
PGSC0003DMT400051415 | Potato | cytosol | 27.88 | 22.52 |
PGSC0003DMT400078333 | Potato | cytosol, vacuole | 49.36 | 22.29 |
PGSC0003DMT400078334 | Potato | cytosol, plastid | 51.92 | 22.07 |
PGSC0003DMT400002788 | Potato | cytosol | 47.57 | 21.86 |
PGSC0003DMT400012479 | Potato | cytosol | 34.02 | 20.21 |
PGSC0003DMT400062766 | Potato | cytosol | 27.11 | 19.7 |
PGSC0003DMT400031120 | Potato | cytosol | 13.81 | 17.65 |
PGSC0003DMT400059836 | Potato | cytosol | 17.39 | 17.39 |
PGSC0003DMT400069012 | Potato | cytosol | 24.3 | 16.99 |
PGSC0003DMT400093366 | Potato | cytosol | 29.92 | 16.16 |
PGSC0003DMT400025432 | Potato | cytosol | 28.13 | 16.13 |
PGSC0003DMT400047508 | Potato | cytosol | 15.86 | 15.54 |
PGSC0003DMT400012486 | Potato | cytosol | 34.27 | 15.33 |
PGSC0003DMT400062180 | Potato | cytosol | 31.46 | 14.59 |
PGSC0003DMT400062184 | Potato | cytosol | 32.23 | 14.52 |
PGSC0003DMT400017538 | Potato | nucleus | 32.23 | 14.19 |
PGSC0003DMT400076082 | Potato | cytosol | 23.79 | 13.96 |
PGSC0003DMT400050509 | Potato | cytosol | 31.2 | 13.93 |
PGSC0003DMT400036070 | Potato | cytosol, plastid | 32.74 | 13.82 |
PGSC0003DMT400010966 | Potato | cytosol | 28.39 | 13.42 |
PGSC0003DMT400060798 | Potato | cytosol | 27.62 | 13.28 |
PGSC0003DMT400050821 | Potato | cytosol | 29.92 | 13.24 |
PGSC0003DMT400019329 | Potato | cytosol | 24.81 | 13.22 |
PGSC0003DMT400050805 | Potato | cytosol | 29.67 | 13.11 |
PGSC0003DMT400005011 | Potato | cytosol, plastid | 19.69 | 13.01 |
GSMUA_Achr7P20240_001 | Banana | mitochondrion | 23.02 | 12.88 |
PGSC0003DMT400034063 | Potato | cytosol | 26.34 | 12.75 |
TraesCS7B01G501200.1 | Wheat | cytosol | 17.9 | 12.73 |
VIT_16s0022g01330.t01 | Wine grape | cytosol | 20.46 | 12.64 |
PGSC0003DMT400042194 | Potato | cytosol | 30.18 | 12.54 |
PGSC0003DMT400019300 | Potato | cytosol, plasma membrane, plastid | 21.74 | 12.48 |
PGSC0003DMT400020346 | Potato | cytosol | 14.07 | 12.47 |
PGSC0003DMT400047502 | Potato | cytosol | 29.92 | 12.3 |
PGSC0003DMT400056264 | Potato | endoplasmic reticulum | 27.37 | 12.06 |
PGSC0003DMT400047510 | Potato | nucleus | 30.95 | 11.91 |
PGSC0003DMT400020350 | Potato | cytosol, nucleus, plasma membrane | 25.83 | 11.57 |
PGSC0003DMT400012121 | Potato | extracellular, golgi | 16.11 | 11.56 |
PGSC0003DMT400080647 | Potato | cytosol | 26.6 | 11.52 |
