Factsheet for Bra025145.1-P

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

nucleus 1
cytosol 1
mitochondrion 1
plasma membrane 4
extracellular 2
endoplasmic reticulum 2
vacuole 3
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, mitochondrion, nucleus, secretory
BaCelLo:	nucleus
EpiLoc:	cytosol
iPSORT:	mitochondrion
MultiLoc:	plasma membrane
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	vacuole
YLoc:	plasma membrane

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
CDY48073	Canola	plasma membrane	99.44	83.44
CDX83534	Canola	plasma membrane	97.21	81.56
AT3G25560.3	Thale cress	plasma membrane	88.27	73.26
Bra008659.1-P	Field mustard	plasma membrane	70.2	59.46
EER88222	Sorghum	plasma membrane	67.97	58.03
TraesCS6A01G289500.1	Wheat	plasma membrane	67.41	58.01
EES05592	Sorghum	plasma membrane	67.6	57.99
Zm00001d017913_P001	Maize	plasma membrane	67.78	57.96
TraesCS7D01G212800.1	Wheat	plasma membrane	67.41	57.92
TraesCS6B01G319700.1	Wheat	plasma membrane	67.41	57.92
TraesCS6D01G269400.1	Wheat	plasma membrane	67.41	57.83
TraesCS7B01G118100.1	Wheat	plasma membrane	67.23	57.76
TraesCS7A01G211100.1	Wheat	plasma membrane	67.23	57.76
HORVU7Hr1G043030.2	Barley	plasma membrane	66.67	57.28
Zm00001d037297_P002	Maize	plasma membrane	67.41	55.02
Os06t0274500-01	Rice	extracellular	65.36	54.84
Bra031479.1-P	Field mustard	endoplasmic reticulum, plasma membrane	55.49	54.78
HORVU6Hr1G072620.29	Barley	plasma membrane	67.23	52.32
Bra006335.1-P	Field mustard	plasma membrane	66.11	52.28
Bra032128.1-P	Field mustard	cytosol	46.74	51.54
Bra024069.1-P	Field mustard	plasma membrane	60.15	50.79
Bra011439.1-P	Field mustard	plasma membrane	48.79	50.38
Bra037006.1-P	Field mustard	mitochondrion	44.88	50.31
Bra041056.1-P	Field mustard	plasma membrane	48.23	50.1
Bra003911.1-P	Field mustard	plasma membrane	53.26	50.09
Bra034562.1-P	Field mustard	plasma membrane	52.51	49.82
Bra032133.1-P	Field mustard	plasma membrane	59.03	49.76
Zm00001d045728_P003	Maize	plasma membrane	67.04	49.45
Bra032125.1-P	Field mustard	plasma membrane	48.6	48.6
Bra024369.1-P	Field mustard	plasma membrane	48.98	46.8
Bra025056.1-P	Field mustard	plasma membrane	52.89	46.18
Bra016112.1-P	Field mustard	plasma membrane	50.47	44.65
Zm00001d018347_P001	Maize	cytosol	6.52	43.75
Bra028592.1-P	Field mustard	plasma membrane	49.35	43.37
Bra011200.1-P	Field mustard	plastid	43.2	43.28
Bra013136.1-P	Field mustard	plasma membrane	46.0	42.73
Bra038656.1-P	Field mustard	cytosol	45.44	42.14
Bra035044.1-P	Field mustard	plasma membrane, vacuole	23.28	35.61
Os02t0728500-01	Rice	extracellular, plasma membrane	20.48	33.95
Bra010115.1-P	Field mustard	plasma membrane	32.96	30.31
Bra005319.1-P	Field mustard	plasma membrane	27.0	29.59
Bra035884.1-P	Field mustard	plasma membrane	32.59	29.07
Bra023017.1-P	Field mustard	plasma membrane	27.56	27.46
Bra017307.1-P	Field mustard	plasma membrane	29.24	26.7

