CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: endoplasmic reticulum, nucleus

Predictor Summary:

cytosol 2
peroxisome 3
mitochondrion 1
plastid 2

Predictors

GFP

MS/MS

Papers

nucleus: 21132161
endoplasmic reticulum: 27224218
endoplasmic reticulum: 28499913
mitochondrion: 28499913
nucleus: 28499913

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

msms PMID: 28499913 doi

S Komatsu, X Wang, X Yin, Y Nanjo, H Ohyanagi, K Sakata

Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan. Electronic address: ksakata@maebashi-it.ac.jp., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan., Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan. Electronic address: komatsu.setsuko.fu@u.tsukuba.ac.jp., King Abdullah University of Science and Technology (KAUST), Computational Bioscience Research Center (CBRC), Thuwal 23955-6900, Saudi Arabia.

msms PMID: 27224218 doi

X Wang, S Komatsu

Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan., National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH56371
KRH62568

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT2G33150.1	KRH56371	AT5G56290.1	24879895
AT2G33150.1	KRH62568	AT5G56290.1	24879895

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRG90241	Soybean	nucleus	97.41	97.83
AT2G33150.1	Thale cress	cytosol	84.67	84.85
CDY19452	Canola	cytosol	84.67	84.67
Bra005522.1-P	Field mustard	cytosol	84.23	84.42
CDY17140	Canola	cytosol	84.23	84.42
VIT_05s0051g00720.t01	Wine grape	cytosol	83.8	84.16
Solyc09g091470.2.1	Tomato	plastid	84.45	84.09
PGSC0003DMT400076175	Potato	cytosol	84.45	84.09
Bra022927.1-P	Field mustard	cytosol	83.15	83.7
CDX84701	Canola	cytosol	83.37	83.55
CDX79511	Canola	cytosol	83.15	83.33
CDY23766	Canola	cytosol	82.94	83.12
Bra021829.1-P	Field mustard	cytosol	82.72	82.9
CDY37750	Canola	mitochondrion	82.72	82.9
CDX86611	Canola	cytosol	80.56	82.71
AT1G04710.1	Thale cress	cytosol	78.83	82.39
Bra030586.1-P	Field mustard	mitochondrion	79.48	81.6
CDY15520	Canola	mitochondrion	79.48	81.6
KRH49796	Soybean	endoplasmic reticulum	72.35	75.28
KRG89223	Soybean	mitochondrion	71.49	74.38
KRH14066	Soybean	endoplasmic reticulum, nucleus	28.94	32.92
KRH74050	Soybean	endoplasmic reticulum	28.73	32.68
KRH01946	Soybean	nucleus	27.86	31.54
KRH51203	Soybean	cytosol, mitochondrion, peroxisome	27.86	31.54

Protein Annotations

EntrezGene:100170734	Gene3D:3.40.47.10	MapMan:5.7.3.2.3	EMBL:AB333797	EMBL:ACUP02006501	UniProt:B0M1A9
EnsemblPlantsGene:GLYMA_10G124700	GO:GO:0003674	GO:GO:0003824	GO:GO:0008150	GO:GO:0008152	GO:GO:0016740
GO:GO:0016746	GO:GO:0016747	InterPro:IPR016039	EnsemblPlants:KRH33469	ProteinID:KRH33469	ProteinID:KRH33469.1
PFAM:PF00108	PFAM:PF02803	ScanProsite:PS00098	ScanProsite:PS00099	ScanProsite:PS00737	PANTHER:PTHR43853
PANTHER:PTHR43853:SF4	SUPFAM:SSF53901	TIGRFAMs:TIGR01930	InterPro:Thiolase	InterPro:Thiolase-like	InterPro:Thiolase_AS
InterPro:Thiolase_C	InterPro:Thiolase_CS	InterPro:Thiolase_N	InterPro:Thiolase_acyl_enz_int_AS	UniParc:UPI000165BB5E	SEG:seg

Description

hypothetical protein

Coordinates

chr10:-:32501982..32507559

Molecular Weight (calculated)

48619.4 Da

IEP (calculated)

7.875

GRAVY (calculated)

-0.027

Length

463 amino acids

Sequence
(BLAST)

001: MEKAINRQKI LLHHLNPSSS THPNESSSLH ASACVAGDSA AYQRTSTFGD DVVIVAAYRT AHCKAKRGGF KDTLPDDLLA PVLKAVIEKT NVNPSEVGDI
101: VVGSVLAPGA QRASECRMAA FYAGFPETVP VRTVNRQCSS GLQAVADVAA AIRAGFYDIG IGAGLESMTT NPMGWDGSVN PKVKMFEQAQ NCLLPMGITS
201: ENVAQRFGVS RKEQDQAAVE SHRRAAAATA AGKFKDEIVP VTTKIVDPKT GEEKSVTISV DDGIRPGTTV SDLGRLKPVF KKDGSTTAGN SSQVTDGASA
301: VLLMKRSVAL QKGLPILGVF RTFAAVGVDP AIMGVGPAAA IPVAVKAAGL ELDDIDLFEI NEAFASQFVY CRNKLGLDPE KINVNGGAMA IGHPLGSTGA
401: RCVATLLHEM KKRGRDCRFG VISMCIGTGM GAAAVFESGD CADELCNARK VDDLLLSKDA RLK

Best Arabidopsis Sequence Match ( AT2G33150.1 )
(BLAST)

001: MEKAIERQRV LLEHLRPSSS SSHNYEASLS ASACLAGDSA AYQRTSLYGD DVVIVAAHRT PLCKSKRGNF KDTYPDDLLA PVLRALIEKT NLNPSEVGDI
101: VVGTVLAPGS QRASECRMAA FYAGFPETVA VRTVNRQCSS GLQAVADVAA AIKAGFYDIG IGAGLESMTT NPMAWEGSVN PAVKKFAQAQ NCLLPMGVTS
201: ENVAQRFGVS RQEQDQAAVD SHRKAAAATA AGKFKDEIIP VKTKLVDPKT GDEKPITVSV DDGIRPTTTL ASLGKLKPVF KKDGTTTAGN SSQVSDGAGA
301: VLLMKRSVAM QKGLPVLGVF RTFAAVGVDP AIMGIGPAVA IPAAVKAAGL ELDDIDLFEI NEAFASQFVY CRNKLGLDPE KINVNGGAMA IGHPLGATGA
401: RCVATLLHEM KRRGKDCRFG VVSMCIGTGM GAAAVFERGD GVDELRNARK VEAQGLLSKD AR

Arabidopsis Description

PED1PKT3 [Source:UniProtKB/TrEMBL;Acc:A0A178VRP9]
SUBAcon: [cytosol]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.