PGSC0003DMT400030040 | Potato | cytosol | 17.9 | 11.51 |
VIT_15s0045g01020.t01 | Wine grape | cytosol | 18.93 | 11.51 |
PGSC0003DMT400020353 | Potato | cytosol, nucleus, plastid | 26.6 | 11.5 |
PGSC0003DMT400088449 | Potato | cytosol | 26.6 | 11.43 |
TraesCS6A01G404000.1 | Wheat | cytosol | 15.86 | 11.33 |
PGSC0003DMT400066996 | Potato | cytosol | 25.58 | 11.29 |
PGSC0003DMT400020342 | Potato | cytosol | 26.09 | 11.26 |
PGSC0003DMT400007570 | Potato | cytosol | 26.09 | 11.23 |
PGSC0003DMT400030826 | Potato | cytosol | 13.55 | 11.23 |
PGSC0003DMT400058642 | Potato | cytosol | 13.04 | 11.21 |
PGSC0003DMT400019304 | Potato | cytosol | 25.06 | 11.15 |
PGSC0003DMT400025440 | Potato | cytosol, mitochondrion | 13.3 | 11.13 |
PGSC0003DMT400056267 | Potato | cytosol | 12.79 | 10.96 |
PGSC0003DMT400066993 | Potato | cytosol, plasma membrane | 25.32 | 10.86 |
PGSC0003DMT400075885 | Potato | cytosol, plastid | 24.55 | 10.82 |
PGSC0003DMT400041023 | Potato | cytosol | 22.76 | 10.63 |
PGSC0003DMT400020349 | Potato | cytosol | 25.83 | 10.63 |
TraesCS6D01G387400.1 | Wheat | cytosol | 15.35 | 10.51 |
PGSC0003DMT400020360 | Potato | cytosol | 25.32 | 10.38 |
PGSC0003DMT400019301 | Potato | cytosol | 21.74 | 10.04 |
TraesCS2B01G507000.1 | Wheat | cytosol | 18.67 | 9.99 |
PGSC0003DMT400010970 | Potato | plasma membrane | 20.46 | 9.71 |
GSMUA_Achr7P20230_001 | Banana | cytosol | 21.99 | 9.68 |
PGSC0003DMT400050826 | Potato | cytosol | 21.99 | 9.47 |
GSMUA_Achr7P20220_001 | Banana | cytosol | 21.74 | 9.45 |
VIT_15s0046g03660.t01 | Wine grape | cytosol | 19.95 | 9.27 |
PGSC0003DMT400005012 | Potato | cytosol | 8.18 | 9.25 |
PGSC0003DMT400031116 | Potato | cytosol | 18.41 | 9.24 |
PGSC0003DMT400030019 | Potato | cytosol | 19.95 | 9.24 |
KRH01534 | Soybean | cytosol | 19.69 | 9.21 |
PGSC0003DMT400030011 | Potato | cytosol | 19.44 | 9.03 |
VIT_15s0045g00810.t01 | Wine grape | cytosol | 9.72 | 8.92 |
PGSC0003DMT400027529 | Potato | cytosol | 19.95 | 8.83 |
KRG88521 | Soybean | cytosol | 19.44 | 8.82 |
GSMUA_AchrUn_... | Banana | cytosol | 16.88 | 8.8 |
GSMUA_AchrUn_... | Banana | cytosol | 6.14 | 8.76 |
VIT_15s0046g02750.t01 | Wine grape | cytosol | 20.72 | 8.65 |
VIT_15s0046g02820.t01 | Wine grape | cytosol | 20.72 | 8.58 |
PGSC0003DMT400016712 | Potato | cytosol | 18.41 | 8.57 |
