Factsheet for PGSC0003DMT400060263

Subcellular Localization

min:

: max

Winner_takes_all: cytosol

Predictor Summary:

nucleus 3
cytosol 2
mitochondrion 1
cytoskeleton 1

Predictors

GFP

MS/MS

Papers

cytosol: 28506210
nucleus: 28506210

gfp PMID: 28506210 doi

J Bai, J Mao, H Yang, A Khan, A Fan, S Liu, J Zhang, D Wang, H Gao, J Zhang

College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, Gansu, People's Republic of China., College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, Gansu, People's Republic of China. baijp@gsau.edu.cn., College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, Gansu, People's Republic of China., Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, 730070, Gansu, People's Republic of China., Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, 730070, Gansu, People's Republic of China. baijp@gsau.edu.cn., Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, 730070, Gansu, People's Republic of China. jlzhang@lzu.edu.cn., International Potato Center (CIP), Avenida La Molina 1895, La Molina Apartado, 1558, Lima, Peru., State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, Gansu, People's Republic of China., State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, Gansu, People's Republic of China. jlzhang@lzu.edu.cn.

PPI

Inferred distinct locusB in Crop

locusB	locations
PGSC0003DMT400051416

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G08590.1	PGSC0003DMT400051416	AT1G68050.1	14749489
AT5G08590.1	PGSC0003DMT400051416	AT1G68050.1	17872410

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
Solyc05g056550.2.1	Tomato	cytosol	96.65	97.19
KRH59681	Soybean	endoplasmic reticulum	81.56	83.19
KRH41015	Soybean	endoplasmic reticulum	81.56	83.19
KRH54011	Soybean	cytosol	79.61	82.37
KRH63934	Soybean	endoplasmic reticulum	80.17	82.23
GSMUA_Achr11P...	Banana	cytosol	15.08	81.82
PGSC0003DMT400061170	Potato	cytosol	82.12	81.67
VIT_07s0031g03210.t01	Wine grape	cytosol	80.45	81.13
CDX84430	Canola	cytosol	77.93	79.26
TraesCS2A01G303900.1	Wheat	cytosol	78.49	78.71
TraesCS2B01G320500.1	Wheat	cytosol	78.49	78.71
HORVU2Hr1G075470.2	Barley	cytosol	78.49	78.71
TraesCS2D01G302500.1	Wheat	cytosol, golgi	78.49	78.71
Os04t0432000-02	Rice	cytosol	78.77	78.55
AT5G08590.1	Thale cress	cytosol	77.37	78.47
CDY41527	Canola	cytosol	78.21	78.43
Bra038652.1-P	Field mustard	cytosol	78.21	78.43
EES06943	Sorghum	cytosol	79.05	77.75
AT5G63650.1	Thale cress	cytosol	77.65	77.22
GSMUA_Achr11P...	Banana	cytosol	60.06	77.06
CDX69936	Canola	cytosol	78.49	76.57
Bra009354.1-P	Field mustard	cytosol	78.49	75.95
Os02t0551100-01	Rice	plasma membrane	77.37	75.89
CDX78346	Canola	cytosol	78.21	75.68
CDX81116	Canola	cytosol	77.93	75.41
Bra006010.1-P	Field mustard	cytosol	78.21	75.27
CDY21553	Canola	cytosol	78.49	74.54
KXG26327	Sorghum	cytosol	76.26	72.41
PGSC0003DMT400067426	Potato	cytosol	65.36	69.03
PGSC0003DMT400079214	Potato	cytosol	62.57	66.87
PGSC0003DMT400045810	Potato	cytosol	62.29	66.37
PGSC0003DMT400060762	Potato	cytosol, plastid	62.85	65.41
PGSC0003DMT400066617	Potato	cytosol, nucleus	64.8	64.09
PGSC0003DMT400041670	Potato	cytosol	49.72	59.33
Zm00001d042188_P001	Maize	plastid	25.14	45.0
KRH30137	Soybean	extracellular, plasma membrane	12.57	37.5
Zm00001d047445_P001	Maize	plastid	17.04	28.77

