CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum

Predictor Summary:

cytosol 1
mitochondrion 1
endoplasmic reticulum 5
plasma membrane 3
extracellular 2
vacuole 2
golgi 2

Predictors

GFP

MS/MS

Papers

Winner Takes All:	endoplasmic reticulum
Any Predictor:	cytosol, mitochondrion, secretory
BaCelLo:	cytosol
iPSORT:	mitochondrion
MultiLoc:	endoplasmic reticulum
Plant-mPloc:	plasma membrane
PProwler:	secretory
TargetP:	secretory
WoLF PSORT:	endoplasmic reticulum
YLoc:	endoplasmic reticulum

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH22653
KRH26664

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT3G48890.1	KRH22653	AT5G22290.1	21798944
AT3G48890.1	KRH26664	AT5G22290.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH35342	Soybean	endoplasmic reticulum	84.0	80.43
KRH09074	Soybean	endoplasmic reticulum	70.22	71.17
KRH38500	Soybean	endoplasmic reticulum	68.89	70.45
VIT_16s0100g01280.t01	Wine grape	endoplasmic reticulum	60.0	62.79
KRH62716	Soybean	mitochondrion	21.78	62.03
Bra018028.1-P	Field mustard	endoplasmic reticulum	60.89	61.71
Solyc03g025950.2.1	Tomato	plastid	61.33	60.79
PGSC0003DMT400037158	Potato	mitochondrion	61.33	60.79
CDY37905	Canola	cytosol	34.67	59.09
Bra019571.1-P	Field mustard	cytosol	34.67	59.09
AT3G48890.1	Thale cress	endoplasmic reticulum	60.89	58.8
KRH61642	Soybean	cytosol	22.22	50.0
KRG94134	Soybean	endoplasmic reticulum	43.56	49.0
KRH31695	Soybean	cytosol	16.89	48.1
KRH52307	Soybean	nucleus	22.67	45.95
KRH75821	Soybean	cytosol, nucleus, plastid	16.89	45.24
TraesCS1D01G165500.1	Wheat	golgi, plastid	48.89	45.08
TraesCS1A01G173200.3	Wheat	golgi, plastid	47.11	43.44
TraesCS1B01G191100.2	Wheat	plastid	47.11	43.44
Os10t0502500-04	Rice	plastid	46.67	39.62
EER93976	Sorghum	plastid	45.33	39.38
KRH25811	Soybean	mitochondrion	16.0	36.73
HORVU1Hr1G045610.5	Barley	plastid	46.22	36.36
KRH17625	Soybean	endoplasmic reticulum, golgi, vacuole	20.0	18.67
KRG90070	Soybean	endoplasmic reticulum	19.11	17.84

Protein Annotations

EntrezGene:100788825	MapMan:11.3.2.1.4	Gene3D:3.10.120.10	EMBL:ACUP02012689	InterPro:Cyt_B5-like_heme/steroid-bd	InterPro:Cyt_B5-like_heme/steroid_sf
EnsemblPlantsGene:GLYMA_20G157300	GO:GO:0003674	GO:GO:0005488	GO:GO:0005575	GO:GO:0005623	GO:GO:0012505
GO:GO:0016020	GO:GO:0016021	GO:GO:0020037	UniProt:I1NGS1	InterPro:IPR001199	InterPro:IPR036400
EnsemblPlants:KRG91473	ProteinID:KRG91473	ProteinID:KRG91473.1	PFAM:PF00173	PANTHER:PTHR10281	PANTHER:PTHR10281:SF62
SMART:SM01117	SUPFAM:SSF55856	TMHMM:TMhelix	UniParc:UPI000233DE6D	SEG:seg	:

Description

hypothetical protein

Coordinates

chr20:-:39624093..39627015

Molecular Weight (calculated)

24873.1 Da

IEP (calculated)

4.240

GRAVY (calculated)

-0.407

Length

225 amino acids

Sequence
(BLAST)

001: MAVQLWETLK EAIVAYTGFS PSTFFTVLAL LFAVYYVVTG LFGSSDDHHH RHRHVEEEEE MPPLRPPVQL GEITAEELKA YDGTDPEKPL LMAIKAQIYD
101: VSQSRMFYGP GGPYALFAGK DASRALAKMS FEEKDLTGDI SGLGPFELDA LQDWEYKFMS KYVKVGTVKS EEVPVTEPES IGEPLESTSR DIDAAAKPTD
201: TEDGKLETPA VKSDETPSNV DADKE

Best Arabidopsis Sequence Match ( AT5G52240.1 )
(BLAST)

001: MALELWQTLK EAIHAYTGLS PVVFFTALAL AFAIYQVISG WFASPFDDVN RHQRARSLAQ EEEPPIPQPV QVGEITEEEL KQYDGSDPQK PLLMAIKHQI
101: YDVTQSRMFY GPGGPYALFA GKDASRALAK MSFEEKDLTW DVSGLGPFEL DALQDWEYKF MSKYAKVGTV KVAGSEPETA SVSEPTENVE QDAHVTTTPG
201: KTVVDKSDDA PAETVLKKEE

Arabidopsis Description

MSBP1Membrane steroid-binding protein 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9XFM6]
SUBAcon: [endoplasmic reticulum]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.