CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

cytosol 4
plastid 3
mitochondrion 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG95142
KRG96114
KRH20011
KRH32634
KRH57945
KRH57981
KRH66819
KRH67815

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G56350.1	KRG96114	AT2G36580.1	21798944
AT5G56350.1	KRH20011	AT2G36580.1	21798944
AT5G56350.1	KRH32634	AT2G36580.1	21798944
AT5G56350.1	KRH57945	AT2G36580.1	21798944
AT5G56350.1	KRH57981	AT2G36580.1	21798944
AT5G56350.1	KRH67815	AT2G36580.1	21798944
AT5G56350.1	KRG95142	AT4G33030.1	21798944
AT5G56350.1	KRH66819	AT4G33030.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH50504	Soybean	mitochondrion	97.81	98.0
VIT_06s0004g00130.t01	Wine grape	cytosol	90.04	89.33
AT5G56350.1	Thale cress	cytosol, mitochondrion, plastid	87.45	88.15
Solyc01g049650.2.1	Tomato	cytosol, mitochondrion, plastid	88.05	88.05
PGSC0003DMT400028308	Potato	plastid	88.05	88.05
CDY11933	Canola	cytosol, mitochondrion, plastid	75.5	87.33
Bra028934.1-P	Field mustard	cytosol, mitochondrion, plastid	81.67	87.23
Bra002822.1-P	Field mustard	cytosol	86.45	87.15
CDY32790	Canola	cytosol	72.51	86.46
CDY46578	Canola	cytosol	72.51	86.46
AT4G26390.1	Thale cress	mitochondrion	83.27	84.1
Bra009818.1-P	Field mustard	mitochondrion	38.25	83.84
CDY36795	Canola	endoplasmic reticulum	64.54	82.23
Zm00001d040446_P001	Maize	extracellular, plasma membrane	83.07	81.93
EES02767	Sorghum	cytosol	83.07	81.93
Os01t0276700-02	Rice	cytosol	82.07	80.78
KRH70138	Soybean	cytosol	15.34	79.38
TraesCS3D01G192800.1	Wheat	cytosol	80.88	79.14
TraesCS3A01G189300.1	Wheat	cytosol	80.88	79.14
HORVU3Hr1G039230.1	Barley	cytosol, plastid	80.68	78.95
KRG93166	Soybean	cytosol	78.69	77.45
TraesCS3B01G218500.1	Wheat	mitochondrion	80.48	77.25
KRH56500	Soybean	nucleus	78.29	76.91
TraesCS4D01G176600.1	Wheat	cytosol	77.89	76.82
TraesCS4B01G174700.1	Wheat	cytosol	77.89	76.82
TraesCS4A01G129900.1	Wheat	cytosol	77.49	76.42
KRH08482	Soybean	cytosol	77.89	76.07
CDY45309	Canola	plastid	46.81	75.56
EER92334	Sorghum	cytosol	77.29	74.9
HORVU3Hr1G039200.3	Barley	cytosol	66.53	73.89
KRH70137	Soybean	cytosol, endoplasmic reticulum	59.56	72.75
Os03t0325000-00	Rice	cytosol	61.16	71.06
KRG92070	Soybean	endoplasmic reticulum, nucleus	43.43	41.44
KRH34711	Soybean	endoplasmic reticulum	43.03	41.06
KRH67815	Soybean	cytosol, endoplasmic reticulum	42.63	40.61
KRH32634	Soybean	endoplasmic reticulum	42.43	40.42
KRH20011	Soybean	nucleus	42.23	40.23
KRG96114	Soybean	cytosol	42.43	39.3
KRH71730	Soybean	endoplasmic reticulum	14.94	38.86
KRH71733	Soybean	cytosol	10.76	38.57
KRH44705	Soybean	cytosol	14.14	36.22
KRH57945	Soybean	cytosol, endoplasmic reticulum	12.95	33.85
KRH38304	Soybean	cytosol	23.11	33.53
KRH57981	Soybean	cytosol	20.12	33.33
KRH77227	Soybean	plastid	35.86	33.03
KRH28271	Soybean	cytosol	16.53	31.92
KRG92440	Soybean	cytosol, nucleus	28.29	31.28
KRH34357	Soybean	nucleus	28.88	30.53
KRG91615	Soybean	nucleus	34.86	30.43
KRH35201	Soybean	plastid	34.86	30.28
KRH18451	Soybean	cytosol	15.34	30.2
KRH08770	Soybean	nucleus, plastid	34.06	29.64
KRG91145	Soybean	plastid	29.48	25.43
KRH35631	Soybean	plastid	29.28	25.26
KRH38305	Soybean	plastid	10.96	23.91
KRH24949	Soybean	cytosol, peroxisome, plastid	22.31	20.86

