CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane

Predictor Summary:

extracellular 4
endoplasmic reticulum 4
vacuole 4
plasma membrane 7
golgi 4
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	plasma membrane
Any Predictor:	mitochondrion, plastid, secretory
BaCelLo:	secretory
ChloroP:	plastid
iPSORT:	secretory
MultiLoc:	plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	mitochondrion
WoLF PSORT:	plasma membrane
YLoc:	plasma membrane

PPI

Inferred distinct locusB in Crop

locusB	locations
EES01631
EES09201
EES16322
KXG37587
KXG37588
OQU78227

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G48010.1	EES01631	AT2G41090.1	17360592
AT2G48010.1	EES09201	AT2G41090.1	17360592
AT2G48010.1	EES16322	AT2G41090.1	17360592
AT2G48010.1	OQU78227	AT2G41090.1	17360592
AT2G48010.1	KXG37587	AT4G14640.1	17360592
AT2G48010.1	KXG37588	AT4G14640.1	17360592
AT2G48010.1	EES01631	AT5G21274.1	17360592
AT2G48010.1	EES09201	AT5G21274.1	17360592
AT2G48010.1	EES16322	AT5G21274.1	17360592
AT2G48010.1	OQU78227	AT5G21274.1	17360592

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Zm00001d015163_P001	Maize	plasma membrane	78.53	93.3
Os01t0384300-00	Rice	plasma membrane	78.53	78.28
TraesCS4A01G205800.1	Wheat	plasma membrane	76.33	77.06
TraesCS4D01G106700.1	Wheat	plasma membrane	76.02	76.74
HORVU4Hr1G019460.1	Barley	plasma membrane	75.71	76.67
TraesCS4B01G109400.1	Wheat	plasma membrane	72.57	76.03
KRH67133	Soybean	cytosol	31.82	66.78
GSMUA_Achr8P33610_001	Banana	plastid	34.33	63.85
PGSC0003DMT400095174	Potato	cytosol	25.39	61.6
AT2G48010.1	Thale cress	plasma membrane	49.06	50.73
CDX79672	Canola	plasma membrane	49.06	50.48
CDX84563	Canola	plasma membrane	49.22	50.4
Bra023063.1-P	Field mustard	plasma membrane	49.22	50.4
KRH71493	Soybean	plasma membrane	49.06	50.08
KRH31961	Soybean	plasma membrane	48.59	49.28
KRG95456	Soybean	plasma membrane	44.36	49.13
VIT_07s0104g00840.t01	Wine grape	plasma membrane	50.16	48.85
Solyc09g074240.1.1	Tomato	plasma membrane	48.59	48.59
VIT_14s0030g00350.t01	Wine grape	cytosol	41.54	48.45
KRG95455	Soybean	plasma membrane	46.4	46.54
KRH67131	Soybean	plasma membrane	46.55	45.98
OQU78028	Sorghum	plasma membrane	35.42	34.5
EES03987	Sorghum	mitochondrion	34.48	31.98

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.1.23	Gene3D:3.30.200.20	UniProt:A0A194YMG1	GO:GO:0000166	GO:GO:0003674
GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575
GO:GO:0005623	GO:GO:0005886	GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152
GO:GO:0009987	GO:GO:0016020	GO:GO:0016021	GO:GO:0016301	GO:GO:0016310	GO:GO:0016740
GO:GO:0019538	InterPro:IPR000719	EnsemblPlants:KXG29404	ProteinID:KXG29404	ProteinID:KXG29404.2	InterPro:Kinase-like_dom_sf
PFAM:PF00069	ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR27006	PANTHER:PTHR27006:SF36
InterPro:Prot_kinase_dom	InterPro:Protein_kinase_ATP_BS	SMART:SM00220	EnsemblPlantsGene:SORBI_3004G031400	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS
SignalP:SignalP-noTM	TMHMM:TMhelix	UniParc:UPI000B8BA742	SEG:seg	:	:

Description

hypothetical protein

Coordinates

chr4:+:2531310..2537576

Molecular Weight (calculated)

67213.8 Da

IEP (calculated)

7.290

GRAVY (calculated)

0.211

Length

638 amino acids

Sequence
(BLAST)

001: MCRPHLLPLL LFALLLLLRP TPLASQPAAP SPAPQCALNF TALLPFLAPP LPSDDATRCA VATQSVAFLL SLHLAATSSF VLPPDASSCL SPLRATLPYP
101: LPASGCGLSG LDAVLASPGC GNVSTVADFD ALVPPSSRQD IDASCDRDLS AVPDCTACST ALSKAAAAYL LAVFPNATGN NNVTGCVQYP SIYAGAEASP
201: RGPADPATAY CLFLLKANPP QSHSSGTAPW VYGVAFGSLA AVLLLAAAVG CCFVVRRRQA RAAAAALAAD SRSKRSQAME SISASTTLVK FTYDDIKAAT
301: DGFARKSIIG HGGFGNVYKG VLRDGAEVAV KRFKNCSAAG DAAFAHEVEV VASVRHVNLV ALRGYCIATT QREGHQRMIV CDLMHNGSLY DHLFGAGECQ
401: LPWPVRQRIA IGMARGLSYL HRGAQPAIIH RDIKASNILL DDEFEAKVAD FGLAKFAPEG MTHVSTRVAG TLGYVAPEYA LYGQLTEKSD VYSFGVVLLE
501: LLSGKKAFIS LSEGQSFVLT DWAWSLVRRG RTAEVIQEGM VDPGPTKVME KYVLVGALCT HPQLHARPTM EQALKILEAD SVPSPLIIPE RPIPVVANLA
601: EIERSASSSG SGQLFSPSGF RSFIHANVDA AVVSPNET

Best Arabidopsis Sequence Match ( AT2G48010.1 )
(BLAST)

001: MLFLRRIAVV FFVFTSFSAA QNSTCPLDFS VLEPFRRPKP DGATTCQYLL QGLRLLYSHH LRQTGSFLPP PESAASCWAA LQSSVAGFLP RFDVRSTCGF
101: QTPWISQGCM DITTRSQFES LIPNSSLATT AMRCNTSLES NTPCASCTQS LSAFQPYLSG PSLGNVSDCA SFPSIYAAAF ANSLGPTDKG TAKCLFQLDL
201: ASPTSSGANK VKVLVSSFSV LLVASVLVIT AWFWYCRRKK SKLLKPRDTS LEAGTQSRLD SMSESTTLVK FSFDEIKKAT NNFSRHNIIG RGGYGNVFKG
301: ALPDGTQVAF KRFKNCSAGG DANFAHEVEV IASIRHVNLL ALRGYCTATT PYEGHQRIIV CDLVSNGSLH DHLFGDLEAQ LAWPLRQRIA LGMARGLAYL
401: HYGAQPSIIH RDIKASNILL DERFEAKVAD FGLAKFNPEG MTHMSTRVAG TMGYVAPEYA LYGQLTEKSD VYSFGVVLLE LLSRRKAIVT DEEGQPVSVA
501: DWAWSLVREG QTLDVVEDGM PEKGPPEVLE KYVLIAVLCS HPQLHARPTM DQVVKMLESN EFTVIAIPQR PIPLVACREE IDRSVSSSSG SGKLTSPTGY
601: QAFSFGGDGP SGNTNTT

Arabidopsis Description

RKF3Probable LRR receptor-like serine/threonine-protein kinase RKF3 [Source:UniProtKB/Swiss-Prot;Acc:P93050]
SUBAcon: [plasma membrane]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.