CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

cytosol 6
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
plastid 1
nucleus 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, nucleus, plastid, secretory
BaCelLo:	cytosol
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	cytosol
Plant-mPloc:	cytosol, nucleus, plastid
PProwler:	mitochondrion
WoLF PSORT:	cytosol
YLoc:	cytosol

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH42483
KRH58580
KRH69305

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G59920.1	KRH69305	AT1G14340.1	21798944
AT3G59920.1	KRH42483	AT2G01950.1	19000166
AT3G59920.1	KRH58580	AT2G01950.1	19000166

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH43030	Soybean	cytosol, endoplasmic reticulum, nucleus	99.32	99.32
Solyc12g017570.1.1	Tomato	plastid	91.67	91.67
VIT_05s0094g01550.t01	Wine grape	cytosol	90.32	90.32
Solyc08g015860.2.1	Tomato	extracellular	90.09	90.09
PGSC0003DMT400035649	Potato	cytosol	90.09	90.09
GSMUA_Achr7P23620_001	Banana	cytosol	89.41	89.82
KRG99022	Soybean	cytosol	23.65	89.74
Os05t0304400-01	Rice	plasma membrane	88.29	88.09
Os05t0418000-01	Rice	cytosol, extracellular, plasma membrane, plastid	88.06	87.87
HORVU1Hr1G064490.8	Barley	cytosol	88.06	87.47
EES18242	Sorghum	cytosol	87.61	87.42
KXG22017	Sorghum	cytosol	87.61	87.42
TraesCS1B01G271700.1	Wheat	cytosol	87.39	87.19
TraesCS1D01G260900.1	Wheat	cytosol, golgi	87.39	87.19
GSMUA_Achr6P18380_001	Banana	cytosol	87.61	87.02
TraesCS1A01G260900.1	Wheat	cytosol	86.94	86.74
CDY17885	Canola	cytosol	86.49	86.49
CDX79782	Canola	cytosol	86.26	86.26
Bra004805.1-P	Field mustard	cytosol	86.26	86.26
TraesCS1A01G261000.1	Wheat	cytosol	86.49	86.1
CDX67817	Canola	cytosol	85.81	85.81
CDX98399	Canola	cytosol	85.81	85.81
TraesCS1B01G271800.2	Wheat	cytosol, golgi	86.04	85.65
Bra003395.1-P	Field mustard	cytosol	85.59	85.59
CDX89038	Canola	cytosol	85.59	85.59
Bra014523.1-P	Field mustard	cytosol	85.59	85.59
AT3G59920.1	Thale cress	cytosol	85.59	85.59
CDY45775	Canola	cytosol	85.36	85.36
Zm00001d010255_P001	Maize	cytosol, extracellular	87.16	85.24
AT2G44100.1	Thale cress	cytosol	85.36	85.17
Bra007493.1-P	Field mustard	cytosol	84.23	84.04
CDY05962	Canola	cytosol	83.56	83.56
Zm00001d010251_P003	Maize	cytosol	86.94	82.48
EER98346	Sorghum	cytosol	82.66	82.29
TraesCS5A01G041100.1	Wheat	cytosol	82.43	82.25
TraesCS5B01G042400.1	Wheat	cytosol	82.43	82.25
CDX71860	Canola	cytosol	75.68	82.15
Os07t0271000-01	Rice	plasma membrane	82.21	81.84
TraesCS5D01G048100.1	Wheat	cytosol	81.98	81.8
KRH49823	Soybean	cytosol, endoplasmic reticulum	81.31	81.12
GSMUA_Achr10P...	Banana	cytosol	80.86	80.86
KRH42088	Soybean	cytosol	80.63	80.45
Zm00001d007315_P001	Maize	cytosol	31.98	79.78
HORVU1Hr1G064480.1	Barley	cytosol	79.5	79.15
Zm00001d038200_P002	Maize	plastid	86.04	75.2
Zm00001d005498_P001	Maize	cytosol	82.88	74.49
TraesCS1D01G261000.1	Wheat	golgi	86.26	73.37
KRH71651	Soybean	cytosol, nucleus, peroxisome	31.31	70.92
HORVU5Hr1G009840.1	Barley	cytosol	82.66	67.22
Zm00001d009786_P001	Maize	plastid	46.4	60.06
Zm00001d011867_P001	Maize	cytosol	47.97	58.2
Zm00001d016318_P002	Maize	cytosol	33.56	52.65
HORVU2Hr1G104740.2	Barley	mitochondrion	10.59	50.0
HORVU7Hr1G075170.1	Barley	mitochondrion	12.16	49.09
KRH66563	Soybean	cytosol	48.42	38.6
KRH05675	Soybean	extracellular, vacuole	9.46	21.11
KRH28917	Soybean	cytosol, peroxisome, plastid	23.65	19.06
KRH76543	Soybean	cytosol, peroxisome, plasma membrane	23.65	18.99