Protein Annotations

KEGG:00010+2.7.1.40	KEGG:00230+2.7.1.40	KEGG:00620+2.7.1.40	MapMan:2.1.1.8	Gene3D:2.40.33.10	Gene3D:3.20.20.60
Gene3D:3.40.1380.20	UniProt:A0A0R4J631	EMBL:ACUP02012210	EnsemblPlantsGene:GLYMA_20G024800	GO:GO:0000287	GO:GO:0003674
GO:GO:0003824	GO:GO:0004743	GO:GO:0005488	GO:GO:0005975	GO:GO:0006091	GO:GO:0006096
GO:GO:0006139	GO:GO:0008150	GO:GO:0008152	GO:GO:0009056	GO:GO:0009058	GO:GO:0009987
GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0030955	InterPro:IPR015806	InterPro:IPR036918
EnsemblPlants:KRG89466	ProteinID:KRG89466	ProteinID:KRG89466.1	PFAM:PF00224	PFAM:PF02887	PRINTS:PR01050
ScanProsite:PS00110	PANTHER:PTHR11817	PANTHER:PTHR11817:SF5	MetaCyc:PWY-1042	MetaCyc:PWY-2221	MetaCyc:PWY-5484
MetaCyc:PWY-5723	MetaCyc:PWY-6142	MetaCyc:PWY-6886	MetaCyc:PWY-6901	MetaCyc:PWY-7003	MetaCyc:PWY-7218
MetaCyc:PWY-7383	InterPro:Pyr_Knase	InterPro:Pyrv/PenolPyrv_Kinase-like_dom	InterPro:Pyrv_Knase-like_insert_dom_sf	InterPro:Pyrv_Knase_AS	InterPro:Pyrv_Knase_C
InterPro:Pyrv_Knase_C_sf	InterPro:Pyrv_Knase_brl	InterPro:Pyrv_Knase_insert_dom_sf	SUPFAM:SSF50800	SUPFAM:SSF51621	SUPFAM:SSF52935
TIGRFAMs:TIGR01064	UniParc:UPI0001B8373F	:	:	:	:

Description

hypothetical protein

Coordinates

chr20:+:2678970..2682421

Molecular Weight (calculated)

54347.3 Da

IEP (calculated)

7.216

GRAVY (calculated)

-0.012

Length

502 amino acids

Sequence
(BLAST)

001: MATTRIEAEK KPKTKIVCTL GPASRSVPMI EKLLRAGMNV ARFNFSHGSH EYHQETLDNL RAAMENTGIL CAVMLDTKGP EIRTGFLKDG KPIQLIQGNE
101: ITISTDYDLK GDEKTICMSY KKLPEDVKPG MVILCADGTI SFTVLSCDKQ AGLVQCRCEN SATLGERKNV NLPGVIVDLP TLTDKDKEDI LAWGIPNKID
201: MIALSFVRKG SDLVEVRKVL GKHAKNIMLM SKVENQEGVA NFDEILANSD AFMVARGDLG MEIPIEKIFL AQKVMIYKCN IQGKPVVTAT QMLESMIKSP
301: RPTRAEATDV ANAVLDGTDC VMLSGETAAG AYPDLAVQTM AKICIEAEST LDYGDVFKRI MEHSPVPMSP LESLASSAVR TANSARAALI LVLTRGGSTA
401: KLVAKYRPGM PILSVVVPEL KTDSFDWACS DEAPARHSLI FRGLVPVLSA ASARASHAET TEEAIEFAMQ HAKSKGLCHN GDSVVALHRV GTASIIKILT
501: VK

Best Arabidopsis Sequence Match ( AT5G56350.1 )
(BLAST)

001: MAMIEQRPKT KIVCTLGPAS RSVPMVEKLL RAGMNVARFN FSHGSHEYHQ ETLDNLHQAM LNTGILCAVM LDTKGPEIRT GFLKDGKPIQ LKQGQEITIS
101: TDYDLKGDEN TICMSYKKLA VDVNPGMVIL CADGTISLLV LSCDKENGTV RCRCENSAML GERKNVNLPG VVVDLPTLTE KDKEDIMQWG VPNQIDMIAL
201: SFVRKGSDLV QVRKLLGKHA KNILLMSKVE NQEGVANFDD ILVNSDAFMI ARGDLGMEIP IEKIFLAQKV MIYKCNIQGK PVVTATQMLE SMIKSPRPTR
301: AEATDVANAV LDGTDCVMLS GETAAGAYPE LAVRTMAKIC VEAESTLDYG DVFKRIMLYS PVPMSPLESL ASSAVRTANS ARATLIMVLT RGGSTARLVA
401: KYRPGMPILS VVVPEIKTDF FDWSCSDESP ARHSLIFRGL IPVLYAGSAR ASHDESTEEA IEFATQYGKE KELCKTGDSV VALLRVGNAS VIKILTVK

Arabidopsis Description

Pyruvate kinase [Source:UniProtKB/TrEMBL;Acc:Q9FM97]
SUBAcon: [mitochondrion,plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.