Factsheet for PGSC0003DMT400052432

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, plasma membrane

Predictor Summary:

extracellular 5
endoplasmic reticulum 6
vacuole 5
plasma membrane 7
golgi 5
nucleus 1
plastid 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum, plasma membrane
Any Predictor:	nucleus, plastid, secretory
BaCelLo:	secretory
EpiLoc:	nucleus
iPSORT:	secretory
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	plasma membrane
Predotar:	secretory
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	plastid
YLoc:	plasma membrane

PPI

No PPI Data

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc09g013070.2.1	Tomato	endoplasmic reticulum, extracellular	45.95	90.84
PGSC0003DMT400049423	Potato	endoplasmic reticulum, plastid	64.09	69.46
VIT_08s0007g01760.t01	Wine grape	endoplasmic reticulum	67.18	67.7
KRH49269	Soybean	endoplasmic reticulum, plasma membrane	65.64	64.64
Bra017142.1-P	Field mustard	endoplasmic reticulum, plastid	51.35	64.56
KRH27711	Soybean	endoplasmic reticulum, plasma membrane	64.86	63.88
CDY18409	Canola	cytosol, endoplasmic reticulum, plasma membrane	64.09	63.36
CDX93333	Canola	cytosol, endoplasmic reticulum, plasma membrane	63.32	62.84
AT2G38050.1	Thale cress	endoplasmic reticulum	62.93	62.21
GSMUA_Achr4P11670_001	Banana	plasma membrane	57.53	56.02
TraesCS3D01G359100.1	Wheat	endoplasmic reticulum, plasma membrane, plastid, vacuole	48.65	47.37
TraesCS3B01G397800.1	Wheat	endoplasmic reticulum, plasma membrane, plastid	48.26	46.47
OQU87873	Sorghum	plasma membrane	47.1	46.21
TraesCS3A01G366000.1	Wheat	plasma membrane	47.88	45.93
HORVU3Hr1G085400.1	Barley	plasma membrane	47.49	45.39
Zm00001d042843_P001	Maize	endoplasmic reticulum, plasma membrane, vacuole	45.56	45.04
Os01t0851600-01	Rice	endoplasmic reticulum, golgi	45.56	44.03
Os11t0184100-01	Rice	endoplasmic reticulum, plasma membrane, plastid	43.24	41.18
PGSC0003DMT400002569	Potato	extracellular, peroxisome, plasma membrane	22.39	24.17
PGSC0003DMT400002567	Potato	endoplasmic reticulum, extracellular	21.24	20.15
PGSC0003DMT400002570	Potato	endoplasmic reticulum, plasma membrane	20.46	19.92

Protein Annotations

KEGG:00140+1.3.1.22	KEGG:00905+1.3.1.22	Gene3D:1.20.120.1630	EntrezGene:102583495	MapMan:11.3.1.3	InterPro:3-oxo-5-alpha-steroid_4-DH
InterPro:3-oxo-5_a-steroid_4-DH_C	GO:GO:0003674	GO:GO:0003824	GO:GO:0003865	GO:GO:0005575	GO:GO:0005622
GO:GO:0005623	GO:GO:0005737	GO:GO:0006629	GO:GO:0008150	GO:GO:0008152	GO:GO:0008202
GO:GO:0016020	GO:GO:0016021	GO:GO:0016627	GO:GO:0055114	InterPro:IPR001104	UniProt:M1BTE3
PFAM:PF02544	EnsemblPlantsGene:PGSC0003DMG400020353	PGSC:PGSC0003DMG400020353	EnsemblPlants:PGSC0003DMT400052432	PIRSF:PIRSF015596	PFscan:PS50244
PANTHER:PTHR10556	PANTHER:PTHR10556:SF37	TMHMM:TMhelix	UniParc:UPI000296A6E1	RefSeq:XP_006363071.1	SEG:seg

Description

3-oxo-5-alpha-steroid 4-dehydrogenase [Source:PGSC_GENE;Acc:PGSC0003DMG400020353]

Coordinates

chr9:-:545935..546892

Molecular Weight (calculated)

29651.0 Da

IEP (calculated)

9.392

GRAVY (calculated)

0.205

Length

259 amino acids

Sequence
(BLAST)

001: MFSDQSLYHY SLLSLFLIGP PTFLACQFLT APYGKHRRSG WGPTISPPLA WFLMESPTLW LTLILFPFGK NYNNPLSLIL ISPYLFHYTN RTIIYPLKLY
101: FNSKGGSPAS GFPVSIAFMA FVFNLLNAYV QARWVSHYAE YDSDEWFWVR FAGGMVVFAG GMAVNVWADK VLVGLKSEGG GYKIPKGGLF EYVSCPNYLG
201: EIVEWLGWAL MTGSWAGFGF FLYTFANLVP RARFNHEWYL QKFGEDYPRK RKAVIPFLY

Best Arabidopsis Sequence Match ( AT2G38050.1 )
(BLAST)

001: MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP IAWFVMESPT LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR
101: LFRSSFPAGK NGFPITIAAL AFTFNLLNGY IQARWVSHYK DDYEDGNWFW WRFVIGMVVF ITGMYINITS DRTLVRLKKE NRGGYVIPRG GWFELVSCPN
201: YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK WYIAKFKEEY PKTRKAVIPF VY

Arabidopsis Description

DET2Steroid 5-alpha-reductase DET2 [Source:UniProtKB/Swiss-Prot;Acc:Q38944]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.