CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

nucleus 4
plastid 1
mitochondrion 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	mitochondrion, nucleus, plastid
BaCelLo:	nucleus
MultiLoc:	nucleus
Plant-mPloc:	plastid
PProwler:	mitochondrion
WoLF PSORT:	nucleus
YLoc:	nucleus

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH19789
KRH23181
KRH32381

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT5G02820.1	KRH23181	AT3G20780.1	11410368
AT5G02820.1	KRH23181	AT3G20780.1	16339310
AT5G02820.1	KRH19789	AT5G02820.1	16339310
AT5G02820.1	KRH32381	AT5G02820.1	16339310
AT5G02820.1	KRH19789	AT5G02820.1	21798944
AT5G02820.1	KRH32381	AT5G02820.1	21798944

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH32381	Soybean	nucleus	99.28	98.81
VIT_08s0040g03250.t01	Wine grape	nucleus	87.77	87.14
PGSC0003DMT400071721	Potato	nucleus	83.69	83.29
AT5G02820.1	Thale cress	nucleus	84.65	82.67
CDX70343	Canola	nucleus	84.41	82.44
Bra009564.1-P	Field mustard	nucleus	84.65	82.28
CDY10421	Canola	nucleus	84.17	82.2
Solyc09g007210.2.1	Tomato	nucleus	49.4	76.87
EER95053	Sorghum	plastid	70.74	67.66
Os03t0284800-01	Rice	nucleus	71.46	67.42
Zm00001d028705_P001	Maize	plastid	70.26	67.2
TraesCS4B01G198400.1	Wheat	nucleus, plastid	69.3	65.53
TraesCS4D01G199200.1	Wheat	nucleus, plastid	69.3	65.53
TraesCS4A01G105600.1	Wheat	nucleus	69.06	65.31
HORVU4Hr1G057310.1	Barley	nucleus, plastid	68.82	60.42
KRH73898	Soybean	cytosol	27.34	31.06
KRH27888	Soybean	nucleus, plasma membrane, plastid	26.62	29.06

Protein Annotations

Gene3D:1.10.10.10	EntrezGene:100778158	Gene3D:3.40.1360.10	MapMan:35.1	EMBL:ACUP02008206	EnsemblPlantsGene:GLYMA_13G136100
GO:GO:0000166	GO:GO:0000287	GO:GO:0000737	GO:GO:0003674	GO:GO:0003676	GO:GO:0003677
GO:GO:0003824	GO:GO:0003916	GO:GO:0003918	GO:GO:0005488	GO:GO:0005524	GO:GO:0005575
GO:GO:0005622	GO:GO:0005623	GO:GO:0005634	GO:GO:0005694	GO:GO:0006139	GO:GO:0006259
GO:GO:0006265	GO:GO:0008150	GO:GO:0008152	GO:GO:0009056	GO:GO:0009330	GO:GO:0009987
GO:GO:0016043	GO:GO:0016787	GO:GO:0016853	GO:GO:0046872	GO:GO:0061505	UniProt:I1LYZ8
InterPro:IPR036388	EnsemblPlants:KRH19789	ProteinID:KRH19789	ProteinID:KRH19789.1	HAMAP:MF_00132	PFAM:PF04406
PRINTS:PR01550	PRINTS:PR01552	PANTHER:PTHR10848	PANTHER:PTHR10848:SF0	SUPFAM:SSF56726	InterPro:Spo11/TopoVI_A
InterPro:Spo11/TopoVI_A_N	InterPro:Spo11/TopoVI_A_sf	InterPro:TOPRIM_Topo6A/Spo11	InterPro:TopoVI_A	UniParc:UPI000233AC4D	InterPro:WH-like_DNA-bd_sf
SEG:seg	:	:	:	:	:

Description

hypothetical protein

Coordinates

chr13:+:24861630..24864623

Molecular Weight (calculated)

46732.8 Da

IEP (calculated)

8.685

GRAVY (calculated)

-0.188

Length

417 amino acids

Sequence
(BLAST)

001: MADNKKRRRK DPAGDELPFK NKLKPDSAIM ETLKQFSASS SSSSVSQTLT LQDLSIPFRC REVADLSLSS VQSNIESLIL RIAHSILSGH GFSFDVPSRS
101: AANQLYVPEL DRIVLKDKSS LRPFANISTV RKSAITARIL QLIHQLCIKG IHVTKRDLFY TDVKLFQDQI QSDAVLDDVS CMLGCTRSSL NVVAAEKGVV
201: VGRLIFSDNG DMIDCTKMGM GGKAIPPNID RVGDMQSDAL FILLVEKDAA YMRLAEDRFY NRFPCIIVTA KGQPDVSTRL FLRKMKTELK LPVLALVDSD
301: PYGLKILSVY GCGSKNMSYD SANLTTPDIK WLGVRPSDLD KYKIPEQCRL PMTEQDIKTG KDLLEEDFVK KNPGWVEELT LMVKTKQKAE IQALSTFGFQ
401: YLSEVYLPLK LQQKDWL

Best Arabidopsis Sequence Match ( AT5G02820.1 )
(BLAST)

001: MADKKKRKRS KDDEAEELPF KSILESDDVI TELLKSYISS SIKAAAGAGG ASSSSSKPLT LADLSLSSSC REVADLSLSS VQTEIETVIV QIARSILAGD
101: GFSFSVPSRA ASNQLYVPEL DRIVLKDKST LRPFASVSSV RKTTITTRIL ALIHQLCLRN IHVTKRDLFY TDVKLFQDQT QSDAVLDDVS CMLGCTRSSL
201: NVIAAEKGVV VGRLIFSDNG DMIDCTKMGM GGKAIPPNID RVGDMQSDAM FILLVEKDAA YMRLAEDRFY NRFPCIIVTA KGQPDVATRL FLRKMKMELK
301: LPVLALVDSD PYGLKILSVY GCGSKNMSYD SANLTTPDIK WLGIRPSDLD KYKIPEQCRL PMTEQDIKTG KDMLEEDFVK KNPGWVEELN LMVKTKQKAE
401: IQALSSFGFQ YLSEVYLPLK LQQQDWL

Arabidopsis Description

TOP6ADNA topoisomerase 6 subunit A [Source:UniProtKB/TrEMBL;Acc:A0A178UQD3]
SUBAcon: [nucleus]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.