Factsheet for Os08t0459600-01

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

plastid 1
mitochondrion 3
peroxisome 3
cytosol 2

Predictors

GFP

MS/MS

Papers

peroxisome: 17938955

cytosol: 19685898
plasma membrane: 27800704

gfp PMID: 17938955 doi

T Tani, H Sobajima, K Okada, T Chujo, S Arimura, N Tsutsumi, M Nishimura, H Seto, H Nojiri, H Yamane

Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

msms PMID: 27800704 doi

J Cao, C Yang, L Li, L Jiang, Y Wu, C Wu, Q Bu, G Xia, X Liu, Y Luo, J Liu

Huai'an Institute of Agricultural Sciences, Huai'an, 223001, China., Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China; and., Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin, 150081, China., State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

msms PMID: 19685898 doi

KA Doroshenk, AJ Crofts, RT Morris, JJ Wyrick, TW Okita

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.

PPI

Inferred distinct locusB in Crop

locusB	locations
Os12t0288600-01
Os12t0288801-00

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G06050.1	Os12t0288600-01	AT1G13030.1	21798944
AT2G06050.1	Os12t0288801-00	AT1G13030.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
TraesCS7A01G412400.1	Wheat	mitochondrion, peroxisome	89.09	90.0
TraesCS7D01G405500.1	Wheat	golgi	89.09	90.0
TraesCS7B01G311600.1	Wheat	mitochondrion, peroxisome	89.09	90.0
Zm00001d032049_P002	Maize	extracellular, mitochondrion	87.31	86.22
Zm00001d050107_P001	Maize	extracellular, mitochondrion	87.06	85.96
EES13971	Sorghum	peroxisome, plastid	87.31	85.57
TraesCS5B01G487600.1	Wheat	mitochondrion	75.13	77.69
KRH02619	Soybean	nucleus	75.38	74.62
KRH19303	Soybean	nucleus	74.87	74.12
GSMUA_Achr7P02310_001	Banana	mitochondrion	63.96	73.47
HORVU7Hr1G095960.1	Barley	plastid	89.59	72.93
Solyc07g007870.2.1	Tomato	plastid	73.1	72.73
VIT_11s0016g01230.t01	Wine grape	plastid	73.1	72.36
AT2G06050.1	Thale cress	cytosol, plastid	71.32	71.87
CDY50415	Canola	peroxisome	70.81	71.72
CDX93099	Canola	peroxisome	70.81	71.72
PGSC0003DMT400079356	Potato	plastid	71.83	70.75
Bra013161.1-P	Field mustard	peroxisome	69.8	70.69
KRH02618	Soybean	mitochondrion	67.01	69.29
KRH19304	Soybean	mitochondrion, plastid	67.77	68.81
Os06t0216200-00	Rice	plasma membrane	52.54	55.05
Os06t0215600-02	Rice	plastid	51.52	54.28
Os06t0215900-02	Rice	cytosol	51.52	53.42
Os06t0216000-01	Rice	plastid	17.01	53.17
Os06t0215500-01	Rice	cytosol, nucleus, peroxisome	51.52	51.92
Os02t0559400-01	Rice	cytosol, peroxisome	48.98	47.54
Os04t0443750-00	Rice	cytoskeleton, cytosol, peroxisome	22.59	47.09
Os06t0216300-01	Rice	plastid	18.02	46.71
Os01t0369900-01	Rice	cytosol	16.24	35.36
Os01t0370000-01	Rice	mitochondrion	33.5	32.04

Protein Annotations

MapMan:11.7.1.6	Gene3D:3.20.20.70	EntrezGene:4345762	EMBL:AK071203	EMBL:AK104843	InterPro:Aldolase_TIM
ProteinID:BAD09599.1	ProteinID:BAD09954.1	ProteinID:BAF23889.1	ProteinID:BAT05755.1	ProteinID:EAZ42984.1	GO:GO:0000003
GO:GO:0000166	GO:GO:0003674	GO:GO:0003824	GO:GO:0005488	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0005777	GO:GO:0006629	GO:GO:0006631	GO:GO:0006633
GO:GO:0006950	GO:GO:0007275	GO:GO:0008150	GO:GO:0008152	GO:GO:0009058	GO:GO:0009605
GO:GO:0009607	GO:GO:0009620	GO:GO:0009695	GO:GO:0009791	GO:GO:0009908	GO:GO:0009987
GO:GO:0010181	GO:GO:0010193	GO:GO:0016491	GO:GO:0016629	GO:GO:0031408	GO:GO:0048443
GO:GO:0055114	InterPro:IPR013785	EnsemblPlantsGene:Os08g0459600	EnsemblPlants:Os08t0459600-01	InterPro:OxRdtase_FMN_N	PFAM:PF00724
PANTHER:PTHR22893	PANTHER:PTHR22893:SF67	UniProt:Q6Z965	SUPFAM:SSF51395	UniParc:UPI000023AE96	RefSeq:XP_015650809.1
SEG:seg	:	:	:	:	:

Description

12-OXOPHYTODIENOATE REDUCTASE 712-oxophytodienoate reductase (EC:1.3.1.42), Jasmonic acid (JA) biosynthesis (Os08t0459600-01)

Coordinates

chr8:+:22547184..22550675

Molecular Weight (calculated)

43680.0 Da

IEP (calculated)

8.730

GRAVY (calculated)

-0.408

Length

394 amino acids

Sequence
(BLAST)

001: MDRPPPDQQR QKQAPLFSPY QMPRFRLNHR VVLAPMTRCR AIGGVPGPAL AEYYAQRTTQ GGLLISEGTV VSPAGPGFPH VPGIYNQEQT DAWKKVVDAV
101: HAKGGIFFCQ LWHVGRASHQ VYQPNGAAPI SSTDKPISAR WRILMPDGSY GKYPKPRRLA ASEIPEIVEQ YRQAAINAIE AGFDGIEIHG AHGYIIDQFL
201: KDGINDRTDE YGGSLSNRCR FLLEVTRAVV SAIGADRVAV RISPAIDHLD AYDSDPIKLG MAVVERLNAL QQQSGRLAYL HVTQPRYTAY GQTESGQHGS
301: AEEESRLMRT LRGTYQGTFM CSGGYTRELG LEAVESGDAD LVSYGRLFIS NPDLVERFRL NAGLNKYVRK TFYTPDPVVG YTDYPFLGQP KSRM

Best Arabidopsis Sequence Match ( AT2G06050.3 )
(BLAST)

001: MTAAQGNSNE TLFSSYKMGR FDLSHRVVLA PMTRCRALNG VPNAALAEYY AQRTTPGGFL ISEGTMVSPG SAGFPHVPGI YSDEQVEAWK QVVEAVHAKG
101: GFIFCQLWHV GRASHAVYQP NGGSPISSTN KPISENRWRV LLPDGSHVKY PKPRALEASE IPRVVEDYCL SALNAIRAGF DGIEIHGAHG YLIDQFLKDG
201: INDRTDQYGG SIANRCRFLK QVVEGVVSAI GASKVGVRVS PAIDHLDATD SDPLSLGLAV VGMLNKLQGV NGSKLAYLHV TQPRYHAYGQ TESGRQGSDE
301: EEAKLMKSLR MAYNGTFMSS GGFNKELGMQ AVQQGDADLV SYGRLFIANP DLVSRFKIDG ELNKYNRKTF YTQDPVVGYT DYPFLAPFSR L

Arabidopsis Description

OPR312-oxophytodienoate reductase 3 [Source:UniProtKB/Swiss-Prot;Acc:Q9FUP0]
SUBAcon: [plastid,cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.