CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 4
mitochondrion 3
cytosol 1

Predictors

GFP

MS/MS

Papers

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG93968
KRG94095
KRG94880
KRG95629
KRG97587
KRG97672
KRG99257
KRH03685
KRH05863
KRH07089
KRH07729
KRH15146
KRH20252
KRH31200
KRH31287
KRH32110
KRH38103
KRH40007
KRH42233
KRH43030
KRH45741
KRH51797
KRH58237
KRH58350
KRH59137
KRH61121
KRH67309
KRH69484
KRH71326
KRH71854
KRH74672

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G22960.1	KRH05863	AT1G19680.1	21798944
AT3G22960.1	KRH15146	AT1G19680.1	21798944
AT3G22960.1	KRH51797	AT1G19680.1	21798944
AT3G22960.1	KRH61121	AT1G19680.1	21798944
AT3G22960.1	KRG99257	AT1G60010.1	21798944
AT3G22960.1	KRH45741	AT1G60010.1	21798944
AT3G22960.1	KRH69484	AT1G60010.1	21798944
AT3G22960.1	KRH74672	AT1G60010.1	21798944
AT3G22960.1	KRH07729	AT1G61590.1	21798944
AT3G22960.1	KRG95629	AT2G30580.1	21798944
AT3G22960.1	KRH32110	AT2G30580.1	21798944
AT3G22960.1	KRH67309	AT2G30580.1	21798944
AT3G22960.1	KRH71326	AT2G30580.1	21798944
AT3G22960.1	KRG93968	AT3G01690.1	21798944
AT3G22960.1	KRH07089	AT3G01690.1	21798944
AT3G22960.1	KRH58237	AT3G01690.1	21798944
AT3G22960.1	KRG97672	AT3G56130.1	21798944
AT3G22960.1	KRH31200	AT3G56130.1	21798944
AT3G22960.1	KRH43030	AT3G59920.1	21798944
AT3G22960.1	KRH59137	AT3G59920.1	21798944
AT3G22960.1	KRH42233	AT3G60360.1	21798944
AT3G22960.1	KRH58350	AT3G60360.1	21798944
AT3G22960.1	KRH38103	AT5G04600.1	21798944
AT3G22960.1	KRH40007	AT5G04600.1	21798944
AT3G22960.1	KRH71854	AT5G04600.1	21798944
AT3G22960.1	KRG97587	AT5G10210.1	21798944
AT3G22960.1	KRH31287	AT5G10210.1	21798944
AT3G22960.1	KRG93968	AT5G14390.1	21798944
AT3G22960.1	KRH07089	AT5G14390.1	21798944
AT3G22960.1	KRH58237	AT5G14390.1	21798944
AT3G22960.1	KRG94095	AT5G14660.1	21798944
AT3G22960.1	KRG94880	AT5G14660.1	21798944
AT3G22960.1	KRH03685	AT5G35410.1	21798944
AT3G22960.1	KRH20252	AT5G35410.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG92440	Soybean	cytosol, nucleus	93.68	98.02
GSMUA_Achr7P16760_001	Banana	cytosol	29.89	89.31
GSMUA_Achr2P17520_001	Banana	cytosol	77.26	87.8
GSMUA_Achr4P21110_001	Banana	cytosol	76.42	86.84
GSMUA_Achr4P28510_001	Banana	cytosol	76.42	86.84
VIT_05s0020g04480.t01	Wine grape	plastid	88.42	71.67
Os07t0181000-01	Rice	plastid	84.42	71.48
Bra001896.1-P	Field mustard	plastid	84.84	71.33
CDX92969	Canola	plastid	84.0	69.39
CDY40829	Canola	plastid	83.79	68.98
Bra023780.1-P	Field mustard	plastid	83.58	68.8
CDY35484	Canola	plastid	83.58	68.8
EER95951	Sorghum	plastid	84.42	68.78
Zm00001d034443_P001	Maize	plastid	82.74	68.47
TraesCS2D01G237900.1	Wheat	plastid	82.11	68.42
CDX94878	Canola	plastid	83.58	68.33
TraesCS2A01G240200.1	Wheat	plastid	81.68	68.07
KXG37365	Sorghum	plastid	82.32	67.65
Solyc09g082970.2.1	Tomato	plastid	84.21	67.45
AT3G22960.1	Thale cress	plastid	84.63	67.45
Zm00001d018961_P002	Maize	plastid	84.42	64.78
HORVU2Hr1G050140.1	Barley	plastid	81.26	63.91
TraesCS2B01G257800.1	Wheat	plastid	80.0	61.59
KRG91145	Soybean	plastid	57.89	47.25
KRH35631	Soybean	plastid	57.89	47.25
KRH38304	Soybean	cytosol	33.26	45.66
KRH44705	Soybean	cytosol	17.26	41.84
KRH18451	Soybean	cytosol	20.84	38.82
KRH70138	Soybean	cytosol	7.79	38.14
KRH77227	Soybean	plastid	41.68	36.33
KRG91615	Soybean	nucleus	43.37	35.83
KRH28271	Soybean	cytosol	19.58	35.77
KRH35201	Soybean	plastid	43.37	35.64
KRH08770	Soybean	nucleus, plastid	42.95	35.36
KRG89466	Soybean	endoplasmic reticulum, nucleus	30.53	28.88
KRH50504	Soybean	mitochondrion	30.11	28.54
KRG93166	Soybean	cytosol	30.11	28.04
KRH56500	Soybean	nucleus	30.11	27.98
KRH08482	Soybean	cytosol	29.05	26.85
KRH70137	Soybean	cytosol, endoplasmic reticulum	22.11	25.55
KRH24949	Soybean	cytosol, peroxisome, plastid	26.74	23.65
KRH34711	Soybean	endoplasmic reticulum	25.26	22.81
KRG92070	Soybean	endoplasmic reticulum, nucleus	25.05	22.62
KRH20011	Soybean	nucleus	24.42	22.01
KRH67815	Soybean	cytosol, endoplasmic reticulum	24.42	22.01
KRH32634	Soybean	endoplasmic reticulum	24.0	21.63
KRH71730	Soybean	endoplasmic reticulum	8.63	21.24
KRG96114	Soybean	cytosol	24.21	21.22
KRH38305	Soybean	plastid	9.89	20.43
KRH57981	Soybean	cytosol	12.21	19.14
KRH57945	Soybean	cytosol, endoplasmic reticulum	7.58	18.75
KRH71733	Soybean	cytosol	4.42	15.0