KRH17117 | Soybean | cytosol | 19.18 | 8.55 |
VIT_15s0046g02730.t01 | Wine grape | cytosol | 19.95 | 8.48 |
VIT_15s0046g02810.t01 | Wine grape | cytosol | 20.2 | 8.43 |
KRH23932 | Soybean | cytosol | 19.95 | 8.4 |
PGSC0003DMT400031142 | Potato | cytosol | 17.9 | 8.39 |
KRH45220 | Soybean | cytosol, extracellular, plastid | 19.18 | 8.39 |
VIT_00s0448g00020.t01 | Wine grape | cytosol | 20.2 | 8.38 |
PGSC0003DMT400021403 | Potato | cytosol | 20.2 | 8.32 |
VIT_15s0045g00640.t01 | Wine grape | cytosol | 10.23 | 8.28 |
PGSC0003DMT400016707 | Potato | cytosol | 17.9 | 8.24 |
TraesCS7A01G561700.1 | Wheat | cytosol | 18.16 | 8.18 |
PGSC0003DMT400075439 | Potato | cytosol | 19.95 | 8.16 |
KRH01595 | Soybean | cytosol | 18.67 | 8.15 |
TraesCS7B01G423500.1 | Wheat | cytosol | 17.9 | 8.08 |
HORVU1Hr1G075170.1 | Barley | cytosol | 17.9 | 8.05 |
PGSC0003DMT400030042 | Potato | cytosol | 17.65 | 8.02 |
PGSC0003DMT400031132 | Potato | cytosol | 16.37 | 8.0 |
TraesCS6B01G448100.1 | Wheat | cytosol | 16.11 | 7.99 |
HORVU5Hr1G072540.26 | Barley | cytosol | 17.9 | 7.96 |
Zm00001d045512_P001 | Maize | cytosol, mitochondrion | 16.88 | 7.92 |
KRH01598 | Soybean | cytosol | 17.9 | 7.92 |
TraesCS6B01G447900.1 | Wheat | cytosol | 14.83 | 7.91 |
TraesCS7B01G501000.1 | Wheat | cytosol | 17.65 | 7.89 |
TraesCS7B01G500700.1 | Wheat | cytosol | 17.14 | 7.89 |
PGSC0003DMT400030044 | Potato | mitochondrion | 17.14 | 7.87 |
TraesCS6D01G387500.1 | Wheat | cytosol, plastid | 15.86 | 7.84 |
TraesCS5A01G261700.1 | Wheat | cytosol | 17.65 | 7.83 |
TraesCS1D01G324700.2 | Wheat | cytosol | 17.39 | 7.82 |
TraesCS1A01G323600.1 | Wheat | cytosol | 17.39 | 7.81 |
TraesCS2B01G507100.1 | Wheat | cytosol | 18.93 | 7.8 |
PGSC0003DMT400030034 | Potato | cytosol | 16.62 | 7.75 |
TraesCS2B01G151600.2 | Wheat | cytosol | 16.62 | 7.67 |
TraesCS7A01G562000.1 | Wheat | cytosol | 17.14 | 7.66 |
TraesCS7B01G500900.1 | Wheat | cytosol | 17.14 | 7.66 |
Os03t0709200-01 | Rice | cytosol | 18.67 | 7.61 |
TraesCS6B01G448300.2 | Wheat | cytosol, peroxisome, plasma membrane | 15.35 | 7.59 |
TraesCS2D01G481300.1 | Wheat | cytosol | 18.41 | 7.52 |
TraesCS2D01G510300.1 | Wheat | cytosol | 10.49 | 7.5 |
TraesCS5A01G261600.1 | Wheat | cytosol | 16.88 | 7.49 |
HORVU2Hr1G022620.8 | Barley | cytosol | 16.62 | 7.46 |
