Factsheet for Os03t0223400-01

Subcellular Localization

min:

: max

Winner_takes_all: plasma membrane, cytosol

Predictor Summary:

nucleus 2
cytosol 4
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

plasma membrane: 27800704

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
OQU92979	Sorghum	cytosol	91.04	91.04
TraesCS4D01G240700.1	Wheat	cytosol	88.24	88.98
TraesCS4B01G240900.1	Wheat	cytosol, golgi	88.24	88.98
TraesCS4A01G063800.1	Wheat	cytosol	87.96	88.7
GSMUA_Achr3P20250_001	Banana	cytosol, plasma membrane	86.55	86.8
GSMUA_AchrUn_...	Banana	cytosol	86.55	86.8
GSMUA_Achr3P07420_001	Banana	cytosol, plasma membrane	85.71	85.96
KRH17323	Soybean	cytosol	85.43	85.67
KRH30929	Soybean	endoplasmic reticulum	85.43	85.67
Os02t0735200-02	Rice	cytosol	85.43	85.67
VIT_14s0006g00350.t01	Wine grape	cytosol	85.43	85.67
KRH72976	Soybean	cytosol	84.59	84.83
KRG97948	Soybean	cytosol, endoplasmic reticulum, nucleus	84.31	84.55
HORVU4Hr1G066860.5	Barley	plasma membrane	88.52	82.51
Zm00001d048050_P004	Maize	cytosol	76.47	79.13
Zm00001d028260_P005	Maize	cytosol	86.27	78.77
Os03t0712800-02	Rice	cytosol, plastid	80.67	77.84
KRH72975	Soybean	cytosol	52.94	74.7
Os04t0659100-01	Rice	plastid	77.59	64.72
KRH17322	Soybean	cytosol	45.38	64.29

Protein Annotations

KEGG:00220+6.3.1.2	KEGG:00250+6.3.1.2	KEGG:00630+6.3.1.2	KEGG:00910+6.3.1.2	MapMan:25.1.5.1.1	Gene3D:3.10.20.70
Gene3D:3.30.590.40	EntrezGene:4332108	ProteinID:AAN05339.1	EMBL:AB180688	ProteinID:ABF94717.1	EMBL:AK243037
ProteinID:BAF11338.1	ProteinID:BAS83037.1	ProteinID:EEE58620.1	GO:GO:0000166	GO:GO:0003674	GO:GO:0003824
GO:GO:0004356	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575	GO:GO:0005622	GO:GO:0005623
GO:GO:0005737	GO:GO:0006542	GO:GO:0006807	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058
GO:GO:0009987	GO:GO:0016874	InterPro:Gln_synt_N	InterPro:Gln_synt_b-grasp	InterPro:Gln_synth/guanido_kin_cat_dom	InterPro:Gln_synth_N_conserv_site
InterPro:Gln_synth_cat_dom	InterPro:Gln_synth_gly_rich_site	InterPro:IPR036651	EnsemblPlantsGene:Os03g0223400	EnsemblPlants:Os03t0223400-01	UniProt:P14654
PFAM:PF00120	PFAM:PF03951	ScanProsite:PS00180	ScanProsite:PS00181	PANTHER:PTHR20852	PANTHER:PTHR20852:SF78
SMART:SM01230	SUPFAM:SSF54368	SUPFAM:SSF55931	UniParc:UPI000012B69C	EMBL:X14244	RefSeq:XP_015631679.1

Description

GLUTAMINE SYNTHETASE 1;2Cytosolic glutamine synthetase, Ammonium assimilation (Os03t0223400-01)

Coordinates

chr3:-:6457915..6462146

Molecular Weight (calculated)

39260.4 Da

IEP (calculated)

5.937

GRAVY (calculated)

-0.384

Length

357 amino acids

Sequence
(BLAST)

001: MANLTDLVNL NLSDCSDKII AEYIWVGGSG IDLRSKARTV KGPITDVSQL PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGDNILV MCDCYTPQGE
101: PIPTNKRHSA AKIFSHPDVV AEVPWYGIEQ EYTLLQKDVN WPLGWPVGGF PGPQGPYYCA AGAEKAFGRD IVDAHYKACI YAGINISGIN GEVMPGQWEF
201: QVGPSVGIAA ADQVWVARYI LERVTEVAGV VLSLDPKPIP GDWNGAGAHT NFSTKSMREP GGYEVIKKAI DKLALRHKEH IAAYGEGNER RLTGRHETAD
301: INTFKWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTGMIAET TLLWKQN

Best Arabidopsis Sequence Match ( AT1G66200.1 )
(BLAST)

001: MSLLADLVNL DISDNSEKII AEYIWVGGSG MDMRSKARTL PGPVTDPSKL PKWNYDGSST GQAPGQDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE
101: PIPTNKRHAA AEIFANPDVI AEVPWYGIEQ EYTLLQKDVN WPLGWPIGGF PGPQGPYYCS IGADKSFGRD IVDAHYKASL YAGINISGIN GEVMPGQWEF
201: QVGPSVGISA ADEIWIARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT NYSTKSMREE GGYEIIKKAI EKLGLRHKEH ISAYGEGNER RLTGHHETAD
301: INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIAET TLLWNP

Arabidopsis Description

ATGSR2Glutamine synthetase [Source:UniProtKB/TrEMBL;Acc:F4ID91]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.