CropPAL

Subcellular Localization

min:

: max

Winner_takes_all: nucleus

Predictor Summary:

plastid 9
nucleus 1
extracellular 1
endoplasmic reticulum 1
vacuole 1
plasma membrane 1
golgi 1
peroxisome 1

Predictors

GFP

MS/MS

Papers

Winner Takes All:	nucleus
Any Predictor:	nucleus, peroxisome, plastid, secretory
BaCelLo:	plastid
ChloroP:	plastid
iPSORT:	plastid
MultiLoc:	plastid
Plant-mPloc:	nucleus, plastid
Predotar:	secretory
PProwler:	plastid
PTS1:	peroxisome
TargetP:	plastid
WoLF PSORT:	plastid
YLoc:	plastid

nucleus: 21132161

msms PMID: 21132161 doi

B Cooper, KB Campbell, J Feng, WM Garrett, R Frederick

Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD 20705, USA. bret.cooper@ars.usda.gov

PPI

Inferred distinct locusB in Crop

locusB	locations
KRH10294
KRH23029
KRH24840
KRH29844

Inferred from Arabidopsis experimental PPI

Ath locusA	locusB	Ath locusB	Paper
AT4G29400.1	KRH10294	AT3G46090.1	17259181
AT4G29400.1	KRH23029	AT3G46090.1	17259181
AT4G29400.1	KRH24840	AT3G46090.1	17259181
AT4G29400.1	KRH29844	AT3G46090.1	17259181

Homology

Paralog

locus	Identity	Homology Identity

Ortholog

locus	Homology Species	Location	Identity	Homology Identity
KRH44778	Soybean	plastid	79.19	81.21
VIT_11s0016g02630.t01	Wine grape	cytosol	48.14	77.89
Solyc10g045310.1.1	Tomato	nucleus	50.0	70.31
PGSC0003DMT400074006	Potato	cytosol, mitochondrion	52.8	57.05
GSMUA_Achr8P24980_001	Banana	plastid	53.42	52.76
Os01t0558800-01	Rice	plastid	45.96	52.67
TraesCS3D01G206900.1	Wheat	plastid	44.72	52.17
CDY43498	Canola	plastid	47.83	52.03
CDY06810	Canola	plastid	48.14	52.01
Bra011105.1-P	Field mustard	plastid	47.52	51.69
Zm00001d011166_P001	Maize	plastid	45.65	50.87
EES06656	Sorghum	plastid	45.65	50.17
AT4G29400.1	Thale cress	plastid	46.27	49.34
TraesCSU01G050100.1	Wheat	plastid	32.61	46.88
HORVU3Hr1G048210.5	Barley	plastid	31.99	36.27
KRG93163	Soybean	nucleus	29.5	31.56
KRH64843	Soybean	nucleus	28.88	30.59

Protein Annotations

EMBL:ACUP02005477	EnsemblPlants:KRH37622	EnsemblPlantsGene:GLYMA_09G078300	EntrezGene:100812461	InterPro:DUF3531	PANTHER:PTHR33102
PANTHER:PTHR33102:SF2	PFAM:PF12049	ProteinID:KRH37622	ProteinID:KRH37622.1	SEG:seg	UniParc:UPI0002337D0D
UniProt:I1L1Y0	MapMan:35.2	:	:	:	:

Description

hypothetical protein

Coordinates

chr9:-:8708549..8718528

Molecular Weight (calculated)

36366.7 Da

IEP (calculated)

4.836

GRAVY (calculated)

-0.514

Length

322 amino acids

Sequence
(BLAST)

001: MIVLHLFAAP LPSCTTITCS KHFSHDNHFV RSSTVPRLSL SHVRTRIFLC SARNRQKDLN GRDFSIDIVK EDQELEDDGD DGFLAQGMYA RRDAKDIDEE
101: EAQENEDDDS GYSGRGPYTG RPGKDYDRDP ELGNILGSFL EDPQAAQSKL EDRLRKKRSK ILHTKTGSGK PMKVSFNKFD FSNSYIWFEF YNVPLTKDVS
201: LICDTIRAWH IIGRLGGCNA MNMQLSQSPM DARPSYDYIQ GANVEPTTFY NIGDLELQDN LARIWVDIGT VEPLLLDVLI NALTQISSDF VGIKQVMFGG
301: SEFENWNDSL KSEDAGYGVH KI

Best Arabidopsis Sequence Match ( AT4G29400.1 )
(BLAST)

001: MLRFQCFLHL LQPSMAKNNS NHSNICLHNL APFRVTDTIP VQKIQELRAN RSRESGKLRV NGIGREMEEG VEEFEEVDDG DDDEVEDEFS AKKRGVYRAK
101: KEKIDYDKDP EFADILGDCL DNPDKAQKKM EERLRKKRNK ILHTKTGSAT SMPVTFNKFE YSNSYMWLEF YNTPLDKDIA LISDTIRSWH ILGRLGGYNS
201: MNMQLSQAPL DKRPNYDAIL GANVEPTTFY NIGDLEVQDN VARIWLDIGT SEPLILDVLI NALTQISSDY VGIKKVVFGG SEFESWKENM TSEESGFRVH
301: KI

Arabidopsis Description

Oxidoreductase/transition metal ion-binding protein (DUF3531) [Source:UniProtKB/TrEMBL;Acc:Q5XV66]
SUBAcon: [plastid]

Hydropathy Plot

About CropPAL

The Protein Annotated Locations Database (CropPAL) houses large scale proteomic and GFP localization data from published experimental studies in Soybean (Glycine max), Maize (Zea mays), Wheat (Triticum aestivum), Barley (Hordeum vulgare), Rice (Oryza sativa), Field mustard (Brassica rapa), Canola (Brassica napus), Sorghum (Sorghum bicolor), Potato (Solanum tuberosum), Tomato (Solanum lycopersicum), Banana (Musa acuminata) and Wine grape (Vitis vinifera) as well as precomputed predictions for protein subcellular localizations using protein sequences.