TraesCS7B01G501900.1 | Wheat | cytosol | 16.62 | 7.46 |
VIT_15s0045g00880.t01 | Wine grape | cytosol | 14.32 | 7.42 |
TraesCS7A01G561900.4 | Wheat | cytosol | 17.39 | 7.41 |
TraesCS2D01G510000.1 | Wheat | cytosol | 14.58 | 7.4 |
OQU82378 | Sorghum | nucleus | 15.86 | 7.4 |
TraesCS7B01G449900.2 | Wheat | cytosol | 16.62 | 7.34 |
TraesCS6A01G403900.1 | Wheat | plasma membrane, vacuole | 14.07 | 7.27 |
TraesCS7B01G501100.1 | Wheat | cytosol | 16.11 | 7.23 |
TraesCS1B01G336700.1 | Wheat | cytosol | 14.83 | 7.23 |
Os10t0183000-01 | Rice | plasma membrane | 16.37 | 7.16 |
TraesCS7D01G548100.1 | Wheat | cytosol | 16.11 | 7.09 |
TraesCS5B01G259900.1 | Wheat | cytosol | 16.62 | 7.0 |
TraesCS2A01G459100.1 | Wheat | plastid | 13.55 | 6.67 |
TraesCS6B01G448400.1 | Wheat | cytosol | 14.32 | 6.6 |
PGSC0003DMT400030030 | Potato | nucleus | 11.76 | 6.26 |
VIT_15s0046g02800.t01 | Wine grape | cytosol | 10.49 | 5.98 |
VIT_15s0045g00680.t01 | Wine grape | cytosol | 11.76 | 5.95 |
TraesCS2A01G477200.1 | Wheat | cytosol | 10.23 | 5.92 |
TraesCS7B01G500800.1 | Wheat | cytosol | 10.49 | 5.69 |
PGSC0003DMT400056268 | Potato | cytosol | 8.18 | 5.21 |
TraesCS5D01G269100.1 | Wheat | cytosol | 8.7 | 4.93 |
PGSC0003DMT400025439 | Potato | cytosol | 6.91 | 4.91 |
VIT_15s0045g00920.t01 | Wine grape | cytosol | 6.39 | 4.81 |
VIT_15s0045g00980.t01 | Wine grape | cytosol | 7.16 | 4.69 |
PGSC0003DMT400030031 | Potato | cytosol | 3.84 | 4.67 |
PGSC0003DMT400030022 | Potato | cytosol | 5.63 | 4.2 |
PGSC0003DMT400005732 | Potato | cytosol, endoplasmic reticulum, extracellular, golgi, plasma membrane, vacuole | 2.56 | 3.58 |
PGSC0003DMT400036058 | Potato | cytosol | 5.63 | 2.87 |
PGSC0003DMT400062366 | Potato | cytosol | 2.3 | 2.41 |
TraesCS2B01G536900.1 | Wheat | cytosol | 3.07 | 2.17 |
PGSC0003DMT400082505 | Potato | cytosol | 2.3 | 2.04 |
Os11t0633500-01 | Rice | cytosol | 2.56 | 1.89 |
PGSC0003DMT400019678 | Potato | cytosol, extracellular | 1.53 | 1.89 |
TraesCS6B01G447800.1 | Wheat | cytosol | 2.56 | 1.88 |
PGSC0003DMT400082452 | Potato | cytosol | 2.3 | 1.79 |
PGSC0003DMT400062272 | Potato | cytosol | 2.05 | 1.58 |
PGSC0003DMT400062762 | Potato | plastid | 1.53 | 1.52 |
PGSC0003DMT400046494 | Potato | cytosol | 1.02 | 1.46 |
TraesCS7A01G562500.1 | Wheat | cytosol | 2.05 | 1.44 |
KRG88520 | Soybean | plastid | 0.77 | 0.86 |