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.1.2	Gene3D:3.30.200.20	Gene3D:3.80.10.10	EnsemblPlantsGene:Bra025145	EnsemblPlants:Bra025145.1
EnsemblPlants:Bra025145.1-P	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674
GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0006464	GO:GO:0006468	GO:GO:0008150
GO:GO:0008152	GO:GO:0009987	GO:GO:0016020	GO:GO:0016021	GO:GO:0016301	GO:GO:0016310
GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	InterPro:IPR032675	InterPro:Kinase-like_dom_sf	InterPro:LRR_N_plant-typ
InterPro:LRR_dom_sf	UniProt:M4E8N9	PFAM:PF00069	PFAM:PF08263	ScanProsite:PS00107	ScanProsite:PS00108
PFscan:PS50011	PANTHER:PTHR27001	PANTHER:PTHR27001:SF217	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220
SUPFAM:SSF52058	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI000253F78D

Description

AT3G25560 (E=2e-215) NIK2 | NIK2 (NSP-INTERACTING KINASE 2); ATP binding / protein binding / protein kinase/ protein serine/threonine kinase

Coordinates

chrA06:+:23233049..23236851

Molecular Weight (calculated)

60168.4 Da

IEP (calculated)

7.066

GRAVY (calculated)

-0.133

Length

537 amino acids

Sequence
(BLAST)

001: MVQRKREVRE SYALACSSLF FFIALLCSSS AELTDKGVNY EVLALIGIKS SLIDPHGVLM NWDDTAVDPC SWNMITCSAD GFVLSLLLQN NYITGHIPPE
101: IGKLMKLKTL DLSTNNFTGQ IPSTLSASTN LQYFFLVYEA SDGGTKNRKI AIAFGAGLTC ICLLIIAFGF LIWWRRRHNQ QIFFDVNDQN KEEVCLGNLR
201: RFSFKELQSA TSNFSSKNLV GKGGFGNVYK GRLHDGTIVA VKRLKDVNNG GGEIQFQTEV EMISLAVHRN LLRLYGLCTT SMERLLVYPY MSNGSVASRL
301: RAKPVLDWST RKRIALGAAR GLLYLHEQCD PKIIHRDVKA ANILLDDYYE AVVGDFGLAK LLDHEESHVT TAVRGTVGHI APEYLSTGQS SEKTDVFGFG
401: ILLLELITGL RALEFGKAAN QRGAILDWVK KLQQEKNLEQ IIDKDLERNY DRIEVEEIVK VALLCTQYLP IHRPKMSEVV RMLEGDGLVE KWEASSQRTE
501: TNRSYSKPNE FSSSERYSDL TDDSSVLVQA MELSGPR

Best Arabidopsis Sequence Match ( AT3G25560.1 )
(BLAST)

001: MLQGRREAKK SYALFSSTFF FFFICFLSSS SAELTDKGVN FEVVALIGIK SSLTDPHGVL MNWDDTAVDP CSWNMITCSD GFVIRLEAPS QNLSGTLSSS
101: IGNLTNLQTV LLQNNYITGN IPHEIGKLMK LKTLDLSTNN FTGQIPFTLS YSKNLQYLRV NNNSLTGTIP SSLANMTQLT FLDLSYNNLS GPVPRSLAKT
201: FNVMGNSQIC PTGTEKDCNG TQPKPMSITL NSSQNKSSDG GTKNRKIAVV FGVSLTCVCL LIIGFGFLLW WRRRHNKQVL FFDINEQNKE EMCLGNLRRF
301: NFKELQSATS NFSSKNLVGK GGFGNVYKGC LHDGSIIAVK RLKDINNGGG EVQFQTELEM ISLAVHRNLL RLYGFCTTSS ERLLVYPYMS NGSVASRLKA
401: KPVLDWGTRK RIALGAGRGL LYLHEQCDPK IIHRDVKAAN ILLDDYFEAV VGDFGLAKLL DHEESHVTTA VRGTVGHIAP EYLSTGQSSE KTDVFGFGIL
501: LLELITGLRA LEFGKAANQR GAILDWVKKL QQEKKLEQIV DKDLKSNYDR IEVEEMVQVA LLCTQYLPIH RPKMSEVVRM LEGDGLVEKW EASSQRAETN
601: RSYSKPNEFS SSERYSDLTD DSSVLVQAME LSGPR

Arabidopsis Description

NIK2NSP-interacting kinase 2 [Source:UniProtKB/TrEMBL;Acc:F4JA15]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.