CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 5
endoplasmic reticulum 5
vacuole 5
plasma membrane 8
golgi 5
cytosol 2
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	cytosol, plastid, secretory
BaCelLo:	secretory
EpiLoc:	cytosol
iPSORT:	secretory
MultiLoc:	plasma membrane
Plant-mPloc:	cytosol, plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
KRG89812
KRG94975
KRH18664
KRH31638
KRH69070

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G48010.1	KRG89812	AT2G41090.1	17360592
AT2G48010.1	KRG94975	AT2G41090.1	17360592
AT2G48010.1	KRH18664	AT2G41090.1	17360592
AT2G48010.1	KRH31638	AT4G14640.1	17360592
AT2G48010.1	KRH69070	AT4G14640.1	17360592
AT2G48010.1	KRG89812	AT5G21274.1	17360592
AT2G48010.1	KRG94975	AT5G21274.1	17360592
AT2G48010.1	KRH18664	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG95455	Soybean	plasma membrane	83.13	84.43
KRH67133	Soybean	cytosol	38.7	82.24
KRG95456	Soybean	plasma membrane	60.68	68.06
PGSC0003DMT400095174	Potato	cytosol	27.09	66.54
KRH71493	Soybean	plasma membrane	62.07	64.16
KRH31961	Soybean	plasma membrane	62.38	64.07
AT2G48010.1	Thale cress	plasma membrane	55.73	58.35
Solyc09g074240.1.1	Tomato	plasma membrane	57.59	58.31
Bra023063.1-P	Field mustard	plasma membrane	55.57	57.62
CDX84563	Canola	plasma membrane	55.57	57.62
CDX79672	Canola	plasma membrane	54.8	57.1
GSMUA_Achr8P33610_001	Banana	plastid	30.03	56.56
VIT_07s0104g00840.t01	Wine grape	plasma membrane	56.35	55.57
TraesCS4B01G109400.1	Wheat	plasma membrane	45.36	48.11
Os01t0384300-00	Rice	plasma membrane	46.75	47.19
TraesCS4A01G205800.1	Wheat	plasma membrane	46.13	47.15
TraesCS4D01G106700.1	Wheat	plasma membrane	45.98	46.99
KXG29404	Sorghum	plasma membrane	45.98	46.55
HORVU4Hr1G019460.1	Barley	plasma membrane	45.36	46.51
Zm00001d015163_P001	Maize	plasma membrane	32.51	39.11
KRH17993	Soybean	plasma membrane	31.73	35.34
KRH17996	Soybean	plasma membrane	33.13	34.91
KRH59164	Soybean	plasma membrane	25.85	34.72
KRH10412	Soybean	plasma membrane	34.67	34.51
KRH44057	Soybean	plasma membrane	34.37	33.59
KRH17995	Soybean	plasma membrane	31.89	33.39
KRH43048	Soybean	plasma membrane	31.11	30.41

Protein Annotations

Gene3D:1.10.510.10	EntrezGene:100786298	MapMan:18.4.1.23	Gene3D:3.30.200.20	EMBL:ACUP02002037	EnsemblPlantsGene:GLYMA_03G149000
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488
GO:GO:0005524	GO:GO:0005575	GO:GO:0005623	GO:GO:0005886	GO:GO:0006464	GO:GO:0006468
GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016020	GO:GO:0016021	GO:GO:0016301
GO:GO:0016740	GO:GO:0019538	UniProt:I1JNQ1	InterPro:IPR000719	EnsemblPlants:KRH67131	ProteinID:KRH67131
ProteinID:KRH67131.1	InterPro:Kinase-like_dom_sf	PFAM:PF00069	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011
PANTHER:PTHR27006	PANTHER:PTHR27006:SF36	InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112
InterPro:Ser/Thr_kinase_AS	SignalP:SignalP-TM	TMHMM:TMhelix	UniParc:UPI00023C1C4D	SEG:seg	:

Description

hypothetical protein

Coordinates

chr3:+:36453913..36456220

Molecular Weight (calculated)

71074.8 Da

IEP (calculated)

6.719

GRAVY (calculated)

-0.093

Length

646 amino acids

Sequence
(BLAST)

001: MLSMSLPFYY FYIFYVVFLL TLNIFPPASL AANNTLPCPL NITVLRVVSG GRRPSFDSGT KCHYILQALH LLQADYLRRS SLFVPPLNTS ESCWNTFQSF
101: INEADPSISD IRSSCGFRTE WISQGCMNIT TKQQFEDIVP RNAIQPVQDD CNQPLENNAP CALCTTKLSS MLSYLTGTTQ GNVTDCRAYT QIYAASLSDQ
201: YGVTDPGTSK CLFGLDFSSS GSGGKRRRTI VIAVVSVFCV VSLSVFASLW AYLRFKKRLE VEKRKGAGIS ELGLGSGLDS INQSTTLIRF SFDEIKKATR
301: NFSRDNIIGS GGYGNVYKGM LLDGSQVAFK RFKNCSVAGD ASFTHEVEVI ASVRHVNLVT LRGYCTATTN LEGHQRIIVT DLMENGSLYD HLFGSAKKNL
401: TWPIRQKIAL GTARGLAYLH YGAQPSIIHR DIKASNILLD HNFEAKVADF GLAKFNPEGM THMSTRVAGT MGYVAPEYAL YGQLTERSDV FSFGVVLLEL
501: LSGRKALQTD DDGQPAALTD FAWSLVRNGS ALDVVEDGIP EPGPPEVLEK YVLVAVLCSH PQLYARPTMD QVVKMLETDE SVPSLMERPI PFIAGRLDIE
601: KSALSNSGQL CSPTGYQAYT LQARRKSNTK EEEEEGSSVA ETVTTD

Best Arabidopsis Sequence Match ( AT2G48010.1 )
(BLAST)

001: MLFLRRIAVV FFVFTSFSAA QNSTCPLDFS VLEPFRRPKP DGATTCQYLL QGLRLLYSHH LRQTGSFLPP PESAASCWAA LQSSVAGFLP RFDVRSTCGF
101: QTPWISQGCM DITTRSQFES LIPNSSLATT AMRCNTSLES NTPCASCTQS LSAFQPYLSG PSLGNVSDCA SFPSIYAAAF ANSLGPTDKG TAKCLFQLDL
201: ASPTSSGANK VKVLVSSFSV LLVASVLVIT AWFWYCRRKK SKLLKPRDTS LEAGTQSRLD SMSESTTLVK FSFDEIKKAT NNFSRHNIIG RGGYGNVFKG
301: ALPDGTQVAF KRFKNCSAGG DANFAHEVEV IASIRHVNLL ALRGYCTATT PYEGHQRIIV CDLVSNGSLH DHLFGDLEAQ LAWPLRQRIA LGMARGLAYL
401: HYGAQPSIIH RDIKASNILL DERFEAKVAD FGLAKFNPEG MTHMSTRVAG TMGYVAPEYA LYGQLTEKSD VYSFGVVLLE LLSRRKAIVT DEEGQPVSVA
501: DWAWSLVREG QTLDVVEDGM PEKGPPEVLE KYVLIAVLCS HPQLHARPTM DQVVKMLESN EFTVIAIPQR PIPLVACREE IDRSVSSSSG SGKLTSPTGY
601: QAFSFGGDGP SGNTNTT

Arabidopsis Description

RKF3Probable LRR receptor-like serine/threonine-protein kinase RKF3 [Source:UniProtKB/Swiss-Prot;Acc:P93050]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.