Protein Annotations

Gene3D:1.10.405.10	MapMan:22.8.4.2	Gene3D:3.30.519.10	Gene3D:3.50.50.60	EMBL:ACUP02003303	InterPro:FAD/NAD-bd_sf
InterPro:GDP_dissociation_inhibitor	EnsemblPlantsGene:GLYMA_05G167800	GO:GO:0003674	GO:GO:0005092	GO:GO:0005093	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0006810	GO:GO:0007154	GO:GO:0007165	GO:GO:0007264
GO:GO:0008150	GO:GO:0009987	GO:GO:0015031	GO:GO:0030234	GO:GO:0050790	UniProt:I1K489
InterPro:IPR036188	EnsemblPlants:KRH59137	ProteinID:KRH59137	ProteinID:KRH59137.1	PFAM:PF00996	PRINTS:PR00891
PRINTS:PR00892	PANTHER:PTHR11787	PANTHER:PTHR11787:SF10	InterPro:RabGDI	SUPFAM:SSF51905	SUPFAM:SSF54373
UniParc:UPI0002339B41	SEG:seg	:	:	:	:

Description

hypothetical protein

Coordinates

chr5:-:35840858..35846833

Molecular Weight (calculated)

49664.4 Da

IEP (calculated)

5.344

GRAVY (calculated)

-0.253

Length

444 amino acids

Sequence
(BLAST)

001: MDEEYDVIVL GTGLKECILS GLLSVDGLKV LHMDRNDYYG GESTSLNLIQ LWKRFRGDDK PPPHLGASRD YNIDMNPKFI MANGNLVRVL IHTDVTKYLY
101: FKAVDGSYVF NKGKVHKVPA NDMEALKSPL MGLFEKRRAR KFFIYVQNYN ESDPKTHEGM DLTRVTTKEL IAKYGLDDNT IDFIGHALAL HSDDRYLAEP
201: ALDTVKRMKL YAESLARFQG GSPYIYPLYG LGELPQAFAR LSAVYGGTYM LNKPECKVEF DEEGKVVGVT SEGETAKCKK VVCDPSYLPG KVRKVGRVAR
301: AIAIMSHPIP STDDSHSVQI ILPQKQLGRK SDMYLFCCSY THNVAPKGKF IAFVSTEAET DQPAIELKPG IDLLGPVDEI FFDIYDRYEP VNEPTLDNCF
401: ISTSYDATTH FESTVDDVLN MYTLITGKVI DLSVDLSAAS AAEE

Best Arabidopsis Sequence Match ( AT3G59920.1 )
(BLAST)

001: MDEEYEVIVL GTGLKECILS GLLSVDGVKV LHMDRNDYYG GESTSLNLNQ LWKKFRGEEK APEHLGASRD YNVDMMPKFM MGNGKLVRTL IHTDVTKYLS
101: FKAVDGSYVF VKGKVQKVPA TPMEALKSSL MGIFEKRRAG KFFSFVQEYD EKDPKTHDGM DLTRVTTKEL IAKYGLDGNT IDFIGHAVAL HTNDQHLDQP
201: AFDTVMRMKL YAESLARFQG TSPYIYPLYG LGELPQAFAR LSAVYGGTYM LNKPECKVEF DEGGKVIGVT SEGETAKCKK IVCDPSYLPN KVRKIGRVAR
301: AIAIMSHPIP NTNDSHSVQV IIPQKQLARK SDMYVFCCSY SHNVAPKGKF IAFVSTDAET DNPQTELKPG TDLLGPVDEI FFDMYDRYEP VNEPELDNCF
401: ISTSYDATTH FETTVADVLN MYTLITGKQL DLSVDLSAAS AAEE

Arabidopsis Description

GDI2Guanosine nucleotide diphosphate dissociation inhibitor [Source:UniProtKB/TrEMBL;Acc:A0A178VFJ4]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.