Protein Annotations

KEGG:00010+2.7.1.40	KEGG:00230+2.7.1.40	KEGG:00620+2.7.1.40	Gene3D:2.40.33.10	MapMan:3.12.8	Gene3D:3.20.20.60
Gene3D:3.40.1380.20	MapMan:5.1.2.1	EMBL:ACUP02006591	EnsemblPlantsGene:GLYMA_10G178900	GO:GO:0000287	GO:GO:0003674
GO:GO:0003824	GO:GO:0004743	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0005975	GO:GO:0006091	GO:GO:0006096	GO:GO:0006139	GO:GO:0008150
GO:GO:0008152	GO:GO:0009056	GO:GO:0009058	GO:GO:0009536	GO:GO:0009570	GO:GO:0009987
GO:GO:0016301	GO:GO:0016310	GO:GO:0016740	GO:GO:0030955	InterPro:IPR015806	InterPro:IPR036918
UniProt:K7LK37	EnsemblPlants:KRH34357	ProteinID:KRH34357	ProteinID:KRH34357.1	PFAM:PF00224	PFAM:PF02887
PRINTS:PR01050	ScanProsite:PS00110	PANTHER:PTHR11817	PANTHER:PTHR11817:SF14	MetaCyc:PWY-1042	MetaCyc:PWY-2221
MetaCyc:PWY-5484	MetaCyc:PWY-5723	MetaCyc:PWY-6142	MetaCyc:PWY-6886	MetaCyc:PWY-6901	MetaCyc:PWY-7003
MetaCyc:PWY-7218	MetaCyc:PWY-7383	InterPro:Pyr_Knase	InterPro:Pyrv/PenolPyrv_Kinase-like_dom	InterPro:Pyrv_Knase-like_insert_dom_sf	InterPro:Pyrv_Knase_AS
InterPro:Pyrv_Knase_C	InterPro:Pyrv_Knase_C_sf	InterPro:Pyrv_Knase_brl	InterPro:Pyrv_Knase_insert_dom_sf	SUPFAM:SSF50800	SUPFAM:SSF51621
SUPFAM:SSF52935	TIGRFAMs:TIGR01064	UniParc:UPI0002955D8C	:	:	:

Description

hypothetical protein

Coordinates

chr10:+:41199108..41205054

Molecular Weight (calculated)

52557.4 Da

IEP (calculated)

5.545

GRAVY (calculated)

-0.057

Length

475 amino acids

Sequence
(BLAST)

001: MHCGPLPRAA SSKLEALAVG GMNVARINMC HGTREWHKEV IDRVRGLNHE KGFAVAIMMD TEGSEIHMGD LGGASSAKAD DGEIWTFSVR AFDSALPQHT
101: INVNYEGFAE DVKVGDELLV DGGMVRFEVI QKIGPDVKCR CTDPGLLLPR ANLTFWRNGS LVRERNAMLP TISSKDWLDI DFGISEGVDF IAISFVKSAE
201: VITHLKSYIA ARSRDSDISV IAKIESIDSL KNIEEIILAA DGAMVARGDL GAQIPLEQVP SAQQRIVEIC RQLNKPVIVA SQLLESMIEY PTPTRAEVAD
301: VSEAVRQRAD ALMLSGESAM GQYPEKALTV LRSVSLRIEK WWREEKRYEA MLLPSVGSYF SEKISEEICN SAAKMANNLE VDALFVYTKT GHMASLLSRC
401: RPDCPIFAFT TTSSVRRRLN LQWGLIPFRL SFTDDMESNL NRTFSLLKAR NLIKSGDLVV AVSDMLQSIQ VMNVP

Best Arabidopsis Sequence Match ( AT3G22960.1 )
(BLAST)

001: MSQSIQFSTP SHTPHLLHLP HSQFNRPLSS ISFRRFPLTT IKYTSIRASS SSSPSPDLDS SSSSSSSQVL LSPNGTGAVK SDERSVVATA VTTDTSGIEV
101: DTVTEAELKE NGFRSTRRTK LICTIGPATC GFEQLEALAV GGMNVARLNM CHGTRDWHRG VIRSVRRLNE EKGFAVAIMM DTEGSEIHMG DLGGEASAKA
201: EDGEVWTFTV RAFDSSRPER TISVSYDGFA EDVRVGDELL VDGGMVRFEV IEKIGPDVKC LCTDPGLLLP RANLTFWRDG SLVRERNAML PTISSKDWLD
301: IDFGIAEGVD FIAVSFVKSA EVINHLKSYL AARSRGGEIG VIAKIESIDS LTNLEEIILA SDGAMVARGD LGAQIPLEQV PAAQQRIVQV CRALNKPVIV
401: ASQLLESMIE YPTPTRAEVA DVSEAVRQRS DALMLSGESA MGQFPDKALT VLRTVSLRIE RWWREEKRHE SVPLQAIGSS FSDKISEEIC NSAAKMANNL
501: GVDAVFVYTT SGHMASLVSR CRPDCPIFAF TTTTSVRRRL NLQWGLIPFR LSFSDDMESN LNKTFSLLKS RGMIKSGDLV IAVSDMLQSI QVMNVP

Arabidopsis Description

PKP1Plastidial pyruvate kinase 1, chloroplastic [Source:UniProtKB/Swiss-Prot;Acc:Q9LIK0]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.