PGSC0003DMT400019327 | Potato | cytosol, extracellular | 0.77 | 0.62 |
PGSC0003DMT400036104 | Potato | extracellular, plasma membrane | 0.26 | 0.29 |
PGSC0003DMT400058647 | Potato | cytosol, nucleus | 0.26 | 0.16 |
PGSC0003DMT400005034 | Potato | plastid | 0.0 | 0.0 |
PGSC0003DMT400005046 | Potato | cytosol, extracellular | 0.0 | 0.0 |
PGSC0003DMT400010987 | Potato | cytosol | 0.0 | 0.0 |
PGSC0003DMT400027536 | Potato | cytosol | 0.0 | 0.0 |
PGSC0003DMT400048669 | Potato | cytosol | 0.0 | 0.0 |
PGSC0003DMT400059833 | Potato | cytosol | 0.0 | 0.0 |
PGSC0003DMT400068337 | Potato | extracellular | 0.0 | 0.0 |
PGSC0003DMT400082507 | Potato | cytosol | 0.0 | 0.0 |
TraesCS2A01G129300.1 | Wheat | cytosol | 0.0 | 0.0 |
TraesCS2D01G510100.1 | Wheat | cytosol | 0.0 | 0.0 |
TraesCS6B01G448000.1 | Wheat | cytosol | 0.0 | 0.0 |
TraesCS7B01G501300.1 | Wheat | cytosol | 0.0 | 0.0 |
VIT_15s0021g00130.t01 | Wine grape | cytosol, nucleus, plastid | 0.0 | 0.0 |
VIT_15s0045g01010.t01 | Wine grape | cytosol | 0.0 | 0.0 |
Protein Annotations
EnsemblPlants:PGSC0003DMT400094032 | EnsemblPlantsGene:PGSC0003DMG400043603 | Gene3D:3.80.10.10 | InterPro:IPR032675 | InterPro:LRR_dom_sf | PANTHER:PTHR43886 |
PANTHER:PTHR43886:SF17 | PGSC:PGSC0003DMG400043603 | SEG:seg | SUPFAM:SSF52047 | UniParc:UPI000296179A | UniProt:M1DT88 |
MapMan:35.1 | : | : | : | : | : |
Description
Disease resistance protein BS2 [Source:PGSC_GENE;Acc:PGSC0003DMG400043603]
Coordinates
chr11:-:5856359..5857534
Molecular Weight (calculated)
45385.7 Da
IEP (calculated)
6.195
GRAVY (calculated)
-0.035
Length
391 amino acids
Sequence
(BLAST)
(BLAST)
001: MKTCKIHDLL RQLCLSKAHT ENVMHVMNGN DLEAIDNQRR MILLSEVEEK YDYGPRNSSG IVRTFISMGV VALPKEMCSI VSEFKLLKVL DVLSVLWYDF
101: SSVIPELVHL RYVSARIEEA PSLAKLRSLQ TIILRRYFSG SVRPITLELQ PLDIWRMSEI RHMDIDWPLY ISNLVEAEQT LFLNNLQTLY LYNSPCVAEI
201: IRRTPNLKKL KILYRSKLPD SLSILQDLET LHIKEQENID PMIISRDISL PNLKKLKLFR TRIPWEVVNL LANLTNLEVL NGNRAFVGTD WKVDEDVVFH
301: KLKYLRLYVC FDLERWELAA GSDNFPMLEQ LKLYVLPKLE EIPESIGEIM TLKSIRIVDC SSGVNTSARK IQEEQESLGN YELQFEIVLV C
101: SSVIPELVHL RYVSARIEEA PSLAKLRSLQ TIILRRYFSG SVRPITLELQ PLDIWRMSEI RHMDIDWPLY ISNLVEAEQT LFLNNLQTLY LYNSPCVAEI
201: IRRTPNLKKL KILYRSKLPD SLSILQDLET LHIKEQENID PMIISRDISL PNLKKLKLFR TRIPWEVVNL LANLTNLEVL NGNRAFVGTD WKVDEDVVFH