Protein Annotations

Gene3D:1.10.510.10	MapMan:18.4.5.2	Gene3D:3.30.200.20	ProteinID:AFR68943	ProteinID:AFR68943.1	GO:GO:0000166
GO:GO:0003674	GO:GO:0003824	GO:GO:0004672	GO:GO:0004674	GO:GO:0005488	GO:GO:0005524
GO:GO:0006464	GO:GO:0006468	GO:GO:0008150	GO:GO:0008152	GO:GO:0009987	GO:GO:0016301
GO:GO:0016310	GO:GO:0016740	GO:GO:0019538	InterPro:IPR000719	EMBL:JX280916	InterPro:Kinase-like_dom_sf
UniProt:M1C5H7	PFAM:PF00069	EnsemblPlantsGene:PGSC0003DMG400023441	PGSC:PGSC0003DMG400023441	EnsemblPlants:PGSC0003DMT400060263	PIRSF:PIRSF000654
ScanProsite:PS00107	ScanProsite:PS00108	PFscan:PS50011	PANTHER:PTHR24343	PANTHER:PTHR24343:SF161	InterPro:Prot_kinase_dom
InterPro:Protein_kinase_ATP_BS	SMART:SM00220	SUPFAM:SSF56112	InterPro:Ser/Thr_kinase_AS	UniParc:UPI000295B200	SEG:seg

Description

Osmotic stress-activated protein kinase [Source:PGSC_GENE;Acc:PGSC0003DMG400023441]

Coordinates

chr5:+:51985882..51989780

Molecular Weight (calculated)

41167.9 Da

IEP (calculated)

6.615

GRAVY (calculated)

-0.592

Length

358 amino acids

Sequence
(BLAST)

001: MQNYEVVKEL GSGNFGVARL MRHKETKQLV AMKYIERGRK IDENVAREII NHRSLRHPNI IRFKEVLLTS THLGIVMEYA AGGELFDRIC QAGRFSEPEA
101: RYFFQQLISG VHYCHNMQIC HRDLKLENTL LDGSPAPRLK ICDFGYSKSS VLHSRPKSTV GTPAYIAPEV LSRREYDGKS ADVWSCGVTL YVMLVGGYPF
201: EDVDDPKNFR KTISRIMGVQ YKIPDYVHIS HDCKHLLSRI FVASPARRIT LKEIKNHPWF LKSLPKELTE SAQAVYYKRD NPTFSLQSIE EIMKIVSEAR
301: NPPPPSRPVP HFGWGTEEEE EDGETKEEDA EEDEEDEYEK QVKQVHASGE FHITHDDA

Best Arabidopsis Sequence Match ( AT5G63650.1 )
(BLAST)

001: MDKYEVVKDL GAGNFGVARL LRHKETKELV AMKYIERGRK IDENVAREII NHRSLRHPNI IRFKEVILTP THLAIVMEYA SGGELFERIC NAGRFSEAEA
101: RYFFQQLICG VDYCHSLQIC HRDLKLENTL LDGSPAPLLK ICDFGYSKSS LLHSRPKSTV GTPAYIAPEV LSRREYDGKH ADVWSCGVTL YVMLVGGYPF
201: EDPDDPRNFR KTIQRIMAVQ YKIPDYVHIS QECRHLLSRI FVTNSAKRIT LKEIKKHPWY LKNLPKELTE PAQAAYYKRE TPSFSLQSVE DIMKIVGEAR
301: NPAPSSNAVK GFDDDEEDVE DEVEEEEEEE EEEEEEEEEE EDEYEKHVKE AHSCQEPPKA

Arabidopsis Description

SRK2HSerine/threonine-protein kinase SRK2H [Source:UniProtKB/Swiss-Prot;Acc:Q9FFP9]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.