301: KLKYLRLYVC FDLERWELAA GSDNFPMLEQ LKLYVLPKLE EIPESIGEIM TLKSIRIVDC SSGVNTSARK IQEEQESLGN YELQFEIVLV C
001: MQDLYMVDSI VSFGVEKLWK LLSQEYERFQ GVEEQITELR DDLKMLMAFL SDADAKKQTR ALARNCLEEI KEITYDAEDI IEIFLLKGSV NMRSLACFPG
101: GRREIALQIT SISKRISKVI QVMQNLGIKS DIMDGVDSHA QLERKRELRH TFSSESESNL VGLEKNVEKL VEELVGNDSS HGVSITGLGG LGKTTLARQI
201: FDHDKVKSHF DGLAWVCVSQ EFTRKDVWKT ILGNLSPKYK DSDLPEDDIQ KKLFQLLETK KALIVFDDLW KREDWYRIAP MFPERKAGWK VLLTSRNDAI
301: HPHCVTFKPE LLTHDECWKL LQRIAFSKQK TITGYIIDKE MVKMAKEMTK HCKRLPLAVK LLGGLLDAKH TLRQWKLISE NIISHIVVGG TSSNENDSSS
401: VNHVLSLSFE GLPGYLKHCL LYLASYPEDH EIEIERLSYV WAAEGITYPG NYEGATIRDV ADLYIEELVK RNMVISERDA LTSRFEKCQL HDLMREICLL
501: KAKEENFLQI VTDPTSSSSV HSLASSRSRR LVVYNTSIFS GENDMKNSKL RSLLFIPVGY SRFSMGSNFI ELPLLRVLDL DGAKFKGGKL PSSIGKLIHL
601: KYLSLYQASV TYLPSSLRNL KSLLYLNLRI NSGQLINVPN VFKEMLELRY LSLPWERSSL TKLELGNLLK LETLINFSTK DSSVTDLHRM TKLRTLQILI
701: SGEGLHMETL SSALSMLGHL EDLTVTPSEN SVQFKHPKLI YRPMLPDVQH FPSHLTTISL VYCFLEEDPM PTLEKLLQLK VVSLWYNAYV GRRMVCTGGG
801: FPPLHRLEIW GLDALEEWIV EEGSMPLLHT LHIVDCKKLK EIPDGLRFIS SLKELAIRTN EKVFQKKVSK GGEDYYKMQH VPLIRYNWPQ EPENNEVIYS
901: FPSPII
101: GRREIALQIT SISKRISKVI QVMQNLGIKS DIMDGVDSHA QLERKRELRH TFSSESESNL VGLEKNVEKL VEELVGNDSS HGVSITGLGG LGKTTLARQI
201: FDHDKVKSHF DGLAWVCVSQ EFTRKDVWKT ILGNLSPKYK DSDLPEDDIQ KKLFQLLETK KALIVFDDLW KREDWYRIAP MFPERKAGWK VLLTSRNDAI
301: HPHCVTFKPE LLTHDECWKL LQRIAFSKQK TITGYIIDKE MVKMAKEMTK HCKRLPLAVK LLGGLLDAKH TLRQWKLISE NIISHIVVGG TSSNENDSSS
401: VNHVLSLSFE GLPGYLKHCL LYLASYPEDH EIEIERLSYV WAAEGITYPG NYEGATIRDV ADLYIEELVK RNMVISERDA LTSRFEKCQL HDLMREICLL
501: KAKEENFLQI VTDPTSSSSV HSLASSRSRR LVVYNTSIFS GENDMKNSKL RSLLFIPVGY SRFSMGSNFI ELPLLRVLDL DGAKFKGGKL PSSIGKLIHL
601: KYLSLYQASV TYLPSSLRNL KSLLYLNLRI NSGQLINVPN VFKEMLELRY LSLPWERSSL TKLELGNLLK LETLINFSTK DSSVTDLHRM TKLRTLQILI
701: SGEGLHMETL SSALSMLGHL EDLTVTPSEN SVQFKHPKLI YRPMLPDVQH FPSHLTTISL VYCFLEEDPM PTLEKLLQLK VVSLWYNAYV GRRMVCTGGG
801: FPPLHRLEIW GLDALEEWIV EEGSMPLLHT LHIVDCKKLK EIPDGLRFIS SLKELAIRTN EKVFQKKVSK GGEDYYKMQH VPLIRYNWPQ EPENNEVIYS
901: FPSPII
Arabidopsis Description
Putative disease resistance protein At1g59780 [Source:UniProtKB/Swiss-Prot;Acc:Q9XIF0]
SUBAcon: [cytosol]
SUBAcon: [cytosol]
Hydropathy Plot
About CropPAL
The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.